Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.93.3% 240.1 / 13.6 60pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base . (28/28);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01
*NC_0009132,173,3620C.94.9% 236.7 / 9.2 60pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base . (28/28);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTA  >  NC_000913/2173216‑2173503
                                                                                                                                                 ||                                                                                                                                             
aaCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAATATGGTGAGTGTGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                             >  1:667981/1‑147 (MQ=255)
aaCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                             >  2:979287/1‑147 (MQ=255)
     aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgtat                                                                                                                                        >  1:1053576/1‑142 (MQ=255)
      tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATg                                                                                                                                     >  1:50832/1‑149 (MQ=255)
        tcaaCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTc                                                                                                                                   <  2:276269/146‑1 (MQ=255)
          cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTccc                                                                                                                                 <  2:342447/149‑1 (MQ=255)
          cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCCCCGCCGTATGTccc                                                                                                                                 >  1:109958/1‑149 (MQ=255)
                  aCGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACAA‑‑CCGCCGTTAGTCCCAGCAGCaa                                                                                                                         >  2:854026/1‑149 (MQ=255)
                    ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCAGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAACTCAGGCTTGCCGATCCCA‑‑CCGCCGTAAGTCCCAGCCGCAACg                                                                                                                       >  2:604234/1‑149 (MQ=255)
                        aCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCgg                                                                                                                   <  1:37844/149‑1 (MQ=255)
                              aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCATACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg                                                                                                             <  1:1178072/149‑1 (MQ=255)
                              aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg                                                                                                             <  2:374740/149‑1 (MQ=255)
                                cgcgACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                           <  2:926247/149‑1 (MQ=255)
                                cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAAGTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                           <  2:903425/149‑1 (MQ=255)
                                  ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                         >  2:613594/1‑149 (MQ=255)
                                  ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGGGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                         >  2:646553/1‑149 (MQ=255)
                                    aCGAAGCAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGCAATTAAAATGGTGAGAGGGATAAAAATCAGGCTTGCCGATACCA‑‑ACGCCGTAGGTCCCAGCAGCAACGACGGATAAAGGCCAAt                                                                                                       >  2:560279/1‑149 (MQ=255)
                                      gaagaaGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACACGGCACACAAACAGCATTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa                                                                                                     >  1:180357/1‑149 (MQ=255)
                                       aagaagCCGATGGGGGCAAGCTCGACTAACGGCAGCACCTGATTACCCGTCACACTAACATCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                                    <  2:1237592/149‑1 (MQ=255)
                                        agaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                                   <  1:26330/149‑1 (MQ=255)
                                        agaagCCGATGGTGGCAACATCGCCAAACGGCAGCACCTGATTACCCGTCTCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTACCGATACCA‑‑CCGCCGTATGACCCAGCAGCATCTCCTGATCAAGGCAAATCAga                                                                                                   >  1:919509/1‑148 (MQ=255)
                                             ccGATGGTGGCAAGATCGCCAACCGGCAGCCCCTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                              >  1:872231/1‑149 (MQ=255)
                                              cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGCGTGGGATAAAAATCAGGCTGGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc                                                                                             >  1:1015371/1‑149 (MQ=255)
                                               gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                            <  2:231143/149‑1 (MQ=255)
                                                   gtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACCAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACTCCGGATCAAGGCCCATCAGGAACTCCTGAcc                                                                                        >  2:1129907/1‑149 (MQ=255)
                                                   gtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc                                                                                        >  2:403240/1‑149 (MQ=255)
                                                         aGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGAGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGCTCAAGGACCTTCAGGAGCTCCTGACCGCCGaa                                                                                  >  1:1172654/1‑149 (MQ=255)
                                                             cGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                              <  1:1201927/149‑1 (MQ=255)
                                                              gCCACACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAACAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGAAATCCTGACCGCCGCACTTCg                                                                             >  2:744090/1‑149 (MQ=255)
                                                               ccAAACGGCAGCACCTGATTACCCGGCACACAACCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTGTGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc                                                                            <  1:710280/149‑1 (MQ=255)
                                                                   aCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGt                                                                        <  2:594428/149‑1 (MQ=255)
                                                                       cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc                                                                    <  1:328403/149‑1 (MQ=255)
                                                                            ccTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                               >  1:1224550/1‑149 (MQ=255)
                                                                               gATTCCGCGGCACACACACAGCAAATAAAATGCGGAGTGGGTTATAAATCAGGCTTGCCGATACCA‑‑CCGCCGTAGGGCCCTGCAGCAACTCCGTATCAAGGCCAATCAGCAACTCCTGACCGCCGAACTTCGCCTGTATACGACTACGa                                                            <  2:1177848/149‑1 (MQ=255)
                                                                               gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCTGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa                                                            >  2:899148/1‑149 (MQ=255)
                                                                                aTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg                                                           <  2:743973/149‑1 (MQ=255)
                                                                                    cccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGCCGACGAGCCTg                                                       >  1:925762/1‑149 (MQ=255)
                                                                                     ccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGc                                                      <  2:463085/149‑1 (MQ=255)
                                                                                      cGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCt                                                     >  1:112273/1‑149 (MQ=255)
                                                                                             caAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACTA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                              >  2:662191/1‑149 (MQ=255)
                                                                                              aaaCAGCAATTAAAATGGTGAGTGGGATAAAAATCATGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGCTCACGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAACCGCCTACGAGCCTGCTTAGCGATg                                             >  2:993639/1‑149 (MQ=255)
                                                                                                aCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATggg                                           <  2:971737/149‑1 (MQ=255)
                                                                                                   gCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCGGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt                                        <  1:572600/149‑1 (MQ=255)
                                                                                                       ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCCTGCTTAGCGATGGGCGTTaaa                                    >  1:862940/1‑149 (MQ=255)
                                                                                                       ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                    <  1:979287/149‑1 (MQ=255)
                                                                                                       ttAAAATGGTGAGGGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                    <  2:1053576/149‑1 (MQ=255)
                                                                                                          aaaTGGTGAGTGGGATTAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGCTCAAGGCCTATCAGGAACTCCTGACCGCCGAACTTCGCCTGTATACGACTACGATCATGCTTACCGATGGGCGTTAAAaca                                 >  2:1069527/1‑149 (MQ=255)
                                                                                                           aaTGGTGAGTGGGATAATAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                <  1:201752/149‑1 (MQ=255)
                                                                                                           aaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCATGCTTAGCGATGGGCGTTAAAccat                                >  1:868554/1‑149 (MQ=255)
                                                                                                               gTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatccat                            >  1:1077368/1‑149 (MQ=255)
                                                                                                               gTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatccat                            >  1:1072416/1‑149 (MQ=255)
                                                                                                                      ggATAAAAATCCGCCTTGCCGATAC‑‑CTGCGCCGGATGTCCCATCAGCAACGCCGGATCAAGGCCAATCAGGAAGTCCTGACCTCCGATCTTCGCCTGTAAACGACTCCGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGt                     <  1:800768/149‑1 (MQ=255)
                                                                                                                      ggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGt                     <  2:50832/149‑1 (MQ=255)
                                                                                                                         tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGACGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGACTACGATCCTGCTTAGCGATGGGCGTTAACCCATCCATGATGGGTTTa                  >  2:925183/1‑149 (MQ=255)
                                                                                                                          aaaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCATCAGCAACGCCGGATCAATGCCAATCAGCAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa                 <  1:604234/149‑1 (MQ=255)
                                                                                                                              aTCAGGCTTGCCGATACCACCC‑‑CCGGATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc             <  2:109958/149‑1 (MQ=255)
                                                                                                                               tCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAATCGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGCTGGGTTTATTCAcc            >  2:396380/1‑149 (MQ=255)
                                                                                                                                  ggCTTCCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCCAGGCCAATCAGCAACTCGTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGt         <  2:262887/149‑1 (MQ=255)
                                                                                                                                   gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTg        >  2:1166649/1‑149 (MQ=255)
                                                                                                                                    cTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGTGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg       <  2:872231/149‑1 (MQ=255)
                                                                                                                                       gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAGCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGTGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt    <  2:758812/149‑1 (MQ=255)
                                                                                                                                         cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGTCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTa  <  1:646553/149‑1 (MQ=255)
                                                                                                                                         cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTa  >  2:38333/1‑149 (MQ=255)
                                                                                                                                                 ||                                                                                                                                             
AACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTA  >  NC_000913/2173216‑2173503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: