| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,988,051 | A→T | 11.7% | intergenic (‑92/‑171) | azuC ← / → yecR | acid‑inducible small membrane‑associated protein/lipoprotein, function unknown |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,988,051 | 0 | A | T | 11.7% | 140.2 / 6.6 | 58 | intergenic (‑92/‑171) | azuC/yecR | acid‑inducible small membrane‑associated protein/lipoprotein, function unknown |
| Reads supporting (aligned to +/- strand): ref base A (28/23); new base T (2/5); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTT > NC_000913/1987903‑1988198 | tttGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTACGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGGGATACaa < 1:767006/149‑1 (MQ=255) tagAACGTCTTGCAATAGTTATTGAACATACTATTCAGGATTTAGCGCAGTTTCATAGCGTCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATAAATCTTTATGTGATACaaa < 2:546864/147‑1 (MQ=255) tGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAATATCAATCTTTATGTGATACAAAt > 2:248565/1‑149 (MQ=255) tGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAAt > 1:522650/1‑149 (MQ=255) tCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATa > 1:467082/1‑149 (MQ=255) cTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATaa > 1:1110347/1‑149 (MQ=255) cTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATaa > 1:483836/1‑149 (MQ=255) ttGGAATAGTTATTGAACATACTATTCAGGATTTTGCGCAGTTTCATCGCGGCACTGCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAACTCAATCTTTATGTGCTTTGTATCACATaaa < 1:750455/149‑1 (MQ=255) ttGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTTATCGCGGCACTCCGACGATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATTATATGACAACCATCACAAAAATCAATCTTTATGTGTTACAAATCACATaat < 2:277852/149‑2 (MQ=255) ttGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTACGACCATTTGTTATACAGGTTTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATTTGTATCACATaaa < 2:197948/149‑1 (MQ=255) cAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATTTGTATCACATaaagat < 1:337927/149‑4 (MQ=255) cAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATTTGTATCACATaaagat < 1:652340/149‑4 (MQ=255) cAATACTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTAAAATATGACAACCATCACAAAAATCAATCTTTATGTGATTTGTATCACATaaagat < 1:303742/149‑4 (MQ=255) aaTAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATAcc > 2:888514/1‑149 (MQ=255) tGAACATACTTTTCCGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAATTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATg < 1:972480/149‑1 (MQ=255) aaCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGtt < 2:41429/149‑1 (MQ=255) aaCATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGtt < 2:522650/149‑1 (MQ=255) aaCATACTTTTCAGGATTTTGAGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGtt < 2:9519/149‑1 (MQ=255) cATACTTGTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTtat > 1:81197/1‑149 (MQ=255) ttCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACATATCACATAAATACCCCTTTAATGTTATATAAAtt > 1:316896/1‑148 (MQ=255) cAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACAGAAATACCCCTTTAATGTTATAAAAATGAt > 2:696568/1‑149 (MQ=255) aGGATTTTGCGCAGTTTCATCTCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATa < 1:1183265/149‑1 (MQ=255) cgcAGTTTCATCGCGGCGCTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATAACATAAATACCCCATTAAGGTTATAAAAATGATAATCaaaaaa > 1:491575/1‑149 (MQ=255) tttCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGcc < 1:222276/149‑1 (MQ=255) cATCGCGTCACTCCGAGCAGTTGTTATACAGGTGTTATTGTCTTTGCGCTTAAAATATGACAACCATCACAAAACTCAATCATTATGTGATACAAATCAAATAAATACCCGTTTAATGTTATAAAAATGATAATCAAAAAACAGacccc > 2:1048166/1‑149 (MQ=255) tCGCGGCAATCCGAACATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGAGAACCATCAAAAAAATCAATCTTTATGTGTTACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCt < 2:562285/149‑1 (MQ=255) ggCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCAAttt < 2:390280/149‑1 (MQ=255) cTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGa < 1:508238/149‑1 (MQ=255) tCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGac < 2:172667/149‑1 (MQ=255) ccATTTGTTATACAGGTGTTATTGTCTTTGCTCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTa < 2:620512/149‑1 (MQ=255) tttGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAg > 1:580608/1‑149 (MQ=255) tttGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAg < 1:888514/149‑1 (MQ=255) tttGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAg > 2:937152/1‑149 (MQ=255) gTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATg > 2:151552/1‑149 (MQ=255) ttATACAGGTGATATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTAAGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATgg < 2:489888/149‑1 (MQ=255) aGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTTATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGaa < 1:922037/149‑1 (MQ=255) aGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGaa > 1:943221/1‑149 (MQ=255) gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAAt > 2:340440/1‑149 (MQ=255) gtgtTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAAt > 2:36298/1‑149 (MQ=255) tttttatGTCTTTGAGATTCTAATATCACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATa < 1:1092601/143‑1 (MQ=255) tttATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAg < 1:34737/148‑1 (MQ=255) ttATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATTATAATCAAAAAACAGCCACCCTATTTCTGACACCTACAGATGAAAAGAAATAgc > 1:819541/1‑149 (MQ=255) ttATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCACCAATTTCAGACACCTACAGATGGCAAGAAATTgc > 1:894924/1‑149 (MQ=255) tATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAgcg > 2:50382/1‑149 (MQ=255) gTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCATAAAACAGCCCCCCTATTACTGACACCTACAGAGGGCAAGAAATAGCGCCTg > 2:444000/1‑149 (MQ=255) cTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTc < 2:580608/149‑1 (MQ=255) ataataTGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATTTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGAGCCTGCCAGGAGTCttt > 1:909786/1‑149 (MQ=255) aataTGACAACCACCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTc < 1:296746/149‑1 (MQ=255) atatGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAAGGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTCACACCTACAGATGGCAATAAATAGCGCCTGCCAGGAGACTTTTcc > 2:1074995/1‑149 (MQ=255) tGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCTCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGc < 2:483836/149‑1 (MQ=255) ccATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCAAGGCGTCTTTTCCGGCCATTGt > 2:1152896/1‑149 (MQ=255) tCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAATACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCgc < 2:81197/149‑1 (MQ=255) acaAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGATATAAAAATGAAAATCAAAAAACAGCCCCCCTATTACAGACCCCTACAGATGGAAAGAAATAGCGCCTGCCAGGCGTCTTTACCGGCCATTGTCgcag > 1:909748/1‑149 (MQ=255) caatcTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTaa > 2:830902/1‑149 (MQ=255) ggtatttATGTGATTTGTATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAAc > 2:248315/5‑149 (MQ=255) tttATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGt < 2:1102700/149‑1 (MQ=255) gtgATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAAt > 1:537156/1‑149 (MQ=255) tgATACAAATCACATAAATACCCCTTTACTGTTATAAAAATGATAATCAAAAAACAGCCACACTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTGATCCGGCCATTGTCGCAGCACGGTAACGCGTAAAATa > 2:922431/1‑149 (MQ=255) tgATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATa > 2:408761/1‑149 (MQ=255) aTACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGt < 2:467082/149‑1 (MQ=255) aaaTCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCtt > 1:633348/1‑149 (MQ=255) | TTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCTTATAATATGACAACCATCACAAAAATCAATCTTTATGTGATACAAATCACATAAATACCCCTTTAATGTTATAAAAATGATAATCAAAAAACAGCCCCCCTATTTCTGACACCTACAGATGGCAAGAAATAGCGCCTGCCAGGCGTCTTTTCCGGCCATTGTCGCAGCACTGTAACGCGTAAAATAGTGCTT > NC_000913/1987903‑1988198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |