Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,326 C→G 8.4% intergenic (‑62/‑198) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3260CG 8.4% 210.4 / 8.7 78intergenic (‑62/‑198)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base C (37/33);  new base G (5/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGT  >  NC_000913/2839185‑2839470
                                                                                                                                             |                                                                                                                                                
tAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcg                                                                                                                                           <  1:304970/149‑1 (MQ=255)
 aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcgg                                                                                                                                          <  2:973955/149‑1 (MQ=255)
   ccATTACCTGAAAGGACGCGGGAAACGGTCGCTTTTGATTCGCCGGCGCGCTTAGGGTCTTCCATAATCGTCGTCTTCATTTTCCTCCCTTTACACGCAATCAACGCAGTGTACTGCTCCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                        <  2:546456/149‑1 (MQ=255)
   ccATTACCTGAAAGCACGCGGGAAACGGGCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                        <  1:666643/149‑1 (MQ=255)
    ccttACCTGAAATCACGCGGCAAACGGGCGCGTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgg                                                                                                                                       <  2:552036/147‑1 (MQ=255)
    cATTACCTGAAAGCACGCGGGACACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgg                                                                                                                                       <  2:556399/149‑1 (MQ=255)
     aTTACCTGAAAGCACGCGGGAAACGGTCGCTTTAGACACCCCGGCGCGCTTCGCCACGTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTAGCCGATTGTCCTTGCACAATcggcgcg                                                                                                                                      >  1:862940/1‑147 (MQ=255)
          cTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATAATTTTCATACATTTACACGCAATCAACGCAGTGTACTGGACCGTTTTCCGAGTGTCCATGCACAATCGGCGGGaaaaa                                                                                                                                 >  1:667746/1‑149 (MQ=255)
            gAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGTAAAata                                                                                                                               >  2:1010011/1‑149 (MQ=255)
              aaGCACGCGGGAAACGGGCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATAtt                                                                                                                             <  2:894630/149‑1 (MQ=255)
                gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACCATCGGCGGGTAAAATATTCa                                                                                                                           >  1:449735/1‑149 (MQ=255)
                gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                           <  1:420552/149‑1 (MQ=255)
                 cACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAg                                                                                                                          <  1:279680/149‑1 (MQ=255)
                  aCGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAgg                                                                                                                         >  1:924621/1‑149 (MQ=255)
                    gcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATAGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATAGTCCTTGAACAATCGGCGGGAAAAATATTCAGGTg                                                                                                                       >  2:30915/1‑149 (MQ=255)
                      gggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAc                                                                                                                     >  2:486534/1‑149 (MQ=255)
                        gAAACTGGCGCTTTTGCAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGGCATCATTTTCCTCACTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAACTATTCAGGTGACCg                                                                                                                   <  2:78650/149‑1 (MQ=255)
                          aaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGt                                                                                                                 >  1:219861/1‑149 (MQ=255)
                          aaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGt                                                                                                                 >  2:40672/1‑149 (MQ=255)
                            cgctCGCTTTTGAAACCCGGGCGAGCATCGCCACTTCCAGCATCGTCGTCATCATTTTGATCCATATACACGCAATAAACGCAGTCTACTGCTCAGTTTGCCGATTGTCATTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGttt                                                                                                               <  2:946423/146‑1 (MQ=255)
                             ggTTGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCACTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTc                                                                                                              <  1:609255/149‑1 (MQ=255)
                             ggTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTc                                                                                                              <  1:253341/149‑1 (MQ=255)
                                cGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGCCCGGTTTcaca                                                                                                           >  2:561465/1‑149 (MQ=255)
                                 gCTTTTGAAACCCCG‑‑GCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                        >  1:521874/1‑149 (MQ=255)
                                  cTTTTGAAACCCCGGCGCGCGTAGCCACTTCAAGCTTCGTCGTCATCATTTTCTTCCCTTTACACGCAATCCACGCAGTGTAGTGCACCGTTTGCCGATTGTCCTTGCACACTCGGCGGGTAAAATATTCAGGTGACCGGTTTCACaaa                                                                                                         <  1:657913/149‑1 (MQ=255)
                                   ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                        <  1:203387/149‑1 (MQ=255)
                                   atttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCATAAat                                                                                                        <  1:159258/148‑1 (MQ=255)
                                       gAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGGCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                                                                                                    <  2:253668/149‑1 (MQ=255)
                                          aCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaa                                                                                                 >  2:1118314/1‑149 (MQ=255)
                                            cccGGCGCGCTTCGCCACTTCCTGCATCGTCGTCATGATTTTCATCCCTTTACACGCACTCAACGCAGTTTACTGCACCGTTTGCAGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt                                                                                               <  1:873021/149‑1 (MQ=255)
                                            cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt                                                                                               <  1:569410/149‑1 (MQ=255)
                                               ggCGCGATTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGaa                                                                                            <  2:357377/149‑1 (MQ=255)
                                                    gcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAAAATAAAAAATGAACAAtt                                                                                       >  1:588598/1‑149 (MQ=255)
                                                      ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCa                                                                                     <  1:40672/149‑1 (MQ=255)
                                                      ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATAAAAAAAATGAACAATTCa                                                                                     >  1:1152035/1‑149 (MQ=255)
                                                        cGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAct                                                                                   >  1:1157925/1‑149 (MQ=255)
                                                              taccAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGAGTACTGCAACGTTTGCCGATTGACCTTGCACAATCGGCCGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCttc                                                                             >  1:397604/3‑148 (MQ=255)
                                                                   gCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGCCCGGTTTCACAAATATTAAAAATGACCAATTCACTCTCTTGCTTAt                                                                        >  2:224414/1‑149 (MQ=255)
                                                                     aTCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCGTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTAttt                                                                      >  1:67169/1‑149 (MQ=255)
                                                                       cgtcgtCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAg                                                                    <  2:924621/149‑1 (MQ=255)
                                                                           gtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGCCCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTAGCTGATTTAGTGAc                                                                >  2:98714/1‑149 (MQ=255)
                                                                              atcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                             >  1:676016/1‑149 (MQ=255)
                                                                                    ttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCa                                                       <  2:521874/149‑1 (MQ=255)
                                                                                     tCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCAt                                                      <  2:1157925/149‑1 (MQ=255)
                                                                                        tCCCATTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGTAAAAATATTCAGGTGACCGGTATCACAAATATAAAAAATCAACAAATCACTCTCTTGCTGATGAAGGGACGACTATTAATGaa                                                   >  2:781627/1‑148 (MQ=255)
                                                                                         cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtt                                                  >  1:440363/1‑149 (MQ=255)
                                                                                         cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAATCACTCTCTTGCTTATTTAGTGAAAACTATTCATGAtt                                                  >  1:902236/1‑149 (MQ=255)
                                                                                         cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGCAAAAATATTCAGGTGACCGGTTTCACAAATATATAAAATGAACAATTCAGTCTCTTGCTTATTTAGTTACAACTATTAATGAtt                                                  >  1:352651/1‑149 (MQ=255)
                                                                                           cTTTACACGCAATCAACGCAGTGTACTTCAACGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtttt                                                <  1:618964/149‑1 (MQ=255)
                                                                                             ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgt                                              >  1:374891/1‑149 (MQ=255)
                                                                                              tACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                             <  1:561465/149‑1 (MQ=255)
                                                                                               acacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTTCTGACAACTATTCATGATTTTGTGa                                            >  1:97725/1‑149 (MQ=255)
                                                                                                 acGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCCCTCTCTTGCTTTTTTAGTGACAACTATTCATGATTTTGTGaaa                                          >  1:368488/1‑149 (MQ=255)
                                                                                                   gCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                        >  1:900824/1‑149 (MQ=255)
                                                                                                     aaTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCgg                                      <  1:883999/149‑1 (MQ=255)
                                                                                                      aTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGGACAATCGGCGGGAAAAATATTCAGCTGACCGGAGACACAAAGAGACACAATGAACAATTGACTCTCTTGATTATTAATTGCAAAGTATTCATGAATTTGTGACACCGGt                                     >  2:547114/1‑149 (MQ=255)
                                                                                                        ccaCGCAGTGTTCTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                   <  1:125061/147‑1 (MQ=255)
                                                                                                         aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTATTGCTTATTTAGTGACAACTATTAATGATTTTGTGATCACGGttta                                  >  1:1088078/1‑148 (MQ=255)
                                                                                                         aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCGTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGAGTCCCAAATATAAAAAATGAACAATTCACTCTCTTGCTTAGTTAGTGACATCTATTCATGATTTTGTGAAACCGGTTTc                                  >  1:1083411/1‑149 (MQ=255)
                                                                                                         aaCGCAGTGTACTGAACCGTTTGCCGATTGTCCTTGCACAATCGGCGGTAAAAATATTCAGGTGACCGGTTTCACAAAAATAAAAAATTAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                  <  1:940531/149‑1 (MQ=255)
                                                                                                          aCGCAGGGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                 <  2:420891/149‑1 (MQ=255)
                                                                                                            gCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTa                               >  2:165341/1‑149 (MQ=255)
                                                                                                             cAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTaa                              >  2:47528/1‑149 (MQ=255)
                                                                                                                     tGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt                      >  2:487443/1‑149 (MQ=255)
                                                                                                                     tGCACCGTTTGCCGATTGTCCTTGCACAATCGGAGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCAATTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTACTAAATTCCCttt                      >  1:360590/1‑149 (MQ=255)
                                                                                                                      gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTc                     <  2:348099/149‑1 (MQ=255)
                                                                                                                      gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTc                     >  1:472207/1‑149 (MQ=255)
                                                                                                                            tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCTGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCATGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATc               <  2:862940/149‑1 (MQ=255)
                                                                                                                              cgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCgg             >  2:1021104/2‑149 (MQ=255)
                                                                                                                               gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTAACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCgga            >  2:1159164/1‑148 (MQ=255)
                                                                                                                                ccGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa           >  1:892292/1‑149 (MQ=255)
                                                                                                                                ccGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTCGCTTATTTAGTGCCAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa           >  1:323984/1‑149 (MQ=255)
                                                                                                                                ccGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa           >  1:304933/1‑149 (MQ=255)
                                                                                                                                  gATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCATGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCATAATTCCGATTCAGCATCGGCAtt         >  2:44007/1‑149 (MQ=255)
                                                                                                                                   aTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAttt        <  2:1129056/149‑1 (MQ=255)
                                                                                                                                       tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc    <  1:614170/149‑1 (MQ=255)
                                                                                                                                       tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc    <  2:507092/149‑1 (MQ=255)
                                                                                                                                         cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAATAAATGAACAAGTCACTCTCGTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTACGTTTCAGCATCGGCTTTTTTCCGt  >  2:118205/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                
TAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGT  >  NC_000913/2839185‑2839470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: