Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,788,673 A→T 8.3% intergenic (+24/‑312) ygaQ → / → csiD uncharacterized protein; putative enzyme/carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,788,6730AT 8.3% 455.3 / 25.8 133intergenic (+24/‑312)ygaQ/csiDuncharacterized protein; putative enzyme/carbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (67/55);  new base T (6/5);  total (73/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 139 total aligned reads displayed.

CGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTT  >  NC_000913/2788524‑2788822
                                                                                                                                                     |                                                                                                                                                     
cGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATa                                                                                                                                                       >  2:587823/1‑150 (MQ=255)
 gAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATaa                                                                                                                                                      <  1:1183999/150‑1 (MQ=255)
       ttATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACggg                                                                                                                                                >  2:133182/1‑150 (MQ=255)
       ttATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACggg                                                                                                                                                >  1:2255019/1‑150 (MQ=255)
       ttATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACggg                                                                                                                                                <  1:2725046/150‑1 (MQ=255)
       ttATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACggg                                                                                                                                                <  1:2686166/150‑1 (MQ=255)
        tATCCACCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTATGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACgggg                                                                                                                                               <  2:1334360/150‑1 (MQ=255)
         aTCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGc                                                                                                                                              <  1:1703254/150‑1 (MQ=255)
              cccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGata                                                                                                                                         <  1:335572/150‑1 (MQ=255)
              cccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGata                                                                                                                                         <  2:2130350/150‑1 (MQ=255)
                 tGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGataata                                                                                                                                      >  1:1469712/1‑150 (MQ=255)
                 tGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGataata                                                                                                                                      >  1:2380930/1‑150 (MQ=255)
                  ggTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAatat                                                                                                                                     <  1:715027/150‑1 (MQ=255)
                      aaGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgttt                                                                                                                                 >  2:1845426/1‑150 (MQ=255)
                        gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTCTCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                               >  1:2482292/1‑150 (MQ=255)
                        gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                               >  1:1682876/1‑150 (MQ=255)
                        gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                               >  1:2159412/1‑150 (MQ=255)
                        gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                               >  1:1886670/1‑150 (MQ=255)
                        gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttag                                                                                                                               >  1:1949302/1‑150 (MQ=255)
                            ttACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtttagttta                                                                                                                           <  2:349384/150‑1 (MQ=255)
                             tACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTAc                                                                                                                          <  1:1046883/150‑1 (MQ=255)
                               cGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTCTCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTa                                                                                                                        >  1:818277/1‑150 (MQ=255)
                               cGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTCTCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTa                                                                                                                        >  1:1140849/1‑150 (MQ=255)
                                 cTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTAATTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTaaa                                                                                                                      <  2:788292/150‑2 (MQ=255)
                                      agGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCa                                                                                                                 <  1:587823/150‑1 (MQ=255)
                                             aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                          <  2:1737048/150‑1 (MQ=255)
                                             aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                          <  2:1736999/150‑1 (MQ=255)
                                             aCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCa                                                                                                          <  2:2514229/150‑1 (MQ=255)
                                                ctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTg                                                                                                       >  2:1437448/1‑150 (MQ=255)
                                                  aaaTATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGaa                                                                                                     <  2:1012031/150‑1 (MQ=255)
                                                  aaaTATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGaa                                                                                                     <  2:540182/150‑1 (MQ=255)
                                                    atatCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGc                                                                                                   <  2:545528/150‑1 (MQ=255)
                                                           tatTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTAc                                                                                            <  2:1778906/150‑1 (MQ=255)
                                                                  gggCGTAGCGCTCTTCTTTTAGGGAAAACGGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAGTTACTGttttt                                                                                     >  1:869799/1‑150 (MQ=255)
                                                                  gggCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttttt                                                                                     >  1:393473/1‑150 (MQ=255)
                                                                  gggCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttttt                                                                                     >  1:869800/1‑150 (MQ=255)
                                                                  gggCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTGATAATTTAATGTTATATATGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGACATTTACTGttttt                                                                                     >  1:1220171/1‑150 (MQ=255)
                                                                    gCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTa                                                                                   <  2:2232817/150‑1 (MQ=255)
                                                                      gTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATc                                                                                 <  2:2033260/150‑1 (MQ=255)
                                                                            ctctTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAg                                                                           >  1:2520393/1‑150 (MQ=255)
                                                                               ttcttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATa                                                                        >  2:1391868/1‑150 (MQ=255)
                                                                                 cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                      <  1:2595640/150‑1 (MQ=255)
                                                                                   tgtAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCGGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCt                                                                    <  1:1259805/148‑1 (MQ=255)
                                                                                    ttAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCtt                                                                   <  2:372346/150‑2 (MQ=255)
                                                                                      aGGGAAAACAGGAGAACCGCCGAACTATCTCTATTGATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGtt                                                                 <  1:1909363/150‑1 (MQ=255)
                                                                                             aCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAAt                                                          <  2:1186319/150‑1 (MQ=255)
                                                                                               aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAAt                                                          <  1:1711857/148‑1 (MQ=255)
                                                                                                ggAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGtt                                                       >  1:751060/1‑150 (MQ=255)
                                                                                                ggAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGtt                                                       >  2:2187810/1‑150 (MQ=255)
                                                                                                ggAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGACCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAATCCATTTACTTTTTTTTATCGAACAGATAATCTGTTCTCTAATGtt                                                       >  2:1662574/1‑150 (MQ=255)
                                                                                                  agaACCGCCGAACTATCTCTATTTATAATTTACTGTTATATCTTCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTTCAGTTAAGCCATTTACTGTTTTTTATAGACCAGATAATCTGTTCTCTAATGTTaa                                                     <  1:1239367/150‑1 (MQ=255)
                                                                                                  agaACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTaa                                                     <  1:478452/150‑1 (MQ=255)
                                                                                                  agaACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTaa                                                     <  1:342846/150‑1 (MQ=255)
                                                                                                  agaACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTaa                                                     <  1:2083394/150‑1 (MQ=255)
                                                                                                   gaACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAAc                                                    <  2:2020857/150‑1 (MQ=255)
                                                                                                    aaCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGTGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGAAGTGAAGCCATTTACTGTTTTTTATCTACAAGATAATCTGTTCTATAATGTTAACt                                                   >  2:1888331/1‑150 (MQ=255)
                                                                                                    aaCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACt                                                   <  1:1699406/150‑1 (MQ=255)
                                                                                                    aaCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACt                                                   >  2:2241256/1‑150 (MQ=255)
                                                                                                    aaCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTATATTTTTAACt                                                   >  2:944285/1‑150 (MQ=255)
                                                                                                    aaCCGCCGAACTAACTCTATTTATAATTAAATGTTATATCTGACCCGATAAAACGGGGCAGATAATATGATTAGTATACTAACGGACATTTTTCAGTGAAGCCATATACAGTTTTTTATCGACCAGAAAATCTGTTCTCTAATGTTAACt                                                   >  2:180718/1‑150 (MQ=255)
                                                                                                     aCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTc                                                  <  2:468787/150‑1 (MQ=255)
                                                                                                               tATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCt                                        <  2:986334/150‑1 (MQ=255)
                                                                                                                   tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTg                                    <  2:1469712/150‑1 (MQ=255)
                                                                                                                   tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTg                                    <  2:2690157/150‑1 (MQ=255)
                                                                                                                   tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTg                                    <  2:939169/150‑1 (MQ=255)
                                                                                                                    ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                   >  2:1494619/1‑150 (MQ=255)
                                                                                                                    ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                   >  2:497967/1‑150 (MQ=255)
                                                                                                                    ctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGc                                   >  1:564837/1‑150 (MQ=255)
                                                                                                                     tATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCt                                  <  1:1169828/150‑1 (MQ=255)
                                                                                                                     tATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCt                                  <  1:2601962/150‑1 (MQ=255)
                                                                                                                       tttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCttt                                >  1:1709981/1‑150 (MQ=255)
                                                                                                                       tttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCttt                                >  1:2203138/1‑150 (MQ=255)
                                                                                                                       tttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCttt                                >  2:893255/1‑150 (MQ=255)
                                                                                                                       tttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCAAATGTTAACTCCCCCTAACCTGTTGCttt                                >  2:1681873/1‑150 (MQ=255)
                                                                                                                         tataATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAg                              >  2:1355446/1‑150 (MQ=255)
                                                                                                                          ataATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGAGAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGt                             >  2:2323399/1‑150 (MQ=255)
                                                                                                                            aaTTTAATGTTATATCTGCCCAGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAATTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTa                           >  2:1022180/1‑150 (MQ=255)
                                                                                                                             aTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAt                          <  1:677002/150‑1 (MQ=255)
                                                                                                                             aTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAt                          <  1:1391868/150‑1 (MQ=255)
                                                                                                                             aTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAt                          <  2:1468849/150‑1 (MQ=255)
                                                                                                                              tttAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAtt                         >  1:443139/1‑150 (MQ=255)
                                                                                                                              tttAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAtt                         >  1:442734/1‑150 (MQ=255)
                                                                                                                              tttAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAtt                         >  2:220008/1‑150 (MQ=255)
                                                                                                                              tttAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAtt                         >  2:1229029/1‑150 (MQ=255)
                                                                                                                               ttAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTc                        <  2:2332898/150‑1 (MQ=255)
                                                                                                                               ttAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTc                        <  2:2336676/150‑1 (MQ=255)
                                                                                                                                  acatattatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGGTATTCTGTTCTCTATTGTTAACTCCCCCTAACGTGTTGCTTTAGTTATTAAtt                     >  1:1921128/7‑150 (MQ=255)
                                                                                                                                  acatattatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAtt                     >  1:700801/7‑150 (MQ=255)
                                                                                                                                  acatattatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAtt                     >  1:1987012/7‑150 (MQ=255)
                                                                                                                                  acatattatCTGCCCCGTTTTATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAtt                     >  1:862832/7‑150 (MQ=255)
                                                                                                                                    gTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTc                   >  1:2033263/1‑150 (MQ=255)
                                                                                                                                     ttATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTcc                  <  1:1443278/150‑1 (MQ=255)
                                                                                                                                     ttATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTcc                  >  1:2501932/1‑150 (MQ=255)
                                                                                                                                                ccGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACttt       >  1:2502169/1‑150 (MQ=255)
                                                                                                                                                ccGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACttt       >  1:2708520/1‑150 (MQ=255)
                                                                                                                                                ccGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACttt       >  2:1868120/1‑150 (MQ=255)
                                                                                                                                                  gATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGc     >  1:1140585/1‑150 (MQ=255)
                                                                                                                                                    tAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCt   >  2:1141167/1‑150 (MQ=255)
                                                                                                                                                     aaaaCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCtt  <  2:2482292/150‑1 (MQ=255)
                                                                                                                                                     aaaaCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCtt  <  2:186982/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGAAAAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTT  >  NC_000913/2788524‑2788822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑