Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,906,608 G→C 5.7% intergenic (+25/+35) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,906,6080GC 5.7% 996.3 / 40.2 277intergenic (+25/+35)ygcG/enoTPM domain protein, putative phosphatase/enolase
Reads supporting (aligned to +/- strand):  ref base G (133/128);  new base C (9/7);  total (142/135)
Fisher's exact test for biased strand distribution p-value = 7.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 286 total aligned reads displayed.

AGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAA  >  NC_000913/2906459‑2906757
                                                                                                                                                     |                                                                                                                                                     
aGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCg                                                                                                                                                       <  2:3582706/150‑1 (MQ=255)
  aaaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGa                                                                                                                                                     >  1:3835087/1‑150 (MQ=255)
         cATCTGGTAGAAGTTATCATTCAGACCACAGCGGGTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGc                                                                                                                                              <  2:3682516/150‑1 (MQ=255)
         cATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGc                                                                                                                                              <  2:925369/150‑1 (MQ=255)
          aTCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCa                                                                                                                                             <  2:1607438/150‑1 (MQ=255)
          aTCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCa                                                                                                                                             >  1:1674683/1‑150 (MQ=255)
           tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAt                                                                                                                                            >  1:3255824/1‑150 (MQ=255)
             tGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAttt                                                                                                                                          <  1:871860/150‑1 (MQ=255)
             tGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttt                                                                                                                                          >  2:2493018/1‑150 (MQ=255)
             tGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttt                                                                                                                                          >  2:2051821/1‑150 (MQ=255)
             tGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttt                                                                                                                                          >  2:1010867/1‑150 (MQ=255)
               gTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttttt                                                                                                                                        >  1:2013172/1‑150 (MQ=255)
                tAGAAGTTATCATTGAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTa                                                                                                                                       >  1:2488434/1‑150 (MQ=255)
                tAGAAGTTATCATTCGGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTa                                                                                                                                       >  1:405731/1‑150 (MQ=255)
                 aGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTaa                                                                                                                                      >  1:2695689/1‑150 (MQ=255)
                  gAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTaaa                                                                                                                                     <  2:1626466/150‑1 (MQ=255)
                   aaGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAAt                                                                                                                                    >  1:2241622/1‑150 (MQ=255)
                     gTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCa                                                                                                                                  <  1:3178797/150‑1 (MQ=255)
                      ttATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCCACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAg                                                                                                                                 >  1:4074738/1‑150 (MQ=255)
                      ttATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAg                                                                                                                                 >  1:547738/1‑150 (MQ=255)
                      ttATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAg                                                                                                                                 >  1:133553/1‑150 (MQ=255)
                           aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGTGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaaa                                                                                                                            >  2:2909500/1‑150 (MQ=255)
                           aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaaa                                                                                                                            <  1:686279/150‑1 (MQ=255)
                           aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaaa                                                                                                                            <  2:4058051/150‑1 (MQ=255)
                           aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaaa                                                                                                                            <  1:2359094/150‑1 (MQ=255)
                            ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAag                                                                                                                           >  2:1530093/1‑150 (MQ=255)
                            ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAag                                                                                                                           >  2:417420/1‑150 (MQ=255)
                            ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAag                                                                                                                           >  1:3927976/1‑150 (MQ=255)
                                gACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtcag                                                                                                                       >  1:1469182/1‑150 (MQ=255)
                                      aGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTAt                                                                                                                 >  1:1709103/1‑150 (MQ=255)
                                         ggTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATTCCAGCCCGGAGGCTGGCATTTTTAAATAAGATAAAGTAAGTCTTATgac                                                                                                              >  1:1424087/1‑148 (MQ=255)
                                               gCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCt                                                                                                        >  1:3593092/1‑150 (MQ=255)
                                                 tGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCttt                                                                                                      <  1:751935/150‑1 (MQ=255)
                                                 tGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCttt                                                                                                      <  2:2791220/150‑1 (MQ=255)
                                                 tGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCttt                                                                                                      <  1:3727716/150‑1 (MQ=255)
                                                 tGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCttt                                                                                                      <  1:2906937/150‑1 (MQ=255)
                                                    aGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGAt                                                                                                   <  2:1838573/150‑1 (MQ=255)
                                                     ggCTCAGATAGCGGCGGATGTCGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCGGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATCAAGTCAGTCTTAGGCCTGGCCTTTGAtc                                                                                                  <  2:3459257/150‑1 (MQ=255)
                                                        tCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATctct                                                                                               >  2:694664/1‑150 (MQ=255)
                                                        tCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATctct                                                                                               >  2:3493113/1‑150 (MQ=255)
                                                        tCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATctct                                                                                               <  2:4104841/150‑1 (MQ=255)
                                                         cAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCGGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCtt                                                                                              <  2:50911/150‑1 (MQ=255)
                                                          aGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCttt                                                                                             <  2:3906880/150‑1 (MQ=255)
                                                           gATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTa                                                                                            <  1:1252733/150‑1 (MQ=255)
                                                                cggcggATTTTGCGGCGGGTGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGAcc                                                                                       <  1:2675788/150‑1 (MQ=255)
                                                                       tttAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAATTCAGTCTTATGCCTGGCCTTTGATCTCTTTAAGACCGTTGTAc                                                                                >  1:3386513/1‑150 (MQ=255)
                                                                       tttAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTAc                                                                                >  1:3657838/1‑150 (MQ=255)
                                                                           gcggcgGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTg                                                                            >  2:3743361/1‑150 (MQ=255)
                                                                               cggGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCttt                                                                        <  2:1342524/150‑1 (MQ=255)
                                                                                 ggggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAAACAAAAAAAATGACAGCCCCGAGGCTGGCATTTTTAAATAAGATAAAGTAAGTCTTATTCCTGGCCTTTTATATCTTTAAGACCGTTGTAAGGTGCttttt                                                                      >  1:820020/1‑150 (MQ=255)
                                                                                  gggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTc                                                                     <  2:3297677/150‑1 (MQ=255)
                                                                                   ggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTct                                                                    <  2:2015959/150‑2 (MQ=255)
                                                                                   ggCGGTTCTTCTGGAGGTGGCGGCGCAACCGGCCGCTGGTAACTCAACCAAAAAAAATTACAGCCCGGAGGCTGGCATTTTTAAATAAGATAAAGTAAGTATTATGCCTTGCCTTTGATCTCTTTACGACCGTTTTAAGGTGCtttttag                                                                    >  1:1088639/1‑148 (MQ=255)
                                                                                     cGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGcc                                                                  <  1:417420/150‑1 (MQ=255)
                                                                                       gTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCa                                                                <  2:245778/150‑1 (MQ=255)
                                                                                        ttcttcTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCag                                                               >  1:3905276/1‑150 (MQ=255)
                                                                                         tcttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCaga                                                              <  2:2367868/150‑1 (MQ=255)
                                                                                         tcttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCaga                                                              <  1:1879713/150‑1 (MQ=255)
                                                                                               ggAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGcttct                                                        <  1:2570022/150‑1 (MQ=255)
                                                                                                gAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGTCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGcttctt                                                       <  2:3017288/150‑1 (MQ=255)
                                                                                                gAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGcttctt                                                       <  2:4005440/150‑1 (MQ=255)
                                                                                                 aGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCttcttc                                                      >  2:2724407/1‑150 (MQ=255)
                                                                                                   gTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGa                                                    <  1:320995/150‑1 (MQ=255)
                                                                                                    tGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGAt                                                   <  1:2981686/150‑1 (MQ=255)
                                                                                                      gcggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATAc                                                 <  2:2818983/150‑1 (MQ=255)
                                                                                                       cggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGCCCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACg                                                >  1:245098/1‑150 (MQ=255)
                                                                                                           gcATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATc                                            <  1:2180038/150‑1 (MQ=255)
                                                                                                               ccGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCAGGCCTTTTATCTCTTTAAGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCt                                        >  1:634846/1‑150 (MQ=255)
                                                                                                                cGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTg                                       >  2:1664953/1‑150 (MQ=255)
                                                                                                                 ggCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTgg                                      >  2:2276903/1‑150 (MQ=255)
                                                                                                                  gCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGt                                     >  1:2281064/1‑150 (MQ=255)
                                                                                                                    cGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTg                                   <  2:3111670/150‑1 (MQ=255)
                                                                                                                     gCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGt                                  >  1:1509002/1‑150 (MQ=255)
                                                                                                                       tGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTAt                                >  1:773826/1‑150 (MQ=255)
                                                                                                                       tGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTAt                                >  1:774218/1‑150 (MQ=255)
                                                                                                                           aaCTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAg                            <  2:3271884/150‑1 (MQ=255)
                                                                                                                              tCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGACTCAGCTGGTTGTAGTTAGCaa                         >  2:2416223/1‑150 (MQ=255)
                                                                                                                              tCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGACTCAGCTGGGTGTCTTTAGCaa                         >  2:883814/1‑150 (MQ=255)
                                                                                                                              tCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCaa                         >  2:1743463/1‑150 (MQ=255)
                                                                                                                              tCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCaa                         <  1:1010867/150‑1 (MQ=255)
                                                                                                                              tCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCaa                         <  1:1621429/150‑1 (MQ=255)
                                                                                                                              tCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCGTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCaa                         >  2:3420389/1‑150 (MQ=255)
                                                                                                                                 aCCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAacac                      <  1:2842313/150‑1 (MQ=255)
                                                                                                                                    aTAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt                   <  1:1042483/150‑1 (MQ=255)
                                                                                                                                    aTAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt                   >  1:3496144/1‑150 (MQ=255)
                                                                                                                                     tacaaaaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGGTGGTTGTATTTAGCAACACGGTc                  <  2:3799958/147‑1 (MQ=255)
                                                                                                                                     tAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTc                  >  1:498848/1‑150 (MQ=255)
                                                                                                                                     tAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTc                  <  2:3934763/150‑1 (MQ=255)
                                                                                                                                         aaaTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATCCGAATCAGCTGGTTGTATTTAGCACCACGGTCAGac              >  2:574833/1‑149 (MQ=255)
                                                                                                                                         aaaTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGACTCAGCGGGGTGTATTTAGCAACACGGTCAGaa              >  1:3344938/1‑150 (MQ=255)
                                                                                                                                         aaaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATCACGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGaa              <  2:1219159/150‑1 (MQ=255)
                                                                                                                                          aaTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAAc             >  1:3448549/1‑150 (MQ=255)
                                                                                                                                          aaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAAc             <  1:2602595/150‑1 (MQ=255)
                                                                                                                                           aTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACg            >  1:3698677/1‑150 (MQ=255)
                                                                                                                                           aTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCATTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACg            >  1:311752/1‑150 (MQ=255)
                                                                                                                                                aGCCCGGAGGCTGGAATTTTTAAATCAGATAAAGTCAGTCTTAGGCCGGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCTGCTGGTTTTATTTAGCAACACGGTCAGAACGGCTCa       <  1:2802049/150‑1 (MQ=255)
                                                                                                                                                    cGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGa   <  2:3941263/150‑1 (MQ=255)
                                                                                                                                                    cGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGa   >  2:2190388/1‑150 (MQ=255)
                                                                                                                                                    cGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGACTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGa   <  2:1903988/150‑1 (MQ=255)
                                                                                                                                                     ggAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCTGGTGGTTGTATTTAGCAACTCGGTCAGAACGGCTCATAGaa  >  2:594188/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGAA  >  NC_000913/2906459‑2906757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: