Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,406,129	T→C	intergenic (+144/‑61)	purA → / → nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,406,129	0	T	C	100.0%	220.3 / NA	75	intergenic (+144/‑61)	purA/nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/37);  total (38/37)

AATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTT  >  NC_000913/4405984‑4406274                                                                                                                                                  |                                                                                                                                                  aaTTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCtgt                                                                                                                                                <  1:859338/149‑1 (MQ=255)   ttCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTgg                                                                                                                                              >  2:455954/1‑149 (MQ=255)        gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGt                                                                                                                                         <  1:1101646/149‑1 (MQ=255)        gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGt                                                                                                                                         <  1:1229811/149‑1 (MQ=255)             ccTGGCAGATATTTTGCCGGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatc                                                                                                                                    <  2:613772/149‑1 (MQ=255)              cTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatca                                                                                                                                   <  1:998595/149‑1 (MQ=255)              cTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatca                                                                                                                                   >  2:997253/1‑149 (MQ=255)                    gATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAata                                                                                                                             >  1:543037/1‑149 (MQ=255)                      tatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTAGGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTGCTTCAGAGTACTGTAATAAATTGGCGAGACAGCCGTTGGGTGGTTTTGCTTCAattga                                                                                                                           >  1:1164186/1‑146 (MQ=255)                       atTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCCGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGATTTTTTCCTTCCCCGAACTGAAATAAATAAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaa                                                                                                                          >  1:928799/1‑149 (MQ=255)                        ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                         <  2:1183197/149‑1 (MQ=255)                         tttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAAt                                                                                                                        <  1:333213/149‑1 (MQ=255)                                gccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAATTAAATTTGCGCCACAGCCTGGGGCTGGTTTATCATCAATATAAATGTAtttt                                                                                                                 >  2:1074487/1‑149 (MQ=255)                                                  tACATTGCTGTGTCGGTTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTg                                                                                               <  1:386181/149‑1 (MQ=255)                                                   aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                                                                              >  1:1231435/1‑149 (MQ=255)                                                    cATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAg                                                                                             >  2:412103/1‑149 (MQ=255)                                                     aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                                                                            >  1:323714/1‑149 (MQ=255)                                                     aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                                                                            >  2:489093/1‑149 (MQ=255)                                                     aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                                                                            <  2:915088/149‑1 (MQ=255)                                                      ttGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGt                                                                                           <  2:543037/149‑1 (MQ=255)                                                      ttGATGTGTCGGTTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCTCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGt                                                                                           <  2:1048665/149‑1 (MQ=255)                                                        tcTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTg                                                                                         <  1:997253/148‑1 (MQ=255)                                                          tgtgTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                                                                       <  2:729963/149‑1 (MQ=255)                                                          tgtgTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                                                                       <  1:399351/149‑1 (MQ=255)                                                          tgtgTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                                                                       <  1:1018166/149‑1 (MQ=255)                                                            tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGATGCTTTATCATCAATATAAATGTATTTTTTCCCGATTTCACTTTTGAGGTTGAtgt                                                                                     >  2:122919/1‑149 (MQ=255)                                                             gtCGTGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACTCAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                    <  2:180542/149‑1 (MQ=255)                                                              gcGGGTAAGCCATTACTCTATCAGACACAGTGTTAAATCCTCTCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCGGGTTTAGCATCAATATAAATGTATGTTTTCCCGATTTCCCTTTTGAGGTTGATGTGc                                                                                   <  2:692637/148‑1 (MQ=255)                                                                gggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTACCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCACGATTTCCCTTTTGAGGTTGATGTGCAg                                                                                 >  2:607171/1‑149 (MQ=255)                                                                       ccATTACCCTATCCGACACACTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCAGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGa                                                                          <  2:1046404/149‑1 (MQ=255)                                                                        cATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAg                                                                         >  2:421856/1‑149 (MQ=255)                                                                          ttACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGtt                                                                       <  1:1128929/149‑1 (MQ=255)                                                                             cGCTATCCTACACAGTGTTAAATCCTCGCTTTTTTCCTTAGCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCGTGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGATGTTGATGTGCAGTTAACGAGTTTCa                                                                    <  2:928799/149‑1 (MQ=255)                                                                               cTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACt                                                                  <  2:780078/149‑1 (MQ=255)                                                                                tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTg                                                                 >  1:57732/1‑149 (MQ=255)                                                                                 aTCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTGTTGAGGTTGATGTGAAGTTAACGAGTTTCACTga                                                                >  1:1073440/1‑149 (MQ=255)                                                                                  tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                                                               <  2:599599/149‑1 (MQ=255)                                                                                    cGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatta                                                             >  1:564094/1‑149 (MQ=255)                                                                                     gACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACAGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                                            <  1:815175/149‑1 (MQ=255)                                                                                      acacAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacg                                                           <  1:455954/149‑1 (MQ=255)                                                                                        acaGTGTTAACTCCTCGCGTTTTTCCTTCCCCGCACGGAAATAAATTAGCTACACCGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGTTTGATGTGCAGTTAACGAGTTTCACTgattacgga                                                         <  2:101518/149‑1 (MQ=255)                                                                                         caGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggat                                                        <  1:485955/149‑1 (MQ=255)                                                                                          aGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                       >  1:294207/1‑149 (MQ=255)                                                                                           gtgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatta                                                      <  1:1071637/149‑1 (MQ=255)                                                                                            tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTAACTgattacggattac                                                     >  1:1195550/1‑149 (MQ=255)                                                                                              ttAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                   >  1:360998/1‑149 (MQ=255)                                                                                                aaaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACATCCTGTGGCTGGTTTATCATCAATATAAATGTATTTATTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgc                                                 <  1:536262/149‑1 (MQ=255)                                                                                                 aaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg                                                >  2:925711/1‑149 (MQ=255)                                                                                                      tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCTTCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                           >  2:611345/1‑149 (MQ=255)                                                                                                      tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                           >  1:362398/1‑149 (MQ=255)                                                                                                         cTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTa                                        <  1:489093/149‑1 (MQ=255)                                                                                                                ccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCTATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCg                                 <  1:712815/149‑1 (MQ=255)                                                                                                                ccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCg                                 >  1:1197906/1‑149 (MQ=255)                                                                                                                 cttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGAGTACGGATTACGTGCGCTGATCTACATGGCGt                                >  1:554759/1‑149 (MQ=255)                                                                                                                     cccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGGTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCAtt                            <  2:1096060/149‑1 (MQ=255)                                                                                                                      ccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTGCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGAGTACGGATTACGTGCGCTGATCGACATGGCGTCATTg                           >  2:271170/1‑149 (MQ=255)                                                                                                                      ccGAACTGAAATAAATTAGCGACACAGCCAGTGGCTGGATTATCATCAATATAAAAGTATTTTTTCCCGATATCCCTTTTCAGGTTGATGTCCAGTAAACGACATTCACTGATTACGGATTACTAGCGATGAACTACATGGCGTCAATg                           >  2:333334/1‑149 (MQ=255)                                                                                                                            tGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGTGGTTGATGTGCTGTTAACGAGTTTCAGTGATTACGGTTTACGTGCGCTGATGTACATGGCGTAATTGCAAGaa                     >  2:1218780/1‑149 (MQ=255)                                                                                                                            tGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGaa                     >  2:496214/1‑149 (MQ=255)                                                                                                                                ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc                 <  1:607171/149‑1 (MQ=255)                                                                                                                                ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc                 >  2:38068/1‑149 (MQ=255)                                                                                                                                ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc                 >  1:626487/1‑149 (MQ=255)                                                                                                                                 taaatTAGCGACCCAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCTCTTTCCCTTTTGAGTTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACTTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg                <  2:731332/149‑1 (MQ=255)                                                                                                                                 taaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg                <  2:293527/149‑1 (MQ=255)                                                                                                                                     ttAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGTCGGATg            >  2:30817/1‑149 (MQ=255)                                                                                                                                     ttAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATg            >  1:751859/1‑149 (MQ=255)                                                                                                                                       aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTTATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc          <  2:1195550/149‑1 (MQ=255)                                                                                                                                       aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc          <  1:133279/149‑1 (MQ=255)                                                                                                                                        gCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAcc         >  2:935236/1‑149 (MQ=255)                                                                                                                                          gACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAg       >  1:215271/1‑149 (MQ=255)                                                                                                                                           acacAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGt      >  2:456755/1‑149 (MQ=255)                                                                                                                                           acacAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCAATGATTACGGATTACGTGAGCTGATCTACATGGCGTCATTGCCAGAAGGTCGGATGACAAGt      >  2:54231/1‑149 (MQ=255)                                                                                                                                            cacaGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTa     <  1:496214/149‑1 (MQ=255)                                                                                                                                               aGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACTGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAgtt  >  2:250077/1‑149 (MQ=255)                                                                                                                                               aGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAttt  >  1:498300/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  AATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTT  >  NC_000913/4405984‑4406274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑