Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,701,024 A→T 5.0% D23V (GAC→GTC)  malY → PLP‑dependent beta‑cystathionase and maltose regulon regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,701,0240AT 5.0% 569.6 / 14.0 160D23V (GAC→GTC) malYPLP‑dependent beta‑cystathionase and maltose regulon regulator
Reads supporting (aligned to +/- strand):  ref base A (80/71);  new base T (7/2);  total (87/73)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

GGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGC  >  NC_000913/1700875‑1701171
                                                                                                                                                     |                                                                                                                                                   
ggTTTTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGa                                                                                                                                                     <  1:394600/150‑1 (MQ=255)
ggTTGTTATCGGGCCACACGTTCAGTCAGTAAAAGATGAACTGGCCGGTCTGATGCAGACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCCAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGa                                                                                                                                                     <  2:898621/150‑1 (MQ=255)
ggTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGa                                                                                                                                                     <  2:153907/150‑1 (MQ=255)
ggTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGa                                                                                                                                                     <  1:885393/150‑1 (MQ=255)
ggTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGa                                                                                                                                                     <  1:1146084/150‑1 (MQ=255)
ggTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGa                                                                                                                                                     <  1:144359/150‑1 (MQ=255)
 gttgttATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGAc                                                                                                                                                    >  2:2202945/1‑150 (MQ=255)
 gttgttATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGAc                                                                                                                                                    <  1:2735116/150‑1 (MQ=255)
 gttgttATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGAc                                                                                                                                                    <  1:1979385/150‑1 (MQ=255)
  gtgttATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGAcc                                                                                                                                                   <  1:1035725/149‑1 (MQ=255)
   tgttATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCg                                                                                                                                                  >  1:232654/1‑150 (MQ=255)
      tATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGttt                                                                                                                                               >  2:2651467/1‑150 (MQ=255)
      tATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGttt                                                                                                                                               >  1:2735027/1‑150 (MQ=255)
      tATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGttt                                                                                                                                               >  1:975171/1‑150 (MQ=255)
       aTCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTc                                                                                                                                              <  2:146935/150‑1 (MQ=255)
       aTCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTc                                                                                                                                              <  2:572957/150‑1 (MQ=255)
       aTCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTc                                                                                                                                              <  2:825931/150‑1 (MQ=255)
            gCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCAc                                                                                                                                         <  2:379477/150‑1 (MQ=255)
                caAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCActgct                                                                                                                                     <  2:2516545/150‑1 (MQ=255)
                  aGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGa                                                                                                                                   <  2:2102138/150‑1 (MQ=255)
                   gTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATTCTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGAc                                                                                                                                  <  1:2662759/150‑1 (MQ=255)
                         tcagtAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTa                                                                                                                            <  1:63926/150‑1 (MQ=255)
                             tAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGt                                                                                                                        <  2:1949804/150‑1 (MQ=255)
                               aaaGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTc                                                                                                                      >  1:1646912/1‑150 (MQ=255)
                                aaGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCa                                                                                                                     <  2:2593714/150‑1 (MQ=255)
                                      aaaTGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGAttt                                                                                                               >  2:2504594/1‑150 (MQ=255)
                                             cGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACAt                                                                                                        <  1:1418751/150‑1 (MQ=255)
                                             cGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACAt                                                                                                        <  1:1244125/150‑1 (MQ=255)
                                             cGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACAt                                                                                                        <  1:2318644/150‑1 (MQ=255)
                                                 cTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGAt                                                                                                    <  2:1055884/150‑1 (MQ=255)
                                                      gCATACTGTCCATGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGGTACCGTTCACGATTTCAGACATGGATTTTGc                                                                                               <  1:1112665/150‑1 (MQ=255)
                                                      gCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGc                                                                                               >  2:2114974/1‑150 (MQ=255)
                                                      gCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGc                                                                                               >  2:736472/1‑150 (MQ=255)
                                                            tGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGc                                                                                         <  2:299792/150‑1 (MQ=255)
                                                            tGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGc                                                                                         <  1:2166430/150‑1 (MQ=255)
                                                            tGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGc                                                                                         <  2:1746605/150‑1 (MQ=255)
                                                            tGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGc                                                                                         <  2:607233/150‑1 (MQ=255)
                                                                   gCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGc                                                                                  >  1:2461487/1‑150 (MQ=255)
                                                                   gCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGc                                                                                  >  1:1538116/1‑150 (MQ=255)
                                                                           aTAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGa                                                                          <  1:1487700/150‑1 (MQ=255)
                                                                           aTAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGa                                                                          <  1:1906757/150‑1 (MQ=255)
                                                                           aTAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGa                                                                          <  2:498504/150‑1 (MQ=255)
                                                                           aTAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGa                                                                          <  2:394804/150‑1 (MQ=255)
                                                                           aTAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGa                                                                          <  1:1568031/150‑1 (MQ=255)
                                                                             aaGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAgg                                                                        >  2:2137568/1‑150 (MQ=255)
                                                                              aGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGgc                                                                       >  1:169352/1‑150 (MQ=255)
                                                                              aGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGgc                                                                       >  1:1094214/1‑150 (MQ=255)
                                                                              aGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGgc                                                                       >  1:1816448/1‑150 (MQ=255)
                                                                              aGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGgc                                                                       >  1:32329/1‑150 (MQ=255)
                                                                                atatGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGgcgc                                                                     >  2:506225/1‑150 (MQ=255)
                                                                                     ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAAt                                                                >  2:2123031/1‑150 (MQ=255)
                                                                                     ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAAt                                                                >  2:766235/1‑150 (MQ=255)
                                                                                     ttCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAAt                                                                >  2:2397479/1‑150 (MQ=255)
                                                                                        gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTGGCTGACCGTTTCGGCGCTGCTGACCTGTTACCGTTCACGATGTCAGACATGGAGTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAg                                                             >  2:1400585/1‑150 (MQ=255)
                                                                                        gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAg                                                             >  2:125143/1‑150 (MQ=255)
                                                                                        gATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAg                                                             >  2:1172867/1‑150 (MQ=255)
                                                                                           ttttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAgcgc                                                          <  1:466308/150‑1 (MQ=255)
                                                                                            tttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGcc                                                         >  1:1908753/1‑150 (MQ=255)
                                                                                            tttCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGcc                                                         >  1:2529899/1‑150 (MQ=255)
                                                                                              tCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGACTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTg                                                       >  2:2565953/1‑150 (MQ=255)
                                                                                               cAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGa                                                      <  2:1514838/150‑1 (MQ=255)
                                                                                               cAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGa                                                      <  2:361462/150‑1 (MQ=255)
                                                                                                aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGAt                                                     >  2:932863/1‑150 (MQ=255)
                                                                                                aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGAt                                                     >  2:75110/1‑150 (MQ=255)
                                                                                                aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGAt                                                     >  1:1079278/1‑150 (MQ=255)
                                                                                                aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGAt                                                     >  2:406255/1‑150 (MQ=255)
                                                                                                aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGAt                                                     >  1:1079178/1‑150 (MQ=255)
                                                                                                aaaGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGAt                                                     >  1:1072395/1‑150 (MQ=255)
                                                                                                aaaGGACGTGGAACGTCATGGCACATGGTGTAAACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCAGTTACAGTTCACGATTTAAGACATGCATTTTGACACAGCCCCCAGCATTATCGACGCGCTGAAACAGATCCTGAt                                                     >  2:1770286/1‑150 (MQ=255)
                                                                                                  aGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGc                                                   >  1:2690897/1‑150 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCTCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACAAGGATTTAGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGAACg                                                >  2:2384924/1‑150 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                >  2:562527/1‑150 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                <  2:584912/150‑1 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                <  1:1420001/150‑1 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                >  2:2351406/1‑150 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                >  2:2163753/1‑150 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                <  2:81803/150‑1 (MQ=255)
                                                                                                     tCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACg                                                <  2:1825995/150‑1 (MQ=255)
                                                                                                      cGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACgg                                               <  2:2194534/150‑1 (MQ=255)
                                                                                                       gTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGc                                              >  1:1020606/1‑150 (MQ=255)
                                                                                                       gTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGc                                              >  1:1782312/1‑150 (MQ=255)
                                                                                                         ggATCGTCATGGCTCATGGTGTACACAGTGGGATTATGTCGCGGACCGTTTCGGCACGGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACTGCGt                                            <  2:2108878/150‑1 (MQ=255)
                                                                                                            tCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTAtt                                         >  1:2257134/1‑150 (MQ=255)
                                                                                                               tCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTgg                                      <  2:1137031/150‑1 (MQ=255)
                                                                                                                    gCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACa                                 >  2:2020006/1‑150 (MQ=255)
                                                                                                                       caTGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGcc                              <  1:549018/150‑1 (MQ=255)
                                                                                                                       caTGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGcc                              <  1:343053/150‑1 (MQ=255)
                                                                                                                          ggTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCt                           >  1:730089/1‑150 (MQ=255)
                                                                                                                           gtgtACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTg                          <  2:2535109/150‑1 (MQ=255)
                                                                                                                              tACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaa                       >  1:778722/1‑150 (MQ=255)
                                                                                                                                 acaGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGaaaaa                    >  1:79717/1‑150 (MQ=255)
                                                                                                                                  caGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAAc                   <  2:1411365/150‑1 (MQ=255)
                                                                                                                                             tGTCGCTGTCCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct        >  2:2711435/1‑150 (MQ=255)
                                                                                                                                             tGTCGCTGTCCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct        >  2:2291492/1‑150 (MQ=255)
                                                                                                                                             tGTCGCTGTCCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct        >  2:1730569/1‑150 (MQ=255)
                                                                                                                                             tGTCGCTGTCCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct        >  2:1552926/1‑150 (MQ=255)
                                                                                                                                             tGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct        >  1:160812/1‑150 (MQ=255)
                                                                                                                                             tGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtct        >  1:286101/1‑150 (MQ=255)
                                                                                                                                              gTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTAAGACATGGATTTTGTCACTGCCCCCTGCATTATAGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTtctc       >  1:2594376/1‑150 (MQ=255)
                                                                                                                                                   tGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGc  <  1:147996/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGTTGTTATCGGGCCACAAGTTCAGTCAGTAAAAGATGAAATGGCCGGTCTGATGCATACTGTCCAGGCATAAGGATAAGATATGTTCGATTTTTCAAAGGTCGTGGATCGTCATGGCACATGGTGTACACAGTGGGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGC  >  NC_000913/1700875‑1701171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑