| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,581 | A→C | 17.1% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,581 | 0 | A | C | 17.1% | 150.4 / 14.7 | 96 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (27/51); new base C (4/14); total (31/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164433‑164730 | cGTGAGGTGCAAAAAGAGATGAACGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt < 2:912589/150‑1 (MQ=255)cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:1283904/1‑150 (MQ=255)cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt < 2:3723365/150‑1 (MQ=255)cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:3412140/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc > 1:252046/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc > 1:2439683/1‑150 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGt > 1:2071665/1‑150 (MQ=255) ctagaTGAAGGGGGGTTAGCCCAAACCTGTCTGGCCGGACGCCCCGGCAAATACTGCCCCGAGGGGAGGGAGTAAAATTTTTTGGTATGTTTGGTTTTCGCCCACGCGCTGTGGGTTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtc < 2:2097147/148‑1 (MQ=255) agatATGACAGGGCTTTATCCCAAACAGGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGATTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtc < 2:2250362/150‑1 (MQ=38) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTtcaccc > 2:3097106/1‑150 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGtcaccc > 2:2997873/1‑150 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTggaccc > 1:3667663/1‑150 (MQ=255) gatcgggCGTTATCCCAAACTTGTGTGGCGGGACGACCCGGCAAATACTGCACCTACGCGAGTGACTAAAATTTATTCTTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTTAGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcaccctc < 2:915362/146‑1 (MQ=25) aatgggCTTTATCCCAAACATGGCTGGCGGGACGACCCGGCAAATACTGCACCGACGCGAGGGACGAAAAATTTTTCGTATGGTTGTTTTTCTCCCTCCCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcaccctca < 2:461854/147‑1 (MQ=255) aaGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGAAATTACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac < 2:2085075/150‑1 (MQ=38) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCACATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGGCACCCCCCCCCCAAc > 2:2172603/1‑150 (MQ=38) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCACCCaaaac > 1:1628778/1‑148 (MQ=255) ttACCCCAACCTGGTCGGGCGGGACGCCCCGGAAAATACTGCCCGGACGCGACGGACTAAAATGTTTTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:2293466/150‑1 (MQ=25) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGACCCACCCCCAAAcccaca > 1:1387512/1‑147 (MQ=38) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCAAAaccaca > 1:2362583/1‑147 (MQ=38) ccAAACTTGTGGGGCGGGCCGACCCGCAAAATACTGCACGGACGGTACGCACTAAAAATTATTCGTAGGGTTGTTTTTCTCCCTCCCCGGGTGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:377983/150‑1 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGACCCCCACCCAAAaccccac > 1:1772929/1‑146 (MQ=38) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTGCCCCCCCCCCCACCCCtctc > 2:3283247/1‑150 (MQ=37) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGACCCCCACCCCAAcccacaa > 2:1470220/1‑146 (MQ=38) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCTCCCGCACCCAAAaccacaa > 1:2566152/1‑146 (MQ=38) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCCCCCACAcccccgc > 1:2127143/1‑146 (MQ=37) cAACCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCCCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:979399/150‑1 (MQ=255) cAAACTGGTCTGGCCGGACGACCCGGAAAATACTGCCCCGACGCGACGGACTAAAATTTATTCGTACGGTTGTTTTTCTCCCTCCCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:400521/150‑1 (MQ=37) caccATGCCGGGCCGGCCGCCCGGCCAACTACGGCACCTACGCGAGGGCCGAAAATTTATTCTTAGGGTGGGTTTTCGCCCTCTCCGTGGGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 2:387806/147‑1 (MQ=255) aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 2:3069829/150‑1 (MQ=255) aaaCAGGTGGGGCCGGCCGACGCGGAAATTCCGGCACCTAGGCTTGGCACTAAAATGTATTCGTTTGTTTGTTTTTCGCCCTCCCCTTGTGGGTGCGGGCGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 2:387813/150‑1 (MQ=255) aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCAACCTAAACCACACCCACaa > 2:2401631/1‑150 (MQ=37) gtgtGGCGGGACGCCCGGGCAAATCCGGCCCCGACGGGAGGGACGAAAATGTTTTCTTAGGGTTGGTTTCCTCCCCCGCCCGGTGGGCGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 2:2854694/147‑1 (MQ=255) gTCTGGCCGGACGACCCGGCAAATAGTGCACCGACGCGACGGACGAAAAAGTTTTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 2:1916043/150‑1 (MQ=255) gTCGGGCCGGCCGACCCGGAAAATCCTGCCCCGACGCGCCGGACTAAAAATTATGCGTTTGGTTGGTTTTCTCCCTCGCCCTGGGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 1:3420204/150‑1 (MQ=255) gggCCGGACGACCCGCAAAATCCTGCCCCGACGCGACGGACCAAAAATTATTCTTATGGTTGTTTTTCCCCCTCGCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:1972141/149‑1 (MQ=255) ggCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCAAACCCACACCCACCAGGggga > 1:3088393/1‑150 (MQ=37) ggCCGGACGACCCGGAAAATACGCCCCCGACGCGACGGACGAAAAAGTTTTGGTTTGGTTGGTTTTCACCCTCCCCCTGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 2:3206587/150‑1 (MQ=255) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGCGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGTCCCGCACCCGAAACCACTCCCGCCAggggggg > 1:1913013/1‑146 (MQ=37) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCTAACCCTCACCCACCAGGGagag > 2:3081870/1‑150 (MQ=255) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCCCCCGAAACCTCTCCCACACgggggcg > 2:3081373/1‑146 (MQ=37) cggtacgccCCGCAAAATACTGCACCGACGCGAGGTACTAAAATTTTTTCGTATGGTTGGTTTTCTCCCTCTCCTTGTGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 1:2755702/146‑1 (MQ=255) cGGCCGCCCGGGCAACTCCGGCCCCGCCGCGCCGGACGACACCGTTGGCGTAGGGTGGGTTTTCGCCCGCCCCGGGGGGGGGCGGGCGGGGGGGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:2957533/150‑1 (MQ=255) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCACACCCAAACCCTCTCCCGCCAggggggggggg > 1:1606071/1‑149 (MQ=37) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACCCCCCAACCCACAACCACAAGGGAggggggg > 1:2015819/1‑149 (MQ=25) cgccccGGCATATACGGCACCGACGCGCCGGACGAAAACGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc < 2:3746142/147‑1 (MQ=38) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACCCCCCAACCCCCGGCCGAAAGGGCGCGGGGACCgcgc > 2:1587480/1‑147 (MQ=25) gCAAATCCGGCCCCGCCGCGCCGGACGAAACGGTATGGGTCTGGTGGGTTTTCGCCCCCGCCGTGGGGGCGCGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:772522/150‑1 (MQ=255) gCAAATACTGCCCCGCCGCGCCGGACGAAAACGTTTTCGTCCGGTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:3544166/150‑1 (MQ=37) gCAAAGACGGCCCCGCCGCGACGGACGAAAAAGTATTCGTAGGGTTGGTTTCCCCCCTCGCCCTGTGGGAGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:2945254/150‑1 (MQ=255) aaaTCCGGCACCTCCGCGACGGACTAAAATTTTTGCTTATGGTTGTTTTTCCCCCCCGCCGTGTGGGTGCGGGCGGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 1:576923/150‑1 (MQ=255) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCACACCCCCAACCCCACCCCCCAGGGGGGGGGGGCCGCTCgcgcc > 1:2162665/1‑149 (MQ=21) ccccgccgCGCCGTACGAAAAAGTTTTCTTACGGTTGTTTTACCCCCTCTCCCGGGGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:1926968/148‑1 (MQ=255) cACCGACGCGCCGGACTAAAATTTATTGTTAAGGTTGGTTTTCTCCCTCTCCGTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:3570652/150‑1 (MQ=255) cACCGACGCGAGGTACTAAAATTTTGTCGTATGGTTGGTTTTCTCCCTCCCCGTGGGGGTTTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:2580415/150‑1 (MQ=255) cACCGACGCGACGTACTAAAAATTTTGCTTATGTTGGGTTTTCTCCCTCTCCCGGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:2466674/150‑1 (MQ=37) aCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCGAACCCACTACCACCAGGGAGAGGGGCCCCAGCGCGCACaaaaattt > 1:926401/1‑145 (MQ=25) ccGACGCGCCGGACTAAAAAGTATTCTTATGGTTGGTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:252046/150‑1 (MQ=38) ccGACGCGCCGGACGAAAAATTATTCTTAAGTTTTGTTTTCTCCCTCTCCCTTTGGGCTTGGGCCGGTGTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACACTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:182005/150‑1 (MQ=255) ccGACGCGACGTCCGAAAAATTATTCTTACGGTTGTTTTTCTCCCTCTCCGTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3858420/150‑1 (MQ=38) ccGACGCGACGGACTAAAAATTATTCGTATGGTTGGTTTTCTCCCCCTCCGTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:2966773/150‑1 (MQ=38) ccGACGCGACGGACGAAAAAGTTTTCGTATGGTTGGTTTTCGCCCTCGCCGTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2439683/150‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2071665/150‑1 (MQ=255) acgcgacgGACGAAAATGTATTCGTACGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag < 2:3726567/150‑1 (MQ=255) gacggactAAAATTTTTTCGTTTGGTTGGTTTTCCCCCTCCCCCTGTGGTAGAGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:267055/150‑1 (MQ=255) tcggacgAAATAGTTTTCGTTTGGTGGGTTTTCTCCCTCTCGGGGGGGGTGTGGGGGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 1:2622196/149‑1 (MQ=255) cgAAAATGTATTCGTAAGGTTGTTTTACTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt < 1:2342949/150‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCACACCCAAAACAAAAACCACAAGGGAGATGGGGCCCCTCGAGGCCAAAAATTGAGAGatataataaaa > 1:1723538/1‑143 (MQ=16) aGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACACAAACCCACACCCGAAAGGGAGGGGGGACCCATAGAGGACAACTTTTGTGAGATATCTTATTATGTCt > 2:2655425/1‑150 (MQ=25) tCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 1:3412140/150‑1 (MQ=255) gtcgggttgggtTTCGCCCTCTCCGTGGGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:3593298/146‑1 (MQ=255) aGGTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:2757479/150‑1 (MQ=255) tttgtttTTCTCCCTCCCCCTGTGGGTGCGGGCCGGGTTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGTGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 1:2112707/149‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:1703734/150‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACAACCAAAACCACGACCACCAGGGGGAGGGGACCGCTCGAGCCCCCAGTGTGAGGGATATCTTAGTGTGTGTTGTGACAGAAGTAc > 2:1275698/1‑150 (MQ=12) ggttTTCTCCCTCTCCGGGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 2:3667663/150‑1 (MQ=255) ggttTTCCCCCACTCCCTGTGGGCGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTGTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:4018247/150‑1 (MQ=255) tggtagcccgctcgctGTGTGTTAGGGCGGGTGTGAGGCCATCAGCGCGATAGTTCACCCTCACCCTAACCCTCTCCCTCATGGGAGAGGGGAACGATAGAGCACAAATTTTTTGAGATATCTTCTTCTGTCTTGTAACATAATAACAGa < 1:2176256/144‑1 (MQ=255) tccgatctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAt < 1:70675/145‑1 (MQ=255) ccccccccctGTGGGTGTGGGCCGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:488405/144‑1 (MQ=255) cctctccctGGGGGAGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:2150835/150‑1 (MQ=255) ctctccctGTGGGTGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 1:2997873/150‑1 (MQ=255) gggggACAGGCCCGGTTTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcg < 2:494591/148‑1 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:4115496/150‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 2:2362583/150‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACTCCCACAAGGGCGAGGGGCCCGATCGAGCCCAAAATTTGAGAGATAAAATATTATGTCTTGTTAAACAAGAAAAGAAAAAAGGGCTTTTGCGccgg > 2:2080890/1‑148 (MQ=255) ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 2:3088393/150‑1 (MQ=255) gggggTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 2:3215342/149‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2547289/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:4120618/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:355881/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:355863/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:3257588/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 2:3553659/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:511831/150‑1 (MQ=255) gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg < 2:1723538/150‑1 (MQ=255) aTCCGCGCGCACGTGCAACCACACCCCAAACCAATCCCTCAAGGGAGAGGGGACCCAAACAGCACAAATTTTTAGAGATATAATCATAATTATTTTAAAAGAAGAAAACAAAAAACGGCTTTTTCGCCCGAATAATTAGGAGAAAAAGCa > 2:3754665/1‑150 (MQ=255) | CGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164433‑164730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |