Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,091,575 G→A 7.4% C160Y (TGC→TAC)  hisD → bifunctional histidinal dehydrogenase/ histidinol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,091,5750GA 7.4% 541.3 / 21.5 161C160Y (TGC→TAC) hisDbifunctional histidinal dehydrogenase/ histidinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (82/67);  new base A (6/6);  total (88/73)
Fisher's exact test for biased strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

AACGCAGCCAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGG  >  NC_000913/2091426‑2091724
                                                                                                                                                     |                                                                                                                                                     
aaCGCAGCCAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGgtgctgtg                                                                                                                                                       >  1:2673252/1‑150 (MQ=255)
aaCGCAGCCAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGgtgctgtg                                                                                                                                                       >  2:194755/1‑150 (MQ=255)
 aCGCAGCCAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGgtgctgtgc                                                                                                                                                      >  1:708595/1‑150 (MQ=255)
 aCGCAGCCAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGgtgctgtgc                                                                                                                                                      >  1:146143/1‑150 (MQ=255)
  cGCAGCCAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCGGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATTCTGGCGACTCCGGCGAGTATTGCGGTCTGTAAAATAGTGgtgctgtgct                                                                                                                                                     <  1:1134062/150‑1 (MQ=255)
       ccAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCAccg                                                                                                                                                >  1:295776/1‑150 (MQ=255)
        cAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCAccgc                                                                                                                                               >  1:2331313/1‑150 (MQ=255)
           gcgtgcgtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCAccgccgc                                                                                                                                            >  2:2579865/1‑150 (MQ=255)
           gcgtgcgtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCAccgccgc                                                                                                                                            >  1:1894802/1‑150 (MQ=255)
                cgtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTACTGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGAtt                                                                                                                                       <  2:404918/150‑1 (MQ=255)
                cgtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGAtt                                                                                                                                       <  2:1402450/150‑1 (MQ=255)
                cgtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGAtt                                                                                                                                       <  2:1301359/150‑1 (MQ=255)
                cgtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGAtt                                                                                                                                       <  2:1739157/150‑1 (MQ=255)
                cgtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGAtt                                                                                                                                       <  2:2482007/150‑1 (MQ=255)
                 gtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTg                                                                                                                                      >  1:1988105/1‑150 (MQ=255)
                 gtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTg                                                                                                                                      >  2:246847/1‑150 (MQ=255)
                 gtTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTg                                                                                                                                      >  2:1128554/1‑150 (MQ=255)
                   tGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGcc                                                                                                                                    <  1:456188/150‑1 (MQ=255)
                       agcagGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATg                                                                                                                                >  2:1424229/1‑150 (MQ=255)
                        gcagGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATga                                                                                                                               >  1:1290866/1‑150 (MQ=255)
                            gTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCAGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATc                                                                                                                           >  1:1724176/1‑150 (MQ=255)
                               aCGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCtt                                                                                                                        <  2:1061027/150‑1 (MQ=255)
                                      cGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATgcgg                                                                                                                 <  1:373210/150‑1 (MQ=255)
                                      cGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATgcgg                                                                                                                 <  1:1028605/150‑1 (MQ=255)
                                        gTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTACTCACCGCCGCCGATTGCCGATGAGATCCTTTATgcggcg                                                                                                               >  2:1671833/1‑150 (MQ=255)
                                        gTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTACTCACCGCCGCCGATTGCCGATGAGATCCTTTATgcggcg                                                                                                               >  2:584916/1‑150 (MQ=255)
                                             ttCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCt                                                                                                          >  2:2380386/1‑150 (MQ=255)
                                             ttCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCt                                                                                                          >  1:1349079/1‑150 (MQ=255)
                                             ttCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCt                                                                                                          >  1:1411311/1‑150 (MQ=255)
                                             ttCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCt                                                                                                          >  1:33432/1‑150 (MQ=255)
                                             tacAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTATCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATAGCCGATGAGATCCTTTATGCGGCGCAGCt                                                                                                          >  1:1730834/3‑150 (MQ=255)
                                               cAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCtgt                                                                                                        <  1:2439590/150‑1 (MQ=255)
                                                    gggTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGgt                                                                                                   <  2:2541958/150‑1 (MQ=255)
                                                    gggTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGgt                                                                                                   <  2:92065/150‑1 (MQ=255)
                                                       ttGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGCGGAGATCCTTTATGCGGCGCAGCTGTGCGGtgtg                                                                                                >  1:1206858/1‑150 (MQ=255)
                                                       ttGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGAGGAGATCCTTTATGCGGCGCAGCTGTGCGGtgtg                                                                                                >  1:260417/1‑150 (MQ=255)
                                                       ttGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGAGGAGATCCTTTATGCGGCGCAGCTGTGCGGtgtg                                                                                                >  1:2737161/1‑150 (MQ=255)
                                                         gTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCa                                                                                              <  2:2208941/150‑1 (MQ=255)
                                                           atatTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAgg                                                                                            <  2:295776/150‑1 (MQ=255)
                                                            tatTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGa                                                                                           <  2:230863/150‑1 (MQ=255)
                                                            tatTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGa                                                                                           <  2:371978/150‑1 (MQ=255)
                                                            tatTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGa                                                                                           <  2:973873/150‑1 (MQ=255)
                                                            tagtCCTGGCGGCTCCGCCCCGCTCGTCTCAACGGTCTTACTGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGa                                                                                           <  2:243081/147‑1 (MQ=255)
                                                                   ggcggcTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGttt                                                                                    >  2:1521917/1‑150 (MQ=255)
                                                                   ggcggcTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGttt                                                                                    >  2:1624155/1‑150 (MQ=255)
                                                                    gcggcTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTa                                                                                   >  2:1040007/1‑150 (MQ=255)
                                                                    gcggcTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTa                                                                                   >  2:2251918/1‑150 (MQ=255)
                                                                    gcggcTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTa                                                                                   >  2:1039778/1‑150 (MQ=255)
                                                                      ggcTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGgt                                                                                                   <  1:1988133/132‑1 (MQ=255)
                                                                              cccGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTcggcgg                                                                         >  1:463957/1‑150 (MQ=255)
                                                                              cccGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTcggcgg                                                                         >  1:1074756/1‑150 (MQ=255)
                                                                              cccGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTcggcgg                                                                         >  1:1964470/1‑150 (MQ=255)
                                                                              cccGCTCTTCTCAACGGAATTAATGCTGGCGACTCCGGCGATAATAGCGGGCTGTAAAAAAGTGCTGCTGTGCTCACCGCCGCCGAATGCCGAGGAGATCCTTTATGCGGCGCAGCTCTGCGGTGTGCAGGACGTGTTTAACGTcggcgg                                                                         >  1:1469947/1‑150 (MQ=255)
                                                                                     ttctCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAg                                                                  <  2:1994078/150‑1 (MQ=255)
                                                                                      tctcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAgg                                                                 <  1:704888/150‑1 (MQ=255)
                                                                                       ctcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGc                                                                <  1:2579865/150‑1 (MQ=255)
                                                                                       ctcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGc                                                                <  2:1894802/150‑1 (MQ=255)
                                                                                        tcAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGcc                                                               >  2:169198/1‑150 (MQ=255)
                                                                                                 ttAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCa                                                      <  2:146143/150‑1 (MQ=255)
                                                                                                 ttAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCa                                                      <  2:708595/150‑1 (MQ=255)
                                                                                                     tGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTgg                                                  >  1:1547787/1‑150 (MQ=255)
                                                                                                     tGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTgg                                                  >  2:2072089/1‑150 (MQ=255)
                                                                                                       cTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCg                                                <  1:1913497/150‑1 (MQ=255)
                                                                                                          gCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGttt                                             <  1:310708/150‑1 (MQ=255)
                                                                                                          gCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGttt                                             >  2:1280188/1‑150 (MQ=255)
                                                                                                            gACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTgg                                           <  2:2673252/150‑1 (MQ=255)
                                                                                                            gACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTgg                                           <  1:2424162/150‑1 (MQ=255)
                                                                                                            gACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTgg                                           <  2:2745707/150‑1 (MQ=255)
                                                                                                            gACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTgg                                           <  1:551644/150‑1 (MQ=255)
                                                                                                            gACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTgg                                           <  1:1858183/150‑1 (MQ=255)
                                                                                                               tCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTAc                                        >  1:2097417/1‑150 (MQ=255)
                                                                                                               tCCGGCGAGTATTGCGGGCTGCAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTAc                                        >  1:1097880/1‑150 (MQ=255)
                                                                                                                ccGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACg                                       <  1:1510159/150‑1 (MQ=255)
                                                                                                                  ggCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGa                                     >  1:2614090/1‑150 (MQ=255)
                                                                                                                  ggCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGa                                     >  2:2549764/1‑150 (MQ=255)
                                                                                                                        tATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCtgt                               <  1:1227760/150‑1 (MQ=255)
                                                                                                                        tATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCtgt                               <  1:918931/150‑1 (MQ=255)
                                                                                                                          ttGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGc                             <  2:1378889/150‑1 (MQ=255)
                                                                                                                             cGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCaa                          >  2:1738330/1‑150 (MQ=255)
                                                                                                                               ggCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCaaaa                        >  1:326835/1‑150 (MQ=255)
                                                                                                                                gCTGTAAAAAAGTGGTGCTGTACTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAg                       <  1:1037248/150‑1 (MQ=255)
                                                                                                                                gCTGTAAAAAAGTGGTGCTGTACTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAg                       <  1:1671833/150‑1 (MQ=255)
                                                                                                                                gCTGTAAAAAAGTGGTGCTGTACTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAg                       <  1:584916/150‑1 (MQ=255)
                                                                                                                                    tAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGa                   <  2:1209362/150‑1 (MQ=255)
                                                                                                                                    tAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGa                   <  1:246847/150‑1 (MQ=255)
                                                                                                                                    tAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGa                   <  2:1988105/150‑1 (MQ=255)
                                                                                                                                        aaaGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACaaa               <  2:2392920/150‑1 (MQ=255)
                                                                                                                                        aaaGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACaaa               <  2:1968316/150‑1 (MQ=255)
                                                                                                                                        aaaGTGGTGCGGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACaaa               <  2:542641/150‑1 (MQ=255)
                                                                                                                                           gtggtgCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATc            >  2:372248/1‑150 (MQ=255)
                                                                                                                                           gtggtgCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATc            >  2:2750/1‑150 (MQ=255)
                                                                                                                                              gtgctgtgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTc         <  2:1775359/150‑1 (MQ=255)
                                                                                                                                               tgctgtgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCg        >  1:1738166/1‑150 (MQ=255)
                                                                                                                                               tgctgtgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCg        >  1:196671/1‑150 (MQ=255)
                                                                                                                                               tgctgtgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCg        >  1:1931525/1‑150 (MQ=255)
                                                                                                                                               tgctgtgctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCg        >  1:1573356/1‑150 (MQ=255)
                                                                                                                                                     gctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTTTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCgg  <  2:468674/150‑1 (MQ=255)
                                                                                                                                                     gctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAACATCTTCGGGCCgg  <  2:2283929/150‑1 (MQ=255)
                                                                                                                                                     gctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCgg  <  2:2117705/150‑1 (MQ=255)
                                                                                                                                                     gctCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCgg  <  2:661143/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACGCAGCCAGGCGTGCGTTGCCAGCAGGTCACGCGTCCGGTAGCTTCAGTTGGGTTGTATATTCCTGGCGGCTCCGCCCCGCTCTTCTCAACGGTATTAATGCTGGCGACTCCGGCGAGTATTGCGGGCTGTAAAAAAGTGGTGCTGTGCTCACCGCCGCCGATTGCCGATGAGATCCTTTATGCGGCGCAGCTGTGCGGTGTGCAGGACGTGTTTAACGTCGGCGGCGCACAGGCCATTGCCGCACTGGCGTTTGGTACGGAATCTGTGCCAAAAGTGGACAAAATCTTCGGGCCGG  >  NC_000913/2091426‑2091724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: