Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,822,841 A→G 5.6% L310P (CTG→CCG)  recA ← DNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,822,8410AG 5.6% 475.6 / 13.4 143L310P (CTG→CCG) recADNA recombination and repair protein; ssDNA‑dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP‑dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
Reads supporting (aligned to +/- strand):  ref base A (76/59);  new base G (4/4);  total (80/63)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 150 total aligned reads displayed.

GTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTG  >  NC_000913/2822692‑2822986
                                                                                                                                                     |                                                                                                                                                 
gtATCAAACACGACGATTACACATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCa                                                                                                                                                   <  1:1642410/150‑1 (MQ=255)
gtATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCa                                                                                                                                                   <  1:2299240/150‑1 (MQ=255)
gtATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCa                                                                                                                                                   <  1:1780992/150‑1 (MQ=255)
gtATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCa                                                                                                                                                   <  1:175643/150‑1 (MQ=255)
gtATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCa                                                                                                                                                   <  1:524077/150‑1 (MQ=255)
gtATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCa                                                                                                                                                   <  1:1307100/150‑1 (MQ=255)
gtATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCa                                                                                                                                                   <  2:2104673/150‑1 (MQ=255)
    cAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCa                                                                                                                                               <  2:602251/150‑1 (MQ=255)
           gACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTc                                                                                                                                        <  2:1470311/150‑1 (MQ=255)
               aTTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAt                                                                                                                                    >  2:615529/1‑150 (MQ=255)
               aTTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAt                                                                                                                                    >  2:2609742/1‑150 (MQ=255)
               aTTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAt                                                                                                                                    >  2:2048040/1‑150 (MQ=255)
                ttAACAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCAtt                                                                                                                                   <  2:2171062/150‑1 (MQ=255)
                    aaaTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCGGCCAGGCAGTCGCATTCGCt                                                                                                                               >  2:1797526/1‑150 (MQ=255)
                    aaaTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCGGCCAGGCAGTCGCATTCGCt                                                                                                                               >  2:2498388/1‑150 (MQ=255)
                    aaaTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCGGCCAGGCAGTCGCATTCGCt                                                                                                                               >  1:1287453/1‑150 (MQ=255)
                      aTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt                                                                                                                             >  2:1395564/1‑150 (MQ=255)
                      aTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt                                                                                                                             >  2:2614587/1‑150 (MQ=255)
                      aTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt                                                                                                                             >  2:801285/1‑150 (MQ=255)
                      aTCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCttt                                                                                                                             >  2:913115/1‑150 (MQ=255)
                       tCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                                                                                                                            <  1:1448793/150‑1 (MQ=255)
                       tCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                                                                                                                            <  2:2635926/150‑1 (MQ=255)
                       tCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                                                                                                                            <  2:2713866/150‑1 (MQ=255)
                       tCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCCCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa                                                                                                                            <  1:2479705/150‑1 (MQ=255)
                         ttCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAcc                                                                                                                          <  2:1015981/150‑1 (MQ=255)
                         ttCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAcc                                                                                                                          >  1:1373246/1‑150 (MQ=255)
                         ttCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACACACGTACTTTCTTCTCGATCTCTTTAGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTAcc                                                                                                                          >  1:933388/1‑150 (MQ=255)
                                gTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCg                                                                                                                   <  2:1148279/150‑1 (MQ=255)
                                 tttCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGa                                                                                                                  >  2:2148255/1‑150 (MQ=255)
                                 tttCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGa                                                                                                                  >  1:873509/1‑150 (MQ=255)
                                    cTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtct                                                                                                               <  1:197631/150‑1 (MQ=255)
                                       cTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtcttct                                                                                                            >  2:2572457/1‑150 (MQ=255)
                                         aCGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCa                                                                                                          >  1:1355258/1‑150 (MQ=255)
                                             cTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCtt                                                                                                      >  1:1194185/1‑150 (MQ=255)
                                              ttCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCttt                                                                                                     >  2:889543/1‑150 (MQ=255)
                                              ttCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCttt                                                                                                     <  2:1117923/150‑1 (MQ=255)
                                              ttCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCttt                                                                                                     >  2:1140989/1‑150 (MQ=255)
                                              ttCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCttt                                                                                                     >  2:1146913/1‑150 (MQ=255)
                                              ttCGCGATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCttt                                                                                                     <  2:390772/150‑1 (MQ=255)
                                                    atcatcTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCt                                                                                               <  2:1547662/150‑1 (MQ=255)
                                                    atcatcTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCt                                                                                               <  2:2191934/150‑1 (MQ=255)
                                                    atcatcTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCt                                                                                               <  2:2711272/150‑1 (MQ=255)
                                                          tACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCa                                                                                         >  2:502881/1‑150 (MQ=255)
                                                               agaAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACgcgc                                                                                    >  1:1560386/1‑150 (MQ=255)
                                                                 aaaTCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCt                                                                                  <  2:2097685/150‑1 (MQ=255)
                                                                 aaaTCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCt                                                                                  <  1:1552815/150‑1 (MQ=255)
                                                                 aaaTCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCt                                                                                  <  2:851305/150‑1 (MQ=255)
                                                                 aaaTCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCt                                                                                  <  2:2601142/150‑1 (MQ=255)
                                                                 aaaTCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCt                                                                                  <  1:397160/150‑1 (MQ=255)
                                                                      cGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCttt                                                                             >  2:853716/1‑150 (MQ=255)
                                                                      cGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCttt                                                                             >  1:2549788/1‑150 (MQ=255)
                                                                      cGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCttt                                                                             >  2:2065083/1‑150 (MQ=255)
                                                                      cGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCttt                                                                             >  2:449445/1‑150 (MQ=255)
                                                                      cGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCttt                                                                             >  1:1725974/1‑150 (MQ=255)
                                                                      cGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCttt                                                                             >  2:594624/1‑150 (MQ=255)
                                                                         cGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTtctc                                                                          <  2:1870585/150‑1 (MQ=255)
                                                                           ttGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGa                                                                        <  2:1871326/150‑1 (MQ=255)
                                                                             gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATc                                                                      <  2:1175344/150‑1 (MQ=255)
                                                                             gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCATTGTAGCTGTACCACGCGCCTGCTTTCTCGATc                                                                      <  1:1080380/150‑1 (MQ=255)
                                                                                ttCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAga                                                                   >  2:404042/1‑149 (MQ=255)
                                                                                ttCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGc                                                                   >  1:2167506/1‑150 (MQ=255)
                                                                                ttCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGc                                                                   >  2:372523/1‑150 (MQ=255)
                                                                                ttCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGc                                                                   >  1:2167287/1‑150 (MQ=255)
                                                                                ttCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGc                                                                   >  2:1790987/1‑150 (MQ=255)
                                                                                ttCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGc                                                                   >  2:612140/1‑150 (MQ=255)
                                                                                ttCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGACAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGc                                                                   >  2:351464/1‑150 (MQ=255)
                                                                                     gTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTtctc                                                              <  2:2378067/150‑1 (MQ=255)
                                                                                      gtGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTctct                                                             <  2:2358751/149‑1 (MQ=255)
                                                                                        gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCttt                                                           >  2:1512452/1‑150 (MQ=255)
                                                                                        gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCttt                                                           >  2:2260397/1‑150 (MQ=255)
                                                                                        gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCttt                                                           >  2:1298272/1‑150 (MQ=255)
                                                                                        gCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCACGATCAGCTTCTCttt                                                           >  2:1113231/1‑150 (MQ=255)
                                                                                          tCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTa                                                         <  2:750441/150‑1 (MQ=255)
                                                                                          tCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTa                                                         <  2:345495/150‑1 (MQ=255)
                                                                                           cagcagCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTAc                                                        >  2:2462935/1‑150 (MQ=255)
                                                                                           cagcagCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTAc                                                        >  1:19703/1‑150 (MQ=255)
                                                                                              cagcaACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGcc                                                     >  1:1041900/1‑150 (MQ=255)
                                                                                                     tCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCa                                              >  2:851546/1‑150 (MQ=255)
                                                                                                     tCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCa                                              >  2:276289/1‑150 (MQ=255)
                                                                                                            cTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGtt                                       <  1:705211/150‑1 (MQ=255)
                                                                                                            cTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGtt                                       <  1:1752066/150‑1 (MQ=255)
                                                                                                               tcttctCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGc                                    <  2:2414590/150‑1 (MQ=255)
                                                                                                               tcttctCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGc                                    <  1:2504466/150‑1 (MQ=255)
                                                                                                                cttctCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGcc                                   >  2:760184/1‑150 (MQ=255)
                                                                                                                 ttctCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCg                                  <  1:1217821/150‑1 (MQ=255)
                                                                                                                      gATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGaa                             >  1:244263/1‑150 (MQ=255)
                                                                                                                      gATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGaa                             >  1:796702/1‑150 (MQ=255)
                                                                                                                      gATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGaa                             >  1:1921754/1‑150 (MQ=255)
                                                                                                                      gATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGaa                             >  1:1456969/1‑150 (MQ=255)
                                                                                                                      gATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGaa                             >  1:102245/1‑150 (MQ=255)
                                                                                                                      gATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGACGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGaa                             >  1:2116478/1‑150 (MQ=255)
                                                                                                                        tctcTTTCGCGGTTTCCGGGTTATCTTTCGGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGt                           <  1:1797526/150‑1 (MQ=255)
                                                                                                                        tctcTTTCGCGGTTTCCGGGTTATCTTTCGGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGt                           <  2:1287453/150‑1 (MQ=255)
                                                                                                                          tctTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTg                         >  2:1469306/1‑150 (MQ=255)
                                                                                                                            tttCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGAt                       >  1:1905904/1‑150 (MQ=255)
                                                                                                                                gcgGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCt                   >  1:192048/1‑150 (MQ=255)
                                                                                                                                    tttCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGc               >  1:1032553/1‑150 (MQ=255)
                                                                                                                                         gggTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTag          <  1:2213412/150‑1 (MQ=255)
                                                                                                                                          ggTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCGTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTGACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTaga         <  2:1406359/150‑1 (MQ=255)
                                                                                                                                                 tttCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCGTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTg  >  1:862798/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GTATCAAACAAGACGATTAAAAATCTTCGTTAGTTTCTGCTACGCCTTCGCTATCATCTACAGAGAAATCCGGCGTTGAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTG  >  NC_000913/2822692‑2822986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: