Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,552,079 C→A 7.3% intergenic (+88/+319) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,552,0790CA 7.3% 452.7 / 21.5 137intergenic (+88/+319)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (40/87);  new base A (3/7);  total (43/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 150 total aligned reads displayed.

GCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTA  >  NC_000913/1551933‑1552227
                                                                                                                                                  |                                                                                                                                                    
gcTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcggc                                                                                                                                                   <  1:465085/150‑1 (MQ=255)
gcTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcggc                                                                                                                                                   <  2:1303380/150‑1 (MQ=255)
gcTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcggc                                                                                                                                                   <  2:689007/150‑1 (MQ=255)
  tGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGt                                                                                                                                                 <  2:2766085/150‑1 (MQ=255)
   ggTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTg                                                                                                                                                <  1:23331/150‑1 (MQ=255)
   ggTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTg                                                                                                                                                <  1:22998/150‑1 (MQ=255)
    gTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGa                                                                                                                                               <  1:1197084/150‑1 (MQ=255)
    gTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATAAGGCGttc                                                                                                                                               <  1:1597489/150‑3 (MQ=255)
     tATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                              >  1:838732/1‑150 (MQ=255)
      aTCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                             <  2:2345354/150‑1 (MQ=255)
      aTCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                             <  2:353945/150‑1 (MQ=255)
      aTCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                             <  2:473378/150‑1 (MQ=255)
        cGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                           >  2:804670/1‑150 (MQ=255)
         gTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                          <  1:2998700/150‑1 (MQ=255)
         gTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                          <  2:508557/150‑1 (MQ=255)
         gTGAAATCGAGAACGCAGAAGCGAAAAAAGAGAGTGAAGAAGTGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                          <  2:2886889/150‑1 (MQ=255)
          tGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTCAAAGGCGCCATTTTCATATTGTAGACAACGTCGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                         >  1:457126/1‑150 (MQ=255)
             aaTCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                      >  1:2175322/1‑150 (MQ=255)
               tCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                    <  2:3062903/150‑1 (MQ=37)
               tCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                    <  2:2611826/150‑1 (MQ=37)
                cGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                   <  2:2027597/150‑1 (MQ=255)
                 gagaAGGCAGAAGCGAAAACAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                  <  2:2727613/150‑1 (MQ=25)
                 gagaAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                  <  2:839567/150‑1 (MQ=255)
                 gagaAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                  <  2:2727574/150‑1 (MQ=25)
                 gagaAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAAGCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                  <  1:1480909/150‑1 (MQ=255)
                  agaAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                 <  1:642536/150‑1 (MQ=255)
                   gaAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                                                                <  1:535310/150‑1 (MQ=255)
                   gaAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                                                                <  1:2603990/150‑1 (MQ=255)
                    aaGGCAGAAGCGAAATAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                               >  1:827081/1‑150 (MQ=255)
                    aaGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                               >  1:1146275/1‑150 (MQ=255)
                      ggCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGa                                                                                                                             >  2:1538104/1‑150 (MQ=255)
                           aaGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAcc                                                                                                                        <  2:1132232/150‑1 (MQ=255)
                           aaGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAcc                                                                                                                        <  1:325441/150‑1 (MQ=255)
                                     agagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATcc                                                                                                              >  2:1591796/1‑150 (MQ=255)
                                     agagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATcc                                                                                                              >  1:2592684/1‑150 (MQ=255)
                                      gagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCa                                                                                                             <  1:1285259/150‑1 (MQ=255)
                                      gagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCa                                                                                                             <  2:1856123/150‑1 (MQ=255)
                                      gagagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCa                                                                                                             <  1:2829758/150‑1 (MQ=255)
                                       agagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCaa                                                                                                            <  1:2382794/150‑1 (MQ=255)
                                        gagTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                                                                                           <  2:1666681/150‑1 (MQ=255)
                                         agTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATa                                                                                                          >  1:335368/1‑150 (MQ=255)
                                              agaagGGATATAATCTCTTTTGAACTTTACGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTg                                                                                                     <  1:1404693/150‑1 (MQ=255)
                                              agaagGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTg                                                                                                     <  1:2346883/150‑1 (MQ=255)
                                                    gATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGa                                                                                               >  1:562502/1‑150 (MQ=255)
                                                    gATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGa                                                                                               <  1:1449051/150‑1 (MQ=255)
                                                     atatAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGaa                                                                                              <  2:2369236/150‑1 (MQ=255)
                                                      tataATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAAc                                                                                             >  1:1471471/1‑150 (MQ=255)
                                                          aTCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAGAGATTGCAGTGAACgtgt                                                                                         >  2:149583/1‑150 (MQ=255)
                                                             tctTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAgg                                                                                      >  2:3067729/1‑150 (MQ=255)
                                                               ttttGAACTTTTAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGcc                                                                                    <  2:2612369/150‑1 (MQ=255)
                                                               ttttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGcc                                                                                    >  2:2197524/1‑150 (MQ=255)
                                                                tttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCt                                                                                   <  1:2634012/150‑1 (MQ=255)
                                                                tttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCt                                                                                   <  2:2975554/150‑1 (MQ=255)
                                                                 ttGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTg                                                                                  <  1:1717732/150‑1 (MQ=255)
                                                                       tttAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGc                                                                            >  1:2879952/1‑150 (MQ=40)
                                                                       tatcaGCTTAAAATGGCGCTGTAAAAGGCGACATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGc                                                                            <  2:1902557/146‑1 (MQ=0)
                                                                        ttAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCg                                                                           <  2:686643/150‑1 (MQ=39)
                                                                         tAAGCTTAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGt                                                                          <  1:1658595/150‑1 (MQ=25)
                                                                          aaGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTa                                                                         >  1:2812368/1‑150 (MQ=38)
                                                                           aGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg                                                                        >  2:2783476/1‑150 (MQ=37)
                                                                              tGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcgc                                                                     <  2:1624847/150‑1 (MQ=34)
                                                                               gAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCa                                                                    <  1:2398359/150‑1 (MQ=33)
                                                                                aaaaTGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGa                                                                                               >  2:2437321/1‑122 (MQ=33)
                                                                                 aaaTGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCaac                                                                  <  1:3067486/150‑3 (MQ=17)
                                                                                 aaaTGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAGGAAAACCCTTCAAATCCAATAGATTGCAGTGACCGGGTAGGCCTGATAAGCGTCGCGCATc                                                                  >  1:2663904/1‑150 (MQ=255)
                                                                                    tGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                               <  2:1820577/150‑1 (MQ=32)
                                                                                          tGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGt                                                         >  2:2543904/1‑150 (MQ=32)
                                                                                            tAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTg                                                       <  1:962100/150‑1 (MQ=32)
                                                                                             aaaaGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGc                                                      <  1:1338924/150‑1 (MQ=32)
                                                                                               caGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGt                                                    <  2:2069863/149‑1 (MQ=17)
                                                                                               aaGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGt                                                    <  1:1249510/150‑1 (MQ=32)
                                                                                               aaGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGt                                                    <  1:2138906/150‑1 (MQ=32)
                                                                                                aGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGtt                                                   <  2:2683029/150‑1 (MQ=32)
                                                                                                aGGCGCCATTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGttt                                                  <  1:2879062/150‑1 (MQ=17)
                                                                                                   cgcCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGt                                                <  2:1543531/150‑1 (MQ=32)
                                                                                                   cgcCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGAGAAGCGTAGCGCATCAGGCAATGTTGCGTTTGt                                                <  2:1078316/150‑1 (MQ=17)
                                                                                                    gcCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGtc                                               >  1:2465091/1‑150 (MQ=31)
                                                                                                    gcCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGAAGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGtc                                               <  1:1001980/150‑1 (MQ=14)
                                                                                                             atatTGTAGACCCCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAGTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTc                                      <  1:918242/150‑1 (MQ=9)
                                                                                                             atatTGTAGACACCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTc                                      <  1:917847/150‑1 (MQ=14)
                                                                                                                ttGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaaa                                   >  2:1971469/1‑150 (MQ=33)
                                                                                                                ttGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTGCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATACGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaaa                                   >  2:2976178/1‑150 (MQ=18)
                                                                                                                    aGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGGGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgc                               >  1:1010534/1‑150 (MQ=21)
                                                                                                                      aCAACGTAGGCTTTGTTCATGCCGGGTGCGGCGTGTACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGTACGTGTAGGCCAGATAAGCGTAGCGCATCAGGCCATGTTGCGTGTGTCATCAGTTTGAAATGgcgc                             >  1:2916615/1‑150 (MQ=11)
                                                                                                                      aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCTAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgcgc                             <  2:3021634/150‑1 (MQ=21)
                                                                                                                         aCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTTAAATGGCGCTGt                          >  1:1782818/1‑150 (MQ=14)
                                                                                                                                cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGc                   <  2:1719861/150‑1 (MQ=255)
                                                                                                                                  ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGt                 <  2:85446/150‑1 (MQ=255)
                                                                                                                                         tGCCGGATGCGGCGTGAACGCCATATCCCGCATGAAAACCCTTCAAATACAAAAGAATGCAGTGAACGTGTAGGCCAGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAAATGCGCTGTAAAACGCGTCAtatta          >  1:2900702/1‑149 (MQ=14)
                                                                                                                                          gCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCa         <  1:2543904/150‑1 (MQ=255)
                                                                                                                                           ccGGATAAGGCGTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGTTTGCAGTGAACGTGTAGGCCTGTTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTATAAGGGGCCAttttgtt        >  2:2766592/1‑147 (MQ=11)
                                                                                                                                           ccGGATAAGGCGTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGGAGCGCATCAGGCAAGGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTACAAGGCGGCATTTTAat        >  2:711976/1‑150 (MQ=14)
                                                                                                                                                tGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCTATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGGTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGt   <  1:2073043/150‑1 (MQ=255)
                                                                                                                                                tGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGt   <  2:1321466/150‑1 (MQ=255)
                                                                                                                                                 gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa  <  1:1894389/150‑1 (MQ=255)
                                                                                                                                                 gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa  <  1:689164/150‑1 (MQ=255)
                                                                                                                                                 gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa  <  1:115989/150‑1 (MQ=255)
                                                                                                                                                 gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa  <  2:898350/150‑1 (MQ=255)
                                                                                                                                                 gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGAGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa  <  1:1894309/150‑1 (MQ=255)
                                                                                                                                                 gcggcgTGAACGCCTGATCCGGCATGAAAACCCTTCACATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa  <  2:898349/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCTGGTATCGTGAAATCGAGAAGGCAGAAGCGAAAAAAGAGAGTGAAGAAGGGATATAATCTCTTTTGAACTTTAAGCTGAAAATGGCGCTGTAAAAGGCGCCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTA  >  NC_000913/1551933‑1552227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: