Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,618 A→T 16.2% intergenic (+73/‑326) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6180AT16.2% 335.0 / 7.9 126intergenic (+73/‑326)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base A (83/15);  new base T (2/19);  total (86/40)
Fisher's exact test for biased strand distribution p-value = 5.83e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTC  >  NC_000913/3239469‑3239766
                                                                                                                                                     |                                                                                                                                                    
tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                                      <  2:2054768/150‑1 (MQ=255)
 gtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGagag                                                                                                                                                     <  1:2868493/150‑1 (MQ=255)
  tttGGCATTCTGGTGATGACGTTTATTTTCAACGCCTTGGTGAATTATCGGCATGTTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                                    <  2:2985995/150‑1 (MQ=255)
  tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                                    >  2:789166/1‑150 (MQ=255)
  tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                                    >  2:1672074/1‑150 (MQ=255)
  tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                                    >  2:2581117/1‑150 (MQ=255)
  tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                                    <  1:2498648/150‑1 (MQ=255)
  tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                                    <  1:3059850/150‑1 (MQ=255)
    tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                                  >  2:1015277/1‑150 (MQ=255)
               tgatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                                       >  2:1368021/1‑150 (MQ=255)
               tgatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                                       <  2:2102418/150‑1 (MQ=255)
               tgatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                                       >  1:1634850/1‑150 (MQ=255)
               tgatgaCGTTTATAATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                                       >  1:52911/1‑150 (MQ=255)
                      gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                >  2:1469620/1‑150 (MQ=255)
                      gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                >  2:599510/1‑150 (MQ=255)
                      gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                >  1:1911660/1‑150 (MQ=255)
                      gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                >  1:1847052/1‑150 (MQ=255)
                      gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCACTCTCCCTTCCAGGGAGTGGGTCTGGGTGAGGGTAAtttt                                                                                                                                >  1:2562760/1‑150 (MQ=255)
                        ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGTATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGTGAGGGTCGGGGTGAGGGTAATTTTTc                                                                                                                              <  2:752480/150‑1 (MQ=255)
                        ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCCCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTc                                                                                                                              <  2:752515/150‑1 (MQ=255)
                         tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                             >  1:1560413/1‑150 (MQ=255)
                         tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                             >  1:1560290/1‑150 (MQ=255)
                            tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                          >  2:244279/1‑150 (MQ=255)
                            tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                          >  2:1524305/1‑150 (MQ=255)
                            tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                          >  1:2826646/1‑150 (MQ=255)
                            tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                          >  2:1197576/1‑150 (MQ=255)
                              tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                                                                                        >  1:2486521/1‑150 (MQ=255)
                              tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                                                                                        >  1:2486347/1‑150 (MQ=255)
                                aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                                      >  1:1705973/1‑150 (MQ=255)
                                aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                                      >  1:130111/1‑150 (MQ=255)
                                  cGCCTGGTTTAATTTTCGGCTTGATAAGCTGGGGGTTGGATATTTTTTAATCTGCCTACGCCGTGTACCCTGTCATTAACTTGCGCACCGTTTTCTCCCGCTCCCGTCCCGGGTGAGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGc                                                                                                                    <  2:1579399/150‑1 (MQ=255)
                                     cTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGATGGTAATTTTTCGCACCGATGCTgg                                                                                                                 >  2:2147243/1‑150 (MQ=255)
                                     cTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTgg                                                                                                                 >  2:2079111/1‑150 (MQ=255)
                                         gTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                                                                                             >  1:2258388/1‑150 (MQ=255)
                                             aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcc                                                                                                         >  2:1378941/1‑150 (MQ=255)
                                             aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGAGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcc                                                                                                         >  2:381640/1‑150 (MQ=255)
                                              ttATCGGCATGATACGCAGTGGGGTGGATAATTTTTAATCTGCCTACGCGGTGTACCCTTTCATTAACATGCGCACCTTTTTCTCCCTCTCCCTTCCCGGGAGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                                                                                                        <  2:2064782/150‑1 (MQ=255)
                                                    gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                                  >  1:205611/1‑150 (MQ=255)
                                                       tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                                                                                               >  1:971670/1‑150 (MQ=255)
                                                       tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                                                                                               >  1:190354/1‑150 (MQ=255)
                                                        gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                              <  2:2861469/150‑1 (MQ=255)
                                                          tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                            >  1:1080256/1‑150 (MQ=255)
                                                              cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAACCctct                                                                                        >  2:685717/1‑150 (MQ=255)
                                                               tgcGGGTTGGATAATTTTTAATCGGCCTACGCGGTGTACCCTGTCATTACCTTGAGCACCGTTTTCTCCCGCGCCCTTCCCGGGAGTGGGTGGGGTTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                       <  2:1704701/149‑1 (MQ=25)
                                                                gcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTAACCTGTCATTAACATGAGCACAGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGGGAGGGTAATTGTTCGCACCGATGGTGGCCTGGTCACCTCACCCTAAACCTATcc                                                                                      >  1:125813/1‑150 (MQ=255)
                                                                 gggggTGGATATTTTTTAAGGGGCCTACGCGGTTTACCCGGTCTTTAACTTGAGCACGGTTTTCCCCCTCGCCCTCCCCGGGTGTGGGTGGGGGTTTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTccc                                                                                     <  2:2698212/149‑1 (MQ=16)
                                                                  ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTcccc                                                                                    >  1:2857295/1‑150 (MQ=255)
                                                                   gggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                   >  2:2851961/1‑150 (MQ=255)
                                                                     gtgtATAATTTTTAATCTGCATATGCGGTGTACCCTGTATTTAACATGAGCACCGTTTTCTCCGTCTCCCTTCCCGGGTGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                 <  2:2520035/150‑1 (MQ=25)
                                                                        gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAcag                                                                              >  1:2231816/1‑148 (MQ=255)
                                                                          tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                            >  1:1450292/1‑150 (MQ=255)
                                                                          tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                            >  2:7108/1‑150 (MQ=255)
                                                                          tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAACgggg                                                                            >  1:1450297/1‑150 (MQ=255)
                                                                           attttttAATCTGCATATGCGTTTTACCTTTTAATTAACTTTTCCACGGTTTTCCCCCTCCCCCTTCCTGGTTTTGGGTGGGGTTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                           <  2:92948/148‑1 (MQ=12)
                                                                                ttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                                      >  2:1907321/1‑150 (MQ=255)
                                                                                     cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTg                                                                 >  1:1899611/1‑150 (MQ=255)
                                                                                     cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCACTCCCCAAACGGGGCGAGGGGGCTg                                                                 >  1:1078068/1‑150 (MQ=255)
                                                                                     cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTAGCACCGATGCTGGCCTGTTACCCAAACCCAAAACCACTCCCCAAACGGGGCGAGGGGACTg                                                                 >  1:1362540/1‑150 (MQ=255)
                                                                                     cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCGTCCCGGGAGCGGGTCGGGGTGTGGGTAATTTTTCGCACCGCTGCTGGCCGGGTCCCCTCGCCCTAACCCGCTCCCCAAACGGGGGGAGGGGGCTg                                                                 >  2:2513477/1‑150 (MQ=255)
                                                                                          tAAGCCGTGTACCCTGTCATTAAAATGAGCACCGTTTTCTCCCTCTCCCTTCCTGGTTTTGGGTGGGGGTTAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                            <  2:729931/150‑1 (MQ=37)
                                                                                            cgCCGTGGCCCCGGCCATTACCCGGCGCCCCGTTTGCCCCCCCGCCCGGCCCGGGCGCGGGGCGGGGTGCGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGt                                                          <  2:2593123/149‑1 (MQ=12)
                                                                                                gtgtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtt                                                      >  2:633485/1‑150 (MQ=255)
                                                                                                   tACCCTGTCTTTAACAGGCCCACGGTTTACTCCCGCCCCGTTCCGGGGATGGGGTGGGGGGGTGGGAAATTTTTCGCACCAATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttttt                                                   <  2:859307/150‑1 (MQ=21)
                                                                                                   tACCCTGTCATTACCATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttttt                                                   <  2:3090850/150‑1 (MQ=255)
                                                                                                    aCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                  <  1:1297305/150‑1 (MQ=255)
                                                                                                    aCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                  <  2:3068834/150‑1 (MQ=255)
                                                                                                    aCCCTGTATTTAACTTGAGCCCCGTTTTCCCCCGCTCCCTTCCCGGGAGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                  <  2:1531389/150‑1 (MQ=21)
                                                                                                     cccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTg                                                 >  2:1186491/1‑150 (MQ=255)
                                                                                                      ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                                >  1:1592607/1‑150 (MQ=255)
                                                                                                       cTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                               >  2:2203175/1‑150 (MQ=255)
                                                                                                       cTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                               >  1:2942804/1‑150 (MQ=255)
                                                                                                        tGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                              >  2:2170754/1‑150 (MQ=255)
                                                                                                           cATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGTTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttg                                           <  2:1521575/150‑1 (MQ=255)
                                                                                                            aTTACCTTGAGCACCGTTTTCGCCCTCTCCCTGCCTGGGTGTGGGTGGGGGTGCGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                          <  1:2074224/150‑1 (MQ=25)
                                                                                                             ttACCATGAGCACGGTTTGCCCCCTCCCCGTCCCGGGGTGTGGGTGGGGGTTGGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                         <  2:2643839/150‑1 (MQ=21)
                                                                                                               aaCATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                                       <  2:3091668/150‑1 (MQ=255)
                                                                                                               aaCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                                       >  1:2158057/1‑150 (MQ=255)
                                                                                                               aaCATGAGCACCGTTTTCTCCCTCTCCCTACCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                                       <  1:2311006/150‑1 (MQ=255)
                                                                                                                aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                                      >  1:2858037/1‑150 (MQ=255)
                                                                                                                aCATGAGCACCGTTTTCTCCCTCCCCCTTCCTGGGTGTGGGTGGGGTTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                                      <  1:1801218/150‑1 (MQ=37)
                                                                                                                 caggAGCACCTTTTTCCCCCTCCCCCTCCCAGGGTGTGGGGGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatca                                     <  1:597281/147‑1 (MQ=21)
                                                                                                                   tGAGCACCGTTTTCTCCCTCTCCCTTCCTGGGTGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                                   <  1:1511345/150‑1 (MQ=255)
                                                                                                                   tGAGCACCGTTTTCTCCATATCCCTTCCTGGGATTGGGTCGGTGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGTGCTAGGGGACTGAACGAGTCCTTTTTTGATGTTGTCATCAGt                                   <  1:1055522/150‑1 (MQ=37)
                                                                                                                       cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                               >  2:1509372/1‑150 (MQ=255)
                                                                                                                         ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCGCTCCCCAAACGGGGCGAGGGGACGGACCGAGTCCGTTTGTGCGGGTGTCATCAGTCTGGac                             >  2:2811234/1‑149 (MQ=25)
                                                                                                                              tcccccctctccctCCCTGGGTGGGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGc                        <  1:2370211/146‑1 (MQ=25)
                                                                                                                                 tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg                     >  1:920189/1‑150 (MQ=255)
                                                                                                                                  ccctccccctccCGGGGCGTGGTGGGGGGGGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                    <  1:2650201/150‑1 (MQ=37)
                                                                                                                                  ccccccccctCCCGGGGTGTGGGTGGGGGTCGGGCCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                    <  1:2650216/144‑1 (MQ=37)
                                                                                                                                    ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg                  >  1:2725768/1‑150 (MQ=255)
                                                                                                                                    ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg                  >  2:1242174/1‑150 (MQ=255)
                                                                                                                                    ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg                  >  2:149793/1‑150 (MQ=255)
                                                                                                                                      ctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGc                >  2:110534/1‑150 (MQ=255)
                                                                                                                                       tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt               >  1:2455136/1‑150 (MQ=255)
                                                                                                                                       tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt               >  2:534540/1‑150 (MQ=255)
                                                                                                                                        ccctTCCTGGGCGTGGGGGGGGTGGCGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCtt              <  1:1652284/150‑1 (MQ=37)
                                                                                                                                          ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa            >  1:1019178/1‑150 (MQ=255)
                                                                                                                                           ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGGGGGGACTGACCCAGTCCTTTTTTTATGTTGTCATCAGTCTGGAAGCCGCACGGTGGCTTTAt           >  1:541478/1‑150 (MQ=255)
                                                                                                                                           ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt           >  1:961494/1‑150 (MQ=255)
                                                                                                                                                    gagGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCAATCCCCAAACCGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCAACAGTCTGGAATCCGCACGTTGGCTTTATTTTTATGTc  >  1:2408490/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTC  >  NC_000913/3239469‑3239766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: