Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,564,315 G→A 6.2% G125G (GGC→GGT)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,564,3150GA 6.2% 190.7 / 2.1 65G125G (GGC→GGT) yjiLputative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base G (35/25);  new base A (2/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGAC  >  NC_000913/4564177‑4564454
                                                                                                                                          |                                                                                                                                           
cAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCgcgc                                                                                                                                   <  2:682194/149‑1 (MQ=255)
 aGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCa                                                                                                                                  >  1:424977/1‑149 (MQ=255)
  ggCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCAt                                                                                                                                 <  2:777891/149‑1 (MQ=255)
     tGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGAATTTg                                                                                                                              >  2:687061/1‑149 (MQ=255)
        tCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGtca                                                                                                                           <  1:1116894/149‑1 (MQ=255)
           cTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGtcattc                                                                                                                        >  1:677453/1‑149 (MQ=255)
            ttCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGtcattca                                                                                                                       <  1:632364/149‑1 (MQ=255)
            cgcTGATTCACCAAACTATGTTCACTTAATCGTGCTGGCGTGTGGCGTGAAATTTTCTTTAATGATTTCGATTAGCTCGACGTTGGTGACTATCGTTCGTGAGATCCACTACAGTATATGCCCCTTTCCCGCCGCGCATTTGtcattca                                                                                                                       <  2:481405/147‑1 (MQ=255)
             tCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGtcattcat                                                                                                                      <  1:1082281/149‑1 (MQ=255)
              gtgaataaGCAAACACTGTGCACATACTAGTTATGGCGTGCGGCGTGACATTTTCGCTAATGCTTTCGAGTTGCGCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATtcatc                                                                                                                     <  1:619383/142‑1 (MQ=255)
               tGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATtcatca                                                                                                                    >  2:552920/1‑149 (MQ=255)
                         aaCACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGca                                                                                                          <  1:552920/149‑1 (MQ=255)
                            acTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAg                                                                                                       >  2:251909/1‑149 (MQ=255)
                                 gCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTAc                                                                                                  >  1:171835/1‑149 (MQ=255)
                                    caTACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTAGTCATTCATCAGGAAATCGCACAGGTTACCGt                                                                                               <  1:574512/149‑1 (MQ=255)
                                     aTACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGtc                                                                                              <  1:22580/149‑1 (MQ=255)
                                       aCTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGtcat                                                                                            <  2:1179945/149‑1 (MQ=255)
                                       aCTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGtcat                                                                                            <  2:1117858/149‑1 (MQ=255)
                                        cTCGTGTTGGCGTGCGGCGTGAGATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAATCGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGtcatc                                                                                           <  1:1135459/149‑1 (MQ=255)
                                        cTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGtcatc                                                                                           <  1:56366/149‑1 (MQ=255)
                                         tCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGtcatca                                                                                          <  1:402835/149‑1 (MQ=255)
                                           gTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGtcatcatc                                                                                        >  1:1003201/1‑149 (MQ=255)
                                                   gTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGaa                                                                                >  1:952128/1‑149 (MQ=255)
                                                       ggcgTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCAc                                                                            >  1:59836/1‑149 (MQ=255)
                                                        gcgTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAACCGCCCGGTGCCCGCCGCGCAGTTGTCATTCATCAGGCACTCGCACAGGTTACCGTCATCATCAAGCCGAATCACt                                                                           >  2:728203/1‑149 (MQ=255)
                                                        gcgTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTACCCGCCGCGCATTTGTCATTCATCAGGAAATCACACAGGTTACCGTCATCATCAAGCTGAATCACt                                                                           >  1:339953/1‑149 (MQ=255)
                                                           tGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTACCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTg                                                                        >  1:599013/1‑149 (MQ=255)
                                                            gACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAACCGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACATTGc                                                                       >  2:566844/1‑149 (MQ=255)
                                                                ttttCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTc                                                                   >  2:853303/1‑149 (MQ=255)
                                                                 tttCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTcc                                                                  >  1:529097/1‑149 (MQ=255)
                                                                     ggTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGac                                                              >  2:325973/1‑149 (MQ=255)
                                                                       tAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGACCGGTGCCCGCCGCGCATTTGTCATTCATAAGGAAATCGAACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCGTTacca                                                            >  2:670660/1‑149 (MQ=255)
                                                                        aaTGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGaccac                                                           <  1:251909/149‑1 (MQ=255)
                                                                             tGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCAGCAGGAAAACGCACAGGGTACCGTCCTCGTCAAGCAGAATCACGTTGCTGTCCTGACCACCGATg                                                      >  2:1137279/1‑149 (MQ=255)
                                                                              gTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCTCCGATGt                                                     >  1:84919/1‑149 (MQ=255)
                                                                               tCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTc                                                    >  1:228639/1‑149 (MQ=255)
                                                                                  aGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCt                                                                                   >  1:488763/1‑115 (MQ=255)
                                                                                  aGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCt                                                                                   <  2:488763/115‑1 (MQ=255)
                                                                                    ttGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTAACGTCATCATCAAGCTGAATCACATTGGTGTCCTGACCACCGATGTCGCTTa                                               >  2:553191/1‑149 (MQ=255)
                                                                                    ttGCTAGACCCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCGTGACCACCGAGGTCGCTTa                                               >  2:294170/1‑149 (MQ=255)
                                                                                      gCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCAATTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTAcc                                             >  2:458190/1‑149 (MQ=255)
                                                                                       cTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAACCGCCCGGTGCCAGCCGCGCATTTGTCATTCAGCAGGAAATCGCACAGGATACCGTCATGATCAAGATGAATCACTTTGCTGTCCTGACCACCGATGTCGCTTAccc                                            >  1:1057056/1‑148 (MQ=255)
                                                                                              cTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGt                                     >  1:436763/1‑149 (MQ=255)
                                                                                                 gTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGc                                  <  2:1157516/149‑1 (MQ=255)
                                                                                                 gTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGc                                  >  2:451199/1‑149 (MQ=255)
                                                                                                  tGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGACCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCt                                 <  1:1161121/149‑1 (MQ=255)
                                                                                                   gCCAAGCGTGCGCGAGATCACCTCCAGGAACCGCCCGGTGCCCGACGCGCATTTGACAGTCATCAGGAAATCGGACAGGTTACCGGCATCATCAAGCTGAATCAGTTTGGTGTCCTGCCCCCCGCTCTCGAGTACCGCGCGCGTTGCTg                                >  2:1014065/1‑149 (MQ=255)
                                                                                                   gCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGCCCACCGATGTCGATTACCGCGCGCGTTGCTg                                >  1:306182/1‑149 (MQ=255)
                                                                                                         cGTGCGCGAGATCACCTCCAGGAAACGCACGGTGCCCGCCGCGCATTTGTCATTCATCATGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATAAATTTGCTTTCCTGACCACCGATGTCTATTACCGCGCTCGTTGCTGTCGCaa                          <  2:357508/149‑1 (MQ=255)
                                                                                                             cgcgAGATCACCACCAGGAAACGCCCGGAGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAACCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGaaa                      <  2:64928/149‑1 (MQ=255)
                                                                                                              gcgAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAAc                     >  1:262217/1‑149 (MQ=255)
                                                                                                                 agaTCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTTCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCAGGCGCAAGAAACCGt                  >  1:643597/1‑149 (MQ=255)
                                                                                                                      aCCTCCAGGAAACGCCCGGTACCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGcc             <  2:599013/149‑1 (MQ=255)
                                                                                                                        cTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGcccc           >  2:374326/1‑148 (MQ=255)
                                                                                                                        cTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGcccc           >  1:163013/1‑148 (MQ=255)
                                                                                                                        cTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCa           >  1:23259/1‑149 (MQ=255)
                                                                                                                         tCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAACTCGCACAGGTTACCGTCATCATCAAGCTGAAACACTTTTCTGTCATGACCACCGATGTCGCTTACACCGCGCGTTGCTGGAGCAAGAAACCGTGCGCCCAg          >  1:711028/1‑149 (MQ=255)
                                                                                                                          ccAGGAAACGCCCGGTGGCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGc         <  2:873573/149‑1 (MQ=255)
                                                                                                                          ccAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGc         >  2:1046142/1‑149 (MQ=255)
                                                                                                                             ggAAACGCCCGGTGCCCGCCGCGCTTTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCg      >  1:1018770/1‑149 (MQ=255)
                                                                                                                              gAAACGCCCGGTGCCCGCCGCTCATTTGTCATTCATCAGGAAATCGCACAGGGTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGt     <  2:263606/149‑1 (MQ=255)
                                                                                                                              gAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGt     <  2:424977/149‑1 (MQ=255)
                                                                                                                               aaaCGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTg    <  2:540187/149‑1 (MQ=255)
                                                                                                                                aaCGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAACTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGGCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGa   <  2:293753/149‑1 (MQ=255)
                                                                                                                                 aCGCCCGGTACCCGCCGCGCATTTGTCATTCATCAGGAAATCACACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGAc  <  2:339953/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGAC  >  NC_000913/4564177‑4564454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑