New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4388615 =	79 (1.150)	3 (0.050)	3/262	NT	4.1%	coding (756/3324 nt)	mscM	mechanosensitive channel protein, miniconductance
	?	NC_000913	4388655 =	64 (0.990)					coding (716/3324 nt)	mscM	mechanosensitive channel protein, miniconductance
Rejected: Frequency below/above cutoff threshold.

CGTCAGCTTGAGGCGGAGCGGGCGCTGGAAAGTACCGAATTGCTGGCAGAAAACAGCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4388674‑4388615 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgccCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAGACAGCTGCGCCAGCTC  >  NC_000913/4388655‑4388800                                                                                                                                                                                                                CGTCAGCTTGAGGCGGAGCGGGCGCTGGAAAGTACCGAATTGCTGGCAGAAAACAGCG‑CCGATTTGCCGAAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGC                                                          >  2:718765/1‑149                    GGGCGCTGGAAAGTACCGAATTGCTGGCAGAAAACAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAATGCCAGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCAAGCTCTGAGCGTAAGCGCTCT                                        >  1:861399/1‑149                     GGCGCTGGAAAGTACCGAATTGCTGGCAGAAAACAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCAAGCTCTGAGCGTAAGCGCGCTA                                       >  2:715096/1‑149                                                     ctAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCGGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAGACAGCTGCGCC       >  1:840960/3‑149                                                          GCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAGACAGCTGCGCCAGCTC  <  2:947110/149‑1                                                                                                                                                                                                                CGTCAGCTTGAGGCGGAGCGGGCGCTGGAAAGTACCGAATTGCTGGCAGAAAACAGCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4388674‑4388615 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgccCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAGACAGCTGCGCCAGCTC  >  NC_000913/4388655‑4388800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.