BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,238	T→A	5.9%	intergenic (+16/‑131)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,238	0	T	A	5.9%	543.7 / 4.6	169	intergenic (+16/‑131)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (92/62); new base A (9/4); total (103/66)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  NC_000913/781089‑781387

gagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACt                                                                                                                                                       >  2:130860/1‑150 (MQ=38)

   cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTATAGCAGTTTACTTTTAATCAATTGGTCGAAGGTTAGAATCCTGCACGAACAACCAGTTTTAACATCAAACTCAg                                                                                                                                                    >  2:939303/1‑150 (MQ=14)

   cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGATGGTAGAGCAGTTGACTTTTAATCTATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACAACAAACACAg                                                                                                                                                    >  2:2079087/1‑150 (MQ=255)

    aCCTCCCCTACAAGGCTGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                                                                                                                   <  1:541126/150‑1 (MQ=255)

     ccTCCCTTACTCGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTGAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAATCTCAGAt                                                                                                                                                  <  1:2355190/150‑1 (MQ=255)

     ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                                                                                                                  >  1:1475867/1‑150 (MQ=255)

     ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                                                                                                                  <  1:1436676/150‑1 (MQ=255)

       tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTTTTAACATCAAACTCAGATGt                                                                                                                                                >  1:2079304/1‑150 (MQ=255)

       tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTTTTAACATCAAACAAAGAttt                                                                                                                                                >  1:1702488/1‑148 (MQ=255)

        cccTTACAAGGTGGGGGTGGGCGGTTCGATCCCGGCATCACCCCCCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGtt                                                                                                                                               <  2:2652034/150‑1 (MQ=255)

           ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACGTCGAACGCAGAAGATaac                                                                                                                                            >  2:21406/1‑149 (MQ=11)

           ttACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGTTAAg                                                                                                                                            >  2:1450590/1‑150 (MQ=37)

                       ggTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATaa                                                                                                                                >  2:1603259/1‑150 (MQ=255)

                         tCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACg                                                                                                                              <  2:1937265/150‑1 (MQ=255)

                         gcgggggGTCGGTCCCGGCCTCCCCCCCCCCTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACg                                                                                                                              <  2:1764058/149‑1 (MQ=14)

                             cttttCTATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgc                                                                                                                          <  1:2125239/147‑1 (MQ=255)

                              ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAGTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGGATTAAAGTCAAACGCGGGTGGGAGGCGGGAAGGATAACGTGgcg                                                                                                                         >  2:713988/1‑150 (MQ=14)

                                 tCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                      <  2:1540471/150‑1 (MQ=255)

                                 tCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                      <  2:2664141/150‑1 (MQ=255)

                                    aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGTTAAGCGTGAAGCATAGCGTTGCGCCAGCa                                                                                                                   >  2:1992457/1‑150 (MQ=255)

                                     tCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCaa                                                                                                                  >  2:1249382/1‑150 (MQ=255)

                                           catcaCCCACCACTCGGGTCGTTTGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCACAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGccc                                                                                                            <  2:1408845/150‑1 (MQ=255)

                                               acccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAg                                                                                                        >  1:1755015/1‑150 (MQ=255)

                                               acccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCGAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGGAg                                                                                                        >  1:1406859/1‑150 (MQ=255)

                                                          gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGAGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaag                                                                                             >  2:382557/1‑150 (MQ=255)

                                                             tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTTGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                          >  1:1022063/1‑150 (MQ=255)

                                                             tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTTCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                          >  1:2542863/1‑150 (MQ=255)

                                                             tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                          >  1:2020632/1‑150 (MQ=255)

                                                             tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcga                                                                                          >  1:2372958/1‑150 (MQ=255)

                                                                ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTTGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCa                                                                                                                                                     <  1:441074/88‑1 (MQ=255)

                                                                ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAGTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTc                                                                                       >  1:857831/1‑150 (MQ=255)

                                                                ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCa                                                                                                                                                     >  2:1289989/1‑88 (MQ=39)

                                                                ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCa                                                                                                                                                     >  2:441112/1‑88 (MQ=39)

                                                                ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTc                                                                                       >  1:389304/1‑150 (MQ=255)

                                                                ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTc                                                                                       >  2:1894671/1‑150 (MQ=255)

                                                                  aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTg                                                                                                                           <  1:1133271/112‑1 (MQ=255)

                                                                  aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTg                                                                                                                           <  1:1265340/112‑1 (MQ=255)

                                                                     tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTAAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGACAAcca                                                                                  >  1:2565167/1‑149 (MQ=255)

                                                                     tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATAAAACTCAGATGTTAAGCGTGAAGGATAACGTTGAGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCt                                                                                  >  1:2365957/1‑150 (MQ=255)

                                                                           ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGa                                                                            >  2:84013/1‑150 (MQ=255)

                                                                           ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGa                                                                            >  2:1169334/1‑150 (MQ=255)

                                                                              agagCAGTTGACTTTTAATCAATTTGTCGCAGGTTCGAATCCAGCACGACCCACCAGTTTTAACATAAAAAAAAGATGTTAACCGTTAAGGATAACGTTGCGCCAGCAACGGCCCCTAGGGCGAGCGAAGCGAGTCAAACTGCaacaacc                                                                         >  1:941724/1‑144 (MQ=11)

                                                                              agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAccc                                                                         >  1:2259486/1‑150 (MQ=255)

                                                                              agagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCCCGTGAACCAGCACCAccc                                                                         >  1:2011382/1‑150 (MQ=14)

                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCCTATTAACATCAAACTCCTAACATAAGCGAGACGGATAACTTAGCGACAGCAACGGCCCGGAGGGAGAGCGAAGCGAGTCATCCTGCGCGACccaccc                                                                     >  1:1497915/1‑149 (MQ=14)

                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                     >  2:1600068/1‑150 (MQ=255)

                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                     <  2:1857314/150‑1 (MQ=255)

                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                     >  2:864358/1‑150 (MQ=255)

                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                     >  2:2215946/1‑150 (MQ=255)

                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca                                                                     >  2:321178/1‑150 (MQ=255)

                                                                                          ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             >  1:237041/1‑150 (MQ=255)

                                                                                          ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             >  1:1871460/1‑150 (MQ=255)

                                                                                          ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAAGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             >  1:1323278/1‑150 (MQ=255)

                                                                                           tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGc                                                                                                                                           >  1:1170559/1‑71 (MQ=255)

                                                                                           tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAg                                                            >  2:901360/1‑150 (MQ=255)

                                                                                               aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGAAGTTAAGCGTGAAGTATAACGTTCCGACAGAAACGACCCGAAGGGCGAGCGAATAGAGTCATACTGCACGACCAACCAAACTTAAAGAATg                                                        >  2:560963/1‑150 (MQ=14)

                                                                                               aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACGTCAAAATAGGAAGTTAACCGTGAAGGAAAACTTTCCGAAAGAAACGACCAGAATGGAGAGCGAAGCGAGTCATCATTAACGACACAAAAAACTTAAACAtag                                                        >  2:1080676/1‑148 (MQ=2)

                                                                                                  aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                     >  2:2136378/1‑150 (MQ=255)

                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATTTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGccc                                                    <  1:130860/150‑1 (MQ=255)

                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAACGATTGGccc                                                    <  2:2397195/150‑1 (MQ=255)

                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGccc                                                    >  2:2667479/1‑150 (MQ=255)

                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGccc                                                    >  1:246162/1‑150 (MQ=255)

                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGGccc                                                    >  1:2681903/1‑150 (MQ=255)

                                                                                                      ggTCGCTGTTTCGAATCCTGCACGACCCACCTGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                                                 <  1:452085/150‑1 (MQ=255)

                                                                                                          gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGACGGATAACGTGGCGCCAGCAACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAATCTTATAGATTGGCCCCGAgata                                             >  2:1084127/1‑147 (MQ=17)

                                                                                                          gCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGACGCGAGTCCTCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaa                                             >  1:2386473/1‑150 (MQ=255)

                                                                                                              gTTCGACTCCGGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                         <  1:2612530/150‑1 (MQ=255)

                                                                                                               ttCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATc                                        >  1:872765/1‑150 (MQ=255)

                                                                                                                  gAATCCTGCACGACCCACCAGTTTTATCTTCAAACTCCGCTGTTATGCGTGAAGTATTTTGTTGCGCCATCATCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     <  1:1635686/150‑1 (MQ=21)

                                                                                                                  gAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTATGCGTGAAGGATTAGGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     <  1:1723988/150‑1 (MQ=255)

                                                                                                                   aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                                                                                                                  <  1:1922722/40‑1 (MQ=255)

                                                                                                                   aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                                                                                                                  >  2:431611/1‑40 (MQ=255)

                                                                                                                   aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAt                                                                                                                                                  >  2:1922722/1‑40 (MQ=255)

                                                                                                                    acccTCCACGACCCACCAGAAATAACAACAAACACATATCTAACCCGTCAAGTATAAGGTTGAGCCAGCTTCGGACCGTAGTGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                   <  1:2412/148‑1 (MQ=16)

                                                                                                                    aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATTTTAAGCGTGAAGGATAAGGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                   <  1:745831/150‑1 (MQ=255)

                                                                                                                    aTCCTGAACGACCAACCAGTTTTAAAATCAAACTCAGATTTTAAGAGTGAAGGATATCGTTGCGCCATCTACGGCCAGTAGGGCGAGCGAAGCGATTCATCCTGCACGACCCACCAATATTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                   <  1:1969908/150‑1 (MQ=37)

                                                                                                                       cTGCACCACCCACCAGTTTTAACATCAATCTCAGTTTTTAATCTTGTTTGATATCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                <  1:1037316/150‑1 (MQ=21)

                                                                                                                        tGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTa                               >  1:189540/1‑150 (MQ=255)

                                                                                                                          cACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     <  2:166664/142‑1 (MQ=255)

                                                                                                                             gACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGGCCCCGAGTAAAAAACATACAGGTAacaca                          >  2:793575/1‑149 (MQ=255)

                                                                                                                                ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAACCGt                       >  1:377358/1‑150 (MQ=255)

                                                                                                                                  accaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     >  1:1932073/1‑134 (MQ=255)

                                                                                                                                  accaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     >  1:401832/1‑134 (MQ=14)

                                                                                                                                  accaGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                     <  2:401832/134‑1 (MQ=14)

                                                                                                                                  accaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAt                     <  2:1022063/150‑1 (MQ=255)

                                                                                                                                  accaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAt                     <  2:2542863/150‑1 (MQ=255)

                                                                                                                                  accaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAt                     >  1:255778/1‑150 (MQ=255)

                                                                                                                                   ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTTGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                    >  1:135583/1‑150 (MQ=255)

                                                                                                                                   ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATg                    <  2:2092341/150‑1 (MQ=255)

                                                                                                                                    caGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGAACGACCCACCAATCTTAAAGATAGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGAATgg                   >  1:1479462/1‑150 (MQ=255)

                                                                                                                                      gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGt                 <  1:1603157/150‑1 (MQ=255)

                                                                                                                                      gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGt                 <  2:2046179/150‑1 (MQ=255)

                                                                                                                                           aaCATCAACCTCAGAGGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTa            <  2:1475867/150‑1 (MQ=255)

                                                                                                                                           aaCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTa            <  2:2366112/150‑1 (MQ=255)

                                                                                                                                               tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc        >  2:2392/1‑150 (MQ=255)

                                                                                                                                                 aaaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACACGTATGGGTCGTTAGCTCAg      >  2:2684922/1‑150 (MQ=255)

                                                                                                                                                   aCTCAGCTGTTCAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt    <  1:561899/150‑1 (MQ=255)

                                                                                                                                                   aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt    <  1:1598207/150‑1 (MQ=255)

                                                                                                                                                   aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt    <  1:1350148/150‑1 (MQ=255)

                                                                                                                                                     tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  <  1:2625941/150‑1 (MQ=255)

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  NC_000913/781089‑781387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`