BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,286,945	G→C	5.7%	intergenic (+419/+121)	narI → / ← rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,286,945	0	G	C	5.7%	354.4 / 11.5	117	intergenic (+419/+121)	narI/rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand): ref base G (44/65); new base C (3/4); total (48/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

AACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCT  >  NC_000913/1286799‑1287094

aaCCTTGTTCGTAGCTTCTCATCGTTACCCGCTTGGGCAGATTTTTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                                                                                                                                                    <  2:421883/150‑1 (MQ=14)

    ttGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAc                                                                                                                                                <  2:2630235/150‑1 (MQ=255)

    ttGGTCGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAc                                                                                                                                                <  2:2630126/150‑1 (MQ=255)

    ttGGTCGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGCAGGCAAc                                                                                                                                                <  2:1595661/150‑1 (MQ=255)

      ggTCGAAGCTTCTCATCGTTCCCCGCTTGGGCAGAAGATTTGATTGCGGATTCGTTTGAGAATTCGGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGCAGGCAACga                                                                                                                                              <  2:1626326/150‑2 (MQ=14)

         cGAAGCTTCTGATCCTTACCCGCTTGGGCAGATTATTTGATTGCGGATTAGTTTGAGATTTCGGGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCATTGCCTGCGGCAACGctct                                                                                                                                           <  2:62564/150‑1 (MQ=11)

         cGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATATGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCATTGCCTGCGGCAACGctct                                                                                                                                           <  2:62690/150‑1 (MQ=255)

             gcgtctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCg                                                                                                                                       <  2:2451381/147‑1 (MQ=255)

              cTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGc                                                                                                                                      >  2:387423/1‑150 (MQ=255)

                tctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCGTTCGGGTCGTTGCCTGCGGCACCGCTATCTCGCTg                                                                                                                                    >  2:2738383/1‑150 (MQ=255)

                tatcATCCTTTGCCGCTTGGGGAGTATATTCGTTTGCGTAGTAGTTTGAGTTTTCCTTTATTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTg                                                                                                                                    <  2:2879699/148‑1 (MQ=11)

                 ctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGTAAGGGATACACATCTCTACTCGATTCGCCCTTAGGGAAGTTGCATGCGGCAAAGCTCTCTAGCTGa                                                                                                                                   >  1:1993060/1‑150 (MQ=11)

                 ctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCTCGcggg                                                                                                                                   >  1:939577/1‑147 (MQ=1)

                    aTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGgcgc                                                                                                                                >  2:2777347/1‑150 (MQ=255)

                     tcgttCCCCGCTTGGTCAGAATATTTGATTGCGGATTCGTGTGTGTTTTCCGGGGCTTTTGCTAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                               <  2:2977097/147‑1 (MQ=14)

                     ccgttCCCCGCTCGGGCAGAATATTTGATTGCGGACTCGTGTGAGAAGTGCGGGTCTTATGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                               <  2:739049/147‑1 (MQ=14)

                        ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                            >  2:488080/1‑150 (MQ=255)

                        ttCCCCGCTTGGGCAGAATAGTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                            >  2:1035674/1‑150 (MQ=255)

                            ccGCTTTGGCATATTATTTGATTGCGGATTCGTTTGAGAATTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                        <  2:323838/150‑1 (MQ=255)

                                ttGGCCAGAATATTTGATTGCGGATTCGATTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAcc                                                                                                                    <  1:2725770/150‑1 (MQ=255)

                                   ggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTa                                                                                                                 <  1:1143847/150‑1 (MQ=255)

                                   ggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTa                                                                                                                 <  1:387423/150‑1 (MQ=255)

                                     cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                               >  1:203453/1‑150 (MQ=255)

                                     cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                               >  2:74595/1‑150 (MQ=255)

                                     cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGt                                                                                                               >  1:2827083/1‑150 (MQ=255)

                                     cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGt                                                                                                               >  1:1280814/1‑150 (MQ=255)

                                         atatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGCGCTTCGCCCTTCGGGTCGTTTCCTCCGGCAACGCTCTCTAGCTGACCCTCGAGCCGAACCGTTGTCGaa                                                                                                           >  2:983023/1‑150 (MQ=14)

                                          tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                          <  1:1353383/150‑1 (MQ=255)

                                          tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAg                                                                                                          >  2:616580/1‑150 (MQ=255)

                                              ttaTTTCGGATTCGTTTGAGTATTCGTGTTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                      <  2:690654/148‑1 (MQ=14)

                                                aTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCACCCCTCTCACGCAGACGCACGAGCCGCACCCTAGTCGAAGCAtctc                                                                                                    >  1:1812026/1‑150 (MQ=14)

                                                aTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCATCGGGTCGTTTCCGGCGGCAACCCACTCAAGCAGACGCTCGAGCCGCAACATAGTGGAAGcgtaaa                                                                                                    >  1:1369607/1‑145 (MQ=14)

                                                 ttGCTGATTCGTTTGAGAATTCCGGTTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                   <  2:2529109/150‑1 (MQ=255)

                                                 ttGCGGATTGGTTTGAGTATTCGGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                   <  1:2791746/150‑1 (MQ=255)

                                                 ttGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                   >  2:353352/1‑150 (MQ=255)

                                                  tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGTTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                  <  1:1183791/150‑1 (MQ=255)

                                                   gCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATc                                                                                                 >  2:204855/1‑150 (MQ=255)

                                                     ggATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGACGAACCATAGTAGAAGCTTCTCaaact                                                                                               >  2:1129414/1‑146 (MQ=14)

                                                       aTTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTCACGCTGCCGCTCGAGCCGAACCATAGTAGAAGCTTCTcatccatc                                                                                             >  2:611037/1‑150 (MQ=14)

                                                            tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCCGCAACGCTCACTCGCTGGCGCTCGAGCCGAACCATGGTCGAAGCTTCTCAGCCTGCCCCGc                                                                                        >  1:856462/1‑150 (MQ=11)

                                                            tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCCCTTCGCCCTTCGGGTCGTTTCCTGAGGCAAAGATCTCTCGCTGACGCACGATCCGAACCTTATTCGAAGCTTCTAATACATCCCCGc                                                                                        >  2:684830/1‑150 (MQ=14)

                                                             ttttgatttCCGGGGCTTTTGACAGTGTTGGTGGTGGGGTAAGGATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                       <  1:1838472/143‑1 (MQ=14)

                                                              tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                      <  2:966282/150‑1 (MQ=255)

                                                               gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCAGGCGCGCGAGCCGAACCGTGGTCGAATCATCTCAACCATCCCCGCaag                                                                                     >  1:2185254/1‑148 (MQ=11)

                                                                  aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAACGCACACACCCTGACGCACCACCCGAACCTTAGTCGAACCTTCTCAACCGTACCCCCAAGGGc                                                                                  >  2:2876899/1‑150 (MQ=14)

                                                                  aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCCGCAACGCACGCTCGCTTACCCACCACTCGGACCTTAGTACAAGCATCACAACCATACCCCCAAGGGc                                                                                  >  2:2877067/1‑150 (MQ=2)

                                                                             ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                       <  1:660983/150‑1 (MQ=38)

                                                                              tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGATGCCAGCGGCAACCCTCTCTCCCTGACGCTCGAGCCGAACCATAGTCGAAGCAACTCAACCAACCCCCCAACGGCAGAAAATTTGaa                                                                      >  2:2269287/1‑149 (MQ=11)

                                                                              tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTCCGCCCATCGCCCAACGGCTCGATGCCTTCCGCAACGCACGCACCCAGACGCCCCAACCGAACCTTACTCGAAGCTACACATCAAACCCCCCATGGGCACACTAATTGAt                                                                      >  2:2269309/1‑150 (MQ=2)

                                                                                  gaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCg                                                                  <  1:652249/149‑1 (MQ=39)

                                                                                  gaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCg                                                                  <  1:2521080/149‑1 (MQ=39)

                                                                                  aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCg                                                                  >  2:921930/1‑150 (MQ=39)

                                                                                   aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCgg                                                                 >  2:1991381/1‑150 (MQ=21)

                                                                                    aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGa                                                                <  2:203453/150‑1 (MQ=39)

                                                                                    aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGa                                                                <  2:1280814/150‑1 (MQ=39)

                                                                                    aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGa                                                                <  2:2827083/150‑1 (MQ=39)

                                                                                                  gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGtt                                                  <  1:74595/150‑1 (MQ=255)

                                                                                                  gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGtt                                                  <  1:286188/150‑1 (MQ=255)

                                                                                                  gggAAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCttccttaaa                                                  <  2:39241/150‑8 (MQ=255)

                                                                                                    gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                                <  1:2895801/150‑1 (MQ=255)

                                                                                                     caggaggaCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCGCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTGGATTGCGGATTCACTTGAGAGTTCAg                                               <  2:1804679/143‑1 (MQ=14)

                                                                                                     aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                               <  2:1408480/150‑1 (MQ=255)

                                                                                                     aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCACTTGAGAGTTCAg                                               <  2:2445377/150‑1 (MQ=255)

                                                                                                      aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                              <  1:2934863/150‑1 (MQ=255)

                                                                                                      aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                              <  1:921930/150‑1 (MQ=255)

                                                                                                         aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAACCGTCCCCGCAGGGGCAGAATATTTGATTGCGGATTCGGTGGGGAGTTCAGGGAc                                           >  2:2430836/1‑150 (MQ=11)

                                                                                                             cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                       <  1:204855/150‑1 (MQ=255)

                                                                                                               cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCTTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTGATTGCGGATTCGGGTGTGAGTTCAGGGGCTTTTGa                                     >  1:2581725/1‑150 (MQ=14)

                                                                                                                 gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                   <  1:1755570/150‑1 (MQ=255)

                                                                                                                  cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                  <  1:2447624/150‑1 (MQ=255)

                                                                                                                  cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                  <  1:381777/150‑1 (MQ=255)

                                                                                                                   gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                 <  2:1093348/150‑1 (MQ=255)

                                                                                                                   gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                 <  2:2804471/150‑1 (MQ=255)

                                                                                                                   gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                 <  1:2428161/150‑1 (MQ=255)

                                                                                                                   gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                 <  1:1844997/150‑1 (MQ=255)

                                                                                                                   gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                 <  2:669993/150‑1 (MQ=255)

                                                                                                                   gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                 <  1:1310088/150‑1 (MQ=255)

                                                                                                                     tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGA                               <  2:434012‑M1/150‑1 (MQ=255)

                                                                                                                     tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGA                               <  2:2771383‑M1/150‑1 (MQ=255)

                                                                                                                     tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGA                               <  1:473984‑M1/150‑1 (MQ=255)

                                                                                                                       cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGGGAGTTCAGGGACGTTTGAAAGTGATG                             >  2:954068‑M1/1‑150 (MQ=255)

                                                                                                                       cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATG                             <  2:924569‑M1/150‑1 (MQ=255)

                                                                                                                               gCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGG                     <  2:2882484‑M1/150‑1 (MQ=255)

                                                                                                                                cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATAGTTGATTGCGGATTCGCTTGTGCGGTCAGGGACGTTTGAAAGTGAGGGGGGGGGGG                    >  2:786186‑M1/1‑150 (MQ=255)

                                                                                                                                cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCACCCTGACGCTCGAGCCCAACCTTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGCAAATTTTATTTCGGATTCGCGTGGGAGTTCAGGGACTGTTGAAAGTGAGGGTGGTGGGG                    >  2:1177202‑M1/1‑150 (MQ=255)

                                                                                                                                cccTTCGGGCCGTTGCCCGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGG                    <  1:3059601‑M1/150‑1 (MQ=255)

                                                                                                                                  cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                  <  1:1695082‑M1/150‑1 (MQ=255)

                                                                                                                                  cTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCACGAGCCGAACCGTAGTCGAAGCGTCTCATCCGTACCCGCAAGGGCCGAATATTTCATTGCGGATTCGGTTGGGAGTTCAGGGACTTTTGAAAGTGATGGGGGGGGGGGa                  >  2:2935941/1‑150 (MQ=14)

                                                                                                                                    tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG                <  1:3107364‑M1/150‑1 (MQ=255)

                                                                                                                                    tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG                <  1:353352‑M1/150‑1 (MQ=255)

                                                                                                                                         tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa           <  2:1946636/150‑1 (MQ=255)

                                                                                                                                           gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt         <  1:2738383/150‑1 (MQ=255)

                                                                                                                                            ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTccac        >  2:3099761‑M1/1‑146 (MQ=255)

                                                                                                                                            ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc        >  2:3099799/1‑150 (MQ=255)

                                                                                                                                            ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc        >  2:565340/1‑150 (MQ=255)

                                                                                                                                            ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc        >  2:1335094/1‑150 (MQ=255)

                                                                                                                                            ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTGTGGAAAGTGATGGTGGTGGGGGAAGGATTACTc        >  2:366719/1‑150 (MQ=255)

                                                                                                                                            ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTACTGGGAGTGTAGAGAGTGATCGTGGTGGGGGAAGGATTACTc        >  2:3115643/1‑150 (MQ=17)

                                                                                                                                             tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa       <  1:1602239/150‑1 (MQ=255)

                                                                                                                                                cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCAACCATCCCCCCATGGGCCGAAAATTTGATTTCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTaaacacag    >  1:2262688/1‑143 (MQ=17)

AACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCT  >  NC_000913/1286799‑1287094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`