Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,292,473 C→G 5.5% intergenic (+108/+36) galU → / ← hns glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,4730CG 5.5% 654.6 / 25.0 181intergenic (+108/+36)galU/hnsglucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
Reads supporting (aligned to +/- strand):  ref base C (90/81);  new base G (7/3);  total (97/84)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 184 total aligned reads displayed.

AAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTC  >  NC_000913/1292327‑1292619
                                                                                                                                                  |                                                                                                                                                  
aaaGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTgg                                                                                                                                                 >  1:3281251/1‑150 (MQ=255)
aaaGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTgg                                                                                                                                                 >  2:3624790/1‑150 (MQ=255)
 aaGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGTCAATAAAAAATCCCGCCGCTGGc                                                                                                                                                <  2:3759465/150‑1 (MQ=255)
   gCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCgg                                                                                                                                              <  2:1797133/150‑1 (MQ=255)
     cTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGa                                                                                                                                            <  2:2870591/150‑1 (MQ=255)
      tGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAt                                                                                                                                           >  1:3438483/1‑150 (MQ=255)
      tGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAt                                                                                                                                           >  2:1061131/1‑150 (MQ=255)
         cTTGAAGAAGAGATGGGCATTACGCAGTACCATCCGTATCGGGGGTATCCACGACACGGCGTTGAGCAATCGACGCCGTTTGTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCGGGCGGGAtttt                                                                                                                                        >  1:3469171/1‑150 (MQ=255)
           tGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtttttt                                                                                                                                      <  2:3064402/150‑3 (MQ=255)
                  gagaTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTg                                                                                                                               >  2:132455/1‑150 (MQ=255)
                     aTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAACCGGCGGTGAGCAAGCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTGTAAGCAAGTGCaa                                                                                                                            >  2:586467/1‑150 (MQ=255)
                       gggCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATc                                                                                                                          >  1:1326895/1‑150 (MQ=255)
                       gggCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATc                                                                                                                          >  2:142717/1‑150 (MQ=255)
                          cATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTAc                                                                                                                       <  2:1662549/150‑1 (MQ=255)
                             tAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaaa                                                                                                                    >  2:817453/1‑150 (MQ=255)
                              aagaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaaaa                                                                                                                   <  1:957287/150‑1 (MQ=255)
                              aagaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaaaa                                                                                                                   <  1:3022280/150‑1 (MQ=255)
                                gaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGa                                                                                                                 >  1:1347422/1‑150 (MQ=255)
                                    tAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGattat                                                                                                             <  1:173328/150‑1 (MQ=255)
                                     aaCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGattatt                                                                                                            <  2:1243819/150‑1 (MQ=255)
                                      aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTTTTAAATTTTCTTAAAACGGACAATAAAAAATACCGCCGCTGGCGGGGTTTTAAGCAAGTTCAATATACAAAAGATTATTg                                                                                                           >  1:3880782/1‑150 (MQ=255)
                                      aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                           >  2:2900221/1‑150 (MQ=255)
                                      aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                           >  2:3104281/1‑150 (MQ=255)
                                         tCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCtt                                                                                                        <  1:2331549/150‑1 (MQ=255)
                                          ccGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTg                                                                                                       <  2:2738544/150‑1 (MQ=255)
                                          ccGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTg                                                                                                       >  2:523054/1‑150 (MQ=255)
                                           cGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGa                                                                                                      <  1:1583137/150‑1 (MQ=255)
                                            gTATCGGTGTTATCCACGAAACGGCGTTGAGCACTCGACGCCGTTTTTTTATAGCTTAGTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGAt                                                                                                     >  1:2935896/1‑150 (MQ=255)
                                            gTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGAt                                                                                                     <  2:292522/150‑1 (MQ=255)
                                                cGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                                                                                                 <  2:121577/150‑1 (MQ=255)
                                                cGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                                                                                                 <  1:174118/150‑1 (MQ=255)
                                                cGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                                                                                                 <  1:560973/150‑1 (MQ=255)
                                                cGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                                                                                                 <  1:3624790/150‑1 (MQ=255)
                                                  gtgtTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGaa                                                                                               <  2:25221/150‑1 (MQ=255)
                                                        tacACGAAACGCCGTTGAGCAATCGACTCCGTATTTTTATAGCTTATTCTTATTAAATTCTCTTACACCGGACAATAAAAAATCCCGCCGCAGGCGTGATTTAAAGCAAGAGCAATCTACAAAAGATTATTGCGTGATCAGGAAAtcgtc                                                                                         >  2:3031010/3‑150 (MQ=255)
                                                        tCCTCGAATCGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCTGGAAAtcgtc                                                                                         <  2:2652945/150‑1 (MQ=255)
                                                          cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                       <  1:3542462/150‑1 (MQ=255)
                                                          cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTAGTATTAAATTGTATTAAACCGGACAATAAAAAAACCCCCCGCTGGCGGGATTTTAATCAAGTTCAAAAAACAAAAGATTATTTCTTGATAAGGAAATAGTCGa                                                                                       >  1:1059144/1‑150 (MQ=255)
                                                           aCGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAAATGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAg                                                                                      >  2:2816410/1‑150 (MQ=255)
                                                           aCGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCGTAGGCGTAGTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCGGGCGGGATTTTAAGCAAGTGCAATCTACCAAGGATTATTGCGTGATCAGGAACTCGTCGAg                                                                                      >  2:2505369/1‑150 (MQ=255)
                                                            cGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAgg                                                                                     <  1:278735/150‑1 (MQ=255)
                                                            cGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAgg                                                                                     <  1:1843368/150‑1 (MQ=255)
                                                            cGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAgg                                                                                     <  2:3549896/150‑1 (MQ=255)
                                                               aaCGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTTTATTAAAAAGGACAAAAAAAAAAAACCCCGCGGGCGGGATTTTTAGCAAGTGCAATATACAAAATATTATTTCTTGTTAAGGAAATCGTCGAGGGAt                                                                                  >  1:1106402/1‑150 (MQ=255)
                                                                  ggCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTa                                                                               >  2:3388725/1‑150 (MQ=255)
                                                                  ggCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTa                                                                               >  2:139675/1‑150 (MQ=255)
                                                                    cGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTAcc                                                                             <  2:495085/150‑1 (MQ=255)
                                                                    cGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTAcc                                                                             <  2:2692676/150‑1 (MQ=255)
                                                                         aGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAAATTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCt                                                                        <  1:1618783/150‑1 (MQ=255)
                                                                          gCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTc                                                                       >  2:2231087/1‑150 (MQ=255)
                                                                           cAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTATTAAAACGGACAAAAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCAACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCa                                                                      >  1:2685612/1‑150 (MQ=255)
                                                                             aTCGACGCCTTTTTTTTATAGCTTATTCTTATTAATTTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCTTc                                                                    <  2:2408468/150‑1 (MQ=255)
                                                                             aTCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATc                                                                    <  2:2408553/150‑1 (MQ=255)
                                                                               cGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATGTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCa                                                                  <  1:3686276/150‑1 (MQ=255)
                                                                                gACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCAt                                                                 <  2:1474654/150‑1 (MQ=255)
                                                                                gACGCCGTATTTTTATAGCTTATTCTTAGTAAAATGTCTTAAACCGGACAATAAAAAATCACGCATCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGATTGATCAGGAAATCGTCGAGGGATTTACCTTGCACATccca                                                                 >  2:2433039/1‑148 (MQ=255)
                                                                                         tttttATATCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCTCTGTCGTGACTTTAAGAAATTGCAATCTACAAATCATTATTTCTTTATCAGGAAATCGTAGAGGGATTTACCTTGCTCATCCATTGCtttttt                                                        <  1:4123344/150‑1 (MQ=255)
                                                                                            ataaaGCATATACTTAATAAATTGTCATAAACCCGACAATAAAAAATCCCGCCGCTGCCGGGATATTAAGCAACTGCAATCTACAAAACATTATTGCTTGATCACGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTATTTTGAt                                                     <  2:1879095/146‑1 (MQ=255)
                                                                                              ataGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTa                                                   <  1:456056/150‑1 (MQ=255)
                                                                                                aGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACa                                                 >  2:545854/1‑150 (MQ=255)
                                                                                                aGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACa                                                 >  1:818263/1‑150 (MQ=255)
                                                                                                   ttattcttattAAATTGTCTTACACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCt                                              <  2:2050401/150‑1 (MQ=255)
                                                                                                   ttattcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCt                                              <  2:901855/150‑1 (MQ=255)
                                                                                                      ttcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGa                                           <  1:2259047/150‑1 (MQ=255)
                                                                                                       tcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAg                                          >  1:790081/1‑150 (MQ=255)
                                                                                                         ttattaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTa                                        <  2:3281245/150‑1 (MQ=255)
                                                                                                         ttattaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTa                                        <  2:3281251/150‑1 (MQ=255)
                                                                                                           attaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACg                                      >  2:594902/1‑150 (MQ=255)
                                                                                                           attaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTGTGATTACAGCTGGAGTACg                                      >  1:2757556/1‑150 (MQ=255)
                                                                                                            ttaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACgg                                     >  1:3988004/1‑150 (MQ=255)
                                                                                                                aTTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCtt                                 <  2:3473222/150‑1 (MQ=255)
                                                                                                                   gTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGc                              <  2:2822570/150‑1 (MQ=255)
                                                                                                                    tCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGcc                             >  2:4013744/1‑150 (MQ=255)
                                                                                                                     cTTCAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCa                            <  1:1788593/150‑1 (MQ=255)
                                                                                                                     cTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCa                            <  1:3740258/150‑1 (MQ=255)
                                                                                                                     cTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCa                            <  2:727981/150‑1 (MQ=255)
                                                                                                                      ttAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTTATTTTTTGATTACAGCTGGAGTACTGCCTTGGCCAg                           >  2:2813150/1‑150 (MQ=255)
                                                                                                                      ttAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAg                           >  1:1044037/1‑150 (MQ=255)
                                                                                                                      ttAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAg                           >  2:3239737/1‑150 (MQ=255)
                                                                                                                      ttAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCAAg                           >  2:3900289/1‑150 (MQ=255)
                                                                                                                           ccGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCCTTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAg                      >  1:3237171/1‑150 (MQ=255)
                                                                                                                            cGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAgg                     >  1:2595347/1‑150 (MQ=255)
                                                                                                                            cGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAgg                     <  2:2788858/150‑1 (MQ=255)
                                                                                                                            cGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAgg                     >  1:681092/1‑150 (MQ=255)
                                                                                                                            cGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAgg                     >  1:717668/1‑150 (MQ=255)
                                                                                                                            cGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAgg                     >  1:3610701/1‑150 (MQ=255)
                                                                                                                            cGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAgg                     <  1:191946/150‑1 (MQ=255)
                                                                                                                               aCAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCGTGGCCAGTCCAGGttt                  >  1:2335822/1‑150 (MQ=255)
                                                                                                                                 actAAATAATCCCTCCGCTGGAGTGATTTTTAGCAAGTGCATTCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGTGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTa                <  2:1790240/148‑1 (MQ=255)
                                                                                                                                 aaTAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTa                <  1:3104281/150‑1 (MQ=255)
                                                                                                                                   tAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTTCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGt              >  1:2961639/1‑150 (MQ=255)
                                                                                                                                    ttaaaaTCCCGCCAGCGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTGTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGGCCAGGTGTTAGtt             >  1:2877394/3‑150 (MQ=255)
                                                                                                                                      aaaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTc           >  1:1256622/1‑150 (MQ=255)
                                                                                                                                      aaaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTc           >  1:2510774/1‑150 (MQ=255)
                                                                                                                                       aaaTCCCGCCGCTGGCGTGATTTTATTCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCg          <  1:3335393/150‑1 (MQ=255)
                                                                                                                                        aaTCCCGCCAGCGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGc         >  1:2241191/1‑150 (MQ=255)
                                                                                                                                         aTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGcc        >  2:16123/1‑150 (MQ=255)
                                                                                                                                         aTCCCGCCAGCGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGcc        >  1:206707/1‑150 (MQ=255)
                                                                                                                                              gccgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGtttt   >  2:3465323/1‑150 (MQ=255)
                                                                                                                                              gccgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGtttt   >  2:3321701/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTC  >  NC_000913/1292327‑1292619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑