Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,438 T→G 6.8% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,4380TG 6.8% 278.7 / 7.7 93pseudogene (1047/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base T (41/45);  new base G (2/5);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTT  >  NC_000913/1466289‑1466585
                                                                                                                                                     |                                                                                                                                                   
gTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGtt                                                                                                                                                     >  2:2581865/1‑150 (MQ=255)
  cAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTAt                                                                                                                                                   <  1:573630/150‑1 (MQ=255)
      aaCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAg                                                                                                                                               >  1:2088429/1‑150 (MQ=255)
          aaGGCGAGATCTCCATCTCCAACGGTGTCACCGTGACAGAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACg                                                                                                                                           <  2:2094430/150‑1 (MQ=17)
           aGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACgg                                                                                                                                          <  1:1026497/150‑1 (MQ=255)
            ggCGAGATCACCATCACCAACGGTGGCCCAGGCACCGAGATTACTGGTGATGACGCCACTGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGc                                                                                                                                         <  2:1201795/150‑1 (MQ=17)
               gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGAt                                                                                                                                      >  1:1009800/1‑150 (MQ=255)
               gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGAt                                                                                                                                      >  1:2551142/1‑150 (MQ=255)
               gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGAt                                                                                                                                      >  1:1562095/1‑150 (MQ=255)
               gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGAt                                                                                                                                      >  1:1463235/1‑150 (MQ=255)
                 gagCACCATCAACAACGCTGGCACAGGCACCGAGATTACTGGTGATGACGCCACTGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCt                                                                                                                                    <  2:2512890/150‑1 (MQ=17)
                  agCACCATCACCAACGGTGGCACCGGCACCCAGATTACTGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTg                                                                                                                                   <  1:2149431/150‑1 (MQ=255)
                  agCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTg                                                                                                                                   <  1:1117399/150‑1 (MQ=255)
                  agCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTg                                                                                                                                   <  1:2823/150‑1 (MQ=255)
                    cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCACACAACAAAGGCAAAACCACCGTTGACGGCAAGGATTCCACAGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                                                                                                                                 >  1:1436048/1‑150 (MQ=255)
                    cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTCCGGAAATCAACGGTAATAACGGAAATGTTATTCGGGACGGCGATCTGGa                                                                                                                                 >  1:1241461/1‑150 (MQ=255)
                    cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGTAAAGTTATTCAGGACGGCGATCTGGa                                                                                                                                 >  1:1172967/1‑150 (MQ=255)
                    cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                                                                                                                                 >  1:1241460/1‑150 (MQ=255)
                     aCCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt                                                                                                                                >  1:1101805/1‑150 (MQ=255)
                     aCCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt                                                                                                                                <  2:769304/150‑1 (MQ=255)
                       cATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACTGACAAGTTATTCAGGACGGCGATCTGGATGt                                                                                                                              >  2:1485376/1‑150 (MQ=255)
                           aCCAACTGTTGCACCGGCACCCAGAATAAAGGTGATGACGCCACCGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgc                                                                                                                          <  2:2187108/150‑1 (MQ=17)
                           aCCAACGGTGGCACCGCCACACAGAATTACGCTGATCACGCCACCGAAAACAACAACGGCAAAACCACCGTTGACGGCAACGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgc                                                                                                                          <  2:2566933/150‑1 (MQ=14)
                              aaCGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggc                                                                                                                       <  2:2429020/150‑1 (MQ=255)
                                cGGTTGCACCGTCACCCAGAGTAATGGAGTCGACTCCTCTCCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcgg                                                                                                                     <  2:2183047/150‑1 (MQ=17)
                                  gTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggcg                                                                                                                   <  2:1101805/150‑1 (MQ=255)
                                         cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg                                                                                                            <  1:2581865/150‑1 (MQ=255)
                                                     tAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc                                                                                                <  1:908026/150‑1 (MQ=255)
                                                     tAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc                                                                                                <  2:877796/150‑1 (MQ=255)
                                                                 cGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAgcgc                                                                                    <  1:1628734/150‑1 (MQ=255)
                                                                 cGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAgcgc                                                                                    <  1:1715147/150‑1 (MQ=255)
                                                                     aCGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACg                                                                                >  1:774954/1‑150 (MQ=255)
                                                                     aCGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTACACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACg                                                                                >  1:774973/1‑150 (MQ=255)
                                                                      cGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGAAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACgg                                                                               >  1:315104/1‑150 (MQ=255)
                                                                       tgtaaaCAACAATGGCAAAACCACCGTTGACGGCAACGATTCCACCGGTACGGAAATCAACGGTAATAACGGTAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGt                                                                              <  2:1814173/147‑1 (MQ=11)
                                                                       cgtaaaCAACAACGGCAAAACCACCGTTGACGGGAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGt                                                                              <  2:2338379/147‑1 (MQ=255)
                                                                        gCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTg                                                                             >  2:1443974/1‑150 (MQ=255)
                                                                          aaaCAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGa                                                                           >  1:1282776/1‑150 (MQ=255)
                                                                           aacaacaacGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAt                                                                          >  1:1195441/1‑150 (MQ=255)
                                                                             caacaacGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCTACGGTAATAACGGTAACGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt                                                                                                         <  2:2722426/117‑1 (MQ=255)
                                                                             caacaacGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt                                                                                                         >  1:2722426/1‑117 (MQ=255)
                                                                               acaacGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACa                                                                      >  1:244910/1‑150 (MQ=255)
                                                                               acaacGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATAAACGGTAATAACGGGAAGGTTATTCAGTACGGCGATCTGTATGTCAGCGGAGGCGTTAACTGTATTGATATCAACGGCGAGGGCGCAACGGTGGATaaaa                                                                      >  2:950406/1‑148 (MQ=11)
                                                                                  acGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAATGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGTCGACCGCGCTACTGAGCAAAACaaag                                                                   >  1:1615139/1‑148 (MQ=14)
                                                                                  acGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa                                                                                                      <  2:1614815/115‑1 (MQ=255)
                                                                                  acGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa                                                                                                      >  1:1614815/1‑115 (MQ=255)
                                                                                                  tGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCAc                                                   <  1:2082988/150‑1 (MQ=255)
                                                                                                         aaGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                            <  2:244807/150‑1 (MQ=255)
                                                                                                         aaGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                            <  2:2414927/150‑1 (MQ=255)
                                                                                                           ggATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGa                                          >  2:465520/1‑150 (MQ=40)
                                                                                                                ccACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                     >  1:2506685/1‑150 (MQ=39)
                                                                                                                    cGGTACGGAAATAAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCTGCGGCGGCGGTCACGGTTTTGATATCACCGGGGACAGCGCAACGTTGGATAACAAGGGAACATGACCGTCACCGATCCGGAGTCCATCGGt                                <  2:1787951/150‑1 (MQ=14)
                                                                                                                     ggTACGGAAATCAACGGTAATAACGGTAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCa                                                    <  1:118881/130‑1 (MQ=17)
                                                                                                                         cGGAAATCAACGGTAATAACGGTAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATcc                            <  1:2434626/150‑1 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGTAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  1:1798570/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCACCGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  1:996481/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  2:2604166/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  2:491172/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  2:2378116/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  2:861625/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  2:955794/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  2:1798570/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  2:996481/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  2:1509430/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  2:1509319/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  1:1509319/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  1:1509430/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  1:2378116/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         <  1:2604166/149‑2 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  1:955794/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  1:861625/1‑148 (MQ=255)
                                                                                                                             aaTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAga                         >  1:491172/1‑148 (MQ=255)
                                                                                                                                cAACGGTAATAACGGATAAGTTATTCAGGACGGCGATCTGGATGTCTGCGGCGGCGGTAACGGTATTGATATCACCGGCGACAGCGCAACGGTGTATTACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGa                     <  1:1485376/150‑1 (MQ=14)
                                                                                                                                   cGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACgg                  >  1:1193681/1‑150 (MQ=17)
                                                                                                                                      taataaCGGTAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGa               <  1:465520/150‑1 (MQ=17)
                                                                                                                                      taataaCGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGa               <  2:825030/150‑1 (MQ=11)
                                                                                                                                       aataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc              <  2:2551142/150‑1 (MQ=39)
                                                                                                                                       aataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc              <  2:1562095/150‑1 (MQ=39)
                                                                                                                                       aataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc              <  2:1009800/150‑1 (MQ=39)
                                                                                                                                       aataaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAc              <  2:1463235/150‑1 (MQ=39)
                                                                                                                                         taaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCa            >  1:2645755/1‑150 (MQ=39)
                                                                                                                                         taaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCa            >  1:2174689/1‑150 (MQ=39)
                                                                                                                                         taaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCa            >  1:2174686/1‑150 (MQ=39)
                                                                                                                                          aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACCGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAg           >  1:2153712/1‑150 (MQ=21)
                                                                                                                                          aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAg           >  1:2160454/1‑150 (MQ=21)
                                                                                                                                             gggaaaGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCg        >  2:1195858/4‑150 (MQ=11)
                                                                                                                                             ggAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCATCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGcc        >  1:393756/1‑149 (MQ=18)
                                                                                                                                             ggAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGcc        >  2:1234719/1‑149 (MQ=18)
                                                                                                                                              ggaaaGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCgg       <  2:332607/148‑1 (MQ=17)
                                                                                                                                              gAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCAcc                                                              <  2:1659558/95‑1 (MQ=39)
                                                                                                                                              gAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCAcc                                                              >  1:1659558/1‑95 (MQ=39)
                                                                                                                                                  aGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttgt   <  2:1195441/150‑1 (MQ=40)
                                                                                                                                                  aGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCTACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttgt   <  2:455672/150‑1 (MQ=255)
                                                                                                                                                   gTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttgtt  <  2:2506685/150‑1 (MQ=40)
                                                                                                                                                    ttATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGa                                                          >  2:1901834/1‑93 (MQ=32)
                                                                                                                                                     |                                                                                                                                                   
GTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTT  >  NC_000913/1466289‑1466585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: