Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,834,234 G→T 7.1% G108V (GGT→GTT)  norW → NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,834,2340GT 7.1% 176.5 / 3.2 73G108V (GGT→GTT) norWNADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (32/35);  new base T (4/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGC  >  NC_000913/2834087‑2834381
                                                                                                                                                   |                                                                                                                                                   
ttACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGt                                                                                                                                                    <  2:1042855/149‑1 (MQ=255)
  aCCCGCCCGACGGCGGGTGAATTTGCAGAGCAGTTTAATCTGCACCTGTTTCCACATACCTGGGTGACGGATATCGATGCCGACGCCCGTGTCGGGAAACGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCACCCGTTGc                                                                                                                                                  >  2:329073/1‑149 (MQ=255)
  aCCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTTCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGc                                                                                                                                                  <  1:295964/149‑1 (MQ=255)
  aCCCGCCAGACGGCGGGTGAAATTGCCGAGCAGTTTAATCTGCACCTGTTTCGACAAACCTCGCTGACGGATATCGATGCCCAAGCCCGTGTGGTCAAAAGCCAGAATAATCACTGGCAATACGACAAGCAACTACTGGCAACCGGTGc                                                                                                                                                  >  1:583233/1‑149 (MQ=255)
    ccGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGCCAAGCTAGTACTGGCCCCCGGTGccc                                                                                                                                                >  1:48771/1‑148 (MQ=255)
    ccGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAACCCCGTGTGGTGAAAAGCCAGAATATTCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCa                                                                                                                                                >  2:82888/1‑149 (MQ=255)
           aCGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCACCGGTTGCCAGtactag                                                                                                                                         <  1:54604/149‑6 (MQ=255)
           aCGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCtt                                                                                                                                         >  2:27686/1‑149 (MQ=255)
            cggcggGTGAATTTGGCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGGGCCTGATATCGATGGCTAAGCCCGTGTGGTGAAAAGCCAGAATAATCATTGGCAATACGACAAGCTAGTACTGGCACCGTTTGCCAGtactagc                                                                                                                                        <  2:383371/149‑7 (MQ=255)
            cggcggGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCttt                                                                                                                                        <  1:45079/149‑1 (MQ=255)
                   tGAATTTGCCGAGCTGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGc                                                                                                                                 <  1:1105191/149‑1 (MQ=255)
                   tGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGTCAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGc                                                                                                                                 <  2:528963/149‑1 (MQ=255)
                          gCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGcc                                                                                                                          >  1:46319/1‑149 (MQ=255)
                             taGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACCGATATCGATGCAGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTgg                                                                                                                       <  1:1103680/148‑1 (MQ=255)
                             gatcAGTTTAATCTGCACCTGTTTCCACACACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGGGCCAGTGCCTTTGTCCCGCCTGTGCCTgg                                                                                                                       <  2:1111512/146‑1 (MQ=255)
                                 aGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGt                                                                                                                   <  1:1075526/149‑1 (MQ=255)
                                 aGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGt                                                                                                                   <  2:63312/149‑1 (MQ=255)
                                   tttAATCTGCACCTGTTTCCACAACCCTGGGTGACGGATATCGATGGCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCGAGTGCCGTTGTCCCGCCTGTGCCTGGGCGTGa                                                                                                                 <  2:28206/149‑1 (MQ=255)
                                   tttAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCTACCGGTGCCAGTGCCTTTGTCCCGACTGTGCCTGGGCGTGa                                                                                                                 <  1:1157602/149‑1 (MQ=255)
                                   tttAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGa                                                                                                                 >  2:973215/1‑149 (MQ=255)
                                          tGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATg                                                                                                          >  2:537069/1‑149 (MQ=255)
                                              cgtGTTTCCACAAACCTGGGTCACGCATATCGATGCCCACCCCCGGGTGGTCAAACGCCACAATAATCCGTGGCAATACCCCAACCTTGTACTGGCAACCGGTGCCAGTGCGTTTG‑CCCGCCTGTGCCTGGGCGTGAGTTAATGCTGAc                                                                                                     <  2:1122643/147‑1 (MQ=255)
                                              ccTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGa                                                                                                      >  1:907201/1‑149 (MQ=255)
                                               cTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCGGAc                                                                                                     >  1:675553/1‑149 (MQ=255)
                                               cTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGGGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGAc                                                                                                     >  2:533845/1‑149 (MQ=255)
                                                tGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACg                                                                                                    <  2:551679/149‑1 (MQ=255)
                                                 gTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCACCCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGt                                                                                                   >  1:349805/1‑149 (MQ=255)
                                                 gTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGt                                                                                                   >  2:316948/1‑149 (MQ=255)
                                                   ttCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTa                                                                                                 >  1:748045/1‑149 (MQ=255)
                                                    tCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTaa                                                                                                <  2:46319/149‑1 (MQ=255)
                                                         aaaCCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGt                                                                                           <  1:565593/149‑1 (MQ=255)
                                                             cTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTCCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGc                                                                                       >  1:761292/1‑149 (MQ=255)
                                                                 gTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAaga                                                                                   >  2:1019090/1‑149 (MQ=255)
                                                                 gTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAaga                                                                                   <  1:1019090/149‑1 (MQ=255)
                                                                 gTGACGGATAACGAAGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGCACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAaga                                                                                   <  2:1073164/149‑1 (MQ=255)
                                                                      ggATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATc                                                                              >  1:1025780/1‑149 (MQ=255)
                                                                          atCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATACTGACGTTAAATAGTCAGCAAGAGTATCgcgc                                                                          <  2:627904/149‑1 (MQ=255)
                                                                           tCGATGCCGAAGCCCGTGTGGGGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGAAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGcc                                                                         <  2:983639/149‑1 (MQ=255)
                                                                              aTGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCtgt                                                                      <  1:513970/149‑1 (MQ=255)
                                                                                 ccGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGGCAGCAAGAGTATCGCGCCTGGGaa                                                                   >  2:1020592/1‑149 (MQ=255)
                                                                                  cGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGaaa                                                                  <  2:295455/149‑1 (MQ=255)
                                                                                       cccGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCaa                                                             <  1:27686/149‑1 (MQ=255)
                                                                                                   aaaGCCAGACTATTCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGGGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCTGGATGcc                                                 <  2:977395/149‑1 (MQ=255)
                                                                                                   aaaGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGcc                                                 >  2:286599/1‑149 (MQ=255)
                                                                                                     aGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCCGTGCCTTTGTCCCGCATGTGCCTGGGCGTGAGTTAATGCTGTCGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGACCg                                               >  1:213267/1‑149 (MQ=255)
                                                                                                        cAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGGGAGTTAATGCTGACGTTAAGTAGTCAGCAAGAGTATCGCGCCTGTGATACGCAACTGCGGGATGCGCGCcg                                            >  2:253934/1‑149 (MQ=255)
                                                                                                         aGAATAATCAGTGGCAATACGACACGCTAGTACTGGCAACCGGGGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGAcgc                                           <  2:599811/149‑1 (MQ=255)
                                                                                                         aGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCcgccgc                                           >  2:1129243/1‑149 (MQ=255)
                                                                                                          taattatGAGTGCCAATACGACACGCTAGTAGTGGGAACCGGTGCCAGTGCCTTTGTCCCGCGTGTGCCTGGTCGTGAGTTAATGCTGACGTTAAATAGTCAGCTAGAGTAACGCGCCTGTGACACGCAACTGCGGGATTCCCGAcgcg                                          <  2:583233/148‑1 (MQ=255)
                                                                                                           aataatCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGGCTTTGTCCCGCCTGTCCCTGGGCGTGAGTTAATGCGGACGTTAACTAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCgt                                         <  2:87541/149‑1 (MQ=255)
                                                                                                             aaatCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCgtgt                                       <  2:384924/148‑1 (MQ=255)
                                                                                                               ttCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTg                                     <  1:1124248/148‑1 (MQ=255)
                                                                                                                tCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGa                                    >  1:495491/1‑149 (MQ=255)
                                                                                                                   gTGGCAATACGACAAGCTAGTACTGGCGGCCGGTGCCAGTGCCTTGGTCCCGGCTGTGCCTGGGCGTGAGTTAATGCTGCCGGTTATTTGTGAGCATGAGTATCGCGCCTGTGGGACGCTACGGCGGGATGCCCGAGGCGTGTTGAttg                                 >  2:68112/1‑149 (MQ=255)
                                                                                                                    tGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGAttgt                                >  2:404453/1‑149 (MQ=255)
                                                                                                                       cAATCCGACAAGCTAGTCCTGGCAACCGGTGCCAGTGCCTTTGGCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTgg                             <  2:862287/149‑1 (MQ=255)
                                                                                                                       cAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTgg                             <  1:283350/149‑1 (MQ=255)
                                                                                                                         aTACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCg                           >  2:936186/1‑149 (MQ=255)
                                                                                                                           aCGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGAAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCggt                         >  2:1102179/1‑149 (MQ=255)
                                                                                                                             gACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGGCTGGGCGAGAGTTAATGATGACGTTAAATAGTCAGCAAGAGTATCGCACCTGTGAAACGCAACTGCGGGATGCCCGACGCCAGTTGAATTTTGGAggttg                       >  1:548049/1‑147 (MQ=255)
                                                                                                                             gACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCggtgg                       >  2:316850/1‑149 (MQ=255)
                                                                                                                               cAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGtt                     >  1:557403/1‑149 (MQ=255)
                                                                                                                                   cTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGAt                 <  1:316948/149‑1 (MQ=255)
                                                                                                                                   cTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGGTTGAt                 <  2:907201/149‑1 (MQ=255)
                                                                                                                                    tAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGAtt                >  1:1051805/1‑149 (MQ=255)
                                                                                                                                    aggcaCTGGCACCGGTTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGAtt                >  2:524568/5‑149 (MQ=255)
                                                                                                                                     ggcaCTGGCACCGGTTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTg               >  1:161015/4‑149 (MQ=255)
                                                                                                                                     aGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGCCGCGTGTTGATTGTTGGCGGTGGTTTGATTg               >  2:65513/1‑149 (MQ=255)
                                                                                                                                     aGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTg               >  1:541289/1‑149 (MQ=255)
                                                                                                                                        ccTGGCCACCGGTGCGAGTGCCGTGGTCCCGCCTGTGCCGGGGCGTGAGTTAATGCTGTCGTTAAATAGTCAGCAAGAGTATCGCGCCGGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGTTTGTTGGCGGTGGTTTGAGTGGTa            <  2:1055051/148‑1 (MQ=255)
                                                                                                                                        aCTGGCACCGGTTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCCACGCGTGTTGATTGTTGTCGGTGGTTTGATTGGTa            >  2:298310/1‑149 (MQ=255)
                                                                                                                                             cAACCGGTGCCAGTGCCTTTGTCACGCCTTTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCTGTGGTTTGATTGGTAGCGaa       <  1:533845/149‑1 (MQ=255)
                                                                                                                                                 cGGTGCCAGTGCGTTTGTCCGGCCTGTGCCTGGGGGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTAGGCGGTGGTTTGATTGGTAGCGAACTgg   <  2:349805/149‑1 (MQ=255)
                                                                                                                                                  ggTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGc  <  1:936186/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGC  >  NC_000913/2834087‑2834381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑