Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,673,784 C→A 9.5% intergenic (+16/+32) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7840CA 9.5% 152.3 / 4.7 69intergenic (+16/+32)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
Reads supporting (aligned to +/- strand):  ref base C (35/27);  new base A (4/3);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTC  >  NC_000913/2673640‑2673932
                                                                                                                                                |                                                                                                                                                    
tACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcggcg                                                                                                                                                  >  1:206240/1‑149 (MQ=255)
 aCTGGGATATGACCGGCGATGAGGTTGGGCCAAATATGATTAATATCTGGAAGAATGTCCGTATCGCTGGCGCGTTCTTGCTATTGTCAATTACCGGGCCGGGTGCAATTTATCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGt                                                                                                                                                 <  1:860058/149‑1 (MQ=255)
   tGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGa                                                                                                                                               >  1:214573/1‑149 (MQ=255)
       atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGc                                                                                                                                           >  1:336425/1‑149 (MQ=255)
        tatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGcc                                                                                                                                          <  1:584735/149‑1 (MQ=255)
        tatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGcc                                                                                                                                          <  1:731950/149‑1 (MQ=255)
          tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCtt                                                                                                                                        <  1:625612/149‑1 (MQ=255)
           gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                       >  1:1006669/1‑149 (MQ=255)
                    tGCGGTTGGGCCAAATCTGATTAATTTCTGGAAGAATGTCTGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGTGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCAtc                                                                                                                              <  2:414539/149‑2 (MQ=38)
                       ggTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTTCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                                                                           <  1:912094/149‑1 (MQ=255)
                        gTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTTCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                                                          >  2:770350/1‑149 (MQ=255)
                             gCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAtt                                                                                                                     >  1:352567/1‑149 (MQ=255)
                               cAACTATGATTAATTTCTGGAAGAATGTCAGTATCGCCGGCGCGTTCGTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCTTTAGGATAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTAc                                                                                                                   <  1:761363/149‑1 (MQ=255)
                                aaaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGTATTTAATAAATTACa                                                                                                                  >  1:891409/1‑149 (MQ=255)
                                  atatGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                                <  1:589393/149‑1 (MQ=255)
                                      gATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCAct                                                                                                            >  2:287687/1‑149 (MQ=255)
                                       aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActc                                                                                                           >  2:851006/1‑149 (MQ=255)
                                        ttAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActct                                                                                                          >  2:13810/1‑149 (MQ=255)
                                           aTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                       >  2:460306/1‑149 (MQ=255)
                                           aTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                       >  1:1079493/1‑149 (MQ=255)
                                           aTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTAGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTAAAGCCACTCTAcc                                                                                                       >  1:1021694/1‑149 (MQ=255)
                                                  gaagaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACg                                                                                                >  1:1106597/1‑149 (MQ=255)
                                                    agaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGAc                                                                                              <  2:336425/149‑1 (MQ=255)
                                                        tGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCCGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATATAATAAATTACAGCCACTCTACCCGCAACGACAtca                                                                                          >  2:1129155/1‑148 (MQ=255)
                                                          tCAGGATCGCTGGCGCGTTCTTGCTATTTGCTATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATctct                                                                                        <  2:28660/149‑1 (MQ=255)
                                                            aGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGa                                                                                      >  1:115007/1‑149 (MQ=255)
                                                             gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGaa                                                                                     <  2:1067419/149‑1 (MQ=255)
                                                                  gCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATTCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAggtgt                                                                                >  2:544536/1‑149 (MQ=255)
                                                                       cgcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTCCCCGCACCGCCATCTCTGAAGTTGTTGAtt                                                                           >  2:773634/1‑149 (MQ=255)
                                                                        gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTc                                                                          >  2:857683/1‑149 (MQ=255)
                                                                        gcgTGCTTGCTATTGGCAATTACCGGGCCGGGGGCAAGTTCTCTCGATCGGCGTTAGGAAAGTCGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTc                                                                          <  1:541369/149‑1 (MQ=255)
                                                                              ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACTTCGCTGAAGTTGTTGATTCCCCTgg                                                                    >  1:329210/1‑149 (MQ=255)
                                                                              ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGGGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTgg                                                                    <  1:113790/149‑1 (MQ=255)
                                                                                  tATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGca                                                                >  2:390401/1‑148 (MQ=255)
                                                                                   aTTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTa                                                               <  1:785083/149‑1 (MQ=255)
                                                                                   aTTGGCAATTAACGGGCCGGGTGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTa                                                               <  2:9939/149‑1 (MQ=255)
                                                                                    ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAATATGACGGATGCCGCGTGAACGCCTTATCCGGCATATTATAAATTACAGACACTCTACACGAAACGCAATCTCTGAAGTTGTTTATTCCCATGGTGCTAg                                                              >  1:105727/1‑149 (MQ=255)
                                                                                    ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCCACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                              >  1:749880/1‑149 (MQ=255)
                                                                                    ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                              >  1:807983/1‑149 (MQ=255)
                                                                                       gCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGCACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc                                                           >  2:76685/1‑149 (MQ=255)
                                                                                         aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACTCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAGGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTTGCGCaa                                                         >  2:525856/1‑149 (MQ=255)
                                                                                         aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                         >  2:560424/1‑149 (MQ=255)
                                                                                          aTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt                                                        <  1:671893/149‑1 (MQ=255)
                                                                                           ttACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATg                                                       <  2:1079493/149‑1 (MQ=255)
                                                                                            tACCGGGCAGGGGGCAATTGCTCTCGATCGGCGGTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAACTTACAGCCACTCTACCCTCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATga                                                      <  2:912666/149‑1 (MQ=255)
                                                                                               cgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGAt                                                   >  1:407321/1‑149 (MQ=255)
                                                                                                     gggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAcccc                                             >  2:431727/1‑149 (MQ=255)
                                                                                                      ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAccccc                                            >  2:321765/1‑149 (MQ=255)
                                                                                                         gCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTc                                         <  1:460306/149‑1 (MQ=255)
                                                                                                           aaTTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCGTATCCGGCATTTAATAAATTAAAGCAACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTACCCTGGTGCTAGCGCAATGAGATCAcccaattagg                                       >  2:547090/1‑142 (MQ=255)
                                                                                                                 tctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                 <  1:857683/149‑1 (MQ=255)
                                                                                                                    cGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGTATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgat                              <  2:891409/149‑1 (MQ=255)
                                                                                                                     gATCGGCGTTATGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGAAACGACATCTCTGACGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                             <  1:770350/149‑1 (MQ=255)
                                                                                                                     gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                             >  2:1010602/1‑149 (MQ=255)
                                                                                                                      aTCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCGCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                            <  1:797052/149‑1 (MQ=255)
                                                                                                                         ggCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGatca                         <  2:105285/149‑1 (MQ=255)
                                                                                                                           cGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCGTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGCCATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGatcatc                       >  2:149845/1‑149 (MQ=255)
                                                                                                                             ttAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCg                     <  1:653922/149‑1 (MQ=255)
                                                                                                                               aGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgc                   <  2:214573/149‑1 (MQ=255)
                                                                                                                                ggAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcg                  >  2:233469/1‑149 (MQ=255)
                                                                                                                                 attaaaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCGCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                 >  1:1083705/6‑149 (MQ=38)
                                                                                                                                 attaaaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                 >  1:670839/6‑149 (MQ=38)
                                                                                                                                   aaGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCAt               <  1:1172035/149‑1 (MQ=255)
                                                                                                                                    aaaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATAATCCGGCGCATg              >  1:1092687/3‑149 (MQ=38)
                                                                                                                                     gATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATgg             <  1:996022/149‑1 (MQ=255)
                                                                                                                                     gATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATgg             <  2:456760/149‑1 (MQ=255)
                                                                                                                                         ccGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCa         >  2:153167/1‑149 (MQ=255)
                                                                                                                                          cGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCACCGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTcct        >  2:723659/1‑147 (MQ=255)
                                                                                                                                          cGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCag        >  1:388600/1‑148 (MQ=255)
                                                                                                                                              tGCGGCGGGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCCACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCGAGCGCAATGAGCTCACCCCCTTCCGGGCGATGAGGATCATCCGGCGCATGGCTCATCGGt    <  2:1153362/149‑1 (MQ=255)
                                                                                                                                               gcggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGtt   >  1:176692/1‑149 (MQ=255)
                                                                                                                                                cggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTc  <  2:352567/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTC  >  NC_000913/2673640‑2673932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑