BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	453,599	T→G	5.7%	N190T (AAC→ACC)	yajG ←	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,599	0	T	G	5.7%	656.2 / 3.6	241	N190T (AAC→ACC)	yajG	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (101/120); new base G (2/16); total (103/138)
Fisher's exact test for biased strand distribution p-value = 5.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

GGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCAT  >  NC_000913/453450‑453747

ggATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGt                                                                                                                                                      >  2:3580905/1‑150 (MQ=255)

 gATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAAGATGGCTGAACGCGGCTGTTGAAAAATACGTAAATAGTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGtt                                                                                                                                                     <  2:1906559/150‑1 (MQ=255)

     tCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGc                                                                                                                                                 >  1:918724/1‑150 (MQ=255)

       aGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCtt                                                                                                                                               >  1:3730142/1‑150 (MQ=255)

       aGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCtt                                                                                                                                               >  2:1973240/1‑150 (MQ=255)

       aGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGGTCTGCtt                                                                                                                                               <  1:1555630/150‑1 (MQ=255)

         ggCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTtga                                                                                                                                             <  2:3830669/150‑1 (MQ=255)

         ggCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTtga                                                                                                                                             >  1:3354740/1‑150 (MQ=255)

            gAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTtgatga                                                                                                                                          >  2:2637650/1‑150 (MQ=255)

              gCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAAt                                                                                                                                        <  2:2135977/150‑1 (MQ=255)

              gCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAAt                                                                                                                                        >  1:612588/1‑150 (MQ=255)

               cGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAAtt                                                                                                                                       >  2:1281013/1‑150 (MQ=255)

                    gCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGGCATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTgg                                                                                                                                  <  1:1822837/150‑1 (MQ=255)

                    gCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTgg                                                                                                                                  <  1:3284156/150‑1 (MQ=255)

                     cccGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGa                                                                                                                                 <  1:510320/150‑1 (MQ=255)

                     cccGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGa                                                                                                                                 >  1:245237/1‑150 (MQ=255)

                       cGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATg                                                                                                                               >  2:574994/1‑150 (MQ=255)

                          aaGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTg                                                                                                                            <  2:31557/150‑1 (MQ=255)

                          aaGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTg                                                                                                                            <  1:2996566/150‑1 (MQ=255)

                            gCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGgt                                                                                                                          <  1:491958/150‑1 (MQ=255)

                             cAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGgtg                                                                                                                         >  1:1251131/1‑150 (MQ=255)

                                 aCCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCt                                                                                                                     <  2:1612392/150‑1 (MQ=255)

                                 aCCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCt                                                                                                                     <  1:1052664/150‑1 (MQ=255)

                                 aCCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCt                                                                                                                     <  2:3658001/150‑1 (MQ=255)

                                   ccAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTga                                                                                                                   >  2:1159257/1‑150 (MQ=255)

                                     aGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTgaga                                                                                                                 <  1:499378/150‑1 (MQ=255)

                                      ggATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGAc                                                                                                                <  2:2903944/150‑1 (MQ=255)

                                          cAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACatat                                                                                                            >  1:3007980/1‑150 (MQ=255)

                                          cAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACatat                                                                                                            <  2:3196012/150‑1 (MQ=255)

                                           aGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATc                                                                                                           >  1:3922254/1‑150 (MQ=255)

                                            gCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATct                                                                                                          >  2:429682/1‑149 (MQ=255)

                                             cAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAg                                                                                                         <  1:4067265/150‑1 (MQ=255)

                                             cAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAg                                                                                                         >  1:1977130/1‑150 (MQ=255)

                                             cAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAg                                                                                                         <  1:2657488/150‑1 (MQ=255)

                                              aaTATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGc                                                                                                        <  1:73749/150‑1 (MQ=255)

                                                tatGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGa                                                                                                      <  1:604184/150‑1 (MQ=255)

                                                tatGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGa                                                                                                      <  1:3882556/150‑1 (MQ=255)

                                                gagggCTGAACGCGGCCGACGAAAAAGACGTACAGCGGGACTGGACCTGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAAGTACCGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATCTCAGCGa                                                                                                      <  2:3061084/147‑1 (MQ=255)

                                                 atGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGAt                                                                                                     <  2:3987428/150‑1 (MQ=255)

                                                 atGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGAt                                                                                                     <  1:1674514/150‑1 (MQ=255)

                                                  tGGCTGACCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACCTGGGCGGCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATg                                                                                                    <  1:2775189/150‑1 (MQ=255)

                                                    gCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGt                                                                                                  <  2:2985357/150‑1 (MQ=255)

                                                    gCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGt                                                                                                  <  2:918724/150‑1 (MQ=255)

                                                     cTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCCGAGACATATCAGCGATGGTa                                                                                                 >  1:3569032/1‑150 (MQ=255)

                                                      tGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTAt                                                                                                <  1:597/150‑1 (MQ=255)

                                                        aaCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCg                                                                                              >  2:406839/1‑150 (MQ=255)

                                                        aaCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCg                                                                                              >  1:980455/1‑150 (MQ=255)

                                                        aaCGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCg                                                                                              >  1:1998918/1‑150 (MQ=255)

                                                           gcgGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCGTGATGAATTCGTGGATGCTGGTGTCCTGAGACATCTCAGCGATGGTATCGGTc                                                                                           >  2:441544/1‑150 (MQ=255)

                                                            cgGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCa                                                                                          <  2:3834423/150‑1 (MQ=255)

                                                            cgGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCa                                                                                          <  1:3606367/150‑1 (MQ=255)

                                                             ggCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAg                                                                                         >  2:1814357/1‑150 (MQ=255)

                                                               cTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGca                                                                                       <  2:2776313/150‑1 (MQ=255)

                                                                    gAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTGCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGTACACTg                                                                                  <  2:69522/150‑1 (MQ=255)

                                                                             gTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCa                                                                         >  1:3487290/1‑150 (MQ=255)

                                                                             gTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCa                                                                         >  2:15040/1‑150 (MQ=255)

                                                                             gTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATAAGCGATGGTATCGGTCAGCACACTGTTAACCGCa                                                                         >  1:1417778/1‑150 (MQ=255)

                                                                              tAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCAt                                                                        <  2:956900/150‑1 (MQ=255)

                                                                               aaaTATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGAGGGTATCGGTCAGCACACTGTTAACCGCATc                                                                       >  2:1307902/1‑150 (MQ=255)

                                                                                    ttGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGa                                                                  >  2:1054553/1‑150 (MQ=255)

                                                                                           gACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATAttttt                                                           <  1:2870921/150‑1 (MQ=255)

                                                                                           gACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATAttttt                                                           <  2:188735/150‑1 (MQ=255)

                                                                                           gACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCGGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATAttttt                                                           >  2:1767853/1‑150 (MQ=255)

                                                                                            aCATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTttg                                                          <  2:3790463/150‑1 (MQ=255)

                                                                                             cctGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGTGGGCATATATTAAAGCGAGCTCTGCTTGTTGTATTCGTGGATGCTGGTGTCCTTAGACCTTTCAGCTATGGTTTCTGTCAGCACAATGTTAACCGCATCGGCGATATTTttgt                                                         <  2:621781/148‑1 (MQ=255)

                                                                                              aTGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTttgtt                                                        <  2:2521563/150‑1 (MQ=255)

                                                                                               tGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTttgttg                                                       >  2:789818/1‑150 (MQ=255)

                                                                                                gggCGTCTACAGGCCCGGCTTGCACCGGGCCCGAGGGCAGAAATTAACGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTgg                                                      >  1:1838138/1‑150 (MQ=255)

                                                                                                 ggCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGa                                                     >  2:102918/1‑150 (MQ=255)

                                                                                                 ggCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGa                                                     <  2:1016504/150‑1 (MQ=255)

                                                                                                 ggCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGa                                                     <  2:3272763/150‑1 (MQ=255)

                                                                                                  gCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTCCCGCGCGTGCTGCTGGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAg                                                    <  2:47465/150‑1 (MQ=255)

                                                                                                  gCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACAAATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAg                                                    >  1:1744217/1‑150 (MQ=255)

                                                                                                    gTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGc                                                  >  1:3026737/1‑150 (MQ=255)

                                                                                                          aGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTCGAGGCCTGGaa                                            <  1:2246609/150‑1 (MQ=255)

                                                                                                            gCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACg                                          >  2:2016505/1‑150 (MQ=255)

                                                                                                               cGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCAc                                       <  2:3324735/150‑1 (MQ=255)

                                                                                                                     gCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCaa                                 >  1:337115/1‑150 (MQ=255)

                                                                                                                     gCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCaa                                 <  2:3374359/150‑1 (MQ=255)

                                                                                                                      cACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAAc                                >  1:2982374/1‑150 (MQ=255)

                                                                                                                       aCCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACg                               >  1:2160906/1‑150 (MQ=255)

                                                                                                                       aCCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACg                               >  2:479906/1‑150 (MQ=255)

                                                                                                                       aCCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACg                               >  1:3191483/1‑150 (MQ=255)

                                                                                                                        ccGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGt                              >  1:326517/1‑150 (MQ=255)

                                                                                                                         cGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGtt                             <  2:2204228/150‑1 (MQ=255)

                                                                                                                          gggCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTg                            <  1:3974101/150‑1 (MQ=255)

                                                                                                                              cAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGc                        >  1:176832/1‑150 (MQ=255)

                                                                                                                                 agGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTgg                     <  1:524665/150‑1 (MQ=255)

                                                                                                                                        aaaTTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATa              >  2:89433/1‑150 (MQ=255)

                                                                                                                                           ttAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGtt           <  2:1955411/150‑1 (MQ=255)

                                                                                                                                              aCGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGttttt        <  1:468596/150‑1 (MQ=255)

                                                                                                                                              aCGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGttttt        <  2:1786606/150‑1 (MQ=255)

                                                                                                                                              aCGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGttttt        <  1:305499/150‑1 (MQ=255)

                                                                                                                                              aCGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGttttt        <  2:2850274/150‑1 (MQ=255)

                                                                                                                                              aCGCGCGGTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGttttt        <  1:132650/150‑1 (MQ=255)

                                                                                                                                                gcgcgTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTgg      >  1:1906725/1‑150 (MQ=255)

                                                                                                                                                gcgcgTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTgg      >  1:2837985/1‑150 (MQ=255)

                                                                                                                                                gcgcgTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGATGGCACGATAGTTTTTgg      >  1:3327014/1‑150 (MQ=255)

                                                                                                                                                 cgcgTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGt     <  1:1897934/150‑1 (MQ=255)

                                                                                                                                                    gTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCAt  <  2:3487290/150‑1 (MQ=255)

GGATGTCAGGGCGAGCGGTAGCCCGGAAGCAAAACCCAGGATCAGCAATATGGCTGAACGCGGCTGTTGAAAAATACGTAAATATTGACTGGACATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCAT  >  NC_000913/453450‑453747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`