Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,150 C→T 6.8% intergenic (+162/+28) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1500CT 6.8% 228.8 / 5.7 89intergenic (+162/+28)sibC/serAsRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (33/49);  new base T (3/3);  total (36/53)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGT  >  NC_000913/3057007‑3057297
                                                                                                                                                |                                                                                                                                                   
tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGCATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                                                                                >  2:762463/1‑149 (MQ=255)
tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGccc                                                                                                                                                <  1:345458/149‑1 (MQ=255)
tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                                                                                <  2:1012172/149‑1 (MQ=255)
  ccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAAAGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                                                                              >  1:174174/1‑149 (MQ=255)
  ccctgccATCGAAAATCCCCATTAAATAAACACCACGCATTGATATGACATTGATTTATATTCTGCAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                                                                              <  1:774610/149‑1 (MQ=255)
      gccATCGTAAATCCCCATTAAATAAACACACCGCCTTGCTCTGACTTTGCTTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGGGGAGCAGGGATAAAAAAACGGGCAGGGCACTGACTTGCCCGttttt                                                                                                                                          <  1:755397/149‑4 (MQ=255)
      gccATCGTAAATCCCCATTAAATAAACACAACGCAGTGATCTGACTTTGAGTTATTTTCTGGAGCAGACTCGCAAAGGAGAATGCGCACCGCGGCAACGGTGAGGAGAAGGGATAAAAAAACGGGCAGGGCACTGACTTGCCCGttttt                                                                                                                                          <  1:498620/149‑4 (MQ=255)
        cATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGCCCTGCCCGTTGAttt                                                                                                                                        >  2:115788/1‑149 (MQ=255)
        cATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                                                                        >  2:153022/1‑149 (MQ=255)
         aTCGTAAATCCCCATTAAATAATCACAACGCATTGATCTGACATTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCATCGCGGCAACTGTGTGGAGAACTGTTAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttgt                                                                                                                                       >  1:697048/1‑147 (MQ=255)
           cGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                                     <  2:380281/149‑1 (MQ=255)
           cGTAAATCCCCATTAAATAAACACAACGCATTGATCTGAAGTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                                     <  2:582180/149‑1 (MQ=255)
            ttAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                                                                    <  1:1173850/148‑1 (MQ=255)
            gTAAATCCCCATTAAAAAACCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGACTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                                                                    <  1:1178262/149‑1 (MQ=255)
                  ccccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                                                                              <  2:48575/149‑1 (MQ=255)
                   cccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAggg                                                                                                                             <  1:1099977/149‑1 (MQ=255)
                    ccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCAGTTGATTTTCAGAGAAgggg                                                                                                                            >  2:14375/1‑149 (MQ=255)
                    ccATTAAATAAACACAACGCATTGATCCGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                                            <  1:1132006/149‑1 (MQ=255)
                     cATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                                                                           <  2:387339/149‑1 (MQ=255)
                           ataaaCACAACGCATTTATCTGACTTTGATTTATTTTCTGGAGCAGACACGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGCAAAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGt                                                                                                                     >  1:552806/1‑149 (MQ=255)
                              aaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                                                                                  <  2:1034508/149‑1 (MQ=255)
                               acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAccg                                                                                                                 >  2:220731/1‑147 (MQ=255)
                               acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                                                                                 >  1:1035691/1‑149 (MQ=255)
                                aacaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGTATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                                                                                <  1:816529/148‑1 (MQ=255)
                                       cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                                                                                         <  1:228462/149‑1 (MQ=255)
                                        aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgc                                                                                                        >  1:388220/1‑149 (MQ=255)
                                          tGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcgc                                                                                                      <  1:390689/149‑1 (MQ=255)
                                             tCTGACTTTGATTTTTTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACTGTCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGTGGAATTAGTACAGCAGACGGGCGCGaa                                                                                                   <  2:495794/149‑1 (MQ=255)
                                                 aCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGt                                                                                               >  1:1122259/1‑149 (MQ=255)
                                                   tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                                   <  2:565853/127‑1 (MQ=255)
                                                   tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                                   >  1:565853/1‑127 (MQ=255)
                                                   tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAc                                                                                             >  1:1150512/1‑149 (MQ=255)
                                                    tttatttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAcc                                                                                            <  1:471786/146‑1 (MQ=255)
                                                     tGATGTATTTTCTCGAGCAGACTCCCAAAGTAGAATCCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAATCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGCGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAccc                                                                                           <  2:54461/149‑1 (MQ=255)
                                                      tatttatttTCTGGAGCAGACTCGCAAAGTCGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGTAATTAGTACAGCAGACGGGCGCGAATGGTACCCg                                                                                          <  2:68200/148‑1 (MQ=255)
                                                      gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCg                                                                                          <  2:1122259/149‑1 (MQ=255)
                                                      gATTTATTTTCTGGAGCAGACTCGCAAAGAAGAATGCGCAACGCGGCAACGGGGTGGAGAAGGGATAAAAAAACGGGCAAGGCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCg                                                                                          <  1:288338/149‑1 (MQ=255)
                                                       atttatttTCTGGAGCAGCCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGTTAAAAAAACGGGCAAGTCAGTGACCTGCGCGGTGATTTTCAGAGAAGGGGATGTAGTACAGCAGCCGGGCGCGAATGGTACCCgg                                                                                         >  1:880129/1‑149 (MQ=255)
                                                       atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAATCGGGCAAGTCAGTGCCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGGAGACGGGCGCGAATGGTACCCgg                                                                                         >  1:1061903/1‑149 (MQ=255)
                                                       atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                                                                                         >  2:236018/1‑149 (MQ=255)
                                                                   gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAt                                                                             >  1:125516/1‑149 (MQ=255)
                                                                    atcAGACTCGCAAAGTAGACTGCTCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAACTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                                                                            <  2:813921/147‑1 (MQ=255)
                                                                      cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc                                                                          <  2:692557/149‑1 (MQ=255)
                                                                      cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCAGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc                                                                          >  2:760018/1‑149 (MQ=255)
                                                                      cAGACGCTCAACGTAGAATGCGCAACGCGGCAACGGTGTGGATAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc                                                                          <  2:293957/149‑1 (MQ=255)
                                                                       aGACTCGCATAGTAGAATGCGCACCGCGGCATCGGTGTGGAGATGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGTCGCGAATGGTACCCGGTATAGCTTTCATTGcc                                                                         <  1:543868/149‑1 (MQ=255)
                                                                       aGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGAGTGGAGAAGTGATAAAAAAACGCTCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                                                                         <  1:672406/149‑1 (MQ=255)
                                                                        gACTCGCAAAGTAGCATGCGCAACGCGGCAACGGTGTTGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATATTCAGAGATGGGGAATTAGTACAGGAGACGGGCGCGTATGGTAACCGGAATAGCTTTCATTGCCt                                                                        >  1:661972/1‑149 (MQ=255)
                                                                        caCTCGCACAGGAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAACCGGGCACGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt                                                                        <  1:852388/148‑1 (MQ=255)
                                                                         aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGATGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGTTTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                                       <  1:770677/149‑1 (MQ=255)
                                                                         aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                                       <  1:55316/149‑1 (MQ=255)
                                                                         aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                                       <  1:492924/149‑1 (MQ=255)
                                                                          ctctCAAAGTAGAATGCGCATCGCGGCAACGTTGTGGAGTAGGGATTAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGTAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc                                                                      <  1:1186117/149‑1 (MQ=255)
                                                                           tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa                                                                     <  2:469819/149‑1 (MQ=255)
                                                                             gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                                                                   <  2:1035691/149‑1 (MQ=255)
                                                                                aaGTAGAATGCGCAACGCGGCAACCGTGTGGACAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCt                                                                <  2:667678/149‑1 (MQ=255)
                                                                                     gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCCGCAGCGCTTTTTc                                                           <  1:414728/149‑1 (MQ=255)
                                                                                      aaggtgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAACCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCg                                                          <  1:521460/144‑1 (MQ=255)
                                                                                      aaTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGATTTAGTACAGCAGACGGGCGCGAATGGAACCCGGAATAGCTTTCATTGCCTGCAGCGCTTGTTCg                                                          >  2:567772/1‑149 (MQ=255)
                                                                                      aaTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCg                                                          >  2:563322/1‑149 (MQ=255)
                                                                                          cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCaa                                                      >  1:932923/1‑149 (MQ=255)
                                                                                           gcAACGCGGCAACGGGGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTGCAGAGAAGGTGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGTCTTAGCTTTCTTTGCCTGCTGCGCTTTTTCGGCAAc                                                     >  1:666176/1‑149 (MQ=255)
                                                                                            cAACGCGGCAACTGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACg                                                    <  2:748500/149‑1 (MQ=255)
                                                                                               cgcgGCAAGGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCt                                                 <  1:335637/149‑1 (MQ=255)
                                                                                                  ggCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTTCCTGCAGCGCTTTTTCGGCAACGTCTtcg                                              >  1:943361/1‑149 (MQ=255)
                                                                                                   gCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTACAGAGAAGGGGAATTAGTACAGCAGACGGGCCCGAATGGAACCCGGAATAGCTTTCATTGCCTGCAGCGATTATTCGGCAACGACTtcgt                                             >  2:563561/1‑149 (MQ=255)
                                                                                                         gtgtGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGTAATTTGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                                       <  2:174174/149‑1 (MQ=255)
                                                                                                          tgtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCTGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtta                                      >  2:1006504/1‑148 (MQ=255)
                                                                                                             ggAGAACGGATAAAAAAACGGGCAAGTCAGTGACGTGGCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGGGCTTTTTCGGCAACGTCTTCGTCGGCTtcaat                                   <  1:800325/149‑1 (MQ=255)
                                                                                                              gagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaata                                  <  1:742683/149‑1 (MQ=255)
                                                                                                                gaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatc                                <  1:153022/149‑1 (MQ=255)
                                                                                                                  aGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaa                              <  1:14375/149‑1 (MQ=255)
                                                                                                                   gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat                             >  2:52088/1‑149 (MQ=255)
                                                                                                                   gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat                             <  2:552806/149‑1 (MQ=255)
                                                                                                                   gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTGCGTCGGCTtcaatatcaat                             >  2:1098501/1‑149 (MQ=255)
                                                                                                                       aaaaaaaaCGGCGCAAGGCCGTCACCTGCCCGTTGATTTTCAGAGCAGGGGAATTAGAACAGCAGACGGGCGCGATTGGTTCCCGGAATAGCTTTCATTGGCTGCAGCGCTTTTTCGGCATCGGCTTCGTCGGCTTCAATATCAATAAc                          <  2:333038/148‑1 (MQ=255)
                                                                                                                          aaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACcaca                      >  2:866704/1‑149 (MQ=255)
                                                                                                                            caaCGGGCAGGTCACTGACTTGCACGTTGATTTTCAGAGAAGGGGAATAACTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCACCGTATTCGTCGGCTTCAATATCAATAACcacaga                    >  1:241352/2‑147 (MQ=255)
                                                                                                                             aaCGGGCAAGTCAGAGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGCCGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCGTCAATATCAATAACCACATaa                   >  1:55943/1‑149 (MQ=255)
                                                                                                                              aCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAAc                  <  2:152446/149‑1 (MQ=255)
                                                                                                                                gggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAAccc                <  2:2617/149‑1 (MQ=255)
                                                                                                                                   gCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCAt              >  2:1109839/1‑149 (MQ=255)
                                                                                                                                   gCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCGTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATTACCCAt              >  2:1105855/1‑149 (MQ=255)
                                                                                                                                    cAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATc             >  1:1035508/1‑149 (MQ=255)
                                                                                                                                      aGTCAGTGACCGGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTg           <  2:1087845/149‑1 (MQ=255)
                                                                                                                                      aGTCAGTGACCAGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTg           <  1:760018/149‑1 (MQ=255)
                                                                                                                                       gTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTgg          <  1:931419/149‑1 (MQ=255)
                                                                                                                                       gTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCATCAATATCAATAACCACATAACCCATCTgg          >  2:195517/1‑149 (MQ=255)
                                                                                                                                              aCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAg   >  2:437876/1‑149 (MQ=255)
                                                                                                                                               ccTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGt  >  1:382942/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGT  >  NC_000913/3057007‑3057297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: