Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,523 G→T 9.0% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5230GT 9.0% 431.9 / 7.6 161L72I (CTT→ATT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (85/56);  new base T (2/17);  total (88/73)
Fisher's exact test for biased strand distribution p-value = 3.82e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238374‑238672
                                                                                                                                                     |                                                                                                                                                     
  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                                     >  2:1653449/1‑150 (MQ=255)
  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                                     >  1:1963284/1‑150 (MQ=255)
  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                                     >  2:1709761/1‑150 (MQ=255)
  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATCGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                                     >  2:1709804/1‑150 (MQ=255)
   aTTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagtaa                                                                                                                                                    <  2:1626296/150‑1 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                                <  1:3856753/150‑1 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                                <  2:2975757/150‑1 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                                >  2:2239460/1‑150 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                                >  2:2239469/1‑150 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                                <  1:3542138/150‑1 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                                <  1:3542150/150‑1 (MQ=255)
            tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                                           >  2:168494/1‑150 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         >  1:280280/1‑150 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGaa                                                                                                                                         >  2:3712085/1‑150 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        >  2:124182/1‑150 (MQ=255)
                  gACAAAAGGAGCTATTAAAGGTGCTATTAGGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCAGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAtt                                                                                                                                     >  2:203477/1‑150 (MQ=255)
                    cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                   <  2:3912955/150‑1 (MQ=255)
                        aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  2:480233/150‑1 (MQ=255)
                           aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                                            >  2:3949658/1‑150 (MQ=255)
                            gCTATTATAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTATCTGTATTTTTATGTCTGTGTCGAAATTGAGTACCTTCGTTTTTTTGTTTATGAATGATTTTATTATAGTGAGTAAGATTAGTTGAATATTTAATATGGaa                                                                                                                           >  2:229664/1‑150 (MQ=255)
                            gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                           >  1:3126650/1‑150 (MQ=255)
                             cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGaaa                                                                                                                          >  2:3240837/1‑150 (MQ=255)
                             cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                          >  2:2420823/1‑150 (MQ=255)
                              tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                         >  2:2221672/1‑150 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                           <  2:3662963/147‑1 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                           >  1:3662963/1‑147 (MQ=255)
                                 tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGa                                                                                                                      >  2:2785166/1‑150 (MQ=255)
                                  aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                     <  2:2211496/150‑1 (MQ=255)
                                   aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                                    >  1:631034/1‑150 (MQ=255)
                                     ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACATg                                                                                                                  >  2:3562040/1‑150 (MQ=255)
                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                 >  2:1538591/1‑150 (MQ=255)
                                                  tAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                                     <  2:1843069/150‑1 (MQ=255)
                                                      tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                 >  2:1044995/1‑150 (MQ=255)
                                                       aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                >  1:2296124/1‑150 (MQ=255)
                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                           >  1:35129/1‑150 (MQ=255)
                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTTAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAAAt                                                                                           >  1:4099244/1‑150 (MQ=255)
                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAAATGAAAACGAATCACACAATCAACAt                                                                                           >  1:2973343/1‑150 (MQ=255)
                                                             aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATACATTAATATTTAAAAATGAAAAAGACATGAAACCGAAGCACAGAATCAACAtt                                                                                          >  1:3724112/1‑150 (MQ=255)
                                                                 atatAAGTTTTATCTTTTTTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGATTTATTTTATTATCGTAAGTAAAATTAAATGAATATTAAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                                      <  1:620401/150‑1 (MQ=255)
                                                                 atatAACAGTCACCTGTATTCTTATGTCTGTGGCCAAATTCACTACATACTATTTTTACATTAACAATCATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                                      <  2:3513333/150‑1 (MQ=255)
                                                                   ataataGTTAGTTTTATTTTTCTGTCTGTGCCTAAATTCACAACATTCTTTTTTTTTATTAATAATTATTTTATTATCTTAATAAAATTTAAATCAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                    <  1:3479471/150‑1 (MQ=255)
                                                                   ataAGATTTTGGTTTATTGTTTTGTGTGTGGCTAAATTGAATACCTTCGTTTTTTTTATAAATAATGTTTTTATTATCTTAATTAAATTTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                    <  1:3027409/150‑1 (MQ=255)
                                                                   ataACAGTTAGCTGTATTTTTATGTCCGTGGCCAAATTGACTCCCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGAAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                    <  2:2433349/150‑1 (MQ=255)
                                                                    taAGAGTTAGCTTTATTTTTATTTCTGTGGCGAATTTTACTACCTTCTTTTTTTTGATTAATAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                   <  2:1407625/150‑1 (MQ=255)
                                                                          ttAGCTGTTTTTTTATGTCTGTGGATAATTTGACTACATTGTTTTTTTTTTTTAATAATGATTTTTTTATCTTAATTAAAATTACATTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                             <  1:53275/150‑1 (MQ=255)
                                                                            aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATTATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAACAATCTCCCAAAATaaaaa                                                                           >  1:3580008/1‑149 (MQ=255)
                                                                            aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGTATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAATATGGTAAACGACATGAAACCGAAGCACAGAATCAACATTCTGCAATCATAatat                                                                           >  2:1294688/1‑150 (MQ=255)
                                                                              cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTTAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACAGAAGCACACAATAAAAATTATCCAAAAATAAAatat                                                                         >  1:1589193/1‑150 (MQ=255)
                                                                              cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                         >  2:1621000/1‑150 (MQ=255)
                                                                               tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTTATAATTATTTTATTATAGTAAGTAAAATTACATGAAAATTTAAAAATGAAAACGACATGAAACCGAATCACAGAATAAACATTCACCAAACAAAAAAAAtt                                                                        >  1:846018/1‑150 (MQ=255)
                                                                               tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAATACAAGAAAATTTAAAAAGGAAAACCACATGAAACCCAAGCACAGAAAAAACAATCACCAAACaaaaaaaaat                                                                        >  1:3747456/1‑146 (MQ=255)
                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                  <  2:1982760/150‑1 (MQ=255)
                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAATTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAATCAACAATAATAAAATAATTACGTg                                                                  >  1:2282745/1‑150 (MQ=255)
                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGAATTTATTATAGTAAATAAAAATAAAAGAATAATTAAAAATGAAAACGACAAGAAAACCAAGCACACAATAAACAATAAACAATAATAAAAAATTTACGTg                                                                  >  1:2282750/1‑150 (MQ=255)
                                                                                      ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATTATTTTATTATAGTAAGTAAAAATAAAAGAATATTTAAAAAGGAAAAAGAAATGAAAACCAAACACAGAATAAAAAATATCCAAAAATAAAATATTTAAGTgg                                                                 >  1:3231171/1‑150 (MQ=255)
                                                                                       ttggTCGGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAATATTGTTTTTATTATCGTAAGTAAAATTACTTTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                <  1:3205424/147‑1 (MQ=255)
                                                                                            atgtgGCTAAATTGAATACCTTCTTTTTTTTGATTAATAATGATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                           <  2:8189/149‑1 (MQ=255)
                                                                                                 gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                      >  2:3454211/1‑150 (MQ=255)
                                                                                                 gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCCACGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttctt                                                      >  2:2108171/1‑150 (MQ=255)
                                                                                                   gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                    <  2:1974280/150‑1 (MQ=255)
                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  2:1268498/150‑1 (MQ=255)
                                                                                                    aaaTAGACTACCAAACTTTTTTTAATAAACAATCATTTTATTATCTAAATTAAAATTAAATCAAAATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  2:766568/150‑1 (MQ=255)
                                                                                                           cTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                            <  1:1538591/150‑1 (MQ=255)
                                                                                                             aCCTTCGTTTTTTTTATTAAGACTGATTGTTTTATCGTACGTAAATTTACATGAATATTTAAAACGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                          <  2:3112513/150‑1 (MQ=255)
                                                                                                              ccTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  1:978202/150‑1 (MQ=255)
                                                                                                              ccTTCGTTTTTTTGTTTAATAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCTTAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  1:978014/150‑1 (MQ=255)
                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  2:614838/150‑1 (MQ=255)
                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  2:519036/150‑1 (MQ=255)
                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                       >  2:3111244/1‑150 (MQ=255)
                                                                                                                 tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGtt                                      <  2:3184893/150‑1 (MQ=255)
                                                                                                                  cGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                     <  1:765477/150‑1 (MQ=255)
                                                                                                                     ttttttTATTAATAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  2:3588129/150‑1 (MQ=255)
                                                                                                                     ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  1:176368/150‑1 (MQ=255)
                                                                                                                     ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  1:1068825/150‑1 (MQ=255)
                                                                                                                       ttttTATTAATAATTATTTTATTATCTTAATTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                <  1:2186583/150‑1 (MQ=255)
                                                                                                                       ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                <  2:266023/150‑1 (MQ=255)
                                                                                                                        tttttttAATAATGATTTTATTATCGTATGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGATACCGAAGCACAGAATCAACATTCTCCAACCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               <  2:2582702/145‑1 (MQ=255)
                                                                                                                        tttGATTAAGAATTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               <  2:2440754/150‑1 (MQ=255)
                                                                                                                        tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               <  1:3009645/150‑1 (MQ=255)
                                                                                                                         ttGATTAAGAATGATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                              <  2:1101059/150‑1 (MQ=255)
                                                                                                                         ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                              <  2:284947/150‑1 (MQ=255)
                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           >  1:2987742/1‑150 (MQ=255)
                                                                                                                             ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                          >  2:373323/1‑150 (MQ=255)
                                                                                                                             ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                          >  2:3488939/1‑150 (MQ=255)
                                                                                                                             ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATCGAATATAGAGGTTTAACTCCGGTaa                          >  2:1815010/1‑150 (MQ=255)
                                                                                                                              tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                         >  1:2096147/1‑150 (MQ=255)
                                                                                                                              tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                         >  1:145232/1‑150 (MQ=255)
                                                                                                                              tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                         >  1:480010/1‑150 (MQ=255)
                                                                                                                               aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                        >  1:1080503/1‑150 (MQ=255)
                                                                                                                                    tgtttttATTTTCGTACGTAAAATTACATGAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa                   <  2:3455136/147‑1 (MQ=255)
                                                                                                                                     taTTTTATTATCGTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  2:2987738/149‑1 (MQ=255)
                                                                                                                                     gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  2:431888/150‑1 (MQ=255)
                                                                                                                                        tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGACAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa               >  1:2436959/1‑150 (MQ=255)
                                                                                                                                        tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa               >  1:2523130/1‑150 (MQ=255)
                                                                                                                                        tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa               >  2:3023026/1‑150 (MQ=255)
                                                                                                                                        tttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa               >  2:2157453/1‑150 (MQ=255)
                                                                                                                                             tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          <  2:3724129/150‑1 (MQ=255)
                                                                                                                                                cGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa       <  1:3152318/150‑1 (MQ=255)
                                                                                                                                                 gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt      >  2:3163649/1‑150 (MQ=255)
                                                                                                                                                 gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt      >  1:2570924/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238374‑238672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: