Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,775,505 G→A 5.8% intergenic (+484/‑40) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,775,5050GA 5.8% 276.0 / 6.0 105intergenic (+484/‑40)yfjW/ypjICP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (44/53);  new base A (2/4);  total (47/58)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 107 total aligned reads displayed.

TAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAT  >  NC_000913/2775357‑2775648
                                                                                                                                                    |                                                                                                                                               
tAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTATGTCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg                                                                                                                                                 >  2:197313/1‑149 (MQ=255)
 aaaTGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTCGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAgg                                                                                                                                                <  1:609917/149‑1 (MQ=255)
     gCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTc                                                                                                                                            >  1:108222/1‑149 (MQ=255)
      ccccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTcg                                                                                                                                           >  2:1161266/1‑148 (MQ=255)
      cacatACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCAATTCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCt                                                                                                                                           <  2:1060970/145‑1 (MQ=255)
        ccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCTGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCATGATTCTGc                                                                                                                                         >  1:1045621/1‑149 (MQ=255)
        ccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGc                                                                                                                                         <  1:524538/149‑1 (MQ=255)
         cTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGcc                                                                                                                                        <  2:870780/149‑1 (MQ=255)
         cTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGcc                                                                                                                                        <  1:203350/149‑1 (MQ=255)
           aCAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGAATAACGGCAGAATCTCAGGATTCTGCCGc                                                                                                                                      >  2:614512/1‑149 (MQ=255)
            cAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCt                                                                                                                                     >  2:245238/1‑149 (MQ=255)
             aaaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCgtta                                                                                                                                    <  2:99073/149‑4 (MQ=255)
              aaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttt                                                                                                                                   <  1:616008/149‑1 (MQ=255)
               aaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttt                                                                                                                                  >  2:57338/1‑149 (MQ=255)
                aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTGCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttttt                                                                                                                                 <  2:666205/149‑1 (MQ=255)
                aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttttt                                                                                                                                 >  2:302473/1‑149 (MQ=255)
                aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttttt                                                                                                                                 <  2:385122/149‑1 (MQ=255)
                   aaCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttat                                                                                                                              >  2:171128/1‑149 (MQ=255)
                    aCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttatt                                                                                                                             <  2:897258/149‑1 (MQ=255)
                       attattTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTattatt                                                                                                                          >  2:30864/1‑149 (MQ=255)
                        ttattTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTAttt                                                                                                                         >  2:104515/1‑149 (MQ=255)
                             tttAGATGTCGGCTGGAACTCCATCTTCCTCTTTTGGCACAGAACTGCCTTTCATCAATATAAAAGCATTCAGATATATATGATTTCTACAAACATACCCGATTAACGGCAGCATCGCAGGATTCTGCCGCTTTTTTATTATTTTTCAg                                                                                                                    <  1:1075053/149‑1 (MQ=255)
                              ttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAgg                                                                                                                   <  2:154673/149‑1 (MQ=255)
                              ttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAgg                                                                                                                   <  2:52587/149‑1 (MQ=255)
                               tAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAggg                                                                                                                  >  1:447564/1‑149 (MQ=255)
                               tAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAggg                                                                                                                  <  2:354470/149‑1 (MQ=255)
                                 gagGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGa                                                                                                                <  2:353674/149‑1 (MQ=255)
                                  agGGCGTCTGGCAATCCAACTTCCGCTTTTTGCACAGAACGGCCTATCAGCAATATATAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGag                                                                                                               <  1:660047/149‑1 (MQ=255)
                                       gTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAgtagta                                                                                                          >  2:15622/1‑149 (MQ=255)
                                       gTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAgtagta                                                                                                          >  2:350159/1‑149 (MQ=255)
                                          tGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGGATTCAGATATATATCATTTCTACAAACATCCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATg                                                                                                       <  1:675374/149‑1 (MQ=255)
                                          tGGCACTCCAACTTCCGCTTTTGGCAAATAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGTAGTAGTAATg                                                                                                       <  2:991724/149‑1 (MQ=255)
                                          tGGCACTCCAACTTCCGCTTTTCGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCGTTTTTATTATTTTTCAGGGGAGTAGTAATg                                                                                                       >  1:768173/1‑149 (MQ=255)
                                           ggCACTCCAACATCCGCTTTAGGCACAGAACGGCCTAGCAGCAAGATAAAAGCATTCAGATATATATCATTACTACAAACATACCCGATTAACGGCAGAAACTCAGCATTATGCCGCTTTTTTATTTTTTTTCAGGGGAGTAGAAATgc                                                                                                      >  2:816371/1‑148 (MQ=255)
                                            gCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAg                                                                                                     >  2:721983/1‑149 (MQ=255)
                                            gCACTCAAACTTCCGCTTTTGGCACATAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAg                                                                                                     <  2:495437/149‑1 (MQ=255)
                                               cTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCaa                                                                                                  <  2:958605/149‑1 (MQ=255)
                                                 ccAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAAct                                                                                                <  2:43362/149‑1 (MQ=255)
                                                  cAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAActc                                                                                               >  1:1115318/1‑149 (MQ=255)
                                                   aaCTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATAAACGGCAGAATCTCAGGATTCTGCCGATTTTTAATAAATTTTCAGGGGAGTAGTAATGAGCAActct                                                                                              >  2:1158988/1‑149 (MQ=255)
                                                      ttCCGCTTTTGGCACAGACAGGCCTATCAGCAAAATAAAAGCATTCAGATATATATCATTTCTACACACATACCCGATAAACGGCAGAATCTCAGGATTCTTCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATTAGCAACTCTGaa                                                                                           <  2:1003551/149‑1 (MQ=255)
                                                       tCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAg                                                                                          >  1:159425/1‑149 (MQ=255)
                                                       tCCGCTTTTGGCAAAGAACGGCCTATCATCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAg                                                                                          <  1:526702/149‑1 (MQ=255)
                                                         cGCTTTTGGCAGAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGt                                                                                        <  2:862869/149‑1 (MQ=255)
                                                         cGCTTTTGGCACAGAACGGCCGATCAGCAATATAAAAGCATTCAGATATATATCCTTTCTACAAACATACCCGATTAACGGCAGAATCTCAGTATTCTGCCGGTTTTTTATTATTTTTAAGGGGAGTAGTAATGAGCAACTCTGAAGGt                                                                                        >  1:744236/1‑149 (MQ=255)
                                                           cTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTg                                                                                      <  2:560886/149‑1 (MQ=255)
                                                              ttGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTa                                                                                   <  1:291717/149‑1 (MQ=255)
                                                                  cacaGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCa                                                                               >  1:1083426/1‑149 (MQ=255)
                                                                    caGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtt                                                                             >  1:260902/1‑149 (MQ=255)
                                                                     aGAACGGCCTATCAGCAATATAAAAGCATTCAGTTATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAttt                                                                            <  1:322255/149‑1 (MQ=255)
                                                                     aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTTTTATTTTTCAGGGGAGTAGTAATGAGAAACTCTGAAGGTTTGTAGTCAttt                                                                            >  1:1181802/1‑149 (MQ=255)
                                                                       aaCGGCCTATCAGCAATATAAAAGCATTCTGATATATAGCATTTCTACAAACATACCCGATTAACGGCAGAAGCTCCGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                          <  1:802364/149‑1 (MQ=255)
                                                                       aaCGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                          >  2:77139/1‑149 (MQ=255)
                                                                        aCGGCCTATCAGCTATATAAAAGCATTCAGATATATTTCATTTTTACTAACACACCGGATTAACGGCATAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCATGGGAGTAGTAATGAGCAACTCTGAAGGTTGTTAGTCATTTTAg                                                                         <  2:493855/149‑1 (MQ=255)
                                                                         cGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTATGCCGCTTTTTTATTATTTTTAAGGGGAGTAGTAATGATCAACTCTGAATGTTTGTAGTCATTTTata                                                                        >  1:311965/1‑147 (MQ=255)
                                                                                tCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTTAAGGTTGGTAGTCATTTTAGCAAACGCt                                                                 >  2:493085/1‑149 (MQ=255)
                                                                                tCAGCAATATAAAAGCATTAAGATATATATCATTTCTACAAACATACCCGATTATCTTCAGAATCTCAGGATTCTGCCTCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCt                                                                 <  2:859818/149‑1 (MQ=255)
                                                                                     aaTATTAAAGCATTCAGTTATATATCATTTCTACATACATTCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  1:370321/149‑1 (MQ=255)
                                                                                     aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTTTTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  1:350159/149‑1 (MQ=255)
                                                                                     aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  2:163827/149‑1 (MQ=255)
                                                                                     aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCGGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  2:108222/149‑1 (MQ=255)
                                                                                     aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATGCTGCCGCTTTTTTATTATTTATCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  1:1032026/149‑1 (MQ=255)
                                                                                     aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGAGTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGTTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  1:996669/149‑1 (MQ=255)
                                                                                          aaaaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTg                                                       >  1:619083/1‑149 (MQ=255)
                                                                                            aaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGGTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGcc                                                     <  1:972167/149‑1 (MQ=255)
                                                                                                aTTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAg                                                 >  2:583744/1‑149 (MQ=255)
                                                                                                  tCAGATATATATAATATCTACACCCCTCCCGTATTAACGGCACACTCTCTGGATTCGGCCGCTTTCTTATTATTTTTCAGCCGAGTAGTAATGAGCAACTCTGAAGGGTGGTAGTCATTTTCGCAAACGCTTTCTGGTCTGCCGCAGTg                                               <  2:302005/149‑1 (MQ=255)
                                                                                                   ctgATATATATCATTTCTACAAACATACCCGATTAACGGCCGAATCTCAGGATTCTGGCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTgg                                              <  1:183873/147‑1 (MQ=255)
                                                                                                   atgATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTgg                                              <  2:1125074/147‑1 (MQ=255)
                                                                                                    aGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGATCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTggg                                             >  1:830460/1‑149 (MQ=255)
                                                                                                    aGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTggg                                             >  1:429385/1‑149 (MQ=255)
                                                                                                     gATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAAAGCTTTCTGGTCTGCCGCAGTGGGc                                            >  1:188633/1‑149 (MQ=255)
                                                                                                       tatatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGGCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCAt                                          <  2:447564/149‑1 (MQ=255)
                                                                                                        atatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATc                                         <  1:614512/149‑1 (MQ=255)
                                                                                                         tatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATAATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTATCTGGTCTGCCGCAGTGGGCATCg                                        >  2:882088/1‑149 (MQ=255)
                                                                                                          atatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCgg                                       <  2:445265/149‑1 (MQ=255)
                                                                                                           tatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATAATATTACAGGGGAGTAGTAATAAGCAACAGTCAAGTTTGGTAGTCATTTTATCAAACGCTATCTGGTCTGCCGCAGAGGCCATCgcc                                      >  2:1182128/1‑147 (MQ=255)
                                                                                                            atCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCt                                     >  2:608052/1‑149 (MQ=255)
                                                                                                             tCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTg                                    >  2:931093/1‑149 (MQ=255)
                                                                                                              cATTTCTACAACCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGa                                   <  1:336985/149‑1 (MQ=255)
                                                                                                                    tACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGt                             <  1:104515/149‑1 (MQ=255)
                                                                                                                     aCAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTa                            >  1:1111546/1‑149 (MQ=255)
                                                                                                                      cAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAg                           >  1:233011/1‑149 (MQ=255)
                                                                                                                      cAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAg                           >  2:1102693/1‑149 (MQ=255)
                                                                                                                       aaaCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGc                          <  1:245238/149‑1 (MQ=255)
                                                                                                                         aCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAg                        >  1:250576/1‑149 (MQ=255)
                                                                                                                         aCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGACTGCGTAGCAg                        >  1:573564/1‑149 (MQ=255)
                                                                                                                          cATACCCGATTCACGGCAGACTCTCAGGATTCTGCCGCTTTTTTATTATTTTTCCGGGGAGTAGTAATGAGCAACTCTGACGGGTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAgg                       <  1:656483/149‑1 (MQ=255)
                                                                                                                           aTACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAggg                      <  2:472431/149‑1 (MQ=255)
                                                                                                                             aCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGcc                    <  1:26215/149‑1 (MQ=255)
                                                                                                                               ccGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                  >  2:580108/1‑149 (MQ=255)
                                                                                                                                 gATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTACTGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTg                <  1:1161266/149‑1 (MQ=255)
                                                                                                                                 gATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTg                <  2:849150/149‑1 (MQ=255)
                                                                                                                                 aaaaagcGGCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTg                >  2:1103415/7‑149 (MQ=255)
                                                                                                                                        ggCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTCGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGACATTGGTATCTg         >  1:719819/1‑149 (MQ=255)
                                                                                                                                           aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTGCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCg      <  1:171128/149‑1 (MQ=255)
                                                                                                                                            gAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTGTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg     <  2:361942/149‑1 (MQ=255)
                                                                                                                                               tctcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAt  <  1:404380/149‑1 (MQ=255)
                                                                                                                                               tctcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAt  >  2:404057/1‑149 (MQ=255)
                                                                                                                                               tctcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGGGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAt  <  2:233011/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAT  >  NC_000913/2775357‑2775648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: