Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,049,313 G→A S7L (TCG→TTG)  gfcB ← O‑antigen capsule production lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,049,3130GA96.3% 243.6 / 1.2 83S7L (TCG→TTG) gfcBO‑antigen capsule production lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (40/38);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TAGTGGAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCGTGTAG  >  NC_000913/1049179‑1049449
                                                                                                                                      |                                                                                                                                        
tAGTGGAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATaaa                                                                                                                                     >  2:525059/1‑140 (MQ=255)
tAGTGGAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATaaa                                                                                                                                     >  2:525057/1‑140 (MQ=255)
tAGTGGAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATaaa                                                                                                                                     >  2:1486598/1‑140 (MQ=255)
tAGTGGAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATaaa                                                                                                                                     >  1:1361175/1‑140 (MQ=255)
   tGGAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATa                                                                                                                                  >  1:1483587/1‑140 (MQ=255)
    ggAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCaataaaataa                                                                                                                                 <  1:805886/140‑1 (MQ=255)
    ggAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCaataaaataa                                                                                                                                 <  1:805813/140‑1 (MQ=255)
    ggAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCGATAAAATaa                                                                                                                                 <  2:1484507/140‑1 (MQ=255)
      aaTAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAaga                                                                                                                               >  2:1557272/1‑140 (MQ=255)
           ggCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCTTACATCCCGCAAGAAATAGTGCGAAAATCAGTAAAATAAGAGGgcg                                                                                                                          >  2:1541039/1‑140 (MQ=255)
           ggCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGgcg                                                                                                                          <  1:525057/140‑1 (MQ=255)
           ggCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGgcg                                                                                                                          <  1:525059/140‑1 (MQ=255)
           ggCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGgcg                                                                                                                          >  1:1521102/1‑140 (MQ=255)
           ggCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGgcg                                                                                                                          >  2:1113148/1‑140 (MQ=255)
            gCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGgcgc                                                                                                                         <  1:295951/140‑1 (MQ=255)
                cGCCTGGATCTGCTGATCCTCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATTTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAATATAAGAGGGCGCAcat                                                                                                                     >  2:1262111/1‑138 (MQ=255)
                cGCCTGGATCTGCTGATCCGCTACGGTGATTTCCTGATTCTCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCACGAAATAGTGCGAATATCAATAAAATAAGAGGGCGCACCt                                                                                                                     >  2:1264491/1‑140 (MQ=255)
                cGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCt                                                                                                                     >  2:1245717/1‑140 (MQ=255)
                    tGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTaa                                                                                                                 <  1:1299109/140‑1 (MQ=255)
                           gctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAATTCAATAAAATAAGAGGGCGCACCTTTAATCCTTGa                                                                                                          >  1:402473/1‑140 (MQ=255)
                           gctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGa                                                                                                          <  1:1365572/140‑1 (MQ=255)
                           gctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGa                                                                                                          >  2:1263760/1‑140 (MQ=255)
                           gctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGa                                                                                                          >  2:289979/1‑140 (MQ=255)
                           gctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGa                                                                                                          >  2:760662/1‑140 (MQ=255)
                            ctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGaa                                                                                                         >  1:1003978/1‑140 (MQ=255)
                            ctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGaa                                                                                                         >  2:335086/1‑140 (MQ=255)
                            ctgATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCATTGaa                                                                                                         >  2:1013913/1‑140 (MQ=255)
                                         gTGATAACCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCCTACATTGCTGGCTGTGCGTACAACCCGCAAGAAATAGTGCGAAAATCAAGAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTACTTaaaa                                                                                            <  2:247552/140‑1 (MQ=255)
                                         gTGATAACCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTaaaa                                                                                            >  2:642812/1‑140 (MQ=255)
                                           gATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATTCTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTaaaaaa                                                                                          <  2:1412007/140‑1 (MQ=255)
                                           gATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTaaaaaa                                                                                          <  2:1360167/140‑1 (MQ=255)
                                           gATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTaaaaaa                                                                                          <  2:1412670/140‑1 (MQ=255)
                                              atCCTGATTATCGAAAAGGCAGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATg                                                                                       <  2:1088135/140‑1 (MQ=255)
                                                ccTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCa                                                                                     <  2:342840/140‑1 (MQ=255)
                                                  tgcataccGAAAAGGCTGGCGCGAACAGTATCGCCCAAACTTGGCTGGCTGTGCGAACATCCCGCAAGAAATAGTGCGAAAAACCATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCaaa                                                                                   <  2:1726826/133‑1 (MQ=255)
                                                  tGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCaaa                                                                                   <  1:844660/140‑1 (MQ=255)
                                                  tGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCaaa                                                                                   <  2:345012/140‑1 (MQ=255)
                                                  tGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCaaa                                                                                   <  2:704634/140‑1 (MQ=255)
                                                  tGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCaaa                                                                                   <  2:1159471/140‑1 (MQ=255)
                                                  agATTATCTAAAAGTCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCaaa                                                                                   <  2:1701855/139‑1 (MQ=255)
                                                   gATTATCGTAAAGGCTGGAGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAATATATAGTGCGAAAATCAATAAAATAAGAGGGCGCTCCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAt                                                                                  <  2:729836/140‑1 (MQ=255)
                                                   gATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAt                                                                                  <  1:1188235/140‑1 (MQ=255)
                                                   gATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAt                                                                                  <  2:731326/140‑1 (MQ=255)
                                                   gATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAt                                                                                  <  2:731174/140‑1 (MQ=255)
                                                    aTTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGATAATGAATAAAATAAGTGGGCGCACCTTTAATCCTTGTAAGGTTAATTAAAAATTTGCAAAtc                                                                                 >  2:1424896/1‑140 (MQ=255)
                                                    aTTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAtc                                                                                 >  1:1431071/1‑140 (MQ=255)
                                                    aTTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAtc                                                                                 >  2:667907/1‑140 (MQ=255)
                                                    aTTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAtc                                                                                 >  2:942642/1‑140 (MQ=255)
                                                    aTTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAtc                                                                                 >  1:301608/1‑140 (MQ=255)
                                                    aTTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAAtc                                                                                 >  1:336729/1‑140 (MQ=255)
                                                         cGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTaa                                                                            >  2:743116/1‑140 (MQ=255)
                                                              aGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAACCCCAAAGCTCAAAAGccc                                                                       >  1:795808/1‑140 (MQ=255)
                                                                 cTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGt                                                                    <  2:162318/140‑1 (MQ=255)
                                                                  tGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAATGCCCGGTa                                                                   >  1:816426/1‑140 (MQ=255)
                                                                  tGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTa                                                                   >  1:1037416/1‑140 (MQ=255)
                                                                          aaTGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAATGGTTAATTAAAATAATGCAAATCTCTAAAGCCCGGTACGAATATc                                                           >  2:1603863/1‑140 (MQ=255)
                                                                              tATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCaa                                                       >  2:655824/1‑140 (MQ=255)
                                                                                   aCCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTACTCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCggg                                                  <  2:1658381/140‑1 (MQ=255)
                                                                                   aCCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCggg                                                  >  2:948807/1‑140 (MQ=255)
                                                                                   aCCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTATAAAGCCCGGTACGAATATCGCAACCggg                                                  <  2:1404812/140‑1 (MQ=255)
                                                                                     cATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTa                                                <  1:1662924/140‑1 (MQ=255)
                                                                                      aTACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTAt                                               >  1:1080188/1‑140 (MQ=255)
                                                                                        aCTTAGCTGGCTGTGCGTACAACCCTCAAGAAATAGTGCGAAAATCAATAAAAAAAGAGGGCGCACCTTTAATCCTTGAAAGGTTATTAAAAAATCTGCAAATCTGTAAAGCCCGGTACGAATCTCGCAACCGGGTATCa                                             >  1:1627397/1‑140 (MQ=255)
                                                                                         cTTTGCGGGCGGTGCGTACATCCCGCAAGAAAGAGTGCGAACACCAATAAAATAAGAGGGCGCACCTTTAATCCTTGACAGGTTAATTAAAAAAATGCCCATCTCTAAAGCCCGGTACGAATATAGCAAGCGGGTATCAt                                            <  2:71451/140‑1 (MQ=255)
                                                                                            tGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCACCCGGGTATCagata                                         >  2:257054/1‑136 (MQ=255)
                                                                                                 gctgTGCGTACATCCCGCAAGAAATAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGATAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACttt                                    >  1:866634/1‑140 (MQ=255)
                                                                                                         tACATCCCGCAAGAAATTGTGCGATAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCTAATCTCTAATGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGa                            <  2:957513/140‑1 (MQ=255)
                                                                                                         tACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGa                            <  1:504236/140‑1 (MQ=255)
                                                                                                         tACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGa                            <  1:1191343/140‑1 (MQ=255)
                                                                                                         tACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGa                            <  1:891209/140‑1 (MQ=255)
                                                                                                         tACATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGa                            <  1:891647/140‑1 (MQ=255)
                                                                                                           cATCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGAcg                          >  2:1349678/1‑140 (MQ=255)
                                                                                                             tCCCGCAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGAcgcg                        >  1:1254828/1‑140 (MQ=255)
                                                                                                                  cAAGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGAAACCGGGTATCATTTAACTTTAATTTCGACGCGTTaaa                   >  2:595541/1‑140 (MQ=255)
                                                                                                                   aaGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTTAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAg                  <  1:1245717/140‑1 (MQ=255)
                                                                                                                   aaGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAg                  <  1:308839/140‑1 (MQ=255)
                                                                                                                   aaGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAg                  <  1:1557272/140‑1 (MQ=255)
                                                                                                                   aaGAAATAGTGCGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAg                  <  2:331784/140‑1 (MQ=255)
                                                                                                                        aTAGTGCGAAAATCTATAATATAAGAGGGCGCACCTTTTATCCTTGAAAGGTTAATTATAAAAATGCAAATCTCTAATGCCCGGTACGAATATAGCAACCGGGTATCATTTAACTTTATTTTCGACGCGTTTAAGCAGTa             <  2:1711169/140‑1 (MQ=255)
                                                                                                                        aTAGTGCGAAAATCAATAAAATAAGAGCGCGCACCTTAAATCATTGAAAGGTTAATTAAAAAAATGCAAAACAATAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGATTTAAAGCAGtt             >  2:523030/1‑139 (MQ=255)
                                                                                                                              cGAAAATCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCg       <  2:777419/140‑1 (MQ=255)
                                                                                                                              cGAAAATCAATAAAATAAGACCCCCCACCTTTAATCCATGAAAGGATAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCg       <  1:1352431/140‑1 (MQ=255)
                                                                                                                                 aaaTCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCgtgt    <  1:1263760/140‑1 (MQ=255)
                                                                                                                                   aTCAATAAATTAAGTGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGAAAATCACTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTTACTTTAATTTCGACGCGTTAAAGCAGTATTAGCGTGTAg  <  2:1373972/140‑1 (MQ=255)
                                                                                                                                   aTCAATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCGTGTAg  <  2:1374449/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TAGTGGAATAAGGCAACGCCTGGATCTGCTGATCCGCTACGGTGATATCCTGATTATCGAAAAGGCTGGCGCGAAATGTATCGACCATACTTTGCTGGCTGTGCGTACATCCCGCAAGAAATAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCGTGTAG  >  NC_000913/1049179‑1049449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: