Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,673,790 G→T 9.1% intergenic (+22/+26) yphA → / ← yphB DoxX family inner membrane protein/mutarotase superfamily protein, YphB family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7900GT 9.1% 174.2 / 8.8 73intergenic (+22/+26)yphA/yphBDoxX family inner membrane protein/mutarotase superfamily protein, YphB family
Reads supporting (aligned to +/- strand):  ref base G (38/28);  new base T (4/3);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

TGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGAC  >  NC_000913/2673643‑2673937
                                                                                                                                                   |                                                                                                                                                   
tGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGa                                                                                                                                                    >  1:214573/1‑149 (MQ=255)
    atatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGc                                                                                                                                                >  1:336425/1‑149 (MQ=255)
     tatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGcc                                                                                                                                               <  1:584735/149‑1 (MQ=255)
     tatGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGcc                                                                                                                                               <  1:731950/149‑1 (MQ=255)
       tGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCtt                                                                                                                                             <  1:625612/149‑1 (MQ=255)
        gACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                            >  1:1006669/1‑149 (MQ=255)
                 tGCGGTTGGGCCAAATCTGATTAATTTCTGGAAGAATGTCTGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGTGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATAAGGCGTTCACGCCGCATCCGGCAtc                                                                                                                                   <  2:414539/149‑2 (MQ=38)
                    ggTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTTCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaa                                                                                                                                <  1:912094/149‑1 (MQ=255)
                     gTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTTCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTtaat                                                                                                                               >  2:770350/1‑149 (MQ=255)
                          gCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAAtt                                                                                                                          >  1:352567/1‑149 (MQ=255)
                            cAACTATGATTAATTTCTGGAAGAATGTCAGTATCGCCGGCGCGTTCGTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCTTTAGGATAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTAc                                                                                                                        <  1:761363/149‑1 (MQ=255)
                             aaaTATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGTATTTAATAAATTACa                                                                                                                       >  1:891409/1‑149 (MQ=255)
                               atatGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGc                                                                                                                     <  1:589393/149‑1 (MQ=255)
                                   gATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCAct                                                                                                                 >  2:287687/1‑149 (MQ=255)
                                    aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActc                                                                                                                >  2:851006/1‑149 (MQ=255)
                                     ttAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCActct                                                                                                               >  2:13810/1‑149 (MQ=255)
                                        aTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                            >  2:460306/1‑149 (MQ=255)
                                        aTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTAcc                                                                                                            >  1:1079493/1‑149 (MQ=255)
                                        aTTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTAGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTAAAGCCACTCTAcc                                                                                                            >  1:1021694/1‑149 (MQ=255)
                                               gaagaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACg                                                                                                     >  1:1106597/1‑149 (MQ=255)
                                                 agaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGAc                                                                                                   <  2:336425/149‑1 (MQ=255)
                                                     tGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCCGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATATAATAAATTACAGCCACTCTACCCGCAACGACAtca                                                                                               >  2:1129155/1‑148 (MQ=255)
                                                       tCAGGATCGCTGGCGCGTTCTTGCTATTTGCTATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATctct                                                                                             <  2:28660/149‑1 (MQ=255)
                                                         aGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGa                                                                                           >  1:115007/1‑149 (MQ=255)
                                                          gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGaa                                                                                          <  2:1067419/149‑1 (MQ=255)
                                                               gCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATTCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAggtgt                                                                                     >  2:544536/1‑149 (MQ=255)
                                                                    cgcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTCCCCGCACCGCCATCTCTGAAGTTGTTGAtt                                                                                >  2:773634/1‑149 (MQ=255)
                                                                     gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTc                                                                               >  2:857683/1‑149 (MQ=255)
                                                                     gcgTGCTTGCTATTGGCAATTACCGGGCCGGGGGCAAGTTCTCTCGATCGGCGTTAGGAAAGTCGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTc                                                                               <  1:541369/149‑1 (MQ=255)
                                                                           ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACTTCGCTGAAGTTGTTGATTCCCCTgg                                                                         >  1:329210/1‑149 (MQ=255)
                                                                           ttGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGGGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTgg                                                                         <  1:113790/149‑1 (MQ=255)
                                                                               tATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGca                                                                     >  2:390401/1‑148 (MQ=255)
                                                                                aTTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTa                                                                    <  1:785083/149‑1 (MQ=255)
                                                                                aTTGGCAATTAACGGGCCGGGTGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTa                                                                    <  2:9939/149‑1 (MQ=255)
                                                                                 ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAATATGACGGATGCCGCGTGAACGCCTTATCCGGCATATTATAAATTACAGACACTCTACACGAAACGCAATCTCTGAAGTTGTTTATTCCCATGGTGCTAg                                                                   >  1:105727/1‑149 (MQ=255)
                                                                                 ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCCACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                   >  1:749880/1‑149 (MQ=255)
                                                                                 ttGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAg                                                                   >  1:807983/1‑149 (MQ=255)
                                                                                    gCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGCACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc                                                                >  2:76685/1‑149 (MQ=255)
                                                                                      aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACTCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAGGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTTGCGCaa                                                              >  2:525856/1‑149 (MQ=255)
                                                                                      aaTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCaa                                                              >  2:560424/1‑149 (MQ=255)
                                                                                       aTTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt                                                             <  1:671893/149‑1 (MQ=255)
                                                                                        ttACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATg                                                            <  2:1079493/149‑1 (MQ=255)
                                                                                         tACCGGGCAGGGGGCAATTGCTCTCGATCGGCGGTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAACTTACAGCCACTCTACCCTCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATga                                                           <  2:912666/149‑1 (MQ=255)
                                                                                            cgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGAt                                                        >  1:407321/1‑149 (MQ=255)
                                                                                                  gggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAcccc                                                  >  2:431727/1‑149 (MQ=255)
                                                                                                   ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCAccccc                                                 >  2:321765/1‑149 (MQ=255)
                                                                                                      gCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTc                                              <  1:460306/149‑1 (MQ=255)
                                                                                                        aaTTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCGTATCCGGCATTTAATAAATTAAAGCAACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTACCCTGGTGCTAGCGCAATGAGATCAcccaattagg                                            >  2:547090/1‑142 (MQ=255)
                                                                                                              tctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgat                                      <  1:857683/149‑1 (MQ=255)
                                                                                                                 cGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGTATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgat                                   <  2:891409/149‑1 (MQ=255)
                                                                                                                  gATCGGCGTTATGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGAAACGACATCTCTGACGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                                  <  1:770350/149‑1 (MQ=255)
                                                                                                                  gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg                                  >  2:1010602/1‑149 (MQ=255)
                                                                                                                   aTCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCGCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga                                 <  1:797052/149‑1 (MQ=255)
                                                                                                                      ggCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGatca                              <  2:105285/149‑1 (MQ=255)
                                                                                                                        cGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCGTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGCCATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGatcatc                            >  2:149845/1‑149 (MQ=255)
                                                                                                                          ttAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCg                          <  1:653922/149‑1 (MQ=255)
                                                                                                                            aGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgc                        <  2:214573/149‑1 (MQ=255)
                                                                                                                             ggAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcg                       >  2:233469/1‑149 (MQ=255)
                                                                                                                              attaaaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCGCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                      >  1:1083705/6‑149 (MQ=38)
                                                                                                                              attaaaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc                      >  1:670839/6‑149 (MQ=38)
                                                                                                                                aaGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCAt                    <  1:1172035/149‑1 (MQ=255)
                                                                                                                                 aaaTGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATAATCCGGCGCATg                   >  1:1092687/3‑149 (MQ=38)
                                                                                                                                  gATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATgg                  <  2:456760/149‑1 (MQ=255)
                                                                                                                                  gATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATgg                  <  1:996022/149‑1 (MQ=255)
                                                                                                                                      ccGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCa              >  2:153167/1‑149 (MQ=255)
                                                                                                                                       cGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCACCGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTcct             >  2:723659/1‑147 (MQ=255)
                                                                                                                                       cGGATAAGGCGTTCACGCCGCATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCag             >  1:388600/1‑148 (MQ=255)
                                                                                                                                           tGCGGCGGGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCCACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCGAGCGCAATGAGCTCACCCCCTTCCGGGCGATGAGGATCATCCGGCGCATGGCTCATCGGt         <  2:1153362/149‑1 (MQ=255)
                                                                                                                                            gcggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGtt        >  1:176692/1‑149 (MQ=255)
                                                                                                                                             cggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTc       <  2:352567/149‑1 (MQ=255)
                                                                                                                                              ggcgTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGGTcc      >  1:759329/1‑149 (MQ=255)
                                                                                                                                                 gTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcaga   <  1:250791/149‑1 (MQ=255)
                                                                                                                                                  tGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACCTCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTGCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcagac  >  2:445820/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGAC  >  NC_000913/2673643‑2673937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑