Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,358,643 G→A 6.4% A68V (GCC→GTC)  rhmA ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,358,6430GA 6.4% 530.5 / 17.3 155A68V (GCC→GTC) rhmA2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (74/71);  new base A (5/5);  total (79/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 162 total aligned reads displayed.

CGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACCACAGACCAATTT  >  NC_000913/2358494‑2358790
                                                                                                                                                     |                                                                                                                                                   
cGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTtggctgg                                                                                                                                                     <  2:2587794/150‑1 (MQ=255)
cGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTtggctgg                                                                                                                                                     <  2:2135832/150‑1 (MQ=255)
          aTAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgcgc                                                                                                                                           >  1:906467/1‑150 (MQ=255)
          aTAGCGCGTGGCAGACACCACCTGACGAGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTATGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGAACACGGGTTGGCTGGCATAGGgcgc                                                                                                                                           >  1:906473/1‑150 (MQ=255)
            aGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTa                                                                                                                                         <  2:1859588/150‑1 (MQ=255)
            aGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTa                                                                                                                                         <  2:1542559/150‑1 (MQ=255)
                          accaccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCCACCGCCTGTAGCtga                                                                                                                           >  1:2695847/1‑150 (MQ=255)
                          accaccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCCACCGCCTGTAGCtga                                                                                                                           >  1:1676923/1‑150 (MQ=255)
                            caccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGACATAGGGCGCTACCGCCTGTAGCtgatg                                                                                                                         >  2:2673385/1‑150 (MQ=255)
                            caccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGACATAGGGCGCTACCGCCTGTAGCtgatg                                                                                                                         >  2:208434/1‑150 (MQ=255)
                            caccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGACATAGGGCGCTACCGCCTGTAGCtgatg                                                                                                                         >  2:1714238/1‑150 (MQ=255)
                              ccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGATTTTGCGCGCCAAAATCCACGACTTGTTTAATCATCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCAACCGCCTGTAGCTgatgat                                                                                                                       >  1:2245502/1‑150 (MQ=255)
                              ccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTgatgat                                                                                                                       >  1:795210/1‑150 (MQ=255)
                                tGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaa                                                                                                                     <  2:1155361/150‑1 (MQ=255)
                                tGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaa                                                                                                                     <  2:111487/150‑1 (MQ=255)
                                tGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaa                                                                                                                     <  2:284599/150‑1 (MQ=255)
                                      gCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTCCCGCCTGTAGCTGATGATAAAGATcc                                                                                                               >  2:286719/1‑150 (MQ=255)
                                      gCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATcc                                                                                                               <  1:1657549/150‑1 (MQ=255)
                                       ccTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCt                                                                                                              <  1:1802943/150‑1 (MQ=255)
                                         tGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGa                                                                                                            >  2:785365/1‑150 (MQ=255)
                                         tGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGa                                                                                                            >  2:2162256/1‑150 (MQ=255)
                                          gTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGaa                                                                                                           <  2:1343622/150‑1 (MQ=255)
                                             cGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATgg                                                                                                        >  2:2489055/1‑150 (MQ=255)
                                              ggCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATCTCCAGGACTTGTTTAATCAGCGGTGTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGgt                                                                                                       <  2:1461325/150‑1 (MQ=255)
                                              ggCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGgt                                                                                                       <  2:1080421/150‑1 (MQ=255)
                                              ggCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGgt                                                                                                       <  2:649200/150‑1 (MQ=255)
                                                 aGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGtt                                                                                                    >  1:2120959/1‑150 (MQ=255)
                                                       gACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGgcgc                                                                                              >  2:897176/1‑150 (MQ=255)
                                                       gACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGgcgc                                                                                              <  2:820856/150‑1 (MQ=255)
                                                       gACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGgcgc                                                                                              <  2:523270/150‑1 (MQ=255)
                                                          cATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTg                                                                                           <  1:2091064/150‑1 (MQ=255)
                                                            tCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCt                                                                                         >  2:2673448/1‑150 (MQ=255)
                                                            tCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCt                                                                                         >  2:104410/1‑150 (MQ=255)
                                                            tCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCt                                                                                         >  2:1874622/1‑150 (MQ=255)
                                                             cGGGATCAGTAGAGTGTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCAGAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTc                                                                                        <  1:505461/150‑1 (MQ=255)
                                                                 aTCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCg                                                                                    <  1:1806014/150‑1 (MQ=255)
                                                                      tAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAAt                                                                               >  1:1178294/1‑150 (MQ=255)
                                                                                gcgcCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAg                                                                     <  1:1840746/150‑1 (MQ=255)
                                                                                gcgcCAATATCCAGGACTCGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAg                                                                     <  1:1863661/150‑1 (MQ=255)
                                                                                    cAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCAt                                                                 >  2:1062249/1‑150 (MQ=255)
                                                                                       tatCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGACATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAAc                                                              <  1:2673450/150‑1 (MQ=255)
                                                                                       tatCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGACATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAAc                                                              <  1:208238/150‑1 (MQ=255)
                                                                                       tatCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGACATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAAc                                                              <  1:2673385/150‑1 (MQ=255)
                                                                                         tCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCa                                                            <  1:692168/150‑1 (MQ=255)
                                                                                         tCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCa                                                            <  1:142937/150‑1 (MQ=255)
                                                                                         tCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCa                                                            <  2:1335156/150‑1 (MQ=255)
                                                                                          ccAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAg                                                           >  2:466915/1‑150 (MQ=255)
                                                                                           cAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGa                                                          >  2:83012/1‑150 (MQ=255)
                                                                                           cAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGa                                                          <  2:2009584/150‑1 (MQ=255)
                                                                                           cAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGa                                                          >  1:2015485/1‑150 (MQ=255)
                                                                                             ggACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAg                                                        >  2:2506620/1‑150 (MQ=255)
                                                                                              gACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGt                                                       <  1:1731717/150‑1 (MQ=255)
                                                                                              gACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGt                                                       <  2:1215616/150‑1 (MQ=255)
                                                                                                cTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTgg                                                     <  1:624279/150‑1 (MQ=255)
                                                                                                cTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTgg                                                     <  1:690059/150‑1 (MQ=255)
                                                                                                   gTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTggcgg                                                  <  1:2255251/150‑1 (MQ=255)
                                                                                                   gTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTggcgg                                                  <  1:372959/150‑1 (MQ=255)
                                                                                                   gTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTggcgg                                                  <  1:1923638/150‑1 (MQ=255)
                                                                                                    tttAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAAACAGCAACCAGTCATAACCAGAAGTggcggc                                                 >  2:1187219/1‑150 (MQ=255)
                                                                                                           aGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCt                                          >  2:1265820/1‑150 (MQ=255)
                                                                                                             cGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGc                                        <  2:612715/150‑1 (MQ=255)
                                                                                                             cGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGc                                        <  1:1105772/150‑1 (MQ=255)
                                                                                                             cGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGc                                        >  2:2363424/1‑150 (MQ=255)
                                                                                                             cGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGc                                        <  1:1239431/150‑1 (MQ=255)
                                                                                                             cGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGc                                        <  1:2063099/150‑1 (MQ=255)
                                                                                                             cGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGc                                        >  1:2750410/1‑150 (MQ=255)
                                                                                                             cGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGc                                        <  1:422466/150‑1 (MQ=255)
                                                                                                                    cTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAg                                 <  1:790965/150‑1 (MQ=255)
                                                                                                                    cTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAg                                 <  1:235563/150‑1 (MQ=255)
                                                                                                                    cTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAg                                 <  1:235601/150‑1 (MQ=255)
                                                                                                                    cTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAg                                 <  1:1517088/150‑1 (MQ=255)
                                                                                                                    cTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAg                                 <  1:438385/150‑1 (MQ=255)
                                                                                                                     tGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTCGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCAGATAgg                                >  2:2674561/1‑150 (MQ=255)
                                                                                                                     tGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGGTTGGCGCGTGCTCCCCGTCAATCAGCACCCCGGCATACCCAGACGGGGCGGCAATGGCTGCCATATCgg                                >  2:2674569/1‑150 (MQ=255)
                                                                                                                         ttCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGt                            >  1:781532/1‑150 (MQ=255)
                                                                                                                               cggacggaTCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGa                      <  1:466413/150‑1 (MQ=255)
                                                                                                                               cggacggaTCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGa                      <  2:2648968/150‑1 (MQ=255)
                                                                                                                                    ggaTCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTa                 >  2:1873325/1‑150 (MQ=255)
                                                                                                                                    ggaTCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTa                 >  2:2329463/1‑150 (MQ=255)
                                                                                                                                    ggaTCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTa                 >  2:140519/1‑150 (MQ=255)
                                                                                                                                     gaTCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTaa                <  1:1275978/150‑1 (MQ=255)
                                                                                                                                      aTCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAAc               >  1:2640546/1‑150 (MQ=255)
                                                                                                                                        cACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACca             <  1:1265820/150‑1 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:1357113/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:2148617/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:936794/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:2179956/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:1073990/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:2290110/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:2459482/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:2619399/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:2625570/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:370322/1‑150 (MQ=255)
                                                                                                                                         aCGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:556889/1‑150 (MQ=255)
                                                                                                                                         aCGGGATGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACcac            >  1:1677802/1‑150 (MQ=255)
                                                                                                                                            ggTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACCACAGa         <  2:444931/150‑1 (MQ=255)
                                                                                                                                            ggTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACCACAGa         <  2:1592398/150‑1 (MQ=255)
                                                                                                                                            ggTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACCACAGa         <  2:1592391/150‑1 (MQ=255)
                                                                                                                                             gTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACCACAGAc        <  1:301518/150‑1 (MQ=255)
                                                                                                                                                 gctggcATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACCACAGACCAAt    >  2:2725148/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAAGTGGCGGCAATTTCTGCCATATAGGCAGTCGTTGAGCTTAACCACAGACCAATTT  >  NC_000913/2358494‑2358790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: