Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	494,987	G→T	G195W (GGG→TGG)	recR →	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	494,987	0	G	T	96.4%	250.4 / 1.4	84	G195W (GGG→TGG)	recR	gap repair protein
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (41/39);  total (44/40)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGA  >  NC_000913/494850‑495123                                                                                                                                          |                                                                                                                                         aaGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCtgg                                                                                                                                        <  1:305281/140‑1 (MQ=255) aaGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCtgg                                                                                                                                        <  1:969689/140‑1 (MQ=255) aaGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCtgg                                                                                                                                        >  1:681546/1‑140 (MQ=255)  aGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGc                                                                                                                                       <  1:389157/140‑1 (MQ=255)   gttGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAATCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCg                                                                                                                                      >  1:758231/3‑140 (MQ=255)      gAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCa                                                                                                                                   >  1:1705278/1‑140 (MQ=255)        aGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATa                                                                                                                                 >  2:84994/1‑140 (MQ=255)        aGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATa                                                                                                                                 >  2:250229/1‑140 (MQ=255)             ccGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGAtt                                                                                                                            <  2:1080410/140‑1 (MQ=255)             ccGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGAtt                                                                                                                            <  2:1619599/140‑1 (MQ=255)             ccGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGAtt                                                                                                                            <  2:1080396/140‑1 (MQ=255)             ccGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGAtt                                                                                                                            <  2:774710/140‑1 (MQ=255)                  aaCTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGttt                                                                                                                       <  2:327261/140‑1 (MQ=255)                  aaCTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGttt                                                                                                                       <  2:327306/140‑1 (MQ=255)                    cTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGttttt                                                                                                                     >  2:930118/1‑140 (MQ=255)                          tGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCaa                                                                                                               >  1:1280278/1‑140 (MQ=255)                          tGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGAGTCGTTTTTATGCaa                                                                                                               >  1:1280262/1‑140 (MQ=255)                           gCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCaaa                                                                                                              <  1:682700/140‑1 (MQ=255)                            ccGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAAc                                                                                                             <  1:678301/140‑1 (MQ=255)                               aGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgag                                                                                                          >  1:795821/1‑140 (MQ=255)                               aGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgag                                                                                                          >  1:1048876/1‑140 (MQ=255)                               aGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgag                                                                                                          >  1:410227/1‑140 (MQ=255)                               aGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgag                                                                                                          >  1:199618/1‑140 (MQ=255)                                gCTTTGCGCGCATTATGACGTGGACGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGTCGGCAACACGTTGTCACACTCCCTTGCCTGGGGTCATAAGATTCGTTTTTAAGCAAACgaga                                                                                                         <  2:800113/140‑1 (MQ=255)                                gCTTTGCGCGCAATATGACGTGGACGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATATGATTCGTTTTTAAGCAAACgaga                                                                                                         <  2:800089/140‑1 (MQ=255)                                gCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgaga                                                                                                         <  2:295038/140‑1 (MQ=255)                                gCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgaga                                                                                                         <  2:586344/140‑1 (MQ=255)                                gCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgaga                                                                                                         <  2:542791/140‑1 (MQ=255)                                gCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgaga                                                                                                         <  1:1241004/140‑1 (MQ=255)                                gCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgaga                                                                                                         <  2:1376717/140‑1 (MQ=255)                                gCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACgaga                                                                                                         <  1:193282/140‑1 (MQ=255)                                 cTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCTATagagag                                                                                                        >  1:333414/1‑140 (MQ=255)                                 cTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGagag                                                                                                        >  1:575565/1‑140 (MQ=255)                                 cTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTGGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGagag                                                                                                        >  2:1543794/1‑140 (MQ=255)                                 cTTAGCGCCCAATAACACGTGGAAGCCAGCCGAATCGCTAAAGGCGTTCCGGTAGGCGGCGCGCTGGAAAAGGTCGACGCCACCACGTTGTCACACTCCCTTGCCTGCCGTCATAAGATTCGTTGTTAAGCAAACGagag                                                                                                        >  2:1054173/1‑140 (MQ=255)                                          cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCAc                                                                                               <  1:1154156/140‑1 (MQ=255)                                                  cGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCtctc                                                                                       >  1:1445092/1‑140 (MQ=255)                                                     ggAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGTGATCCTGCTCTCGCt                                                                                    >  1:1106826/1‑140 (MQ=255)                                                     ggAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCt                                                                                    >  1:1679806/1‑140 (MQ=255)                                                     ggAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCt                                                                                    >  1:723965/1‑140 (MQ=255)                                                     ggAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCt                                                                                    >  1:266764/1‑140 (MQ=255)                                                     ggAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGAAAACGAGAGCAGGATCACCTGCTCTCGCt                                                                                    >  1:1106887/1‑140 (MQ=255)                                                     ggAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCt                                                                                    >  1:1057325/1‑140 (MQ=255)                                                       aaGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTg                                                                                  >  1:1665425/1‑140 (MQ=255)                                                       aaGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGAGGCGAGCTGGAAATTGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTg                                                                                  >  2:1615994/1‑140 (MQ=255)                                                       aaGCCAGCCGAATCGCTAATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTg                                                                                  >  2:1673388/1‑140 (MQ=255)                                                        agccagccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCAGGCGTCATAAGATTCGTATTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCATGa                                                                                 <  1:1255040/140‑1 (MQ=255)                                                            agccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAatt                                                                             >  2:129076/1‑140 (MQ=255)                                                            agccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAatt                                                                             >  2:452935/1‑140 (MQ=255)                                                              ccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAattat                                                                           >  1:1589723/1‑140 (MQ=255)                                                               cGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAattatt                                                                          <  1:487214/140‑1 (MQ=255)                                                                    cGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTACCTTGCCTTGCGTAATAAGATTCGTTTTTAAGCATACGAGTGCAGGATCACCTGCTCTCGCTTGAAATTTTTCTccc                                                                     <  1:922870/140‑1 (MQ=255)                                                                            gCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTcccc                                                             <  2:652127/140‑1 (MQ=255)                                                                            gCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTcccc                                                             <  2:652139/140‑1 (MQ=255)                                                                               ttCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTATTTAAGCAAACGAGAGCAGGATCACCTGCTCTAGCTTGAAATTATTCTCCCTTGTCCCCaac                                                          >  2:437127/1‑138 (MQ=255)                                                                                  ccgTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACCTTGTCACACTCCCTTGCCTGGCGTCATAAGATTAGTTAATAAGCAAACGAGAGCAGGATCACCTGCTCACGCATGAAATTATTCTCCCTTGTCCCCAtctct                                                       <  1:1360795/138‑1 (MQ=255)                                                                                     ttGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTccc                                                    <  2:928999/140‑1 (MQ=255)                                                                                       ggcggcGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCcac                                                  >  1:1102868/1‑140 (MQ=255)                                                                                           gcgAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATcc                                              <  2:813763/140‑1 (MQ=255)                                                                                           gcgAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATcc                                              <  2:813768/140‑1 (MQ=255)                                                                                              aGCTGGGAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCTTTAGTTTCGTTTTTATGCAATCGAGAGCAGGATCACCTGCTCTCGCTTGTAATTATTCTCCCTTGTCCCCATCTCTCCCACATCgtgt                                           >  2:1546856/1‑140 (MQ=255)                                                                                              aGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGAGTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGt                                           >  2:537661/1‑140 (MQ=255)                                                                                                 tGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGtttt                                        <  2:533529/140‑1 (MQ=255)                                                                                                            gACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTaaaa                             <  2:201815/140‑1 (MQ=255)                                                                                                            gACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTaaaa                             <  2:202057/140‑1 (MQ=255)                                                                                                            gACGGCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGAAACTAAATTAAGCAAACGAGAGCAGGATCACCTGCTCTAGCTTGAAATTATTCTCCCTTGACCCCATCTCACCCACATCCTGTTTTGAACCTTaaaa                             <  2:1312835/140‑1 (MQ=255)                                                                                                             aCGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAAt                            >  1:38368/1‑140 (MQ=255)                                                                                                               ggCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATgg                          <  1:208330/140‑1 (MQ=255)                                                                                                                gCACCACGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGc                         <  2:795821/140‑1 (MQ=255)                                                                                                                    cacGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCatta                     <  1:1527620/140‑1 (MQ=255)                                                                                                                    cacGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCatta                     <  1:1112736/140‑1 (MQ=255)                                                                                                                    cacGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCatta                     >  2:618097/1‑140 (MQ=255)                                                                                                                     acGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAATCAAACGAGAGCAGGATCACCTGCTCTCGCTTGATATTATTCTCCCTTGTCCCCATCTCTCCCACATCCAGTTTTTACCCTTAAAATGGCattat                    <  2:1236095/140‑1 (MQ=255)                                                                                                                     acGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCattat                    <  2:1048876/140‑1 (MQ=255)                                                                                                                     acGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGATTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCAACTCTCCCACATCCTGTTTTTAACCATAAAATGGCattat                    <  2:1726246/140‑1 (MQ=255)                                                                                                                      cGTTGTCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCAGCTCTCGCATGAAATTATTATCCCTTGTCCCCATCTCTCCCACTTCCTGTTTTTAACCTTAAAATGGCattatt                   <  2:1595792/140‑1 (MQ=255)                                                                                                                           tCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGt              >  2:24280/1‑140 (MQ=255)                                                                                                                           tCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGt              >  2:23887/1‑140 (MQ=255)                                                                                                                           tCACACTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGt              >  2:1383968/1‑140 (MQ=255)                                                                                                                              cacTCCCTTGCCTGGCGTCATCAGATTCGTTTGTAAGCAAACGAGAGCAGGCTCACATGCTCTCGCTTTAAATTATTCTCCCTTGTCGCCATCACTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGa           >  2:704216/1‑140 (MQ=255)                                                                                                                              cacTCCCTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGa           >  2:934819/1‑140 (MQ=255)                                                                                                                                   ccTTGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTAc      >  2:566639/1‑140 (MQ=255)                                                                                                                                     ttGCCTGGCGTCATACGATTCGTTTTTTAGAAAACGAGAGCAGGATCACCTGCTCTCGCTGGAAATTATTCGCCCATGTCCCCATCTCTCCCTCATCCTGTTGGTTACCTTAAAATGGCTTGATTGAGGTGGACCTACAt    >  2:616777/1‑140 (MQ=255)                                                                                                                                     ttGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACAt    >  2:909804/1‑140 (MQ=255)                                                                                                                                     ttGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACAt    >  2:909872/1‑140 (MQ=255)                                                                                                                                     ttGCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACATTAAAATGGCATTTTTGAGGTAGACCTActt    >  1:1083274/1‑138 (MQ=255)                                                                                                                                       gCCTGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGa  <  1:566639/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AAGGTGAAGCTACCGCTAACTACATTGCCGAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGA  >  NC_000913/494850‑495123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑