Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,668 C→T 8.4% A112V (GCG→GTG)  chiP → chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,6680CT 8.4% 111.4 / 3.2 48A112V (GCG→GTG) chiPchitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base C (27/16);  new base T (2/2);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-01

CAAAACCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGC  >  NC_000913/708522‑708815
                                                                                                                                                  |                                                                                                                                                   
aaaaaCCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGt                                                                                                                                                   <  2:1005041/148‑1 (MQ=255)
  aaaCCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGttt                                                                                                                                                 <  1:1088768/149‑1 (MQ=255)
    accacccTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTAAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGGTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGtttga                                                                                                                                               >  2:1052524/1‑147 (MQ=255)
      caaccTTTCTCACTCCCCCTGGAATGCCAACCTCGATTTTCAGGCCGGTTATGCTGCTGATATGTTCGGCCTTCAAATTGCTCCGTATACGGCGAATGAAATGGCGGAAAACGGCGACAGCTCCCACCCCACCGAAATCGCGTTTTCaa                                                                                                                                             >  2:899724/1‑149 (MQ=255)
          cTTTCTCCCTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAg                                                                                                                                         >  1:621624/1‑149 (MQ=255)
          cTTTCTCACTCCACCTGGATTGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCGTGATATTGCTGCGTTTACGGCGATTGATATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAg                                                                                                                                         >  1:48624/1‑149 (MQ=255)
          cTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAATTGTCGGAAAACGGCGACAGCTCCCACCCGAACGAAATAGCGTTTTAAAAAAg                                                                                                                                         >  1:671056/1‑149 (MQ=255)
              ctcACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGtaat                                                                                                                                     >  1:571684/1‑149 (MQ=255)
              cgcACTCCACCTGTCATCCCCACCTCTCTTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATTGCGTTTTCAAAAAGtaat                                                                                                                                     <  2:783317/147‑1 (MQ=255)
                     cACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCt                                                                                                                              <  2:1094212/149‑1 (MQ=255)
                        cTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATg                                                                                                                           >  2:632536/1‑149 (MQ=255)
                           gAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTAGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAAAGGAGACAGCTCCCACCCGAAATAAATCGCGTTTTCAAAAAGTAATAATGCCTATGAcc                                                                                                                        >  1:1066445/1‑148 (MQ=255)
                                         aTTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGc                                                                                                          <  2:341494/149‑1 (MQ=255)
                                                 tCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGTCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAg                                                                                                  >  2:90004/1‑149 (MQ=255)
                                                      ttATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGTGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGta                                                                                             >  1:485192/1‑149 (MQ=255)
                                                      ttATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGTGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGta                                                                                             >  1:471065/1‑149 (MQ=255)
                                                       tATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATTGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGtat                                                                                            <  2:208031/149‑1 (MQ=255)
                                                             gctgATATGTTCGTCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCGCCCACCCGACCGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCt                                                                                      <  2:666523/149‑1 (MQ=255)
                                                             gctgAGATGTGCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGTAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCt                                                                                      <  2:187801/149‑1 (MQ=255)
                                                               tgATATGTTCGGCCTTGATATTGCTGCGTCTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGt                                                                                    <  2:752524/149‑1 (MQ=255)
                                                                  tatTTTCGTACTTTATATTGCTGCGATTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCTAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGtata                                                                                 <  2:856043/149‑1 (MQ=255)
                                                                      ttCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCTCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGc                                                                             >  1:691086/1‑149 (MQ=255)
                                                                        cGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAAAAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTAGAAGCATGAATAACGCTg                                                                           >  1:656328/1‑149 (MQ=255)
                                                                           ccTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGAAGAAGACTGGTCCGGCGACAAAAGCCGAATACGCCTGTATAAAGCTGAgg                                                                        >  2:748698/1‑149 (MQ=255)
                                                                            cTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGc                                                                       >  1:851629/1‑149 (MQ=255)
                                                                            cTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGACTATGACGAAGACAGGTCCGGCGACAAAAGCGGTATAATCATGTATAAAGCAGCGGc                                                                       >  1:844074/1‑149 (MQ=255)
                                                                            cTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCACACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACCATAGCGGTATAAGCCTGTATAAAGCTGCGGc                                                                       >  2:1041243/1‑149 (MQ=255)
                                                                                   ttGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAAttt                                                                <  2:932588/149‑1 (MQ=255)
                                                                                     gctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTGCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTaa                                                              <  2:458044/149‑1 (MQ=255)
                                                                                      ctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGACATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATACGCCTGTATAAAGCTGCGGCCAAATTTaaa                                                             >  1:158570/1‑149 (MQ=255)
                                                                                      ctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGTGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTaaa                                                             <  2:471065/149‑1 (MQ=255)
                                                                                      ctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGTGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTaaa                                                             <  2:485192/149‑1 (MQ=255)
                                                                                           tttACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCCCCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAACGCTGCGGACAAATTTAAATACgg                                                        >  1:911898/1‑149 (MQ=255)
                                                                                              aCGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGCCAAAAGCGGTATACGCCTGTATAAAGCTGCGGCCAAATTTATATACGGtgg                                                     >  1:854530/1‑147 (MQ=255)
                                                                                                ggCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCgg                                                   >  2:1151566/1‑149 (MQ=255)
                                                                                                 gCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTATCAAAAAGTAATAAAGCCTATGACTAAGACTGGTCCGGCGACAAAAGCTGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATAAGATCCGGt                                                  >  2:794357/1‑149 (MQ=255)
                                                                                                     ttGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAAAGAAATCGAGTTTTCAAAAAGTAATACAGCCTATGACGAAGACTGGTCCTGCGACAAATGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTgg                                              <  1:315469/149‑1 (MQ=255)
                                                                                                          aTGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAACGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTATGGGCGat                                         >  2:367041/1‑148 (MQ=255)
                                                                                                            ggCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAggg                                       >  2:1024095/1‑149 (MQ=255)
                                                                                                               ggAAAACGGCGACAGCTCCCACCCGAACGAACTCGCGTTTTCAAAAAGTAATAACCCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGATTGGGCGAGGGCAg                                    <  2:290355/149‑1 (MQ=255)
                                                                                                               ggAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGCCTGGTCCGGCGACAAAAGCGGTATAAGCCTGTTTAAAGCTGCGGCCAAAGTTAAATACGGTCCGGTTTGGGGGAGGGCAg                                    >  1:255769/1‑149 (MQ=255)
                                                                                                                  aaaCGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATTACGAAGACTTGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGAGGCCAAATTTAAATACGGTCCGTTTTGGGCGAGGGCAGGtt                                 >  2:776125/1‑149 (MQ=255)
                                                                                                                    actgCGACCTCTACCCCCCGAACGACAAGGCGCTATCACCACGTAATATCGCCTCTCACGAAGACTCGTCCCGCTACAAACGCCGTATAAGCCTGTATAAAGCTGCGGACAAATTTAAATACGGTCCGGGTTGGGCGAGGGCAGGTTAc                               <  2:901809/146‑1 (MQ=255)
                                                                                                                      ggCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAAAACGGTCCGGTTTGGGCGAGGGCAGGTTACAt                             >  1:595136/1‑149 (MQ=255)
                                                                                                                                     ccGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCCAACTGGTCa              >  2:1156355/1‑149 (MQ=255)
                                                                                                                                           gaaATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCt        <  2:571684/149‑1 (MQ=255)
                                                                                                                                            aaaTCGCGTTTTCAAACAGTAAAAAAGCCTCTGACGAAGACTGGTCCGGCGACAAACGCGGTATAAGCCTGTATACAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGGTACATTCAGCCAACTGGTCAAACGCTg       <  2:1087299/149‑1 (MQ=255)
                                                                                                                                               tCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCATGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCCGCCCACTGGTCAAACGCTGTTa    >  1:827094/1‑149 (MQ=255)
                                                                                                                                                 gcgTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGTATTGGGCGAGGGCAGGTTCCATTCCGACAACTTGTCAAACGCTGTTAgc  >  1:134462/1‑149 (MQ=255)
                                                                                                                                                 gcgTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCCCACTGGTCAAACGCTGTTAgc  >  2:70530/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAAAACCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGC  >  NC_000913/708522‑708815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑