Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,015,093 G→T 6.3% D681Y (GAT→TAT)  metE → 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,015,0930GT 6.3% 873.1 / 3.9 271D681Y (GAT→TAT) metE5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (86/157);  new base T (26/2);  total (112/159)
Fisher's exact test for biased strand distribution p-value = 2.57e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

GGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACG  >  NC_000913/4014945‑4015242
                                                                                                                                                    |                                                                                                                                                     
ggCGAAAGATGACACACAAATCCACACACACATGTGTTATTGCGAGTTCAACGACATCAAGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGa                                                                                                                                                      <  2:4117201/150‑1 (MQ=255)
   gAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTa                                                                                                                                                   >  1:3842902/1‑150 (MQ=255)
   gAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTa                                                                                                                                                   >  2:3407957/1‑150 (MQ=255)
   gAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGAAGAGTTTGATTa                                                                                                                                                   >  2:1009548/1‑150 (MQ=255)
   gAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGGGTGGAGAGTTTGATTa                                                                                                                                                   >  2:207431/1‑150 (MQ=255)
    aaaGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTAt                                                                                                                                                  <  1:819324/150‑1 (MQ=255)
    aaaGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTAt                                                                                                                                                  >  2:3182502/1‑150 (MQ=255)
     aaGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATc                                                                                                                                                 <  1:358039/150‑1 (MQ=255)
     aaGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGAATAGTTTtattata                                                                                                                                                 >  1:1078644/1‑149 (MQ=255)
     aaGATGACACACAAATCCACACTCACATGTGTTAGTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATc                                                                                                                                                 <  1:3795936/150‑1 (MQ=255)
      aGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATcc                                                                                                                                                <  1:2392342/150‑1 (MQ=255)
       gATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCa                                                                                                                                               >  1:2018400/1‑150 (MQ=255)
       gATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCa                                                                                                                                               <  2:829995/150‑1 (MQ=255)
       gATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGAAGAGTTTGATTATCCa                                                                                                                                               >  2:2918500/1‑150 (MQ=255)
        aTGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGGAGAGTTTGATTATCCaa                                                                                                                                              >  2:1472733/1‑150 (MQ=255)
            cacacaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGTATAGTTTTTTTATACAAATGa                                                                                                                                          >  2:60021/1‑150 (MQ=255)
             acacaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGGAGAGTTTGATTATCCCAATGaa                                                                                                                                         >  1:3296144/1‑150 (MQ=255)
             acacaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGaa                                                                                                                                         >  2:241292/1‑150 (MQ=255)
               acaACTCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAAt                                                                                                                                       <  2:3471510/150‑1 (MQ=255)
               acaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGTAGAGTTTGTTTATCCAAATGaaaa                                                                                                                                       >  1:1927472/1‑149 (MQ=255)
               acaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGTAGAGTTTGATTTTGCAAATGAAAt                                                                                                                                       >  1:2755516/1‑150 (MQ=255)
               acaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAAt                                                                                                                                       <  1:1861130/150‑1 (MQ=255)
               acaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAAt                                                                                                                                       <  2:2543955/150‑1 (MQ=255)
               acaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAAt                                                                                                                                       <  1:5609/150‑1 (MQ=255)
                 aaaTCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCg                                                                                                                                     <  1:3270202/150‑1 (MQ=255)
                 aaaTCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCg                                                                                                                                     <  2:2304509/150‑1 (MQ=255)
                 aaaTCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCg                                                                                                                                     <  1:2119471/150‑1 (MQ=255)
                 aaaTCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCg                                                                                                                                     <  2:875561/150‑1 (MQ=255)
                       acacTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTg                                                                                                                               >  2:2160269/1‑150 (MQ=255)
                         acTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGc                                                                                                                             >  2:3774313/1‑150 (MQ=255)
                           tCACTTGTGTTATTGAGAGTTCAACGACATCATGGTTTCGATTGCGGCGCTGGACGCAGACGTCATCACAATCGAAACCTCGCGTTCCGACATGGAGTTGATGGTGTCGTTTGAAGAGTTTTATTATCCAAATGAAATCGGTCCTGGCGt                                                                                                                           <  1:1107142/150‑1 (MQ=255)
                           tCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTTATTATCCAAATGAAATCGGTCCTGGCGt                                                                                                                           >  2:2756668/1‑150 (MQ=255)
                           tCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGt                                                                                                                           <  2:2492190/150‑1 (MQ=255)
                             acaTGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGAAGAGTTTGATTATACAAATTAAATAGGTACTGTCGTCt                                                                                                                         >  2:2572723/1‑150 (MQ=255)
                                 gtgtTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACATCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTTATTATCCAAATGAAATCGGTCCTGGCGTCTATGa                                                                                                                     >  2:316498/1‑150 (MQ=255)
                                     tagtGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACAtt                                                                                                                 <  1:1775849/147‑1 (MQ=255)
                                     tATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACAtt                                                                                                                 <  2:1685234/150‑1 (MQ=255)
                                     tATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATACAAATGAAATCGGTCCTGGCGTCTATGACAtt                                                                                                                 >  1:292969/1‑150 (MQ=255)
                                      aTTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGGAGAGTTTGATTATACAAAATAAATAGGTCCAGGCGGCTATGACAATc                                                                                                                >  2:1066399/1‑150 (MQ=255)
                                       ttGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCa                                                                                                               <  1:875803/150‑1 (MQ=255)
                                          cGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAAAAGGTCCTGGCGTCTATGACATTCACTc                                                                                                            >  1:3703708/1‑150 (MQ=255)
                                           gAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTTATTATCCAAATGAAAACGGTCCTGGCGTCTATGACAATCACACg                                                                                                           >  2:1202084/1‑150 (MQ=255)
                                           gAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCg                                                                                                           <  1:3153982/150‑1 (MQ=255)
                                              ttCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGGAGAGTTTTATTATCCAAATGAAATCGGTCCAGGCGTCGATGACATTCACACGCCa                                                                                                        >  1:3027090/1‑150 (MQ=255)
                                               tCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTTAAGAGTTTGATTATCCAAAAGTAAACGGTACATGCGTCTATGAAAATCACAAGCCaa                                                                                                       >  1:693196/1‑150 (MQ=255)
                                                cAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCaaa                                                                                                      <  1:2703739/150‑1 (MQ=255)
                                                 aaCGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAATAGTTTGATTAACCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAAc                                                                                                     >  2:2707840/1‑150 (MQ=255)
                                                  aCGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTTATTATCCAAATGAAATAGGTCCTGGCGTCTATGACAATAACACGCCaaaag                                                                                                    >  2:1289333/1‑148 (MQ=255)
                                                    gACATCATGGATTCGATTGAGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTa                                                                                                  <  2:95726/150‑1 (MQ=255)
                                                     aCATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTAc                                                                                                 <  1:1516558/150‑1 (MQ=255)
                                                     aCATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTAc                                                                                                 <  1:2049308/150‑1 (MQ=255)
                                                           tGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAAAGAAATCGGTCCTGGCGTCTATGACATTAACTCGCCAAACGTACCGAGCg                                                                                           >  2:119163/1‑150 (MQ=255)
                                                             gATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTTTTTATCCAAATGAAAAAGGTCCAGGCGTCGATGACAATCACTCGCCCAACGAACCGAGCGTg                                                                                         >  1:731883/1‑150 (MQ=255)
                                                             gATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGTAGATTTTTATTATACAAAATAAAAAGGTCCAGGCGTGTAAGTCATTAACACGCCCAACCTTACGAGCGTg                                                                                         >  2:699240/1‑150 (MQ=255)
                                                              aTTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATACAAAAGAAAACGGTCCGGGCGGCTATGACAATCACTCGCCACACGTACCGCGCGTgg                                                                                        >  1:3511307/1‑150 (MQ=255)
                                                                tCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGaa                                                                                      <  1:4077282/150‑1 (MQ=255)
                                                                    ttGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGa                                                                                  <  1:843056/150‑1 (MQ=255)
                                                                    ttGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGa                                                                                  <  1:3006306/150‑1 (MQ=255)
                                                                         gcgcTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGaa                                                                             <  1:1377581/150‑1 (MQ=255)
                                                                          cgcTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGTAGAGTTTGATTATCCAAATTAAATCGGTCCTGGCGTCGATGTCAATAACTAGCCAAACGTACCCAGCGTGGGCTGGCTTGAAg                                                                            >  1:499337/1‑150 (MQ=255)
                                                                          cgcTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGAAGGGTTTGATTATCCAAATTAAAACGGTCCGGGCGTCTATGACATTCACTCGCCAAACGTAACGAGCGTGGAAAGGATTGAAg                                                                            >  1:1694554/1‑150 (MQ=255)
                                                                              ggACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCtt                                                                        <  2:2868135/150‑1 (MQ=255)
                                                                                aCGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGc                                                                      <  2:1110953/150‑1 (MQ=255)
                                                                                aCGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGc                                                                      <  1:464345/150‑1 (MQ=255)
                                                                                 cGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCt                                                                     <  2:3988014/150‑1 (MQ=255)
                                                                                   cAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTga                                                                   <  1:3182502/150‑1 (MQ=255)
                                                                                        gTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGTAGAGTTTGTTTATCCAAATGAAATAGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGGATTGGTTGAAGCCGTGCTGAAGaaa                                                              >  1:3419215/1‑150 (MQ=255)
                                                                                          catcaCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTTATTATACAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGc                                                            >  2:1247519/1‑150 (MQ=255)
                                                                                          catcaCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGc                                                            <  1:252856/150‑1 (MQ=255)
                                                                                           atcaCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCg                                                           <  1:2234706/150‑1 (MQ=255)
                                                                                              aCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATg                                                                                    >  2:2018484/1‑122 (MQ=255)
                                                                                                cATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCaaa                                                      <  1:2812079/150‑1 (MQ=255)
                                                                                                cATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCaaa                                                      <  2:3370291/150‑1 (MQ=255)
                                                                                                 aTCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCaaaa                                                     <  2:3356365/150‑1 (MQ=255)
                                                                                                  tCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAAc                                                    <  1:1276789/150‑1 (MQ=255)
                                                                                                  tCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTGTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGGCGATGACATTCACACGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCCAAAc                                                    >  2:159432/1‑150 (MQ=255)
                                                                                                    gAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGc                                                  <  1:2294919/150‑1 (MQ=255)
                                                                                                       aCCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGTAGAGTTTGATTAAACAAAAGAAATAGGTCCAGGCGTCTATGACATTCACACCCCAAACGTACCGAGCGTGGAATTGATTGTAGCCATGCTGAAGAAAGCGGCAAAAAGCaat                                               >  2:2455877/1‑148 (MQ=255)
                                                                                                           cgcgTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCgg                                           <  1:1708576/150‑1 (MQ=255)
                                                                                                           cgcgTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCgg                                           <  2:1501147/150‑1 (MQ=255)
                                                                                                              gTTCCGACATGGAGTTGCTGGAGTCGTGTGAAGAGTTTGATTATACCAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAAAGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCag                                        >  2:3731664/1‑150 (MQ=255)
                                                                                                                 ccGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAgc                                     <  1:2866848/150‑1 (MQ=255)
                                                                                                                     cATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGAAATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAATCCATTCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCt                                 >  2:312022/1‑150 (MQ=255)
                                                                                                                         gAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTgg                             <  1:82875/150‑1 (MQ=255)
                                                                                                                            ttGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTc                          <  1:1920000/150‑1 (MQ=255)
                                                                                                                                    gTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGAt                                                                                 >  2:815796/1‑87 (MQ=255)
                                                                                                                                    gTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGa                  <  1:55346/150‑1 (MQ=255)
                                                                                                                                        tttGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACtgt              >  2:3086645/1‑150 (MQ=255)
                                                                                                                                        tttGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACtgt              <  1:1289333/150‑1 (MQ=255)
                                                                                                                                        tttGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACtgt              <  2:2638022/150‑1 (MQ=255)
                                                                                                                                           gAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGc           <  1:4038028/150‑1 (MQ=255)
                                                                                                                                           gAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGc           <  2:553887/150‑1 (MQ=255)
                                                                                                                                           gAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGc           <  2:1936352/150‑1 (MQ=255)
                                                                                                                                           gAAGAGTTTGATGATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGc           <  2:3305071/150‑1 (MQ=255)
                                                                                                                                           gAAGAGTTTGATGATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGc           <  2:922695/150‑1 (MQ=255)
                                                                                                                                           gAAGAGTTTGATCATCCAAATGAAATCGGTCCTGGCGTCTATGACAGTCACTCGCCACACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGc           <  2:2253309/150‑1 (MQ=255)
                                                                                                                                            aaGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGcc          >  1:1421943/1‑150 (MQ=255)
                                                                                                                                                 tttGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGaaa     >  2:3220171/1‑150 (MQ=255)
                                                                                                                                                 tttGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGaaa     >  1:263739/1‑150 (MQ=255)
                                                                                                                                                    gATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAAcg  >  2:2972502/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACG  >  NC_000913/4014945‑4015242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑