Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,146,764 C→A 9.8% intergenic (‑27/+92) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,146,7640CA 9.8% 202.6 / 12.1 87intergenic (‑27/+92)yghW/yghXDUF2623 family protein/pseudogene, dienlactone hydrolase family
Reads supporting (aligned to +/- strand):  ref base C (35/43);  new base A (2/7);  total (37/50)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

TGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGG  >  NC_000913/3146616‑3146911
                                                                                                                                                    |                                                                                                                                                   
tgtgAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTc                                                                                                                                                     <  2:307563/149‑1 (MQ=255)
 gtgAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCa                                                                                                                                                    <  1:185829/149‑1 (MQ=255)
     ttAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATTCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCatata                                                                                                                                                <  2:884637/148‑1 (MQ=255)
      tAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCatatat                                                                                                                                               >  2:605777/1‑149 (MQ=255)
        gCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAg                                                                                                                                             <  1:188464/149‑1 (MQ=255)
         ccTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGc                                                                                                                                            <  1:382189/149‑1 (MQ=255)
         ccTAATACAAAAACGCGTGTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGc                                                                                                                                            <  1:871961/149‑1 (MQ=255)
           tAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCATATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGa                                                                                                                                          <  1:1024893/149‑1 (MQ=255)
           tAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCATATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGa                                                                                                                                          <  1:1029579/149‑1 (MQ=255)
             aTACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGata                                                                                                                                        >  1:426224/1‑149 (MQ=255)
               aCAAAACCGCGATTATAATCGGCACAGAATTTTGTCACATTAACCCCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTATGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAtt                                                                                                                                      <  2:1149270/149‑1 (MQ=255)
                  aaaCCTCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCTTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTa                                                                                                                                   <  2:464689/149‑1 (MQ=255)
                  aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTa                                                                                                                                   <  1:962497/149‑1 (MQ=255)
                  aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaatgaggt                                                                                                                                   <  1:152606/149‑8 (MQ=255)
                  aaaCCGCGTTTATAATCGGCACAGAATTTTGTAACATTAACCGCACTTTAGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTa                                                                                                                                   <  2:720486/149‑1 (MQ=255)
                    aCCGCGGTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGGCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAgg                                                                                                                                 <  2:556871/149‑1 (MQ=255)
                     ccGCGTTTATAATCGGCACAGAATTTTGGCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                <  2:438139/149‑1 (MQ=255)
                      cgcgTTTATAATCGGCACAGAATTTTGTCACAATAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCa                                                                                                                               <  2:1047757/149‑1 (MQ=255)
                          tttATAATCGGAACAGAATTTTGTCACATTAACCGCACTTTCGCCATGCGTTGCTTTTAATCCCGCAATTAAGACTTTAAAAAAATGGTTATTCATCGTTCACCTCATTCTCTATATATTGTCATATATATCGATATTATAGGCaaaaa                                                                                                                           >  2:883486/1‑147 (MQ=255)
                            tataATCTGCACATAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAAc                                                                                                                         <  1:288064/149‑1 (MQ=255)
                              taATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGa                                                                                                                       <  1:868856/149‑1 (MQ=255)
                               aaTCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGAc                                                                                                                      >  2:709564/1‑149 (MQ=255)
                                  cGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACttt                                                                                                                   <  1:294107/149‑1 (MQ=255)
                                  cGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACttt                                                                                                                   >  1:736576/1‑149 (MQ=255)
                                   ggCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACtttt                                                                                                                  <  1:393646/149‑1 (MQ=255)
                                    gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                 >  2:36145/1‑149 (MQ=255)
                                    gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                 >  1:379620/1‑149 (MQ=255)
                                     cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgc                                                                                                                >  2:433039/1‑149 (MQ=255)
                                        aGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCa                                                                                                             >  2:656076/1‑149 (MQ=255)
                                        aGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCa                                                                                                             >  2:526770/1‑149 (MQ=255)
                                            ttttGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATAAAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCt                                                                                                         >  2:592965/1‑149 (MQ=255)
                                                    caTTAACCGCACTGTCGGCATTCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTc                                                                                                 <  1:386334/149‑1 (MQ=255)
                                                       tAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCCTTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATg                                                                                              <  2:968173/149‑1 (MQ=255)
                                                        aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGc                                                                                             <  2:778775/149‑1 (MQ=255)
                                                        aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGc                                                                                             <  2:151597/149‑1 (MQ=255)
                                                         aCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATATCGATATTTTAGGCAAAGAACGACTTTTTCGCACGCTATTTTTTCATGCt                                                                                            >  2:995560/1‑149 (MQ=255)
                                                          ccGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGCTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGAGATATTTTAGGCTAAGAAAGACTTTTGCGCACGCTTTTTTTTCATGCtt                                                                                           >  1:274429/1‑149 (MQ=255)
                                                              aCTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGttt                                                                                       <  2:118353/149‑1 (MQ=255)
                                                                  tCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGa                                                                                   <  1:283019/149‑1 (MQ=255)
                                                                  tCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTAACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGAGCACGCTATTTTTTCATGCTTGTTTAAGa                                                                                   >  1:703772/1‑149 (MQ=255)
                                                                    ggCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACg                                                                                 <  1:325414/149‑1 (MQ=255)
                                                                       aTGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTaa                                                                              >  1:1163874/1‑149 (MQ=255)
                                                                        tGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAAt                                                                             >  2:206790/1‑149 (MQ=255)
                                                                         gCGATGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATc                                                                            <  2:218690/149‑1 (MQ=255)
                                                                          cGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATcc                                                                           <  1:656076/149‑1 (MQ=255)
                                                                           gTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATccc                                                                          >  1:1023210/1‑149 (MQ=255)
                                                                             tGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGt                                                                        >  1:528480/1‑149 (MQ=255)
                                                                               cTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGCACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTaa                                                                      >  2:615284/1‑149 (MQ=255)
                                                                                      tCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATaa                                                               >  1:624046/1‑149 (MQ=255)
                                                                                        ccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAc                                                             <  1:484180/149‑1 (MQ=255)
                                                                                        ccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAc                                                             <  2:525111/149‑1 (MQ=255)
                                                                                           ccATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTa                                                          <  1:709564/149‑1 (MQ=255)
                                                                                                 aGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGga                                                    <  1:445472/149‑1 (MQ=255)
                                                                                                 aGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGga                                                    <  2:929130/149‑1 (MQ=255)
                                                                                                 aGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGga                                                    >  1:602152/1‑149 (MQ=255)
                                                                                                           aaaaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTa                                          >  1:1041382/1‑149 (MQ=255)
                                                                                                            aaaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTAt                                         >  2:466595/1‑149 (MQ=255)
                                                                                                            aaaTGGTTATTCATAGTTCAACTAATTCGCTATATATTGTAATATATAGCGATATTTTATGCAAAGAACGACTTTTGCGCACGCTATTTTTTCCTGCTTGTTTAAGACGTAATCCCGTAAGATATAAACCTATCAGGAGAGATATTTAt                                         <  1:592436/149‑1 (MQ=255)
                                                                                                               tGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGa                                      <  1:526770/149‑1 (MQ=255)
                                                                                                                 gTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAc                                    >  1:774595/1‑149 (MQ=255)
                                                                                                                     ttCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATACACCTATCAGGAGAGATATTTATCGAACCAtt                                >  1:785118/1‑149 (MQ=255)
                                                                                                                      tCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCACGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAACAttt                               >  1:20014/1‑149 (MQ=255)
                                                                                                                        ttCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCATAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCa                             <  2:734818/148‑1 (MQ=255)
                                                                                                                        aTCGTTCACCTCATTCTGTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCAAGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGCTAAACCTATCAGGAGAGATATTTATCGAACCATTTCa                             <  2:217022/149‑1 (MQ=255)
                                                                                                                        aTCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCa                             <  2:1142590/149‑1 (MQ=255)
                                                                                                                        aTCGTTCACCTCATTCGCTATATATTGTAATATATAGCGATATTATAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCa                             <  1:214574/149‑1 (MQ=255)
                                                                                                                         tCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAg                            <  2:426224/149‑1 (MQ=255)
                                                                                                                           gTTCACCTCATTCGATATATATTGTCATATATAGAGATATTGATGGCACAGAACGACTTTTGCGCACGCTATATTTTCATGCTTGTTTAAGCCGTAATCCCGTACGAGATAAACCTATCAGGCGAGATATTTATCGAACCATTTCAgtg                          <  1:989584/149‑1 (MQ=255)
                                                                                                                            ttaaCCTCATTCGCTATATATTGTCATATATAGCGAGATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGAAATAGAAAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                         >  2:1083670/4‑149 (MQ=255)
                                                                                                                            ttCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                         <  2:379620/149‑1 (MQ=255)
                                                                                                                            ttCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAGTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                         >  2:510139/1‑149 (MQ=255)
                                                                                                                              cACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTcc                       <  1:433039/149‑1 (MQ=255)
                                                                                                                               aCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCt                      >  1:524301/1‑149 (MQ=255)
                                                                                                                                ccgcATTCGCTTTATATTGTCATATATAGCGATATTTTTGTAAAAGAACTACTTTTGCGCACGCTATTTATTCTTGCTTGTTTAAGACGTATTCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGGGTCGtt                     <  2:568843/146‑1 (MQ=255)
                                                                                                                                 ctctTTCGCTATATTTTGTCATATATAGCGATATTTTAGGCAAAGTACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCttt                    <  2:119732/149‑1 (MQ=255)
                                                                                                                                 cTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCttt                    >  1:838363/1‑149 (MQ=255)
                                                                                                                                    aTTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGcc                 >  1:526158/1‑149 (MQ=255)
                                                                                                                                        gCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGc             >  1:664981/1‑149 (MQ=255)
                                                                                                                                        gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGc             >  1:458776/1‑149 (MQ=255)
                                                                                                                                        gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGc             >  1:157269/1‑149 (MQ=255)
                                                                                                                                           atatatTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTAAGTGTCCTTTGCCAGGCAAg          >  2:240598/1‑149 (MQ=255)
                                                                                                                                               atTGTCATATATAGCGATATTTTAGGAAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCACGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcg      <  2:74614/149‑1 (MQ=255)
                                                                                                                                                  gTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCCCGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcg   <  2:129927/149‑1 (MQ=255)
                                                                                                                                                   tCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg  >  1:497504/1‑149 (MQ=255)
                                                                                                                                                   tCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg  <  2:607104/149‑1 (MQ=255)
                                                                                                                                                   tCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg  <  2:115378/149‑1 (MQ=255)
                                                                                                                                                   tCATATATAGCGATATTTTAGGCAAAGAACGACTATTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCATGCAAGAAcggcgg  >  1:900097/1‑149 (MQ=255)
                                                                                                                                                   tCATATATAGCGATAATTTAGGCAAAGAACGACTTTTGGGCACGCTATTTTATCAAGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg  <  2:246126/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGG  >  NC_000913/3146616‑3146911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: