Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,414,249 G→C 5.1% intergenic (+58/‑26) yjfC → / → aidB ATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,414,2490GC 5.1% 253.9 / 3.7 92intergenic (+58/‑26)yjfC/aidBATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (51/34);  new base C (2/3);  total (55/37)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCG  >  NC_000913/4414103‑4414395
                                                                                                                                                  |                                                                                                                                                  
atgaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGAc                                                                                                                                                  >  2:609088/1‑149 (MQ=255)
 tgaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACt                                                                                                                                                 >  1:992345/1‑149 (MQ=255)
 tgaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACt                                                                                                                                                 <  1:1136389/149‑1 (MQ=255)
 agaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGAATGACATTGCTGGATAAGAATGTTTAAACAATCTCTTTCTGTAATGAAACCATGGCAGTGACCCTCCTAATGCTTACt                                                                                                                                                 >  1:992346/2‑149 (MQ=255)
   aaGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGc                                                                                                                                               <  2:617685/149‑1 (MQ=255)
    aGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGcc                                                                                                                                              >  2:138195/1‑149 (MQ=255)
    aGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCAATGGCAGTCACCATTAGTATGGTCACTGcc                                                                                                                                              <  1:1066297/149‑1 (MQ=255)
     gCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCa                                                                                                                                             >  2:748235/1‑149 (MQ=255)
     gCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCa                                                                                                                                             >  1:1156767/1‑149 (MQ=255)
      cGTGCGGAATGGGGATCCGTGAACATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTATCAATCTCTTTCTGACATGAATCCATGGCAGTCACCATTAGTATGGTCACTGCCAt                                                                                                                                            <  2:492599/149‑1 (MQ=255)
       gTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAtt                                                                                                                                           <  1:1088242/149‑1 (MQ=255)
        tGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTg                                                                                                                                          >  2:335392/1‑149 (MQ=255)
         gCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACCCCTCGCGATTCGTTCCGCATTACATTGCTGGATAAGAAGGTTGTGGCAATGTCTTTCTGGCATGAATCCATGGCTGTGACTATACTAATGGTCACTGCCATTgt                                                                                                                                         >  1:764928/1‑148 (MQ=255)
         gCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCATCTATTTAAGTCATGAATACATGGCAATGACCAAACTAATGGTGAATGCCAtttaa                                                                                                                                        >  1:651394/1‑146 (MQ=255)
              aTGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTTCTGGATAAGAATGTTTTAGCAATCTCATTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggag                                                                                                                                    <  2:666167/149‑1 (MQ=255)
              aTGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggag                                                                                                                                    <  1:552528/149‑1 (MQ=255)
                        gTGAAGATAACACACTGATCACCAACGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTACTGTCATGAATACATGACAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAg                                                                                                                          >  1:193751/1‑149 (MQ=255)
                           aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGc                                                                                                                       >  2:347010/1‑149 (MQ=255)
                           aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCGTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCAATTGATGGAGGGAGACACAGTGc                                                                                                                       >  2:201968/1‑149 (MQ=255)
                             gATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCAc                                                                                                                     >  1:508080/1‑149 (MQ=255)
                              aTAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACt                                                                                                                    <  2:1162570/149‑1 (MQ=255)
                                 acacacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGc                                                                                                                 >  1:190977/1‑149 (MQ=255)
                                 acacacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGATTAAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCTTTGATGGAGGAAGACACAGTGCACTGGc                                                                                                                 >  2:423136/1‑149 (MQ=255)
                                  cacacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCa                                                                                                                >  1:783697/1‑149 (MQ=255)
                                   acacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCaa                                                                                                               >  1:605141/1‑149 (MQ=255)
                                      cTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACt                                                                                                            <  2:508080/149‑1 (MQ=255)
                                      atGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTTCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACt                                                                                                            <  2:844768/148‑1 (MQ=255)
                                        gATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGCCACAGTGCACTGGCAAACTca                                                                                                          <  1:457566/149‑1 (MQ=255)
                                           caacAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCacac                                                                                                       <  2:1164058/146‑1 (MQ=255)
                                             ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCg                                                                                                     >  2:328698/1‑149 (MQ=255)
                                             ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCg                                                                                                     >  2:1184068/1‑149 (MQ=255)
                                             ccAAAGACACCTCACGTTTCGTTCCGCATAACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTTATGGAGGGAGACACAGTGAACTGGCAAAATCACACCg                                                                                                     >  1:1132312/1‑149 (MQ=255)
                                             ccAAAGACACCTCACGTTGCGGTCCGCAGTACAGTGCTGGTGAAGAATGTTGTAGCAAGCACTATCTGACATGAATCCATGGCAGTGACCAGACTTATGGTTACTGCCAATGATGGAGGGAGACACCGTGCACTGGCAAAAACACACCg                                                                                                     >  2:677420/1‑149 (MQ=255)
                                              cAAAGACTCCTCACGTTTCGTTCCGCATTACATTTCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                                    <  1:335666/149‑1 (MQ=255)
                                              cAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                                    >  1:68729/1‑149 (MQ=255)
                                              cAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAGTTCTGGAGGGAGCCACAGTGCACTGGCAAACTCACACCGt                                                                                                    >  1:1041852/1‑149 (MQ=255)
                                                 aGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGATTGTTTTAGCAATCTCTTTCTGTGATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGtttt                                                                                                 <  2:420422/149‑1 (MQ=255)
                                                    cacCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTaa                                                                                              <  2:432885/149‑1 (MQ=255)
                                                      ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATc                                                                                            <  2:783697/149‑1 (MQ=255)
                                                       cTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAAtaa                                                                                           >  1:1088904/1‑147 (MQ=255)
                                                        tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCaa                                                                                          <  2:546670/149‑1 (MQ=255)
                                                        tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCaa                                                                                          >  1:362404/1‑149 (MQ=255)
                                                              ttCGTTCCGCATTACATTGCTGGATTAGAATGTTTTAGCTATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCtata                                                                                    <  2:10955/149‑1 (MQ=255)
                                                              ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTAACACCGTTTTTAATCAACCtata                                                                                    >  1:755449/1‑149 (MQ=255)
                                                                  ttCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGATTCCATGGCAGTGACCATACTAATGGTGACTGCCGTTGATGGAGGGAGACCCAGTGCACTGGCAAACTCACACCGTTTTTATTCGACCTATTCCAt                                                                                >  2:83161/1‑149 (MQ=255)
                                                                  ttCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCAt                                                                                >  1:54160/1‑149 (MQ=255)
                                                                  ttCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCAt                                                                                >  1:432265/1‑149 (MQ=255)
                                                                     cggaTTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTTTCATGATTCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAGCATTaa                                                                             <  1:435422/146‑1 (MQ=255)
                                                                      gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTaaa                                                                            >  2:459804/1‑149 (MQ=255)
                                                                       cATTACATTGCTGGATTAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                           <  1:361509/149‑1 (MQ=255)
                                                                        aTTACATTGCTGGATAAGAATGTTTTAGAAATCTCTTTCTGTCATGAATCAATGGCAGTGACCATACTAATGCTGACTGCCATTGATGGAGGGAGACGCAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATa                                                                          >  1:570063/1‑149 (MQ=255)
                                                                                gCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTATTCAACCTATACAATTAAATAAAAGCAAc                                                                  >  2:157507/1‑149 (MQ=255)
                                                                                 cTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACAATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACt                                                                 >  1:213336/1‑149 (MQ=255)
                                                                                     aaaaGAATGTTTTAGGAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTtata                                                             <  2:335572/147‑1 (MQ=255)
                                                                                      tAAGAATGTTTTAGCAATATCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACATATAc                                                            <  2:68968/149‑1 (MQ=255)
                                                                                       aaGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATTGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTCGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATAcc                                                           >  2:674075/1‑149 (MQ=255)
                                                                                       aaGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATAcc                                                           >  2:855253/1‑149 (MQ=255)
                                                                                          aaTGTTTTAGCAAGCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGt                                                        <  2:1118075/149‑1 (MQ=255)
                                                                                              ttttAGGAATCTCTTTCTGTGATGAATCCATGGCAGTGACCATACTAATGGTGACTGGCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGa                                                    <  2:1025770/149‑1 (MQ=255)
                                                                                                ttAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATg                                                  >  2:675877/1‑149 (MQ=255)
                                                                                                 tAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATgg                                                 >  2:616689/1‑149 (MQ=255)
                                                                                                 tAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACAGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATgg                                                 <  1:609088/149‑1 (MQ=255)
                                                                                                 aaGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACAATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGGTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATgg                                                 <  2:357980/148‑1 (MQ=255)
                                                                                                  aGCAATCTCTTTCTGTCATGAATCAATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgc                                                <  2:669237/149‑1 (MQ=255)
                                                                                                   gCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGCGACACAGTGCCCTGGCAAACTCACCCCGTTTTTAAGCAACCTATACCTTTAAATAACAGCAACTTAGACCTGACTGATGgcg                                               >  2:135217/1‑149 (MQ=255)
                                                                                                        ctctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGctc                                          <  2:247791/149‑1 (MQ=255)
                                                                                                        ctcaTTCTGTCATGAATGCATGGCAGTGACCATACTAATGGTGACTGACATTGATGGAGGGAGACACAGTGAACTTGCAAACTCACACCGTATTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcgcgcgc                                          <  1:71405/149‑3 (MQ=255)
                                                                                                           tttCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCGGATGGCGCGCTCTGc                                       >  2:250468/1‑149 (MQ=255)
                                                                                                              cTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGaa                                    >  2:858924/1‑149 (MQ=255)
                                                                                                                   aTGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATCCCTGTCGGATGGCGCGCTCTGCGACGCGGt                               >  1:319904/1‑149 (MQ=255)
                                                                                                                     gAATCCATGGCAGTGACCATACTAATGGTGACTGCGATTGATGGAGGGAGCCACAGTGCACTGGCCAACTCACCCCGTTTTTACTCAACCTATACCATTAAATCACAGCCACTTATACATGTCTGATGGCGCGCTGTGCGACGCGGTac                             >  2:790623/1‑148 (MQ=255)
                                                                                                                     gAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTaa                             <  1:748235/149‑1 (MQ=255)
                                                                                                                     gAATCCATGGCAGTGACCATACTAATGGTGACTGCCATAGATGGAGGGAGACACAGTGCACTGCCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAAAACAGCAACTTATACCTATCTTATGGAGCGCGCTGCGAAGCTGTaa                             >  1:723705/1‑149 (MQ=255)
                                                                                                                       aTCCATGGCACTGACCATACTAATGGAGACTGCCATTGATGTAGGGAGACACAGTGCACTGGCAAACTCACACCGTATTTAATCAACCTAAACCTTTAAATAACAGCAACTTAAACCAGTATGATGGCGCGCTAAGCTAAGCGGTACcg                           >  2:266774/1‑149 (MQ=255)
                                                                                                                        tCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcgc                          >  1:1135808/1‑149 (MQ=255)
                                                                                                                         ccATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcgcg                         >  2:3899/1‑149 (MQ=255)
                                                                                                                          cATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGt                        <  2:757264/149‑1 (MQ=255)
                                                                                                                          cATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTATTTACTCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGAGGTAACGCGt                        >  1:284984/1‑149 (MQ=255)
                                                                                                                           aTGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTg                       >  2:211813/1‑149 (MQ=255)
                                                                                                                           aTGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTg                       <  1:138195/149‑1 (MQ=255)
                                                                                                                           aTGGCAGTCACCATTAGTATGGTCACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTg                       >  1:917522/1‑149 (MQ=255)
                                                                                                                            tGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGa                      >  2:620902/1‑149 (MQ=255)
                                                                                                                             ggCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGaa                     <  2:605141/149‑1 (MQ=255)
                                                                                                                              gCAGTGACCATACTAATGGTGACGGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAg                    <  2:918796/149‑1 (MQ=255)
                                                                                                                                 gTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTg                 <  2:791582/149‑1 (MQ=255)
                                                                                                                                    aCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctg              >  2:631260/1‑149 (MQ=255)
                                                                                                                                      cATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCATCCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggc            >  2:66155/1‑149 (MQ=255)
                                                                                                                                        tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggctg          <  1:211813/149‑1 (MQ=255)
                                                                                                                                             aTGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATa     >  1:280773/1‑149 (MQ=255)
                                                                                                                                             aTGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACAACGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATa     >  1:824759/1‑149 (MQ=255)
                                                                                                                                              tGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAg    <  2:1156767/149‑1 (MQ=255)
                                                                                                                                                gTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCg  >  1:1118246/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGCG  >  NC_000913/4414103‑4414395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: