Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 13.0% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA13.0% 320.5 / 4.2 133intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (51/57);  new base A (15/8);  total (68/65)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383
                                                                                                                                                    |                                                                                                                                                      
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                       <  1:2207193/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                      <  2:1037070/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTaaa                                                                                                                                                      >  1:2532531/1‑150 (MQ=255)
  ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                     <  2:2172111/150‑1 (MQ=255)
  ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                     <  2:2172093/150‑1 (MQ=255)
   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                    <  1:1067880/150‑1 (MQ=255)
   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                    <  1:573810/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                                >  2:2300438/1‑150 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                                >  1:1797161/1‑150 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                                >  2:1436652/1‑150 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTGCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTAGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGAGTTAAAAataaa                                                                                                                                                >  2:2117559/1‑148 (MQ=255)
         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATGTCAAAATATAAt                                                                                                                                              >  2:1139163/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAat                                                                                                                                         >  2:784008/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGAAat                                                                                                                                         >  2:2336297/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                         >  2:2243794/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                         >  2:2746640/1‑150 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATGTCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                      >  2:2167786/1‑150 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                      >  2:1116250/1‑150 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAattat                                                                                                                                      >  2:2438161/1‑150 (MQ=255)
                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                <  1:38917/150‑1 (MQ=255)
                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                >  2:2251865/1‑150 (MQ=255)
                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATGAAATTATtttttt                                                                                                                                >  2:2703069/1‑150 (MQ=255)
                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCTCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATGAAAATATttttttttta                                                                                                                            >  1:2594268/1‑148 (MQ=255)
                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                            >  1:175416/1‑150 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                           <  2:1184193/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                        <  1:39305/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAga                                                                                                                     >  2:367953/1‑150 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATATTTTTTTTTAAATATagag                                                                                                                    >  1:1687968/1‑150 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGTATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                            <  2:1154313/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                            <  2:2165749/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                            <  1:1914489/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTATATATAGAGATAACGAt                                                                                                            >  1:2173615/1‑150 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                          <  1:2031702/150‑1 (MQ=255)
                                              tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAAAAAAATGAAAATATTTGTTTTTAAATATCGAGATAAAGATcaa                                                                                                         >  1:1624657/1‑149 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGTTAAAGATCACAAAAAAGAc                                                                                                >  1:881971/1‑150 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGTAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACa                                                                                               >  1:408645/1‑150 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATAAAATTATAATAATTGTTTTTGAATATAGAGTTAACGATCACACAAACGAACAAATGACAATTATTc                                                                                >  1:1239777/1‑150 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATATTTGTTTTTTAAAATAGGGGTAACGATAACAAAAAAGAAAAAATGAAAattatta                                                                                >  1:1696907/1‑149 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAAGAAAATATTTTTTTTTTAAAATAGAGATAAAGATCACAAAAACGACAAGATGAAAattattg                                                                                >  1:1051417/1‑149 (MQ=255)
                                                                         cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                              <  2:44180/150‑1 (MQ=255)
                                                                         cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                              <  2:1967916/150‑1 (MQ=255)
                                                                         cgcgCTGTGCGTTGTACAGCATATGCTTTGTCTATACCACGTACATTTACATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                              <  2:2368781/150‑1 (MQ=255)
                                                                           cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTTAAAATAGAGATAAAGAACACAAAAACGAAAAAAAGAAAATTATTCtagg                                                                            >  2:517044/1‑150 (MQ=255)
                                                                           cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAATAATGAAAATATTTGTTTTTAAATATAGAGATAACGATAACAAAAAAGACAATATTAAAATTATTAgagg                                                                            >  2:577810/1‑150 (MQ=255)
                                                                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                    <  2:617493/150‑1 (MQ=255)
                                                                                   gtggttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                    <  2:629644/147‑1 (MQ=255)
                                                                                       ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAAAATAAAGAAATTATTTGTTTTTAAAGATCGAGATAAAGAACACAAAAACGACAATATGTAACATATTAGAGGAGTGAAAGGcca                                                                >  2:730341/1‑148 (MQ=255)
                                                                                        tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:2628019/150‑1 (MQ=255)
                                                                                        tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:1057912/150‑1 (MQ=255)
                                                                                        tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:2475310/150‑1 (MQ=255)
                                                                                        tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:1373439/150‑1 (MQ=255)
                                                                                        tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:1830429/150‑1 (MQ=255)
                                                                                         cAGCATCTGCTTTTTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                             <  2:1532166/150‑1 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                             <  2:259906/150‑1 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATTTAATTAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                             <  2:259682/150‑1 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTACATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                             <  2:4675/150‑1 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGAGACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATTTCTAGATAAGGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGTAAGGCaaaa                                                             <  2:1532213/150‑1 (MQ=255)
                                                                                         cAGCAACTGATTTGTCGAAACCACCTACATTTTCATAACCTGTCTTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                             <  2:2320134/150‑1 (MQ=255)
                                                                                            attCTGTTTTGTCGATACCACGAACTTTTTCATACCCTGTTGTTTGTTTTCTTTTTAAAAATATAATGAATTTTTTTTTTTTTAATTATCGAGATAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  1:1009222/148‑1 (MQ=255)
                                                                                                tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATTTCGAGATAACGATCACAAAAACGACACTATGAAAATTATTCGCGGAGGGGAAGGCAAAAAAACGGc                                                      >  2:2275427/1‑150 (MQ=255)
                                                                                                 gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGCGATAACGATCCCAAAAACCCCAAAATGAAAAATATTCGCGGCGTGAAAGGCACAAAAACGGcc                                                     >  2:123917/1‑150 (MQ=255)
                                                                                                 gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGcc                                                     >  2:1813020/1‑150 (MQ=255)
                                                                                                 gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGAAAATTATTCGGGGAGTGAAAGGCAAAAAAACGGcc                                                     >  2:1604853/1‑150 (MQ=255)
                                                                                                              cACGAACATTTTCATAGCCTGTCTTTTTTTTTCTATTAAAAAATATAATTAATTTATTTTTTTTAAAATTTCGATAAAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:2370970/150‑1 (MQ=255)
                                                                                                                  tACATTTGCATCTCCTGTGGTTTGTTTTCGTTTACAAAATATAATGAATTTTTTTGTTTTTAAATATCGAGATAACGACCACAACCACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                    <  1:561132/150‑1 (MQ=255)
                                                                                                                    cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  2:1687968/150‑1 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAAAGAAATTAATTGTTTTTAAAAATCGCGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGGGTGAAAGGCAAAAAAAAGGCCACCCGCTAGGGAAGcccgag                                 >  2:1859091/1‑146 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAAAGAAAATATTTTTTTTTAAAAATAGAGATAAAGAACACAAAAAACACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCCCTAGGGAAGCCGTAg                                 >  2:1314188/1‑150 (MQ=255)
                                                                                                                         tCATCTCGTGGCGTTTGTTTTCGATTAAAAAATATATTTAAATTTTTTTTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  1:1222430/150‑1 (MQ=255)
                                                                                                                         tCATCTCCGGTCGTTTGTTTTCGATTTCAAATTATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAACCTACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCata                             <  1:2012173/150‑3 (MQ=255)
                                                                                                                         tCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  1:1737273/150‑1 (MQ=255)
                                                                                                                         ccATAGCCTGTCATTGGTTTTCGATTACAAAATTTAATTAAATTTTTTGTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  1:128939/149‑1 (MQ=255)
                                                                                                                          cATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCAAAg                            >  2:2021544/1‑150 (MQ=255)
                                                                                                                             atCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                         <  2:705168/150‑1 (MQ=255)
                                                                                                                                cTGTCGTTTGTTTTCTATTTCAAAATATTATGATATTATTTGTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                      <  2:559584/150‑1 (MQ=255)
                                                                                                                                cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                      <  2:1968883/150‑1 (MQ=255)
                                                                                                                                cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                      <  2:559591/150‑1 (MQ=255)
                                                                                                                                 tttCGTTTTTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  2:1181228/148‑1 (MQ=255)
                                                                                                                                 tgttgtTTTTTTTCGTTTTAAAAATTTTATGAAATTTTTTTTTTTTAAATTTGGAGCTAACGATCACAAAAACGCCAATTTTTAAATTTTTCGCTGTGTGTAATGCAAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                      <  2:1515362/150‑1 (MQ=255)
                                                                                                                                 tGTCTTTTGTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTTATTTTATAGATAAATATCAAAAAAACGACAATATGAAATTTATTGGAGGAGTGAAAGGCAAAAAAACGGCCTCGCGATAGGGAAGCCGTTGCAAAGTGCGCgt                     <  2:1243353/150‑1 (MQ=255)
                                                                                                                                 tGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  2:1181246/150‑1 (MQ=255)
                                                                                                                                 ggTCGTTTGTTTTCGATTTAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACTACCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  2:2386523/149‑1 (MQ=255)
                                                                                                                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTATCAAAGTGCGCgtg                    >  2:1867780/1‑150 (MQ=255)
                                                                                                                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                    >  2:1093361/1‑150 (MQ=255)
                                                                                                                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                    >  2:2753530/1‑150 (MQ=255)
                                                                                                                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                    >  2:323716/1‑150 (MQ=255)
                                                                                                                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACCGACTCCCGATAGGGACGCCGTAGCACAGTGcgcggg                    >  2:1443021/1‑148 (MQ=255)
                                                                                                                                  gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcggg                    >  2:1527651/1‑148 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                >  2:839385/1‑150 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                >  2:3704/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAATGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:83009/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:2011940/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:2753575/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:172419/1‑150 (MQ=255)
                                                                                                                                            ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGGGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          >  2:1678049/1‑150 (MQ=255)
                                                                                                                                             tagATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  1:2554586/148‑1 (MQ=255)
                                                                                                                                                 tttCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     <  1:2159247/150‑1 (MQ=255)
                                                                                                                                                   tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   <  1:646564/150‑1 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:667196/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:246408/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: