Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,832,797 T→G 6.8% N581H (AAC→CAC)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,7970TG 6.8% 364.6 / 11.7 146N581H (AAC→CAC) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (72/63);  new base G (8/3);  total (80/66)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 149 total aligned reads displayed.

GGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAA  >  NC_000913/3832650‑3832945
                                                                                                                                                   |                                                                                                                                                    
ggTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTc                                                                                                                                                     <  1:48374/149‑1 (MQ=255)
ggTAGAACTGCACATCGCCCGCTTCACTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGGCCGGGAACTCCATCATCATGGCCCGCATCCTCGGCGTACCCCGCGCGTTc                                                                                                                                                     <  1:894883/149‑1 (MQ=255)
     aaCTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCAcg                                                                                                                                                >  1:358373/1‑149 (MQ=255)
      aCTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCAcgc                                                                                                                                               <  2:837585/149‑1 (MQ=255)
       cTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCAcgcg                                                                                                                                              >  2:919866/1‑149 (MQ=255)
        tGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACgcgc                                                                                                                                             >  2:1010329/1‑149 (MQ=255)
        tGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACgcgc                                                                                                                                             >  1:1170589/1‑149 (MQ=255)
         gCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTGCGAACgcgcg                                                                                                                                            <  2:535754/149‑2 (MQ=255)
           acaTCGCCCGCTTCAGGGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACTCCGCGCGCTCGCACGCGCAGc                                                                                                                                          <  1:857613/149‑1 (MQ=255)
             aTCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTTTTGACGGTCAAGGTAATCACCAGCCGGAGCGGCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCtt                                                                                                                                        <  1:675639/149‑1 (MQ=255)
             aTCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCtt                                                                                                                                        >  2:434714/1‑149 (MQ=255)
              tCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTc                                                                                                                                       >  2:287190/1‑149 (MQ=255)
               cGCCCGCTGCAGTGAACACCGGCGCAACCATCACGTGGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCACGCATCATCGGCGTACCCCGCGCGTGCGCACGCGCAGCTTCa                                                                                                                                      <  2:894149/149‑1 (MQ=255)
                gCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCAc                                                                                                                                     <  1:695050/149‑1 (MQ=255)
                 cccGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACg                                                                                                                                    <  2:1082020/149‑1 (MQ=255)
                 cccGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACg                                                                                                                                    >  1:965830/1‑149 (MQ=255)
                      ttCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGAAACATCGGCGTACCCCGCGCGATCGCACGCGCAGCTACACGATACa                                                                                                                               >  1:1081163/1‑149 (MQ=255)
                        cAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATAATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGa                                                                                                                             >  1:1104123/1‑149 (MQ=255)
                         aGTGAACACCTGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCAAGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCAGGATACCGAt                                                                                                                            >  2:97334/1‑149 (MQ=255)
                          gTGAACACCGGCGCAACCATCACGGTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGCTCGCACGCGCAGCTTCACGATACAGATa                                                                                                                           <  1:225801/149‑1 (MQ=255)
                          gTGAACACCGGCGCAACCATCAAGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCCCATCATCGGCGTACCCCGCGCGGTCGCACGCGCAGCTTCCCGATACAGaaa                                                                                                                           >  2:593596/1‑147 (MQ=255)
                           tGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATAc                                                                                                                          >  1:936333/1‑149 (MQ=255)
                           tGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATAc                                                                                                                          >  1:1009356/1‑149 (MQ=255)
                            gAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACg                                                                                                                         >  1:610684/1‑149 (MQ=255)
                            gAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACg                                                                                                                         >  1:63661/1‑149 (MQ=255)
                                acCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCAGCATCATGGCCCGCATCTTCGGCGTACCCCGCGCGTTCGCACGCGCAGCGTCAGGCTACCGATCCGGcat                                                                                                                     >  2:83840/1‑149 (MQ=255)
                                   ggCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGcatcat                                                                                                                  <  1:1058701/149‑1 (MQ=255)
                                    gcgcCACCATCACGTTGTCGCCTAACATGTATTGAAGGTCAAGGTAATCCCAAGCCGGATCGGCCGGGAACTCCAGCATGATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGAGACAGATACGGCATCATg                                                                                                                 >  1:770015/1‑149 (MQ=255)
                                      gcAACCATCACGTTGTCGCCTAACATGTAGTGACGGTCAAGGTAATCCCAAGCCGGAGCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCCCGCGCAGCGTCCCGATACAGGTACGGCATCATGCg                                                                                                               >  2:790867/1‑149 (MQ=255)
                                       cAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCgg                                                                                                              <  1:862864/149‑1 (MQ=255)
                                       cAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCgg                                                                                                              <  2:103884/149‑1 (MQ=255)
                                        aaCCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATCCGGc                                                                                                             >  1:80052/1‑149 (MQ=255)
                                           cATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTGCCGATACAGATACGGCCTCATGCGGCAtt                                                                                                          >  2:322701/1‑149 (MQ=255)
                                           cATAACGTTGTCGCATAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAAGTCCATCATCAATGCCCGCATCATCGGCGTGCCACGCGGGTTCGCACGCGCAGCTTCCCGATACAGCTACGGCTTCATGCGGCAtt                                                                                                          >  2:637387/1‑149 (MQ=255)
                                                 gTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGGTACAGATACGGCATCATGCGGCATTTCAGtt                                                                                                    >  2:1175309/1‑149 (MQ=255)
                                                   ggTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGc                                                                                                  <  2:575380/148‑1 (MQ=255)
                                                     tCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGt                                                                                                <  1:745005/149‑1 (MQ=255)
                                                     tCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGt                                                                                                <  2:741460/149‑1 (MQ=255)
                                                       gCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTga                                                                                              >  1:46291/1‑149 (MQ=255)
                                                       gCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGAGTACCCCGCGCGTTCGCACGCGCAGATTCACGTTACAGATACGGCATCATGCGGCATTTCAGTTGCGTga                                                                                              >  2:1168942/1‑149 (MQ=255)
                                                        ccTACCAGGTATTGCCGGTCAAGGTAATCCCAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCCTCGGCGTACCCCGCGCGTTCGCACGCGCACCTTACCGATAAAGATACGGCCTCCAGCGCCATTTCAGTAGCGTgaa                                                                                             >  2:218634/1‑149 (MQ=255)
                                                         cTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTgaag                                                                                            >  2:935543/1‑149 (MQ=255)
                                                           aaCCTGTATTGACGGTCAAGGCAATCACAATCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTgaagaa                                                                                          <  2:712654/149‑1 (MQ=255)
                                                              aTGTATTGACGGTCAAGGTAATCACAAGCCTGATCGTCCGGGAACTCCATCATCATGGCCAGCATCATCGGCGTACCCCGCGCGTTCGCACGAGCAGCTTCACGATACAGAGACGGCATCATGCGGCATATCAGTTGCGTGAAGAAgcg                                                                                       >  2:1047639/1‑149 (MQ=255)
                                                               tGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAgcgc                                                                                      <  1:273638/149‑1 (MQ=255)
                                                               tGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAgcgc                                                                                      <  1:434714/149‑1 (MQ=255)
                                                                 tATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcac                                                                                    <  1:663411/149‑1 (MQ=255)
                                                                    tGACGGTCACGGTATTCCCTAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCTCATCATCTGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACATATACGTCATCATGCGGCATTTCAGTTGCGTGAAGAAGCTcaccac                                                                                 <  2:661136/149‑1 (MQ=255)
                                                                    tGACGATCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcaccac                                                                                 <  1:626311/149‑1 (MQ=255)
                                                                      aCGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCAATAATCATGGCCCACATCATCGGCGTACCACGCGCGTTCGCACGCGCAGCTTCAAGATACAGATAAGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACAt                                                                               <  1:655621/149‑1 (MQ=255)
                                                                        ggTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATca                                                                             <  1:287190/149‑1 (MQ=255)
                                                                        ggTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCAACATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCCGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACCTca                                                                             >  2:1109636/1‑149 (MQ=255)
                                                                         gTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCCCGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcac                                                                            >  2:676149/1‑149 (MQ=255)
                                                                          tCAAGGTAATCACAAGCCGGATCGTCCGGGAACTGCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcaca                                                                           <  2:93058/149‑1 (MQ=255)
                                                                          tCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTCCCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCCCCACATcaca                                                                           >  2:262957/1‑149 (MQ=255)
                                                                          tCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcaca                                                                           >  1:711036/1‑149 (MQ=255)
                                                                                  aTCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACtcat                                                                   <  2:986679/149‑1 (MQ=255)
                                                                                  aTCACAAACCGGATCGTCCTGGAACTCCATCATCATGTCCCGAATCATCGGCGTACCCCGCGCGGGCGCACGCGCAGATTCACGCTACACCTACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACtcat                                                                   <  2:652851/149‑1 (MQ=255)
                                                                                   tCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGGACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACtcatc                                                                  <  1:1129440/149‑1 (MQ=255)
                                                                                    cacaAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACtcatca                                                                 <  2:1009356/149‑1 (MQ=255)
                                                                                    cacaAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACtcatca                                                                 <  2:936333/149‑1 (MQ=255)
                                                                                      caAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATAGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGAGAAGAAGCGCACCACATCACAGGACTCatcatc                                                               >  1:169222/1‑149 (MQ=255)
                                                                                        aGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGt                                                             <  2:1080944/149‑1 (MQ=255)
                                                                                         gCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCCCATCACAGGACTCATCATCGTa                                                            >  2:66872/1‑149 (MQ=255)
                                                                                          ccGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCTCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAg                                                           <  2:63661/149‑1 (MQ=255)
                                                                                           cGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAgg                                                          <  1:919866/149‑1 (MQ=255)
                                                                                              aTCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCAGTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGccc                                                       >  2:690585/1‑149 (MQ=255)
                                                                                                  tCCGGTAACTCCATCATCATGGCACGCATCATCGGCGTACCGCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGACGCGCACCAGATCACAGGACTCATCATCGTAGGCCCACgg                                                   <  2:740619/149‑1 (MQ=255)
                                                                                                  tCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACgg                                                   <  1:521513/149‑1 (MQ=255)
                                                                                                    cGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGca                                                 <  1:161577/149‑1 (MQ=255)
                                                                                                      ggAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGcaca                                               <  1:1015752/149‑1 (MQ=255)
                                                                                                       gAACTCCATCATCATGGCCCGCATCATAGGCTTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCacac                                              >  2:1133394/1‑149 (MQ=255)
                                                                                                          cTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGAt                                           <  1:608744/149‑1 (MQ=255)
                                                                                                           tCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATa                                          >  1:586316/1‑149 (MQ=255)
                                                                                                             catcatcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAg                                        >  1:102722/1‑149 (MQ=255)
                                                                                                             catcatcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAg                                        >  1:22572/1‑149 (MQ=255)
                                                                                                             catcatcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACGCATCATCGTAGGCCCCCGGCACACGATAAg                                        >  2:488200/1‑149 (MQ=255)
                                                                                                                catcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACCTCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGAtt                                     >  1:1002240/1‑149 (MQ=255)
                                                                                                                 atcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGAttt                                    >  1:248649/1‑149 (MQ=255)
                                                                                                                   catGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGc                                  <  1:437116/149‑1 (MQ=255)
                                                                                                                         ccGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCCTCATGCGGCATTTCAGTTGCGTGAAGCAGCGCACCACATCACAGGACTCATCATCGTAGGCCCCCGGCACACGATAAGATTTGCTACCgt                            >  1:563599/1‑149 (MQ=255)
                                                                                                                          cGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCgtg                           >  2:159090/1‑149 (MQ=255)
                                                                                                                           gCATCATCGGCGTACCCCGCGCGTTCTCACGCTCAGCTTAAAGATACAGATACTGAATCATGCGGCATTTCAGTTGCTTGAAGAATCGCACCACATAACAGGACTCATCATAGTAGGCCCACGGCACCCGATAAGATTTGCTACCgtgt                          >  2:818013/1‑149 (MQ=255)
                                                                                                                               catcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGCTACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAAc                      >  2:703526/1‑149 (MQ=255)
                                                                                                                                 tcGGCGTACCCCGCGCGTTCGCACGCGCATCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCTTCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACgg                    <  1:1168942/149‑1 (MQ=255)
                                                                                                                                 tcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACgg                    <  1:949314/149‑1 (MQ=255)
                                                                                                                                    gCGTACCCCGCGCGTTCGAACGCGCAGCTTCCCGATACAGATACGGCATCATGCGGCATATCACTTGCGTGAAGACGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCCCACGATAAGATTTGCTACCGTCTAAACgacaa                 >  1:669652/1‑145 (MQ=255)
                                                                                                                                       tACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATgg              <  1:480177/149‑1 (MQ=255)
                                                                                                                                          gctgcgcgTGCGAACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGACGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTg           >  1:737660/4‑149 (MQ=255)
                                                                                                                                          gctgcgcgTGCGAACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACCCGATAAGATTTGCTAGCGTGTAAACGGCTATGGCTg           >  2:918532/4‑149 (MQ=255)
                                                                                                                                          gctgcgcgTGCGAACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATAACCGGACTCATAATAGTAGGCAAACGGCACACGATAAGATTTGCTACCGTGTTACCGGCTATGGCTg           >  1:416063/4‑149 (MQ=255)
                                                                                                                                          gctgcgcgTGCGAACGCGCAGCTTAACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATACCAGGACTCATAATCGTAGGCCCACGGCAAACGATAAGAGTTTCTAACGTATAAACTGCTATGGCTg           >  1:421187/4‑149 (MQ=255)
                                                                                                                                          cccGCGCGTTCGCACGCGCAGCTTAACTATACAGATAAGGCATCATGCGGCATTTAAGTTTCGTGAAGAATCGCACCACATCAAAGTACTAATCATAGTAGGACAACTTCACCCGATAATATTTGATACCCTGTTAAAAGCTAAGGATg           >  2:556780/1‑149 (MQ=255)
                                                                                                                                            cgcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGga         <  2:929667/149‑1 (MQ=255)
                                                                                                                                            cgcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGCCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACCCGATAACATTTGCTACCCAGTAAACGCCTATGGCTGga         >  1:1160562/1‑149 (MQ=255)
                                                                                                                                             gcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTACACGGCTATGGCTGgag        >  1:449852/1‑149 (MQ=255)
                                                                                                                                              cgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGgaga       <  2:879/149‑1 (MQ=255)
                                                                                                                                                 gTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACCGAACACGATAAGAATTGCTACCGACTAAACGGCTATGGCGGGagagac    >  1:58606/1‑147 (MQ=255)
                                                                                                                                                   tCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaa  >  2:596854/1‑149 (MQ=255)
                                                                                                                                                   tCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGcaaa  <  2:711036/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAA  >  NC_000913/3832650‑3832945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: