Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,221 T→C 7.1% L132P (CTG→CCG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,2210TC 7.1% 555.5 / 23.4 184L132P (CTG→CCG) ‡yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base T (73/93);  new base C (5/8);  total (80/104)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

Only 100 of 187 total aligned reads displayed.

TCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTT  >  NC_000913/3368073‑3368370
                                                                                                                                                     |                                                                                                                                                     
  tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTGTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                                    <  1:1754549/150‑1 (MQ=255)
  tGCAAAAAACTTACGCCAAATGAGGATTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                                    <  1:1199108/150‑1 (MQ=255)
   gCAAACAACTTACGCCAAAGGAGGCTTTTTTACCTCTTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                                    <  2:1510204/150‑1 (MQ=255)
   gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                                                                   <  2:2582732/150‑1 (MQ=255)
   gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                                                                   <  1:2106319/150‑1 (MQ=255)
     aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGGTTTGGCAGACGCTGTCTGc                                                                                                                                                 >  1:3920429/1‑150 (MQ=255)
      aaaaaaTTACGACAAATGTGTCTTTTTTACCTC‑TTTTTTAAACTTGTTTAAATTCACCAGACAATGTCTGGCTAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCTTCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                                                                <  1:50214/150‑1 (MQ=255)
      aaaaaCTTACGCCAAATGTGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                                                                <  2:3267261/150‑1 (MQ=255)
        aaaCTTACGCCAAATGAGGCTTTTTTTCCTC‑TTTGTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCaa                                                                                                                                              <  2:817170/150‑1 (MQ=255)
         aaattACGCCAAATGAGGCTTTTTTACCTA‑TTTTTTCAAATTGTTGAAATTCACAAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCATAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaaa                                                                                                                                             <  1:1658033/147‑1 (MQ=255)
         aaCTTACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATGGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                                                             <  1:1061567/150‑1 (MQ=255)
            ttACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCt                                                                                                                                          >  2:2990372/1‑150 (MQ=255)
              aCGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCttt                                                                                                                                        <  2:649882/150‑1 (MQ=255)
              aCGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCttt                                                                                                                                        <  2:1417468/150‑1 (MQ=255)
               cGCCCACTGAGGCTTTCTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGACTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTc                                                                                                                                       <  2:1195545/150‑1 (MQ=255)
               cGCCAAATGTGGCTTTTTTACCTA‑TTTTTTCAACTTTTTGAATTTCACCAGACAATGTCTGGCGAATTAACACCTTTGGTAATTCCGCAGACACCGTCTGAAGATTTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCAAATCtttg                                                                                                                                       <  1:3039338/150‑2 (MQ=255)
               cGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCAAATCtttg                                                                                                                                       <  2:2053304/150‑2 (MQ=255)
                 ccAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTccc                                                                                                                                     <  2:2980895/150‑1 (MQ=255)
                 ccAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAAGGAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGGCTGCCCAATCTTTccc                                                                                                                                     >  2:3587077/1‑150 (MQ=255)
                     aTGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTg                                                                                                                                 <  2:3518829/150‑1 (MQ=255)
                        aGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGccc                                                                                                                              <  2:939257/150‑1 (MQ=255)
                           cTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAACATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAgg                                                                                                                           >  1:1531615/1‑150 (MQ=255)
                            ttttttACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAggg                                                                                                                          >  1:135464/1‑149 (MQ=255)
                            ttttttACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGt                                                                                                                          <  2:2360106/150‑1 (MQ=255)
                              ttttACCAC‑TATGTTCACCAAGTTGCAATACACCAGACAAGGTCTGGCGAATTAACCCCATTGGGAATTCCGCAGACACCGTCTGCAGATTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTcc                                                                                                                        <  1:2769161/150‑1 (MQ=255)
                                 tACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAcc                                                                                                                     >  1:1844759/1‑150 (MQ=255)
                                 tACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAcc                                                                                                                     >  1:4077147/1‑150 (MQ=255)
                                    ctc‑tTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                                                  <  2:3244851/150‑1 (MQ=255)
                                    ctc‑tTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGCCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                                                  <  2:3300643/150‑1 (MQ=255)
                                     tc‑tTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                                 >  1:1002427/1‑150 (MQ=255)
                                     tc‑tTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTACCCTTGCCCAGGTCCACCTACg                                                                                                                 >  1:1070732/1‑150 (MQ=255)
                                         tttttCAACATGTTGAAATTCACCAGCCATTGTCTGGCGATTTAACACCATTGGGAATTCCGCAGACACCGTCTGCTGATTTCCCTTCGGCAAATATTTGGCAGACGCTTTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTcc                                                                                                              <  1:1035609/150‑1 (MQ=255)
                                         tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTACCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTcc                                                                                                              <  1:387286/150‑1 (MQ=255)
                                           tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGt                                                                                                            >  1:704418/1‑150 (MQ=255)
                                              cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATAGGCAAAGATTTGTCAGACGCGGTCTGCCAAATATTTCCCCTTTCCCAGGTCAACCTACGTCCGTTTa                                                                                                         >  1:3530655/1‑150 (MQ=255)
                                              cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAAGTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTa                                                                                                         <  2:3884727/150‑1 (MQ=255)
                                                aCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACt                                                                                                       >  2:3672907/1‑150 (MQ=255)
                                                   tGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCTTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                                                                    <  2:2410653/150‑1 (MQ=255)
                                                   tGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                                                                    <  1:3312000/150‑1 (MQ=255)
                                                   tGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                                                                                    <  2:2410608/150‑1 (MQ=255)
                                                     ttGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                                                                  <  2:1054383/150‑1 (MQ=255)
                                                          aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                             >  1:1629306/1‑150 (MQ=255)
                                                          aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                             >  1:2527012/1‑150 (MQ=255)
                                                          aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                             <  1:2533072/150‑1 (MQ=255)
                                                           ttCGCCAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaa                                                                                            >  1:3752269/1‑150 (MQ=255)
                                                             cgccAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGTCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAc                                                                                          >  2:1254057/3‑150 (MQ=255)
                                                               ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                        >  1:3741967/1‑150 (MQ=255)
                                                               ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                        <  2:3965755/150‑1 (MQ=255)
                                                               ccAGACAATGTCAGGCGAATTAACACCATAGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                        >  2:3345826/1‑150 (MQ=255)
                                                                        gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCg                                                                               >  1:3772560/1‑150 (MQ=255)
                                                                            ggCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGc                                                                           <  2:3910513/150‑1 (MQ=255)
                                                                             gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTATGCCAAATATTTACCCAGGCCCCGGGCCACCGACCTCCGGTTACTTTCCGTTAAAAAAGCAGACGCCcgccgcg                                                                          >  1:1954942/1‑149 (MQ=255)
                                                                             gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                                                                          <  2:3455749/150‑1 (MQ=255)
                                                                             gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAAGCTGACGCCCGCAGCt                                                                          >  2:2203270/1‑150 (MQ=255)
                                                                             gCGAATTAACACCATTGGGAAGTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATGTGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTACAAACGCTGACGCCCGCAGCt                                                                          <  2:2132123/150‑1 (MQ=255)
                                                                              cGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGGTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCtt                                                                         >  2:28037/1‑150 (MQ=255)
                                                                                 aTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                                                                      <  2:3826029/150‑1 (MQ=255)
                                                                                  ttAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAt                                                                     <  1:2582845/150‑1 (MQ=255)
                                                                                  ttAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAACGGCAAAGATTTGGCAGAAGCTGTCAGCCAAATCATTACCCATGCCCACGTCCACCAACGACCCGTTACATTACGTTAAAAACGCAGACGCCCGCAGCTTTTAt                                                                     >  1:3488862/1‑150 (MQ=255)
                                                                                      cacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                                 >  2:3405617/1‑150 (MQ=255)
                                                                                      cacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                                 <  1:899388/150‑1 (MQ=255)
                                                                                         cATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAAAGCAGACGCCCGCCGCTTTTATGaaaaat                                                              >  1:3664849/1‑149 (MQ=255)
                                                                                          cttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCACAGATTGGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                                                             <  1:3090176/149‑1 (MQ=255)
                                                                                          aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                                                             <  1:2674771/150‑1 (MQ=255)
                                                                                          aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                                                             <  1:3745279/150‑1 (MQ=255)
                                                                                           ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCAAAAAATTACCCTTTCCCAGGGCCACCAACCTCCCTTTAATTTACGTTAAAAAACCAGACGCCCCCAGCTTTTATGAAAAagag                                                            >  1:570996/1‑150 (MQ=255)
                                                                                           ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCCGCTTTTAAGAAAAagag                                                            >  2:1335571/1‑150 (MQ=255)
                                                                                            tGGGAAGTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAgaga                                                           <  1:4118046/150‑1 (MQ=255)
                                                                                             gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAGGAAAAAGAGAc                                                          >  2:2547521/1‑150 (MQ=255)
                                                                                                aaTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCt                                                       >  2:1228625/1‑150 (MQ=255)
                                                                                                    ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGc                                                   >  2:2746693/1‑150 (MQ=255)
                                                                                                     cGCAGACACGGTCTGCAGAATTCCCATCGGCAAAGATTGGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                                                  <  1:2821842/150‑1 (MQ=255)
                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAACGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                                                  <  1:2177474/150‑1 (MQ=255)
                                                                                                      gCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTTGCAGAAGCTGTCTGCCAAATATTTACCCTTTCCCAGGTCCACCAACGTCCGTTTAATTTACGTTAAAAAAGCTGACGCCCGCAGCTTTTATTAAAAAGAGACGCTCCGCtg                                                 >  1:642795/1‑150 (MQ=255)
                                                                                                       cAGACACCGTCTGCAGAATTCCCATCGGCAAAGAGTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgt                                                <  2:3487006/150‑1 (MQ=255)
                                                                                                        aGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGACTGCCAACTCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTGTCCTTTAAAAACTCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgtg                                               <  2:1559020/150‑1 (MQ=255)
                                                                                                         gACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                              <  1:2961823/150‑1 (MQ=255)
                                                                                                           cacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt                                            <  1:2386737/150‑1 (MQ=255)
                                                                                                              cGTCTGCAGAATTCCCATCGGCCAAGATTTGGCAGACGCTGTCTGCCAACTCGTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGt                                         <  2:2549592/150‑1 (MQ=255)
                                                                                                              cGTCTGCAGAATTCCCATCGGCAACGATTTGGCAGCCGCTGACTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTgga                                         <  1:1725337/150‑2 (MQ=255)
                                                                                                                     aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCg                                  <  1:3346979/150‑1 (MQ=255)
                                                                                                                        aTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCa                               <  1:882824/150‑1 (MQ=255)
                                                                                                                           cccATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCt                            <  2:438262/150‑1 (MQ=255)
                                                                                                                             cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                          >  2:227660/1‑150 (MQ=255)
                                                                                                                              aTCGGCAAAGATTTGGCAGACGCTGTCTGCCAACTCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTaga                         <  2:1829795/150‑1 (MQ=255)
                                                                                                                              aTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGGCGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTaga                         <  1:3472588/150‑1 (MQ=255)
                                                                                                                               tCGGCAAAGATGTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTagag                        <  2:3202669/150‑1 (MQ=255)
                                                                                                                                cGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGc                       <  1:3435956/150‑1 (MQ=255)
                                                                                                                                cGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGc                       <  1:2366437/150‑1 (MQ=255)
                                                                                                                                cGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGc                       <  2:1075073/150‑1 (MQ=255)
                                                                                                                                       gATTTGGCAGACAGCGTCTGCCAAATCTTTGCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAt                >  1:1010420/1‑150 (MQ=255)
                                                                                                                                         tttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg              >  2:2121439/1‑150 (MQ=255)
                                                                                                                                         tttGGCAGACAGCGTCTGCCAAATCTTTGCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg              >  2:3792084/1‑150 (MQ=255)
                                                                                                                                         tctGGCAGACAGCGTCTGCCAAATCTTTGCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg              >  2:1523439/3‑150 (MQ=255)
                                                                                                                                            ggCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCTAATTGCGa           <  2:3530655/150‑1 (MQ=255)
                                                                                                                                            ggCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTGTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCCGCTCGAGCGGCACATTGCGa           >  2:1060155/1‑150 (MQ=255)
                                                                                                                                             gCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAc          >  2:514015/1‑150 (MQ=255)
                                                                                                                                               aGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAccc        >  2:1453862/1‑150 (MQ=255)
                                                                                                                                                gACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCa       <  1:3616620/150‑1 (MQ=255)
                                                                                                                                                  cGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGt     >  1:2298736/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTC‑TTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTT  >  NC_000913/3368073‑3368370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑