Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,081 G→T 5.3% intergenic (+479/+135) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,0810GT 5.3% 621.5 / 23.2 209intergenic (+479/+135)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base G (108/90);  new base T (8/3);  total (116/93)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

Only 100 of 222 total aligned reads displayed.

TTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCG  >  NC_000913/3269932‑3270230
                                                                                                                                                     |                                                                                                                                                     
  catCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGACGCTTTCTTTTGGGTATAGTGTCTTTGAAAGTCATTCATGATTCTTCACCTACAAAAGCAAAAACAAGCCGaa                                                                                                                                                     >  2:2139004/1‑150 (MQ=11)
      tttCTGCCCCTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                                 <  1:2115512/150‑1 (MQ=37)
      tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                                 <  1:1249906/150‑1 (MQ=37)
      tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCATATTTCAGCCCCTCCAAAAGCAAAAACCCGCCGCAGCgg                                                                                                                                                 >  2:1106448/1‑150 (MQ=255)
           ggcccTCCAAAAGTATAAACCCGCCGTAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                                                                            <  2:2275109/148‑1 (MQ=255)
            ccccTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                                           <  2:1540086/150‑1 (MQ=17)
            ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                                           >  2:2061106/1‑150 (MQ=37)
              ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTAGAGTGTCGTGGACAGTCATTCAACGTTGTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGgtttttc                                                                                                                                         >  1:4184/1‑148 (MQ=11)
                   aaaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCGTTCAGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                                    >  2:369754/1‑150 (MQ=255)
                   aaaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGGATCGTGTCGTGGCCCGTCAGTCATCTGTCTGCCCCGCCCAAAGCAAAAACCCGCCGAAGCGGGGTGTTACGTtaa                                                                                                                                    >  1:807426/1‑150 (MQ=11)
                   aaaaGTAAAAACCCGCCGAAGCGGGTTTTTACGAAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCATTCTCCCCCTCCAAAAGCAATAACCCGCCGAAGCGGGGTGGTACGTaaa                                                                                                                                    >  2:3293358/1‑150 (MQ=11)
                      aGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa                                                                                                                                 <  2:3483098/150‑1 (MQ=39)
                       gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                                                                <  1:844358/150‑1 (MQ=38)
                       gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAg                                                                                                                                >  2:1720386/1‑150 (MQ=16)
                        tAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgg                                                                                                                               <  2:142612/150‑1 (MQ=38)
                        tAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTCATTCATATTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTCAAACAgg                                                                                                                               >  1:702852/1‑150 (MQ=11)
                         aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                                                                                                                              <  1:1716306/150‑1 (MQ=37)
                         aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGt                                                                                                                              <  1:1001701/150‑1 (MQ=37)
                          aaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTTCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAgggg                                                                                                                             >  1:4073620/1‑148 (MQ=37)
                                cgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTg                                                                                                                       >  1:1629250/1‑150 (MQ=255)
                                cgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACGTTCGGCCCCTCCAAAACCAAAAACCCGCCGAAGCGGGGTTTTACGTAAACCAGGTGAAACTg                                                                                                                       >  2:1941898/1‑150 (MQ=11)
                                  ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                     <  1:3029996/150‑1 (MQ=37)
                                       gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                                <  1:2877647/150‑1 (MQ=37)
                                         gggTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg                                                                                                              <  2:2563824/150‑1 (MQ=37)
                                          ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                                                             <  1:3330280/150‑1 (MQ=37)
                                          ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                                                             <  2:3955778/150‑1 (MQ=37)
                                             ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                          <  1:449050/150‑1 (MQ=38)
                                                   gTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttctt                                                                                                    >  2:637494/1‑150 (MQ=255)
                                                     acatcAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                  <  1:2853538/146‑1 (MQ=18)
                                                     aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                  <  1:1808013/150‑1 (MQ=40)
                                                      catcAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                 <  2:2738658/147‑1 (MQ=12)
                                                      aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                 >  2:2515227/1‑150 (MQ=39)
                                                       aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                                <  2:900366/150‑1 (MQ=38)
                                                            gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGgata                                                                                           >  2:3477507/1‑150 (MQ=255)
                                                                   tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATAATCTTTATGCCCCTCCAAAAGCAAAAACCCCCCGAAGCCGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGCTTTCTTTTGGGGATAGcgtcgt                                                                                    >  1:2477410/1‑150 (MQ=11)
                                                                       cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTAAAGCGACGTGGAc                                                                                >  2:457708/1‑150 (MQ=11)
                                                                         aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAg                                                                              <  1:3409934/150‑1 (MQ=37)
                                                                          tAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATAAAAATTATTCCCCAACAAAATCAAAAAACCCCCGAAGCCGGTTTTTTCGTAAAACAAGTGGAACAGAACGATAATCCCCATTAATTTTGGTATATCGGCGTGGACaat                                                                             >  1:3853051/1‑148 (MQ=2)
                                                                                  tgtctttTTGGTCTTGTGTCGTGGACTGTGAGTAGTCTTACTGGCACTCCAAAATCATAAACCCGCCGTAGCGGTTTATTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATc                                                                     <  2:2388173/148‑1 (MQ=11)
                                                                                   ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCt                                                                    <  1:2394496/150‑1 (MQ=37)
                                                                                   ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAATCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAAAAGACCGATAATCCGCATTATTTTGGGGATAGCGTCGTGGACAGtcaataataa                                                                    >  1:1670380/1‑143 (MQ=11)
                                                                                           ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                            <  2:4070057/150‑1 (MQ=37)
                                                                                              ttaGTGTCGTGGACAGTGTTTCTTCTTTCTGCCCCTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                         <  2:2229341/149‑1 (MQ=255)
                                                                                              ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                         <  1:3924145/150‑1 (MQ=37)
                                                                                              ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                                         <  2:2846381/150‑1 (MQ=37)
                                                                                                   gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                    <  1:3317473/150‑1 (MQ=37)
                                                                                                    tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                                   >  1:3957958/1‑150 (MQ=37)
                                                                                                      gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGATAGCGTCGTGGACAGGCATTCATCGTTCTGCCCCGCCAAAAGCa                                                 >  2:2636910/1‑150 (MQ=11)
                                                                                                      gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGACTCTATCTTTAGGGTATAGCGACTTGGACAGAAATTCAAAAGTCAGCCCCACCAAAATCa                                                 >  2:2636885/1‑150 (MQ=11)
                                                                                                       tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCGCCTCCAAAATCaa                                                >  1:2563615/1‑150 (MQ=255)
                                                                                                       tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                                >  1:482519/1‑150 (MQ=37)
                                                                                                       tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCaa                                                >  1:3433955/1‑150 (MQ=37)
                                                                                                          aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                             >  2:641089/1‑150 (MQ=37)
                                                                                                             gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGAGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                                          <  2:1853033/150‑1 (MQ=255)
                                                                                                              tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                         <  1:3439853/150‑1 (MQ=37)
                                                                                                               ctctcatcaGTCGGCCCCTCCAAACGCTAAAACCCGCCGTATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                        <  2:4066001/147‑1 (MQ=11)
                                                                                                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACccgccg                                      >  2:515130/1‑150 (MQ=37)
                                                                                                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                      >  2:1202894/1‑150 (MQ=37)
                                                                                                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                      >  2:3241306/1‑150 (MQ=37)
                                                                                                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                      >  1:2003513/1‑150 (MQ=37)
                                                                                                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCATCCCCTCCAAAAGCAAAAAAccgccg                                      >  2:1204781/1‑150 (MQ=255)
                                                                                                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTCATCGTGCTGCCCCTCCAAAATCAAAAACccgccc                                      >  2:3816415/1‑149 (MQ=255)
                                                                                                                 ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTACAAAAGCAAAATCccgccg                                      >  2:3034926/1‑150 (MQ=255)
                                                                                                                  tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTGTCGGCCCCGGCAAAAACAAAAACCCGCCGa                                     >  2:879073/1‑150 (MQ=11)
                                                                                                                  tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTTCCCCTCCAAAAGCAAAAACCCGCCGa                                     >  1:1424443/1‑150 (MQ=255)
                                                                                                                    atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGACGTGTAAAGTCAATCATATTTAACCACCAACAATAGCAAAAACCCGCCTAAg                                   >  2:1621412/1‑150 (MQ=11)
                                                                                                                            gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGACGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttg                           >  1:2697445/1‑149 (MQ=255)
                                                                                                                             ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                          >  1:2183844/1‑150 (MQ=37)
                                                                                                                             ccccACCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt                          <  1:3511461/150‑1 (MQ=11)
                                                                                                                                 tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                      >  1:3703734/1‑150 (MQ=37)
                                                                                                                                 tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTaagg                      >  2:1827629/1‑147 (MQ=255)
                                                                                                                                 tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                      >  2:1144333/1‑150 (MQ=37)
                                                                                                                                  ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                     >  1:1706661/1‑150 (MQ=37)
                                                                                                                                  ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTa                     >  1:4005794/1‑150 (MQ=255)
                                                                                                                                   cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                    >  1:2345149/1‑150 (MQ=37)
                                                                                                                                   cAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCGGGGGGGTATAGCGTCGTGGACAGGCAGTCATCGTTCTGCCCCGCCAAAAGCCAAAACCCGCCGCAGCGGGTTTTTACggac                    >  2:1332611/1‑147 (MQ=11)
                                                                                                                                    aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTGGTTACGGaaa                   >  2:2466660/1‑150 (MQ=11)
                                                                                                                                      aaGCACAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATc                 <  2:1004444/150‑1 (MQ=255)
                                                                                                                                      aaGCAAAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATc                 <  2:1742641/150‑1 (MQ=255)
                                                                                                                                       atcAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                <  1:3783041/148‑1 (MQ=38)
                                                                                                                                       aGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                <  2:3423328/150‑1 (MQ=12)
                                                                                                                                       aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGAGACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACCGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                <  2:3423193/150‑1 (MQ=255)
                                                                                                                                         taaaaaCCCGCTTCGGCGGGTTTTTGCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt                                                                >  2:2276073/2‑100 (MQ=11)
                                                                                                                                         taaaaaCCCGCTTCGGCGGGTTTTTGCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt                                                                <  1:2275631/99‑1 (MQ=11)
                                                                                                                                         cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTATATCAgg              <  1:3417181/150‑1 (MQ=38)
                                                                                                                                          acaaaCCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt             <  1:420991/148‑1 (MQ=12)
                                                                                                                                          acaaaCCCGCCGAAGCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAACAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt             <  2:3858752/148‑1 (MQ=255)
                                                                                                                                          aaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt             >  2:3343289/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt             >  1:1654654/1‑150 (MQ=11)
                                                                                                                                          aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAATCAGGt             >  1:1857333/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCCTCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGGTTTTACGTAAATCAGGt             >  1:1322554/1‑150 (MQ=255)
                                                                                                                                              aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa         <  2:1629250/150‑1 (MQ=37)
                                                                                                                                               cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAc        >  1:3631551/1‑150 (MQ=37)
                                                                                                                                                ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt       >  1:3736292/1‑150 (MQ=37)
                                                                                                                                                ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt       >  1:1278132/1‑150 (MQ=37)
                                                                                                                                                 cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg      >  1:3075923/1‑150 (MQ=37)
                                                                                                                                                  gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                    <  1:3191254/103‑1 (MQ=35)
                                                                                                                                                  gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                    >  2:3191254/1‑103 (MQ=35)
                                                                                                                                                  gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa     >  2:3693262/1‑150 (MQ=37)
                                                                                                                                                   ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc    <  2:3374303/150‑1 (MQ=17)
                                                                                                                                                     |                                                                                                                                                     
TTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCG  >  NC_000913/3269932‑3270230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: