Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 940,751 T→C 8.1% intergenic (+31/‑208) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913940,7510TC 8.1% 456.9 / 24.2 148intergenic (+31/‑208)serS/dmsAseryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (65/71);  new base C (6/6);  total (71/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

Only 100 of 149 total aligned reads displayed.

GTCTGGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAA  >  NC_000913/940602‑940898
                                                                                                                                                     |                                                                                                                                                   
gtctggCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCt                                                                                                                                                     >  2:2652134/1‑150 (MQ=255)
     gCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCggg                                                                                                                                                >  2:345414/1‑150 (MQ=255)
           ggTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttt                                                                                                                                          >  1:2583224/1‑150 (MQ=255)
              cgaaCGCTGGTTCCAGAAATGCAAAACTATCACCAGGCTGATGGTCGAATTCAAGTACCAGAAGTTCTGCCACCGAATATGAACGCACTGGAATATATTGCCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                                                                       <  2:545513/147‑2 (MQ=255)
              cGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCCGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTCCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                                                                       <  2:39914/150‑2 (MQ=255)
               gTACGCTGGTTGCCGTAATGGAAAACTATCCGCCGGCTGATGGTCGTATTGAAGTACCAGACGTTCGGCGTCCGTATATGAACGGACTGGAATATTTTGGCTAATACCCAATTTTTCTGAATCTAAACAGCGCCTGCGGGCGCttttttt                                                                                                                                      <  1:2642513/150‑1 (MQ=255)
               gTACGCTGGTTGCAGTAATTGAAAACTTTCAGAAGGCTGATGGTCGTATTGATGTTCCAGTTGTTCTGGGTCCGTATATGACCGGACTGTAATATATTGGCTACTACCCAATTTTTCTGAATCTTACAAGCGCCTGCGGGCGCttttttt                                                                                                                                      <  2:1588054/150‑1 (MQ=255)
               gTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttttttt                                                                                                                                      <  2:1255449/150‑1 (MQ=255)
                tACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTg                                                                                                                                     <  2:1799244/150‑1 (MQ=255)
                 aCGCTGGGTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGt                                                                                                                                    <  2:2580775/150‑1 (MQ=255)
                  cGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagatt                                                                                                                                   <  2:2553649/150‑6 (MQ=255)
                  cGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagatt                                                                                                                                   <  2:2335509/150‑6 (MQ=255)
                    cTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                                                                 <  2:1160719/150‑1 (MQ=255)
                     tGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCATAAGTTCTGCGTCAGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAATCGCCTGCGGGCGCTTTTTTTGtatac                                                                                                                                >  2:2459021/1‑146 (MQ=255)
                     tGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTcc                                                                                                                                >  2:1377086/1‑150 (MQ=255)
                         tGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCttt                                                                                                                            <  1:1535477/150‑1 (MQ=255)
                         tGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCttt                                                                                                                            <  1:1367068/150‑1 (MQ=255)
                            aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTGTGCCTTTgtt                                                                                                                         >  2:699944/1‑148 (MQ=255)
                            aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                         >  2:2107113/1‑150 (MQ=255)
                            aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                         >  1:1165643/1‑150 (MQ=255)
                            aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                         >  2:463766/1‑150 (MQ=255)
                            aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAt                                                                                                                         >  2:2106009/1‑150 (MQ=255)
                            aGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTACCTTTGAt                                                                                                                         >  2:2353483/1‑150 (MQ=255)
                               aaTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAcc                                                                                                                      <  2:440864/150‑1 (MQ=255)
                                aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAACAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                     <  2:2750029/150‑1 (MQ=255)
                                aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                     <  2:205210/150‑1 (MQ=255)
                                aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                     <  2:1731315/150‑1 (MQ=255)
                                aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                     <  2:1731356/150‑1 (MQ=255)
                                aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                     <  2:544078/150‑1 (MQ=255)
                                    aaaaCTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACa                                                                                                                 <  1:1111770/150‑1 (MQ=255)
                                         tATCAGCAGGCTGTTGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataat                                                                                                            <  1:1900224/150‑1 (MQ=255)
                                         tATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataat                                                                                                            >  1:2295113/1‑150 (MQ=255)
                                         tATCAGCAGGCTGATGGTCGTATTGAAGTACAAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAACGAACaataat                                                                                                            >  1:994845/1‑150 (MQ=255)
                                          aTCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAAtt                                                                                                           <  1:124297/150‑1 (MQ=255)
                                                agGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcct                                                                                                     <  1:2002199/150‑1 (MQ=255)
                                                    tGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACt                                                                                                 >  2:2426593/1‑150 (MQ=255)
                                                      aTGGTCGTATTGAAGTACCAGATGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTa                                                                                               <  1:1626298/150‑1 (MQ=255)
                                                      aTGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTa                                                                                               <  2:1772596/150‑1 (MQ=255)
                                                      aTGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTa                                                                                               <  1:1166210/150‑1 (MQ=255)
                                                        ggTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTaca                                                                                             <  1:1039418/150‑1 (MQ=255)
                                                          tCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACg                                                                                           >  2:1534530/1‑150 (MQ=255)
                                                               ttGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTACCATTGATAACGAACAATAAATACTCATAACATACACGAAAta                                                                                      >  1:1014496/1‑150 (MQ=255)
                                                                tGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtac                                                                                     <  1:1904896/150‑1 (MQ=255)
                                                                     tACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCATTGATACCGAACAAAAAATAAAACACACTTACACGAAATAAAACtt                                                                                >  1:2459636/1‑150 (MQ=255)
                                                                         aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                            >  2:849810/1‑150 (MQ=255)
                                                                         aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                            <  1:2142205/150‑1 (MQ=255)
                                                                         aGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCATTGATACCGAACAAAAATTACTCCTCACATACACCTAATACTACTTTCGa                                                                            >  2:1037460/1‑150 (MQ=255)
                                                                              ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGTTACCGAACCAAAATTACTCCTCACTTACACGTGATAATACTTTAGAGTGaa                                                                       >  1:1879117/1‑150 (MQ=255)
                                                                              ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGAAATACTAAGTTCGAGTGaa                                                                       >  2:2498845/1‑150 (MQ=255)
                                                                              ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCGTTGATCCCGAACAATAATTACTCCTCACTTACACGTAATACGACTTTCGAGTGaa                                                                       >  2:2672216/1‑150 (MQ=255)
                                                                               tCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTATCCCGTTGATAAAGAACAAAAAATAATACTCACATAAAACTAAAACAACATTAGAGTGaaa                                                                      >  1:22656/1‑150 (MQ=255)
                                                                                 tGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAAt                                                                    <  2:253861/150‑1 (MQ=255)
                                                                                 tGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAAt                                                                    <  2:2583224/150‑1 (MQ=255)
                                                                                  gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCTTTGATAACGAACAAAAATTACTCCTCACTTACACGTAAAACAACATTCGAGTGaaaaac                                                                   >  1:178912/1‑148 (MQ=255)
                                                                                  gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCGTTGATAACGAAAAATAATTACAACACACATACACGAAATACAAAGATCGAGTTTAAATc                                                                   >  1:875609/1‑150 (MQ=255)
                                                                                  gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTAATACACACTTACACGTAATAATAAATTCGAGTGAAAATc                                                                   >  1:1360071/1‑150 (MQ=255)
                                                                                  gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCGTTGATACCGCACAATAATTACTCCTCACTTACACGGAATACTACGTTCGAGTGAAAATc                                                                   >  1:1545007/1‑150 (MQ=255)
                                                                                    gTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTAATTTCGAGTGTAAATCTa                                                                 >  2:582795/1‑150 (MQ=255)
                                                                                            atGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATctct                                                        >  1:1597174/1‑150 (MQ=255)
                                                                                                  ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                   <  2:2240518/150‑1 (MQ=255)
                                                                                                  ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                   <  2:816758/150‑1 (MQ=255)
                                                                                                  ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                   <  2:1339559/150‑1 (MQ=255)
                                                                                                  ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                   <  1:2127077/150‑1 (MQ=255)
                                                                                                  ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                   <  2:1452597/150‑1 (MQ=255)
                                                                                                  ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                   <  1:2545914/150‑1 (MQ=255)
                                                                                                   gACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTGTCCCGTTGTTACCGAACAAAAAATACTCCACACATACACGGAATACTACATTCGAGTTTAAATCTAACTATCTCTTTGAtt                                                  >  1:574238/1‑150 (MQ=255)
                                                                                                   gACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCGTTTTTTGTATACCATTTATACCCAAAAAAAAATACTCCACAAAAACACGGAAAACAAAATTAGAGTGAAAAACTACCGATAACTTTGAtt                                                  >  1:1336046/1‑150 (MQ=255)
                                                                                                     cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAtttt                                                <  1:2107113/150‑1 (MQ=255)
                                                                                                     cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAtttt                                                <  1:2106009/150‑1 (MQ=255)
                                                                                                     cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAtttt                                                <  1:699944/150‑1 (MQ=255)
                                                                                                     cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAtttt                                                <  1:699947/150‑1 (MQ=255)
                                                                                                      tGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTATCCCTTTGATACCGCAAAAAAATTAATCCTCACATACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTc                                               >  2:2182367/1‑150 (MQ=255)
                                                                                                          atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat                                           <  2:2022085/150‑1 (MQ=255)
                                                                                                          atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAat                                           <  2:780729/150‑1 (MQ=255)
                                                                                                           tatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCGTTTTTTGTCTCCCATTGATACCCAACAAAAAATAATACACACAGACACCTAAAAATACATTAGAGTGAAAATAAACCAATATAGTTTATTTTaaaaat                                          >  1:1473208/1‑148 (MQ=255)
                                                                                                             tatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAAAAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAAACTACCTATCTCTTTGATTTTCAAattat                                        >  2:2480196/1‑150 (MQ=255)
                                                                                                                 ggCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGa                                    <  1:37441/150‑1 (MQ=255)
                                                                                                                 ggCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGa                                    <  1:345414/150‑1 (MQ=255)
                                                                                                                 ggCTAATACCAAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGa                                    <  2:2490472/150‑1 (MQ=255)
                                                                                                                         cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTAAAATTATTCGATGGATACa                            >  2:1113607/1‑150 (MQ=255)
                                                                                                                         cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGGCTCCCTTTGATCCCCAACAATAATTACTCCACACTTACACGGAATACGACTTTCGAGTGAAAATCTACCTATCTCATTGATTTTCAAATTATTCGATGTATACa                            >  2:419668/1‑150 (MQ=255)
                                                                                                                          ccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACaa                           >  2:1152699/1‑150 (MQ=255)
                                                                                                                           cAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc                         <  2:1597174/150‑1 (MQ=255)
                                                                                                                            aaTTTTTCTGAATCTCAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTGGATACCGAACAATAATTACTCCTCACTTACACGTAATACTCCTTTCGAGTGAAAATCTACCTCTCACTTTGATTTTCAAATTATTCGATGTATACAAGc                         <  2:2292442/150‑1 (MQ=255)
                                                                                                                            aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc                         <  1:32948/150‑1 (MQ=255)
                                                                                                                            aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc                         <  2:441451/150‑1 (MQ=255)
                                                                                                                               ttttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATTCCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta                      >  2:2521260/1‑150 (MQ=255)
                                                                                                                                   cTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCTTTGATACCGAACAAAAATTAATCCTCACTTAAAAGTAATAAAAAATTCGAGTGAAAATATACCAATATCTTTTATTTTAAAATTATTAGATTTATAAAAGACaatata                  >  2:1856025/1‑150 (MQ=255)
                                                                                                                                   cTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtatata                  >  2:1138058/1‑150 (MQ=255)
                                                                                                                                     gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc                <  1:1292264/150‑1 (MQ=255)
                                                                                                                                     gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc                <  1:1107957/150‑1 (MQ=255)
                                                                                                                                        caaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa             >  2:2375600/4‑150 (MQ=255)
                                                                                                                                         aaaaaaaGCGCCCGCAGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACt           >  2:447251/3‑150 (MQ=255)
                                                                                                                                           aaaaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTg          <  1:593513/150‑1 (MQ=255)
                                                                                                                                            aaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc         >  2:74112/1‑150 (MQ=255)
                                                                                                                                              aaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                            >  2:1964077/1‑81 (MQ=255)
                                                                                                                                              aaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGa                                                                            <  1:1964077/81‑1 (MQ=255)
                                                                                                                                                 cgcCTGCGGGCGCTTTTTTTTTCTCCCTTTTATACCGAACAATAATTAATCCACAATTACACGTAATACTACATTCGTGTGAAAATCTACCAATCTATTTTATTTTAAAATTATTCGATGTATACAAGCCGATATAGCGAACTGCTATAg    >  2:1067800/1‑150 (MQ=255)
                                                                                                                                                  gcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGa   <  1:1022471/150‑1 (MQ=255)
                                                                                                                                                   ccTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaa  <  1:2468876/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTCTGGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAA  >  NC_000913/940602‑940898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: