| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 702,338 | (T)7→8 | coding (414/1149 nt) | nagA ← | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 702,331 | 1 | . | T | 96.5% | 185.8 / 0.9 | 58 | coding (421/1149 nt) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (25/31); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCC‑T‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCA > NC_000913/702192‑702465 | gCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGcc > 1:1559248/1‑140 (MQ=255) tGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCC‑t‑tttt > 1:760984/1‑140 (MQ=255) tgatgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGAtt < 1:979367/140‑1 (MQ=255) tgatgaCTTCCGCAGGAACCATAACCGGTGCCAGGGTCACTTTGGTACTGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGCCTTACGCACAAAATTCGGATTATGGGTGCC‑TATTTTTGACCAGAtt < 2:365633/140‑1 (MQ=255) aaCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTcc < 2:967658/140‑1 (MQ=255) aaCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTcc < 1:1590039/140‑1 (MQ=255) aaCCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTcc < 1:1589674/140‑1 (MQ=255) tCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCa > 1:1192245/1‑140 (MQ=255) cGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGa > 1:738661/1‑140 (MQ=255) tttGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTc > 2:1195951/1‑140 (MQ=255) aaTGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGt < 1:1530175/140‑1 (MQ=255) tGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGttt < 2:260356/140‑1 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGAtt < 2:187832/90‑1 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGAtt > 1:187832/1‑90 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGAtt > 1:187869/1‑90 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGAtt > 1:188170/1‑90 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGAtt < 2:188170/90‑1 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGAtt < 2:187869/90‑1 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTg > 2:250746/1‑140 (MQ=255) aCGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCC‑T‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGc > 2:348610/1‑140 (MQ=255) gTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGcc < 1:1056339/140‑1 (MQ=255) gTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGcc < 1:1141680/140‑1 (MQ=255) ggCGTTTTCACACAGGAAATCTACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACATAACGCCTGATTCGGATGTTTTGCCAgg > 1:1727609/1‑140 (MQ=255) ggCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAgg > 1:1327442/1‑140 (MQ=255) ggCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAgg > 1:1327147/1‑140 (MQ=255) ttttCACACAGGAAATCGAACAGCGCGGCATCAGGAGTACGCACAAAATTGGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTACCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACt < 1:892425/140‑1 (MQ=255) ttCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcg > 2:1279089/1‑140 (MQ=255) caGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATa > 2:1048142/1‑140 (MQ=255) caGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATa > 2:1047936/1‑140 (MQ=255) aaTCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAAcgcg < 2:657779/140‑1 (MQ=255) aaTCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAAcgcg < 2:655476/140‑1 (MQ=255) aaTCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAAcgcg < 2:655804/140‑1 (MQ=255) tCGACCAGCGCGGCATCTGGCTTTCGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGTCCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCa < 2:414299/140‑1 (MQ=255) tCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCa < 1:189081/140‑1 (MQ=255) gACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACg < 1:282914/140‑1 (MQ=255) aCCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGc < 1:412714/140‑1 (MQ=255) aCCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGc < 2:1086159/140‑1 (MQ=255) ccAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGcc > 1:290494/1‑140 (MQ=255) cgcgGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGt < 1:1481191/140‑1 (MQ=255) cATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcat < 1:74823/140‑1 (MQ=255) cATCAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcat < 1:250746/140‑1 (MQ=255) cAGGCTTACGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAg > 2:103103/1‑140 (MQ=255) aCGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCg > 1:1538584/1‑140 (MQ=255) aCGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCg > 2:1689821/1‑140 (MQ=255) aCGCACAAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCg > 1:1677627/1‑140 (MQ=255) caAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggt < 1:201423/140‑1 (MQ=255) caAAATTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggt < 1:611929/140‑1 (MQ=255) aaaaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtg > 2:1169020/1‑140 (MQ=255) aaaaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtg > 1:1224056/1‑140 (MQ=255) aaaaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtg > 1:973718/1‑140 (MQ=255) aaaaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtg > 1:1455561/1‑140 (MQ=255) aaaaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtg > 1:874101/1‑140 (MQ=255) aaaaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtg > 1:720767/1‑140 (MQ=255) aaaaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtggtg > 1:720632/1‑140 (MQ=255) aaTTCGGATTATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTggtggt < 2:554611/140‑1 (MQ=255) ttATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCg < 2:1559248/140‑1 (MQ=255) ttATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCg < 1:708104/140‑1 (MQ=255) ttATGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCg < 2:856281/140‑1 (MQ=255) aTGGGTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTc < 1:610725/140‑1 (MQ=255) gTGCCTT‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCa < 1:1179429/140‑1 (MQ=255) | GCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGCC‑T‑TTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCA > NC_000913/702192‑702465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |