Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,592,989 A→G 7.1% intergenic (+27/+83) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,592,9890AG 7.1% 249.2 / 7.2 84intergenic (+27/+83)ypfN/ypfHputative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base A (39/39);  new base G (2/4);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAA  >  NC_000913/2592845‑2593135
                                                                                                                                                |                                                                                                                                                  
gcgcGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGc                                                                                                                                                <  2:909292/149‑1 (MQ=255)
gcgcGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGc                                                                                                                                                <  1:185831/149‑1 (MQ=255)
  gcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGCCGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGt                                                                                                                                              >  1:131388/1‑149 (MQ=255)
         ccATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGCCGCCGACTGGCCGAAAAAGGATCACACGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgcc                                                                                                                                       >  2:999000/1‑149 (MQ=255)
         ccATAAGAAATTTCTCGCCAACAAGCCGGAGCGTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCATCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgcc                                                                                                                                       <  1:44196/149‑1 (MQ=255)
          cATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgccg                                                                                                                                      <  2:459749/149‑1 (MQ=255)
          cATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgccg                                                                                                                                      >  1:565133/1‑149 (MQ=255)
              aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc                                                                                                                                  >  2:267153/1‑149 (MQ=255)
               gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc                                                                                                                                 >  2:101918/1‑149 (MQ=255)
               gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCGCCACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc                                                                                                                                 >  1:876514/1‑149 (MQ=255)
                taaTTTCTCGCGAAGATGCCGGCGCTTCCCCCGCATCGTGATTTCAAGGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCGCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                <  2:250393/148‑1 (MQ=255)
                aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                <  1:1020306/149‑1 (MQ=255)
                aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                <  1:722534/149‑1 (MQ=255)
                aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                <  1:427793/149‑1 (MQ=255)
                 aaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                                                               <  1:162974/149‑1 (MQ=255)
                     tctcGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                           >  2:290592/1‑149 (MQ=255)
                         gCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTg                                                                                                                       >  1:36250/1‑149 (MQ=255)
                           caacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGaa                                                                                                                     <  1:975988/149‑1 (MQ=255)
                            aacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGCCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                                                                                                    <  2:930144/149‑1 (MQ=255)
                            aacaaGCCGGAGCTTCCCACGCATCGTGATATCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                                                                                                    <  2:958983/149‑1 (MQ=255)
                             acaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACg                                                                                                                   >  2:868850/1‑149 (MQ=255)
                                  ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGcc                                                                                                              <  2:672868/149‑1 (MQ=255)
                                   cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt                                                                                                             >  1:1081007/1‑149 (MQ=255)
                                    ggAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTg                                                                                                            <  1:748523/149‑1 (MQ=255)
                                          tCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCgc                                                                                                      >  1:292134/1‑148 (MQ=255)
                                           cccccGCATCGTGATTTCAACGATTAGTGGGACGATGACGACGACTGGCCGAATATTTATCAACCGAAGAAGTAAGCTGAGTGCTCAAGCGTAGGCCGGATAAGTGGTTAACGCGTCATCCGGCAATGGTGATGGATGCCTGATGCGAc                                                                                                     <  1:147842/149‑1 (MQ=255)
                                              ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGAAATGGTGAACGATGCCTGATGCGACGCt                                                                                                  >  2:387791/1‑149 (MQ=255)
                                                  ctCGTGATCTCAACGATAAGTGGGACGATGCCGACCACTCGCCGAAAAAGGATCAACCGCAGACGTAAGCAGAGTGCTCAACCGTGGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg                                                                                              <  1:818348/148‑1 (MQ=37)
                                                        aTTTCAACGATAAGTGGGACGATGACGCCGAGTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGTCCGGATAAGGCGTTAACGCCGCATCCTGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCtt                                                                                        <  1:881735/149‑1 (MQ=255)
                                                          ttCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt                                                                                      >  1:287589/1‑149 (MQ=255)
                                                           tCAACGATAAGTGGGCCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                     >  1:15416/1‑149 (MQ=255)
                                                           tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                     <  2:455385/149‑1 (MQ=255)
                                                           tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                     <  2:540608/149‑1 (MQ=255)
                                                           tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                     <  2:866541/149‑1 (MQ=255)
                                                                 aTAAGTGCGACGATGACGACGACTGGCCGAAAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCa                                                                               <  1:733778/149‑1 (MQ=255)
                                                                  ttaGTGGTACGATGAGGACGACTTGCCGATAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAAACGGAGGCCGGATAAGGCTTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGAGACGCTCACGCGTCTTATCAgtcaag                                                                              <  2:264485/147‑6 (MQ=37)
                                                                  tAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTACGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAg                                                                              <  2:620018/149‑1 (MQ=255)
                                                                       gtgACGATGACGACGCCTGGCCGAAAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTATGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCGCACGCGTCTTATCAGGCCAGGTAAt                                                                         <  2:1039148/147‑1 (MQ=255)
                                                                       gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt                                                                         <  1:101918/149‑1 (MQ=255)
                                                                        ggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc                                                                        <  1:267153/149‑1 (MQ=255)
                                                                            gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTc                                                                    >  1:225077/1‑149 (MQ=255)
                                                                             ctGACGACGACTGGCCGAAACAGGATCAACCGAAGAAGTCAGCTGAGTGCTCAACCGTAGGCCGGAAAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCATGATGCGACTCTCACGCGTCTTATCAGGCCAGGGAATCTGTCt                                                                   <  1:755823/148‑1 (MQ=38)
                                                                               tacgaccacTGGCCGTATTAGGTTCAAGCGATGAAGTAAGCTGAGTGCTCAACAGTAGGCCGGATATGGCGTTAACGCCGCAGCCGGCAATGGTGATCGATGCCTGCTGGGACGCTGACGCGTCTTATCAGGCCAGGTATGCTGTCttt                                                                 <  1:231848/148‑1 (MQ=25)
                                                                               gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                 <  1:273385/149‑1 (MQ=255)
                                                                               gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                 >  1:901211/1‑149 (MQ=255)
                                                                               gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                 >  1:983041/1‑149 (MQ=255)
                                                                                acgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                <  2:1001535/149‑1 (MQ=255)
                                                                                acgacgacTGGCCGAAAAAGGATAAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                <  1:360444/149‑1 (MQ=255)
                                                                                  gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAc                                                              >  1:332156/1‑149 (MQ=255)
                                                                                   acgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAca                                                             >  1:179752/1‑149 (MQ=255)
                                                                                    cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcat                                                            <  2:131388/149‑1 (MQ=255)
                                                                                     gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCGGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcaaa                                                           >  2:1026283/1‑147 (MQ=255)
                                                                                     gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcatc                                                           >  1:242666/1‑149 (MQ=255)
                                                                                             gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGGGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCCGGTATTCTGTCTTTTACATCATTTCAAt                                                   >  2:806506/1‑149 (MQ=255)
                                                                                              aaaaaGGATCAAGCGAAGAAGTAAGCTGATTGCTCAACCGTAGGCCGGATAAGGAGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATc                                                  <  1:569104/149‑1 (MQ=255)
                                                                                               aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATca                                                 >  2:188084/1‑149 (MQ=255)
                                                                                                aaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcac                                                <  1:246404/149‑1 (MQ=255)
                                                                                                    gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatca                                            >  1:274173/1‑149 (MQ=255)
                                                                                                      tCACCCGACTAAGGAAGCTGAGGGCACCACCGGAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                          <  2:664265/149‑1 (MQ=25)
                                                                                                      tCAACCGAAGAAGTAAGCTGAGTGCTCAACCTTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                          >  2:246888/1‑149 (MQ=255)
                                                                                                      tCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCTTTTCAATCAcatcatc                                          <  1:619711/149‑1 (MQ=255)
                                                                                                       cAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatca                                         <  1:867141/149‑1 (MQ=255)
                                                                                                       cAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatca                                         >  2:1174873/1‑149 (MQ=255)
                                                                                                        aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatcat                                        >  2:438099/1‑149 (MQ=255)
                                                                                                          ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCg                                      <  2:174560/149‑1 (MQ=255)
                                                                                                            gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGcc                                    <  2:233039/149‑1 (MQ=255)
                                                                                                               taagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAgg                                 <  2:472364/148‑1 (MQ=255)
                                                                                                                   tAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCCTCGCCAGGCTTa                             >  2:974302/1‑149 (MQ=255)
                                                                                                                         gAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCACCATCGCCAGGCTTACCGCCa                       >  2:180247/1‑149 (MQ=255)
                                                                                                                           gTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACt                     <  2:36250/149‑1 (MQ=255)
                                                                                                                              cTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATATCAATCACATCCTCATCGCCAGGCTTACCGCCACTTAg                  >  2:564899/1‑149 (MQ=255)
                                                                                                                               tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                 >  1:508811/1‑149 (MQ=255)
                                                                                                                               tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                 >  1:754742/1‑149 (MQ=255)
                                                                                                                                cAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcg                >  2:1142003/1‑149 (MQ=255)
                                                                                                                                 aaCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcgc               >  1:217070/1‑149 (MQ=255)
                                                                                                                                  aCCGTAGGCCGGATAAGGCTTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCt              <  1:793643/149‑1 (MQ=255)
                                                                                                                                   ccGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCCAAGGTGAACGCTGCCTGATGCGACGCTCACGCCTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAAATCATCATCTCCAGGCTTACCGCCACTTTGAGCtt             >  1:640427/1‑149 (MQ=37)
                                                                                                                                    cGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCCCATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtc            >  1:469614/1‑149 (MQ=255)
                                                                                                                                    cGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCCGGCTTCCCGCCCCGTAGCGCttg            >  2:90369/1‑148 (MQ=255)
                                                                                                                                         gCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGa       >  1:1061403/1‑149 (MQ=255)
                                                                                                                                          ccGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaa      >  1:945501/1‑149 (MQ=255)
                                                                                                                                           cGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaaa     >  1:559423/1‑149 (MQ=255)
                                                                                                                                           cGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTGTTTTACATCATATCAATCACATAATCATCGCCAGGCTTACCGCAACTTAGCTCTTCCTCGaaa     >  2:119251/1‑149 (MQ=37)
                                                                                                                                              aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaataa  <  1:552768/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAA  >  NC_000913/2592845‑2593135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: