Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913222,9580A.99.1% 1041.3 / 2.2 215coding (126/576 nt)gmhBD,D‑heptose 1,7‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (105/108);  total (105/110)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 240 total aligned reads displayed.

TGCCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCTATT  >  NC_000913/222809‑223103
                                                                                                                                                      |                                                                                                                                                 
tGCCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑a                                                                                                                                                   <  1:2613410/150‑1 (MQ=255)
tGCCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑a                                                                                                                                                   <  1:2258304/150‑1 (MQ=255)
 gCCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aa                                                                                                                                                  <  1:3729740/150‑1 (MQ=255)
   cTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaa                                                                                                                                                <  2:3417287/150‑1 (MQ=255)
   cTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaa                                                                                                                                                <  2:1504098/150‑1 (MQ=255)
     tattaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaaaa                                                                                                                                              >  1:2713108/1‑150 (MQ=255)
     tattaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaaaa                                                                                                                                              >  2:1851270/1‑150 (MQ=255)
     tattaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaaaa                                                                                                                                              >  1:1687508/1‑150 (MQ=255)
      attaAGGAGCTAAAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaaaaa                                                                                                                                             <  1:1923114/150‑1 (MQ=255)
       ttaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaaaaat                                                                                                                                            <  1:1145691/150‑2 (MQ=255)
        taAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaaaaatg                                                                                                                                           >  1:2797531/1‑148 (MQ=255)
         aaGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑aaaaaaatgg                                                                                                                                          >  2:2276161/1‑147 (MQ=255)
            gAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCt                                                                                                                                      <  2:1959743/150‑1 (MQ=255)
             aGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCtt                                                                                                                                     >  1:3365851/1‑150 (MQ=255)
                 ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTgcg                                                                                                                                 >  1:789093/1‑150 (MQ=255)
                 ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTgcg                                                                                                                                 >  1:3628904/1‑150 (MQ=255)
                 ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTgcg                                                                                                                                 >  1:3573039/1‑150 (MQ=255)
                 ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTgcg                                                                                                                                 >  1:533762/1‑150 (MQ=255)
                  taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTgcgc                                                                                                                                >  1:1639074/1‑150 (MQ=255)
                      aGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCtggt                                                                                                                            <  1:4044060/150‑1 (MQ=255)
                        gTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCtggtgg                                                                                                                          <  2:4036720/150‑1 (MQ=255)
                        gTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCtggtgg                                                                                                                          <  1:274079/150‑1 (MQ=255)
                        gTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCtggtgg                                                                                                                          <  1:2788666/150‑1 (MQ=255)
                         tGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTggtggt                                                                                                                         <  1:1947531/150‑1 (MQ=255)
                          ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGGGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGgta                                                                                                                        <  1:1713462/150‑1 (MQ=255)
                          ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGgta                                                                                                                        <  2:335576/150‑1 (MQ=255)
                           gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGgtag                                                                                                                       >  2:710356/1‑150 (MQ=255)
                           gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGgtag                                                                                                                       >  2:110004/1‑150 (MQ=255)
                             gAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGgtagta                                                                                                                     >  2:1281254/1‑150 (MQ=255)
                              aaGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTaa                                                                                                                    >  2:214681/1‑150 (MQ=255)
                                  gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaa                                                                                                                >  2:790583/1‑150 (MQ=255)
                                  gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaa                                                                                                                >  2:1809827/1‑150 (MQ=255)
                                  gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaa                                                                                                                <  1:44843/150‑1 (MQ=255)
                                     tACCCGCACTTTTTCTTGACCGTGATGGCACCATTACTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAaccaacca                                                                                                             <  1:1046788/150‑1 (MQ=255)
                                         cGCTATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCt                                                                                                         <  1:3132786/150‑1 (MQ=255)
                                         cGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCt                                                                                                         <  1:591834/150‑1 (MQ=255)
                                         cGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCt                                                                                                         <  1:2073834/150‑1 (MQ=255)
                                         cGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCt                                                                                                         <  1:2073871/150‑1 (MQ=255)
                                          gCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTg                                                                                                        >  2:2406068/1‑150 (MQ=255)
                                          gCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTg                                                                                                        <  1:2114741/150‑1 (MQ=255)
                                          gCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTg                                                                                                        <  2:3191799/150‑1 (MQ=255)
                                          gCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGCGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTg                                                                                                        >  2:1693875/1‑150 (MQ=255)
                                           cAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTgg                                                                                                       <  1:1394561/150‑1 (MQ=255)
                                           cAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTgg                                                                                                       <  1:820152/150‑1 (MQ=255)
                                                 ttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGc                                                                                                 >  1:3527155/1‑150 (MQ=255)
                                                     tGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcgc                                                                                             >  1:1200788/1‑150 (MQ=255)
                                                     tGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcgc                                                                                             >  2:2248673/1‑150 (MQ=255)
                                                         cGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTa                                                                                         >  1:3986169/1‑150 (MQ=255)
                                                           tGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTaaa                                                                                       >  1:3467465/1‑150 (MQ=255)
                                                             aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGTATTTATCGTCGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAAtt                                                                                     <  1:2340674/150‑1 (MQ=255)
                                                               ggCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTa                                                                                   <  1:2353751/150‑1 (MQ=255)
                                                                gCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTAc                                                                                  >  2:3680886/1‑150 (MQ=255)
                                                                    cATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGaa                                                                              <  1:3269810/150‑1 (MQ=255)
                                                                     aTTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAg                                                                             <  2:2131610/150‑1 (MQ=255)
                                                                       tAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGca                                                                           <  2:160411/150‑1 (MQ=255)
                                                                       tAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGca                                                                           <  2:241208/150‑1 (MQ=255)
                                                                         aTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGcaca                                                                         >  1:3236582/1‑150 (MQ=255)
                                                                          tGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAg                                                                        >  2:1944373/1‑150 (MQ=255)
                                                                           gTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGt                                                                       >  1:2390439/1‑150 (MQ=255)
                                                                            tCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGGGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGtt                                                                      >  2:3298235/1‑150 (MQ=255)
                                                                                tCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGaa                                                                  >  1:978154/1‑150 (MQ=255)
                                                                                 cACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGaaa                                                                 <  2:2882436/150‑1 (MQ=255)
                                                                                  aCGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAAc                                                                >  1:2334567/1‑150 (MQ=255)
                                                                                   cGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACg                                                               <  2:839179/150‑1 (MQ=255)
                                                                                           tCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGa                                                       <  2:2887590/150‑1 (MQ=255)
                                                                                            ccATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAg                                                      <  1:2919573/150‑1 (MQ=255)
                                                                                             cATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGt                                                     <  2:634772/150‑1 (MQ=255)
                                                                                                gagaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtgga                                                  <  2:3628904/150‑1 (MQ=255)
                                                                                                 agaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggat                                                 >  1:2981532/1‑150 (MQ=255)
                                                                                                   aTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggatgg                                               <  2:3578506/150‑1 (MQ=255)
                                                                                                    tCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggatgga                                              <  2:3960927/150‑1 (MQ=255)
                                                                                                     cGACAACATAGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGAc                                             <  2:2795605/150‑1 (MQ=255)
                                                                                                      gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                            >  2:2321268/1‑150 (MQ=255)
                                                                                                      gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                            >  2:2519175/1‑150 (MQ=255)
                                                                                                      gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                            >  2:2928254/1‑150 (MQ=255)
                                                                                                      gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                            >  1:2887687/1‑150 (MQ=255)
                                                                                                        cAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTgg                                          >  1:4120807/1‑150 (MQ=255)
                                                                                                         aaCTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGAGGTAAATTTACCGAAGCACAGTTTGAAACTCTGACCGAGTGGATGGACTGGt                                         >  2:1977677/1‑150 (MQ=255)
                                                                                                          aCTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTc                                        <  2:3342285/150‑1 (MQ=255)
                                                                                                             ttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATAGCTCGCGGTAAAATTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCt                                     >  1:692294/1‑150 (MQ=255)
                                                                                                               gAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTgg                                   <  1:1433202/150‑1 (MQ=255)
                                                                                                                 aTTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCg                                 >  1:3383653/1‑150 (MQ=255)
                                                                                                                  tttATCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCgg                                >  2:924459/1‑150 (MQ=255)
                                                                                                                     aTCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGAcc                             <  1:2645320/150‑1 (MQ=255)
                                                                                                                     aTCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGAcc                             >  2:4044756/1‑150 (MQ=255)
                                                                                                                       cGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTTCGCTGGTTGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCga                           <  1:3921879/150‑1 (MQ=255)
                                                                                                                       cGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGTGTGGATGGACTGGTCGCTGGCGGACCga                           <  2:3830576/150‑1 (MQ=255)
                                                                                                                       cGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCga                           <  2:739668/150‑1 (MQ=255)
                                                                                                                         aCGGTGGTATTGACGCCATGCGCGAGCT‑‑AAAACAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATGTACCGCAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCgaga                         <  1:2491669/150‑1 (MQ=255)
                                                                                                                             tgtTATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc                     <  1:899602/150‑1 (MQ=255)
                                                                                                                                tATTGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGAt                  <  1:2303971/150‑1 (MQ=255)
                                                                                                                                  ttGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt                >  2:4096852/1‑150 (MQ=255)
                                                                                                                                  ttGACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt                <  2:2602279/150‑1 (MQ=255)
                                                                                                                                    gACGCCATGCGCGAGCTAAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt                <  1:2739921/150‑1 (MQ=255)
                                                                                                                                    gACGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTATGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTgg              >  2:1352251/1‑150 (MQ=255)
                                                                                                                                     aCGCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa             >  2:267919/1‑150 (MQ=255)
                                                                                                                                       gCCATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATg           <  2:2475519/150‑1 (MQ=255)
                                                                                                                                         cATGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGt         <  2:971653/150‑1 (MQ=255)
                                                                                                                                          aTGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTa        <  2:3467465/150‑1 (MQ=255)
                                                                                                                                           tGCGCGAGCT‑‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTGTGGCATTGCTCGCGGTAATTTTACCGAAGCACTGTTTGAAACGGTGAGCGTGTGGATGGAGTGGTCGCTGGCGGAGGGAGATGTCGTTGTGGTTGGTAt       >  2:422493/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
TGCCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCTATT  >  NC_000913/222809‑223103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑