BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,919,279	A→G	7.6%	V590V (GTA→GTG)	yebT →	MCE domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,919,279	0	A	G	7.6%	538.4 / 26.7	170	V590V (GTA→GTG)	yebT	MCE domain protein
Reads supporting (aligned to +/- strand): ref base A (87/70); new base G (6/7); total (93/77)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 172 total aligned reads displayed.

GGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCA  >  NC_000913/1919130‑1919428

ggTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTa                                                                                                                                                       <  1:2671708/150‑1 (MQ=255)

 gTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTAc                                                                                                                                                      >  1:516371/1‑150 (MQ=255)

 gTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTAc                                                                                                                                                      >  1:2374251/1‑150 (MQ=255)

    aaGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAgg                                                                                                                                                   <  1:2222683/150‑1 (MQ=255)

       tGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCAt                                                                                                                                                >  1:2335374/1‑150 (MQ=255)

          ttACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATccc                                                                                                                                             <  2:1419998/150‑1 (MQ=255)

           tACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATcccc                                                                                                                                            <  2:1165570/150‑1 (MQ=255)

           tACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATcccc                                                                                                                                            >  1:782285/1‑150 (MQ=255)

            aCCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCg                                                                                                                                           <  2:1898474/150‑1 (MQ=255)

              cGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGct                                                                                                                                         <  1:2724745/150‑1 (MQ=255)

                 gCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCtctc                                                                                                                                      <  1:1082011/150‑1 (MQ=255)

                 gCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCtctc                                                                                                                                      >  1:764553/1‑150 (MQ=255)

                 gCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCtctc                                                                                                                                      <  1:583489/150‑1 (MQ=255)

                 gCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCtctc                                                                                                                                      <  1:1679841/150‑1 (MQ=255)

                     ccGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCaga                                                                                                                                  <  2:1081765/150‑1 (MQ=255)

                     ccGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCaga                                                                                                                                  <  2:666591/150‑1 (MQ=255)

                       gcgAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGc                                                                                                                                >  1:807069/1‑150 (MQ=255)

                       gcgAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGGTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGc                                                                                                                                >  2:2224441/1‑150 (MQ=255)

                        cgAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCa                                                                                                                               >  2:1707498/1‑150 (MQ=255)

                             tAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTGCAGGCATCCCCGCTCTCCAGAGCATTaaa                                                                                                                          <  1:2777612/150‑1 (MQ=255)

                             tAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTaaa                                                                                                                          >  1:1075973/1‑150 (MQ=255)

                                cgcgTTTGATATCGATCTGCATATTAAGGCGGTGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAggg                                                                                                                       <  1:474957/150‑1 (MQ=255)

                                cgcgTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAggg                                                                                                                       <  1:2045832/150‑1 (MQ=255)

                                 gcgTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGa                                                                                                                      <  1:2945018/150‑1 (MQ=255)

                                 gcgTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGa                                                                                                                      <  2:199193/150‑1 (MQ=255)

                                 gcgTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGa                                                                                                                      <  1:2397470/150‑1 (MQ=255)

                                 gcgTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGa                                                                                                                      <  1:1142834/150‑1 (MQ=255)

                                   gTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGc                                                                                                                    >  2:3103316/1‑150 (MQ=255)

                                      tGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCAt                                                                                                                 >  1:2025474/1‑150 (MQ=255)

                                      tGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCAt                                                                                                                 >  1:453989/1‑150 (MQ=255)

                                              aTCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCg                                                                                                         >  2:1802092/1‑150 (MQ=255)

                                              aTCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCg                                                                                                         >  2:288952/1‑150 (MQ=255)

                                              aTCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCg                                                                                                         >  2:2318582/1‑150 (MQ=255)

                                                cTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGAc                                                                                                       >  1:305750/1‑150 (MQ=255)

                                                cTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGAc                                                                                                       >  1:305753/1‑150 (MQ=255)

                                                         aaGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGc                                                                                              >  2:2819234/1‑150 (MQ=255)

                                                         aaGCCGGAGTATCGAAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGc                                                                                              <  1:2608939/150‑1 (MQ=255)

                                                           gCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCgg                                                                                            >  2:2636734/1‑150 (MQ=255)

                                                           gCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCgg                                                                                            >  2:2338918/1‑150 (MQ=255)

                                                            ccGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGt                                                                                           <  2:1411677/150‑1 (MQ=255)

                                                            ccGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGt                                                                                           <  1:692413/150‑1 (MQ=255)

                                                              ggATTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGc                                                                                         <  2:540283/150‑1 (MQ=255)

                                                              ggAGTATCGCATCCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTATAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGc                                                                                         <  1:1641821/150‑1 (MQ=255)

                                                              ggAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGc                                                                                         >  1:2080767/1‑150 (MQ=255)

                                                              ggAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGc                                                                                         <  1:1641835/150‑1 (MQ=255)

                                                              ggAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGc                                                                                         <  2:2111236/150‑1 (MQ=255)

                                                               gAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGcc                                                                                        >  1:2454303/1‑150 (MQ=255)

                                                                aGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCa                                                                                       <  1:78283/150‑1 (MQ=255)

                                                                    tCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTGCAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAgcgc                                                                                   >  2:1443646/1‑150 (MQ=255)

                                                                    tCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAgcgc                                                                                   <  2:1584494/150‑1 (MQ=255)

                                                                      gCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCa                                                                                 >  1:1771696/1‑150 (MQ=255)

                                                                       cAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTGCAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAg                                                                                >  1:2579474/1‑150 (MQ=255)

                                                                       cAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTGCAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAg                                                                                >  2:1670136/1‑150 (MQ=255)

                                                                           cTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTGCAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAg                                                                            <  1:1917206/150‑1 (MQ=255)

                                                                            ttCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGc                                                                           >  2:2194373/1‑150 (MQ=255)

                                                                                 aCCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTaaa                                                                      >  2:42787/1‑150 (MQ=255)

                                                                                   cAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAgg                                                                    >  2:768115/1‑150 (MQ=255)

                                                                                   cAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAgg                                                                    >  2:1864319/1‑150 (MQ=255)

                                                                                     gCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCg                                                                  >  2:1751651/1‑150 (MQ=255)

                                                                                           gCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGc                                                            >  2:1336761/1‑150 (MQ=255)

                                                                                           gCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGc                                                            >  1:1810724/1‑150 (MQ=255)

                                                                                           gCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGc                                                            >  2:2612224/1‑150 (MQ=255)

                                                                                           gCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGc                                                            >  1:1190962/1‑150 (MQ=255)

                                                                                           gCGTGTTCGGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGc                                                            <  2:120528/150‑1 (MQ=255)

                                                                                                 tCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTc                                                      <  2:764262/150‑1 (MQ=255)

                                                                                                   tGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTg                                                    <  2:2451052/150‑1 (MQ=255)

                                                                                                   tGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTg                                                    >  2:2074729/1‑150 (MQ=255)

                                                                                                     ggCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCCTCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTa                                                  <  2:2385042/150‑1 (MQ=255)

                                                                                                     ggCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTa                                                  <  2:807069/150‑1 (MQ=255)

                                                                                                     ggCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTa                                                  <  2:1349967/150‑1 (MQ=255)

                                                                                                       cAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATg                                                <  1:552649/150‑1 (MQ=255)

                                                                                                       cAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATg                                                >  1:1276176/1‑150 (MQ=255)

                                                                                                        aGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGc                                               >  2:1589249/1‑150 (MQ=255)

                                                                                                          aaGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCtt                                             <  2:1617609/150‑1 (MQ=255)

                                                                                                          aaGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCtt                                             <  2:1617587/150‑1 (MQ=255)

                                                                                                           aGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTc                                            <  2:1310451/150‑1 (MQ=255)

                                                                                                               ggggCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTGCAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGaa                                        >  2:2839748/1‑150 (MQ=255)

                                                                                                                  gCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACa                                     <  1:2831619/150‑1 (MQ=255)

                                                                                                                  gCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACa                                     >  2:2783495/1‑150 (MQ=255)

                                                                                                                  gCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACa                                     >  1:2233393/1‑150 (MQ=255)

                                                                                                                  gCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACa                                     <  2:2425904/150‑1 (MQ=255)

                                                                                                                    gAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAagcagc                                   >  2:1520900/1‑150 (MQ=255)

                                                                                                                    gAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAgt                                   >  1:586962/1‑149 (MQ=255)

                                                                                                                    gAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGc                                   >  2:1308619/1‑150 (MQ=255)

                                                                                                                    gAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGc                                   >  2:1896124/1‑150 (MQ=255)

                                                                                                                               tGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCgg                        >  2:2130482/1‑150 (MQ=255)

                                                                                                                               tGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCgg                        >  1:2222784/1‑150 (MQ=255)

                                                                                                                                      tAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCgg                 >  1:526250/1‑150 (MQ=255)

                                                                                                                                       aGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCggg                <  1:1589249/150‑1 (MQ=255)

                                                                                                                                        gTGGTCTGACCGCACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGc               >  2:2508013/1‑150 (MQ=255)

                                                                                                                                         tGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCa              >  2:1494605/1‑150 (MQ=255)

                                                                                                                                         tGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCa              <  1:2636734/150‑1 (MQ=255)

                                                                                                                                         tGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCa              <  1:892217/150‑1 (MQ=255)

                                                                                                                                               gACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTAc        >  2:948850/1‑150 (MQ=255)

                                                                                                                                                aCCGTGCAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACg       <  1:2653264/150‑1 (MQ=255)

                                                                                                                                                aCCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTATCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGAGCGGTTGGCGGGCAGATTACg       >  1:2875615/1‑150 (MQ=255)

                                                                                                                                                aCCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACg       >  2:2982355/1‑150 (MQ=255)

                                                                                                                                                aCCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACg       <  1:2885159/150‑1 (MQ=255)

                                                                                                                                                  cGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCt     >  1:1203256/1‑150 (MQ=255)

                                                                                                                                                    tACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTc   >  1:1888119/1‑150 (MQ=255)

GGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTATTCTGTATGCTTCCGAAACAGCGGCCCGTGCGGTTGGCGGGCAGATTACGCTTCA  >  NC_000913/1919130‑1919428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`