Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,328,183 T→A 5.4% intergenic (+100/+56) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,328,1830TA 5.4% 265.2 / 2.1 90intergenic (+100/+56)yhbY/greARNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
Reads supporting (aligned to +/- strand):  ref base T (49/36);  new base A (2/3);  total (51/39)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGC  >  NC_000913/3328035‑3328330
                                                                                                                                                    |                                                                                                                                                    
ggTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCAt                                                                                                                                                      >  1:527054/1‑149 (MQ=255)
    cTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCg                                                                                                                                                  >  2:207833/1‑149 (MQ=255)
          cGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttt                                                                                                                                            <  1:611617/149‑1 (MQ=255)
           gCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCtttt                                                                                                                                           <  2:1047208/149‑1 (MQ=255)
            cccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt                                                                                                                                          >  2:905567/1‑149 (MQ=255)
            cccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACAAATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCttttt                                                                                                                                          <  2:890845/149‑1 (MQ=255)
             ccAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGTCCGCATAGCGGCCATTTTc                                                                                                                                         >  1:101316/1‑149 (MQ=255)
             ccAACTAAAGAACGTAAAATCTCGATGCCACGCTAAGATTATCCTAATGTTACACACATTTGCTGTTTAAAACGATGGGTTTGCCGAAGGCAGGAGAGCAAAAAGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTc                                                                                                                                         <  2:640178/149‑1 (MQ=255)
              cAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTcc                                                                                                                                        <  1:1034097/149‑1 (MQ=255)
               aaCTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGGTACACACATTTGCTCTGTAAAACGAGGCGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttatca                                                                                                                                       <  1:238747/149‑5 (MQ=255)
                  aaaaGAACGTAAACTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCctttc                                                                                                                                    <  2:79236/148‑1 (MQ=255)
                    aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttatcaa                                                                                                                                      >  1:1105741/1‑140 (MQ=255)
                    aaGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTATGCGGCCttttatcaa                                                                                                                                      <  2:1105741/145‑6 (MQ=255)
                               aTCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGGTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATc                                                                                                                       <  2:891154/149‑1 (MQ=255)
                                 ctcGCTGCCACGCTAAGATTCTCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAACTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCaa                                                                                                                     <  2:1076063/149‑1 (MQ=255)
                                  tcGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAAc                                                                                                                    <  1:453903/149‑1 (MQ=255)
                                      tGCCACGCTAAGATTTTCCGAAAGTTACACACATTTGCTGGGTAAAACGAGGGGGTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCGGTTCGTTGATAAAAGGCCGCATAGGGGCCTGTTAGCTTTCTGTACAAGACATCAACATCt                                                                                                               >  2:1153902/1‑149 (MQ=255)
                                        ccACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTg                                                                                                             >  2:444658/1‑149 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                            <  1:1182606/149‑1 (MQ=255)
                                         cACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGa                                                                                                            <  1:743678/149‑1 (MQ=255)
                                              tAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTAtt                                                                                                       <  2:895859/149‑1 (MQ=255)
                                               aaGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTg                                                                                                      >  2:615592/1‑149 (MQ=255)
                                                 gATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTggg                                                                                                    <  2:182454/149‑1 (MQ=255)
                                                    tATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTaa                                                                                                 <  2:251611/149‑1 (MQ=255)
                                                       ccTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTc                                                                                              >  1:264151/1‑149 (MQ=255)
                                                         tAAAGTTACCCACATTTGCTGTGTAAAACGAGGGGGTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCtt                                                                                            <  1:797680/149‑1 (MQ=255)
                                                         tAAAGTTACACACATTTGCTGTGTAAACCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGACACGCTCTGGTCGTTGATAAAAGGCCGCATAGCGTCATTGTTCCTTTCGTTAACATACATCACCTTCTTGAGTATTGGGTAATTCtt                                                                                            >  2:652456/1‑149 (MQ=255)
                                                         tAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCtt                                                                                            >  1:532899/1‑149 (MQ=255)
                                                         tAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCATTTTGCATTGGTTTACATTACTACACATGATGGAGTATTGGGTAATTCtt                                                                                            >  2:815342/1‑149 (MQ=255)
                                                            aGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACa                                                                                         >  2:177935/1‑149 (MQ=255)
                                                            aGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACa                                                                                         <  2:1017036/149‑1 (MQ=255)
                                                            aGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACa                                                                                         <  2:955919/149‑1 (MQ=255)
                                                             gTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAg                                                                                        <  1:614253/149‑1 (MQ=255)
                                                                 cacacaTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAt                                                                                    >  1:584599/1‑149 (MQ=255)
                                                                      aTTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAc                                                                               <  2:405239/149‑1 (MQ=255)
                                                                       tttGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCAcc                                                                              >  2:15285/1‑149 (MQ=255)
                                                                          gCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTa                                                                           <  2:193232/149‑1 (MQ=255)
                                                                           cTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTaa                                                                          <  1:406909/149‑1 (MQ=255)
                                                                             gtgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAAtt                                                                        >  2:579444/1‑149 (MQ=255)
                                                                              tgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTa                                                                       >  2:21720/1‑149 (MQ=255)
                                                                              tgtAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTa                                                                       >  2:825947/1‑149 (MQ=255)
                                                                                tAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACCGGTTTTCCACCTTAATTaat                                                                     >  2:1098002/1‑147 (MQ=255)
                                                                                 aaaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACtt                                                                    >  1:1114735/1‑149 (MQ=255)
                                                                                  aaaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTc                                                                   >  1:209449/1‑149 (MQ=255)
                                                                                   aaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCTTAGCGGCCTTTTTCCTGTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTGTTACAGGTATTCCACGTTAATTACTTCa                                                                  >  1:830145/1‑149 (MQ=255)
                                                                                   aaCGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCa                                                                  >  2:986000/1‑149 (MQ=255)
                                                                                     cGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCaaa                                                                <  2:228490/149‑1 (MQ=255)
                                                                                        ggggTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTc                                                             >  2:423943/1‑149 (MQ=255)
                                                                                           gTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTaa                                                          >  2:608863/1‑148 (MQ=255)
                                                                                           gTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTAc                                                          >  1:490483/1‑149 (MQ=255)
                                                                                           gTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTAc                                                          <  2:27799/149‑1 (MQ=255)
                                                                                           gTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTAc                                                          <  1:491768/149‑1 (MQ=255)
                                                                                             tttCAGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACtt                                                        <  1:96675/149‑1 (MQ=255)
                                                                                                ccGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTcgc                                                     >  1:30953/1‑149 (MQ=255)
                                                                                                ccGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATACTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTcgc                                                     >  1:297422/1‑149 (MQ=255)
                                                                                                     ggcaggAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGccc                                                <  1:905567/149‑1 (MQ=255)
                                                                                                          gagaGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGt                                           >  1:507371/1‑149 (MQ=255)
                                                                                                           agagCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGtt                                          >  2:850352/1‑149 (MQ=255)
                                                                                                            gagCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGttt                                         <  2:527054/149‑1 (MQ=255)
                                                                                                               cAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCTCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGCGTAATTCTTACAGGTATACAACCTTAATTACTTCAAATTATACTTCGACGACCGGCGTTTtga                                      >  1:118692/1‑149 (MQ=255)
                                                                                                               cAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                      >  2:1019113/1‑149 (MQ=255)
                                                                                                               cAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTGTCCTTTCTTTACAATACATCAACATCTTGAGTAGTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtga                                      >  1:654893/1‑149 (MQ=255)
                                                                                                                aaaaTTCCACGCTCTGTTCGTTGCTAAACGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTACTTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                     <  1:647796/149‑1 (MQ=255)
                                                                                                                aaaaTGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTtgat                                     >  1:586487/1‑149 (MQ=255)
                                                                                                                    tGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGAcc                                 <  1:207833/149‑1 (MQ=255)
                                                                                                                     gCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACca                                >  2:925834/1‑149 (MQ=255)
                                                                                                                      ccACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACcac                               <  2:264151/149‑1 (MQ=255)
                                                                                                                       cACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGATTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACcaca                              >  1:376159/1‑149 (MQ=255)
                                                                                                                       cACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACcaca                              >  1:937682/1‑149 (MQ=255)
                                                                                                                       cACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACcaca                              <  2:1114735/149‑1 (MQ=255)
                                                                                                                          gCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCacaaca                           <  2:294421/149‑1 (MQ=255)
                                                                                                                          gCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCacaaca                           >  1:1139211/1‑149 (MQ=255)
                                                                                                                           ctctGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcat                          >  1:430197/1‑149 (MQ=255)
                                                                                                                                ttCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtct                     >  2:261344/1‑149 (MQ=255)
                                                                                                                                  cGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttc                   <  1:608303/149‑1 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATCCTACTTCGCCGCCCGGCGttt                                         >  1:605413/1‑126 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATCCTACTTCGCCGCCCGGCGttt                                         <  2:58263/126‑1 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATCCTACTTCGCCGCCCGGCGttt                                         >  1:58263/1‑126 (MQ=255)
                                                                                                                                   gTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATCCTACTTCGCCGCCCGGCGttt                                         <  2:605413/126‑1 (MQ=255)
                                                                                                                                     tGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGTCCTCAACATCAtcttcttg                >  1:508843/1‑148 (MQ=255)
                                                                                                                                      gATAAAAGGCCGCATAGCGGCCTTGTTCCTTTCTTTACAATACATCAACATCTTGAGTAGTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACACCATCAgctgctgct               >  1:272019/1‑149 (MQ=255)
                                                                                                                                       gaaaaaGGCCGCTATGCGGCCTTTTTCCTTTCTTAACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTAAATTAATTCATATTCTACTTCGCCGCCCGCCGTTTAGATGACCACAACATAATctactactt              >  1:800006/3‑149 (MQ=255)
                                                                                                                                       aTAAAAGGCCGCATAGCGGCGTGTTTCGTTTCTTTACAATACATCAAGATCTTCAGTATGTGGTAATTGTTACTGGTTTTGCACCTTTATTACTTCAAATTCTACTACGCCCACCGGCGTGTTGTTGACGACGACTTCATctgcttctt              >  2:328641/1‑149 (MQ=255)
                                                                                                                                        tAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCttt             >  2:535365/1‑149 (MQ=255)
                                                                                                                                         aaaaGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCGTAATTACTGCAAATTCTACTTAGCCGCCCGGCGTTTTGATGACCACAACATCATATTCTTCTTTg            >  2:529235/1‑149 (MQ=255)
                                                                                                                                           aaGGCCGCATACCGGCCATTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGcc          >  2:885788/1‑149 (MQ=255)
                                                                                                                                              gCCGCATATCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt       <  1:779735/149‑1 (MQ=255)
                                                                                                                                                cGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTTCCGATCa     >  2:114305/1‑149 (MQ=255)
                                                                                                                                                 gCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAg    >  2:918942/1‑149 (MQ=255)
                                                                                                                                                  cATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAgg   <  2:937682/149‑1 (MQ=255)
                                                                                                                                                   aTAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTAGTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGc  >  1:707492/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTGCTTTATCGCCCAACTAAAGAACGTAAAATCTCGCTGCCACGCTAAGATTATCCTAAAGTTACACACATTTGCTGTGTAAAACGAGGGGTTTTCCGCAGGCAGGAGAGCAAAATGCCACGCTCTGTTCGTTGATAAAAGGCCGCATAGCGGCCTTTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGC  >  NC_000913/3328035‑3328330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: