BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,406,129	T→C	intergenic (+144/‑61)	purA → / → nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,406,129	0	T	C	99.1%	420.8 / ‑4.3	108	intergenic (+144/‑61)	purA/nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (77/30); total (78/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 108 total aligned reads displayed.

CCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGG  >  NC_000913/4405996‑4406258

ccTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCTAACTGAAATAAATTAGCGACACAGCCTGTGGc                                                                                                                             >  1:257955/1‑140 (MQ=255)

   ggCAGATATTTTGCCTGCCGGGCTAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTgg                                                                                                                          >  2:868534/1‑140 (MQ=255)

   ggCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACCCAGCCTGTGGCTgg                                                                                                                          >  2:1227211/1‑140 (MQ=255)

      aGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGttt                                                                                                                       <  1:991073/140‑1 (MQ=255)

           ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcat                                                                                                                  <  2:320680/140‑1 (MQ=255)

           ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcat                                                                                                                  <  2:320613/140‑1 (MQ=255)

           ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcat                                                                                                                  <  1:1570325/140‑1 (MQ=255)

                  tgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAAtata                                                                                                           >  2:303350/1‑140 (MQ=255)

                   gccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaa                                                                                                          >  2:416182/1‑140 (MQ=255)

                    ccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTAATCATCAATATaaa                                                                                                         >  1:1024549/1‑140 (MQ=255)

                         cGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAt                                                                                                    >  1:706669/1‑140 (MQ=255)

                                  tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCg                                                                                           >  2:259824/1‑140 (MQ=255)

                                  tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCg                                                                                           >  1:1606391/1‑140 (MQ=255)

                                  tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCg                                                                                           >  1:1606228/1‑140 (MQ=255)

                                      aCATTGCTGTGTCGGGTATGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                                                       <  2:603310/140‑1 (MQ=255)

                                      aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                                                       <  2:1291729/140‑1 (MQ=255)

                                      aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                                                       <  2:600977/140‑1 (MQ=255)

                                        aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTcc                                                                                     >  1:1424058/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:1270006/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:1668940/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:1246374/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:1269220/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:344834/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  2:803099/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:879451/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:1004526/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCGGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                   >  1:523608/1‑140 (MQ=255)

                                          tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCATTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATATTTTCCCGATTTCACt                                                                                   >  1:1228109/1‑140 (MQ=255)

                                            cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCttt                                                                                 <  2:1532085/140‑1 (MQ=255)

                                            cTGTGTCGGGTAAGCCAATACGCAATCCGACACAGTGTTAAATACTAGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGAAACATCCAGTGGCTGGTTTATCATAAATATAAATGTATTTTTTCACGATTTACCttt                                                                                 >  2:293122/1‑140 (MQ=255)

                                                    ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGTACTGAAATAAATTAGCGACACAGCCTGGGGCTGGTTTATCATCAATATCAATGTATTGTTTCCCGAAATCCCGTTAGAGGTTg                                                                         >  2:1502162/1‑140 (MQ=255)

                                                    ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTg                                                                         >  1:316820/1‑140 (MQ=255)

                                                    ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTg                                                                         >  1:316817/1‑140 (MQ=255)

                                                      tAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                                                       >  1:143903/1‑140 (MQ=255)

                                                      tAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                                                       >  1:141514/1‑140 (MQ=255)

                                                         gCCATTACGCTATCCGACACAGTGTTAACTCCTCGCTTTTTTACTACCCCGAACTGTTATAAATTAGCGACAGAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                    <  2:827158/140‑1 (MQ=255)

                                                         gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                                                       <  2:451766/121‑1 (MQ=255)

                                                         gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                                                       >  1:1774142/1‑121 (MQ=255)

                                                         gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                    <  1:1615880/140‑1 (MQ=255)

                                                         gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                    <  1:1321659/140‑1 (MQ=255)

                                                         gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTACCCGAttt                                                                                       >  1:451766/1‑121 (MQ=255)

                                                         cccATTACGCAATCAGTCAATGTTCTAAATCCACGCTTTTTTACTGCACCGAAATGAAATAAATTCGCGACACAGCCTGTTGCTGGTTTAACATCAATATAAATGTATTTTTTCCAGATTTCCCTTTTGAGGTTGAtgtg                                                                    <  1:1751425/139‑1 (MQ=255)

                                                              tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                               >  2:1576999/1‑140 (MQ=255)

                                                              tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                               >  2:1289471/1‑140 (MQ=255)

                                                              tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                               >  1:500811/1‑140 (MQ=255)

                                                              tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                               >  2:576502/1‑140 (MQ=255)

                                                                   tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGa                                                          >  2:845740/1‑140 (MQ=255)

                                                                     tacGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCAACAATATAAATTTATTTTTTCCCGATTTCCCTTTTGAGGTGGATGTGCAGTAAACGAGt                                                        >  2:401696/3‑140 (MQ=255)

                                                                     tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAATTAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCGTATTGAGGTTGATGTGCAGTTAACGAGt                                                        >  2:401706/1‑140 (MQ=255)

                                                                     tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGt                                                        >  1:596719/1‑140 (MQ=255)

                                                                     tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGt                                                        >  1:767583/1‑140 (MQ=255)

                                                                     tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGt                                                        >  1:430188/1‑140 (MQ=255)

                                                                     tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGt                                                        >  1:1690568/1‑140 (MQ=255)

                                                                     tCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGt                                                        >  1:1690516/1‑140 (MQ=255)

                                                                         acacAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCa                                                    <  2:706669/140‑1 (MQ=255)

                                                                             aGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTga                                                >  2:321344/1‑140 (MQ=255)

                                                                              gtgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGTCACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat                                               <  2:1153692/140‑1 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGTGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  1:102764/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  2:1502858/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  2:1143390/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              <  1:1313764/140‑1 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  1:139752/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              <  1:286464/140‑1 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              <  1:286532/140‑1 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  2:1151775/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  1:441376/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTGTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  2:82333/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTGGTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              >  2:1596732/1‑140 (MQ=255)

                                                                               tgtTAAATCCTCGCTTTTTTCCGTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatt                                              <  1:293677/140‑1 (MQ=255)

                                                                                 ttAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                            >  1:1755873/1‑140 (MQ=255)

                                                                                 ttAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                            >  1:703725/1‑140 (MQ=255)

                                                                                    aaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacgga                                         >  1:104785/1‑140 (MQ=255)

                                                                                     aTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGTTTTATCATCAAAATAAATGTATTTTTTCCCGATTTACCTTTTGAGGTTGAAGTGAAGATAACGAGTTTAACTgattacggat                                        >  2:1411422/1‑140 (MQ=255)

                                                                                     aTCCTCGCTTTTTTCCTTCCCCGAACTGAAAAAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAATTGTATTTTTTCCCGAGTACCCGGTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggat                                        >  2:257676/1‑140 (MQ=255)

                                                                                       ccTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatta                                      >  2:318347/1‑140 (MQ=255)

                                                                                       ccTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatta                                      >  2:1562154/1‑140 (MQ=255)

                                                                                       ccTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGATTATCATCAATATAAATGTATTTTATCCCGATTACCCATAAGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggataa                                      >  2:1344413/1‑138 (MQ=255)

                                                                                       ccTCGCTTTTTTACTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCATTATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGATTCAATgattacggatat                                      >  2:1471303/1‑138 (MQ=255)

                                                                                        cTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac                                     <  1:1225454/140‑1 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGACATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTGACGAGTTTCACTgattacggattacg                                    >  1:755479/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  1:1070226/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCGCGATTTCCGTTTTGAGGTTGATGTGCATTTAACGAGTTTCACTgattacggattacg                                    >  2:1700015/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  2:1104688/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  2:1101722/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  1:1366368/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  2:591983/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  1:968845/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  2:452088/1‑140 (MQ=255)

                                                                                         tCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                    >  2:1108066/1‑140 (MQ=255)

                                                                                             ttttttCCTTCCCCGAACTGAAATAATTTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg                                <  2:122896/140‑1 (MQ=255)

                                                                                             ttttttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg                                <  1:1741323/140‑1 (MQ=255)

                                                                                             ttttttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg                                <  1:1034913/140‑1 (MQ=255)

                                                                                             ttttttACTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcg                                <  1:1616788/140‑1 (MQ=255)

                                                                                                ttctctaccccGAACTGAAAAAAATTATCGACACAGCCAGTGTCTGGTTTATCATCAATATAAAAGTATTTTTTCAAGATATCCCTTTAGAGGTAGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTg                             <  2:1729726/133‑1 (MQ=255)

                                                                                                             aCTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGt                <  2:631462/140‑1 (MQ=255)

                                                                                                                 aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCAtt            >  2:1350325/1‑140 (MQ=255)

                                                                                                                 aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCAtt            <  2:826713/140‑1 (MQ=255)

                                                                                                                 aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAAAATTAAATTATTTTTTCCCGAATTACCTATTTACGTTGTTTTGAATTTAAAGAGTATCAAATTTTACGGATTAAGTTCGCTTATCTTCATTGCGTAAtt            >  2:143652/1‑140 (MQ=255)

                                                                                                                   ataaatTAGCGACACATCCTGTGGCTGGATTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTATTAGGAAGATGAGCAGTAAACGAGTTTCACTGAATACGGATTACGTGCGATGATCTACATGGAGTCATTGc          >  1:1394844/1‑140 (MQ=255)

                                                                                                                           gCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAggg  >  1:168095/1‑140 (MQ=255)

CCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGG  >  NC_000913/4405996‑4406258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`