Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 10.0% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA10.0% 348.5 / 2.7 137intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (68/48);  new base A (13/7);  total (82/55)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  NC_000913/2177086‑2177382
                                                                                                                                                    |                                                                                                                                                    
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                     <  1:684686/150‑1 (MQ=255)
   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCaaaa                                                                                                                                                  >  1:1895269/1‑150 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAat                                                                                                                                                 >  1:2819851/1‑150 (MQ=255)
    cAGCGGTGTCTACCACCACTTGCATCGGTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCGGGGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCAGTCGTTTTGTTTCGGTTTAaaaaa                                                                                                                                                 >  1:1295958/1‑149 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCaaaaaa                                                                                                                                                >  2:2892265/1‑148 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                               <  1:2616641/150‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAatat                                                                                                                                               >  1:314095/1‑150 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                              <  1:1520854/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                              <  1:3059265/150‑1 (MQ=255)
            tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCAGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                         >  1:640580/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACTGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                       >  1:886218/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaaa                                                                                                                                       >  2:1474179/1‑149 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGAGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATATTCATATCCTGTCGTTTTTTTTCGATTTCAAAAAAAAAAGAAat                                                                                                                                       >  1:1958572/1‑150 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                   <  2:349946/150‑1 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                   <  1:2493005/150‑1 (MQ=255)
                     ccTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                <  2:1131/149‑1 (MQ=255)
                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATCTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                <  2:851283/150‑1 (MQ=255)
                        tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCACATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                             <  2:2712962/150‑1 (MQ=255)
                         gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGAAATTATtttttttt                                                                                                                            >  1:1978371/1‑150 (MQ=255)
                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGAAAATATTTTTTTTTa                                                                                                                           >  2:844309/1‑150 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATAAAATGAAATTATTTTTTTTTaat                                                                                                                         >  1:1160638/1‑149 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                         >  2:2568757/1‑150 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                         >  2:473231/1‑150 (MQ=255)
                                ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                     <  2:1959290/150‑1 (MQ=255)
                                ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                     <  2:1959426/150‑1 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                    <  1:343245/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                   <  2:1746708/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                   <  2:1769315/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                   <  2:1887067/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                   <  1:991633/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                   <  2:2984047/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                   <  2:310947/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                            <  1:2430785/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATTAAATTATTTTTTTTTAAATATCGAGATAACGAt                                                                                                          >  1:128416/1‑150 (MQ=255)
                                                 tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATAACaaa                                                                                                    >  2:2220583/1‑150 (MQ=255)
                                                 tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAGAACGATcacaca                                                                                                    >  2:1872872/1‑148 (MQ=255)
                                                 tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAAATATTTGTTTTTAAAAATCGAGATAACGATCACaaa                                                                                                    >  2:154075/1‑150 (MQ=255)
                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                  <  2:1557345/150‑1 (MQ=255)
                                                    aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                 <  1:729142/150‑1 (MQ=255)
                                                       ccagAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                              <  2:640580/147‑1 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTGAAAATAAAATTAAAATATTTGTTTTTTAATATAGAGATAAAGATAACaaaaaaaaca                                                                                             >  1:2532886/1‑145 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                             >  2:2341797/1‑150 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTGGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGGCGGGTGTGTTCGATTGCCAAATATCATGGAAGTATTTGTTTGTAAATATCGCGCTAACGGTCACAAAAACGGCa                                                                                             >  2:3021673/1‑150 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTGGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGGTTTCGCTTTCAAAATATAATGACATTAGTTGTTTTTAAATATCGAGAGACCGATCCCACACACgccc                                                                                             >  2:2528482/1‑147 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATCGAGATAAAGATCACAAAAAAGACaa                                                                                            >  2:2819055/1‑150 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACaa                                                                                            >  2:2507515/1‑150 (MQ=255)
                                                              cGCATACCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGa                                                                                       <  2:299818/150‑1 (MQ=255)
                                                              cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGa                                                                                       >  2:197970/1‑150 (MQ=255)
                                                                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTAAATTATTTTTTTTTAAAAATAGAGATAAAGATCACAAAAAAGACAAAATGata                                                                                     >  1:1501316/1‑148 (MQ=255)
                                                                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAAAATGAAAATATTTGTTTTTAAATATAGAGATAACGATAACAAAAACGACAATATGaaa                                                                                     >  1:1639791/1‑150 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAat                                                                                   >  2:692640/1‑150 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAat                                                                                   >  2:807024/1‑150 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGTAATTAATTTTTTTTAAATATCGAGATAAAGATAACAAAAACGAAAATAAGAAAat                                                                                   >  2:807019/1‑150 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                              <  2:1093724/150‑1 (MQ=255)
                                                                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAATTATTTTTTTTTAAATATAGATATAAAGAACACAAAAAAGACAATATTAAAATTATTAGAGGAGTGaaa                                                                   >  1:743092/1‑150 (MQ=255)
                                                                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTAAAAATATAATGAAAATATTTTTTTTTTAATATAGAGTTAAAGATAACAAAAAAGAAAAAATGAAAATTATTAGAGGGGTGtaa                                                                   >  1:477781/1‑150 (MQ=255)
                                                                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATAACAAAAAAGACAAAAAGAAAATTATTAGAGGAGTGAAAg                                                                  >  2:349018/1‑150 (MQ=255)
                                                                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTCAAAATATAATGTAATAATTTTTTTTTTAATATAGAGATAAAGATCACAAAAAAGAAAAAAATTAAATTATTAGAGgtgtttaat                                                                  >  1:681690/1‑145 (MQ=255)
                                                                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTATCATATCCTGTCGTTTGTTTTCGAATTCAAAATAAAATGAAATTAATTGTTTTTAAATAACGAGATAAACATCACAAAAACGACAATATGAAAATTATTAGAGGAGGGAAAg                                                                  >  2:1217270/1‑150 (MQ=255)
                                                                                      gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGAGTGAAAGGCa                                                               >  2:3060664/1‑150 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATAATTTTTTTTTAAAATAGAGATAAAGATAAAAAAAAAGAAAAAAAGAAAAATATTAGAGGAGTGAAAGGCaaaa                                                            >  1:2045495/1‑150 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAATAAAGAAAATAATTTTTTTTAAATATCCAGATAACCAACACAAAAACCACAAAATGAAAAATATTCGCGGAGTGAAACGCcaaa                                                            >  2:2423865/1‑150 (MQ=255)
                                                                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTAAAAAAAAAAATAAATAATTATTTTTTAAAAAACGACATCACCATCAAAAAAACCACAAAAAGAAAAAAAATCGAGGAGTGCAAGGCacaaa                                                           >  1:3105830/1‑150 (MQ=255)
                                                                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCaaaaaa                                                          >  2:761374/1‑150 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCACTTACTTTTTAATATCCTTTCTTTTTTTTTATATTACAAAATATAATTAAATTATTTTTTTTTAAATATCTAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                        <  1:360020/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCACGTACTTTTTCTTATCCTGTCTTTTTTTTTCTATTTAAAAATATATTGAATTTATTTTTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                        <  1:359835/150‑1 (MQ=255)
                                                                                               cTGGTTTGTCGATCCCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                      <  2:2158365/150‑1 (MQ=255)
                                                                                                        ctaTACCACTTACATTTTCATATCCTGTCTTTTGTTTTCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                             <  2:1927007/148‑1 (MQ=255)
                                                                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATTATGAAATTATTTGTTTTTAAATATGGAGATTACGTTGACAAAAACGTCAATATTAAAATTATTGGATGTGTGTAATGCAAAAAAAAGGGCTGCCGATAgggt                                        >  2:287077/1‑149 (MQ=255)
                                                                                                              cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                       >  2:1292854/1‑150 (MQ=255)
                                                                                                                cttaattttaCATATCCGGTCGTTTTTTTTCTATTAAAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                     <  1:2217871/144‑1 (MQ=255)
                                                                                                                 gTACTTTTCAATACCCTGTGGTTTGTTTGCTATTTCAAAATAAATTTAATTTTTTTTTTTTTAAATATCGATAAACCGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGcc                                    <  1:1397856/150‑1 (MQ=255)
                                                                                                                 gTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                    <  2:2658011/150‑1 (MQ=255)
                                                                                                                  tACATTTTCATATCCTGTCGTTTGTTTTCGAGTTAAAAATATAATGAAATTATTTTTTTTTTAATATCGAGATAACGATCACAAAAACGACAAAATGAAAAATATTGGAGGCGTGAAAGGCAAAAAAACGGCCTCCCCAGAGGGAAGCCg                                   >  2:2918387/1‑150 (MQ=255)
                                                                                                                    atttttCTTATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGAGATAACGAACACAAACACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                 <  1:320177/148‑1 (MQ=255)
                                                                                                                       tttCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCACCCGATAGGGAAGCCGTAGCa                              >  2:2648741/1‑150 (MQ=255)
                                                                                                                       tttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCACCCGATAGGGAAGCCGTAGCa                              >  2:704889/1‑150 (MQ=255)
                                                                                                                         taatatCGTGTCTTTTGTTTTCGTTTTAAAATAATAATCAATTTTTTTTTTTTTAAATATCTATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                            <  1:891890/148‑1 (MQ=255)
                                                                                                                            tatCCTGTCTTTTGTTTTCTTTTTAAAAATATAATTAATTTATTTTTTTTTAAATATCGATATAACTATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                         <  1:2104943/150‑1 (MQ=255)
                                                                                                                                     gtttgtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                <  1:2334520/150‑1 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt               >  1:723609/1‑150 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt               >  1:1994585/1‑150 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt               >  1:2965732/1‑150 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt               >  1:1837763/1‑150 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt               >  1:2921435/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg           >  1:2349151/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg           >  2:2088075/1‑150 (MQ=255)
                                                                                                                                          ttatCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg           >  2:1041656/4‑150 (MQ=255)
                                                                                                                                            ttctttttCAAATTATAATTAAATTTTTTGTTTTTAAATATCTTTATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc         <  1:1990919/145‑1 (MQ=255)
                                                                                                                                              cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAATCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAATCGGCCTCCCGATAGGGAAGCGGTAGCTAAGTGCGCGTGTTTTTATGCCgg       >  2:556747/1‑150 (MQ=255)
                                                                                                                                              cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg       >  2:338314/1‑150 (MQ=255)
                                                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATCGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      >  2:983624/1‑150 (MQ=255)
                                                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      >  2:1146745/1‑150 (MQ=255)
                                                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      >  2:924831/1‑150 (MQ=255)
                                                                                                                                                aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt     >  1:509737/1‑150 (MQ=255)
                                                                                                                                                 tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg    >  1:447618/1‑150 (MQ=255)
                                                                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc   >  1:2724765/1‑150 (MQ=255)
                                                                                                                                                   tCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg  <  1:2646617/150‑1 (MQ=255)
                                                                                                                                                   acAAAATATATGTAATTTATTTTTTTTTAAATTTCTATATAACTATCACAAACACGACAATATGAATATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGTTGCg  <  1:2646614/149‑1 (MQ=255)
                                                                                                                                                   acAAAATATAATCAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg  <  1:2883648/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  NC_000913/2177086‑2177382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: