Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	715,749	T→C	8.0%	V113A (GTT→GCT)	ybfP →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,749	0	T	C	8.0%	210.0 / 7.6	75	V113A (GTT→GCT)	ybfP	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (39/30);  new base C (3/3);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATA  >  NC_000913/715601‑715894                                                                                                                                                     |                                                                                                                                                  cGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGt                                                                                                                                                   >  2:603119/1‑149 (MQ=255)   tggtgATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATTCGGGttt                                                                                                                                                 >  2:522945/1‑149 (MQ=255)      tgATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGcc                                                                                                                                              <  2:777120/149‑1 (MQ=255)       gATAACTTACCTGATAATACCCGCCGCTTGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCa                                                                                                                                             <  2:47825/149‑1 (MQ=255)       gATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCa                                                                                                                                             <  1:986005/149‑1 (MQ=255)       gATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCa                                                                                                                                             >  1:47825/1‑149 (MQ=255)          aaCTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAggg                                                                                                                                          <  2:263500/149‑1 (MQ=255)            cTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCa                                                                                                                                        >  1:110142/1‑149 (MQ=255)               aCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGTGCAAGc                                                                                                                                     >  2:1124722/1‑149 (MQ=255)                 cTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGccc                                                                                                                                   >  1:327555/1‑149 (MQ=255)                  tGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAACAGAACTTACTTATCTTCATATTATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGCTTGCCCTGGCAAACCCg                                                                                                                                  <  1:63150/149‑1 (MQ=255)                  tGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCg                                                                                                                                  >  2:733781/1‑149 (MQ=255)                    ataataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGCTTGCCCTGGCAAACCCgca                                                                                                                                <  2:323941/149‑3 (MQ=255)                         aCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCg                                                                                                                           >  1:689931/1‑149 (MQ=255)                          cccGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGa                                                                                                                          <  2:1000511/149‑1 (MQ=255)                          cccGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGa                                                                                                                          >  1:1131040/1‑149 (MQ=255)                           ccgccgCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCCCAAGAAAAGGTCGGTTTAAATCCCGGATGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCTGCTCTCGAc                                                                                                                         >  1:767041/1‑149 (MQ=255)                           ccgccgCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGAc                                                                                                                         >  2:4678/1‑149 (MQ=255)                                    gCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCa                                                                                                                >  1:322099/1‑149 (MQ=255)                                      aGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAATAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTGTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAAt                                                                                                              <  2:737019/149‑1 (MQ=255)                                      aGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTAAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGCGTTTGCCAGGGCAAGCCCGGCAATCGACCGAAAGTCaaa                                                                                                              >  1:1057916/1‑148 (MQ=255)                                       gTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGGTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCCAGCACGGCACTCGACCGATAGTCAAtt                                                                                                             >  1:481513/1‑148 (MQ=255)                                          gTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAgg                                                                                                          >  2:137625/1‑149 (MQ=255)                                             aGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGCCCGATAGTCAATGAGGAtt                                                                                                       >  2:151248/1‑149 (MQ=255)                                               gCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTAt                                                                                                     >  2:45950/1‑149 (MQ=255)                                                cGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGGCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATg                                                                                                    <  1:527168/149‑1 (MQ=255)                                                       gAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTAAACCTCATTTTTTATGCGGGTTTGCCATTGCAAGCACGGAACTCGACCGATATTCAATGAGGATTATGGCAGCGa                                                                                             >  2:840288/1‑149 (MQ=255)                                                         aaatGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGaaa                                                                                           <  1:817085/149‑1 (MQ=255)                                                         aaaaGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGaaa                                                                                           <  1:1055267/149‑1 (MQ=255)                                                          aaaGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAg                                                                                          >  2:243046/1‑149 (MQ=255)                                                           aaGAACTTACTTATCATCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGc                                                                                         <  1:21475/149‑1 (MQ=255)                                                                     ttaTCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGTTTTGCCAGGGCAAGCCCGGCCCTCGACCGATAGTCAATGAGGATTATGGAAGCGAAAGCGGAATTTTTg                                                                               >  2:58676/1‑149 (MQ=255)                                                                     ttaTCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCAGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTg                                                                               >  2:739823/1‑149 (MQ=255)                                                                           tCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                         <  1:1170364/149‑1 (MQ=255)                                                                           tCATAATATGTCAGCACAAGAAAAGGGCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                         <  2:462297/149‑1 (MQ=255)                                                                             ataataTGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTAAGCGAAAGCGGATTTTTTGGCTCgagt                                                                       >  1:573629/1‑147 (MQ=255)                                                                              taataTGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCgatga                                                                      >  1:200721/1‑149 (MQ=255)                                                                               aataTGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCgatgat                                                                     <  1:733781/149‑1 (MQ=255)                                                                                atatGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTTGCTCGatgatg                                                                    >  2:1098103/1‑149 (MQ=255)                                                                                   tGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCAt                                                                 >  1:411821/1‑149 (MQ=255)                                                                                     tCAGCTCAAGAAAAGGTCGGTTTAAATCCAGGTTGGACATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGTCCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGt                                                               <  1:207893/149‑1 (MQ=255)                                                                                        gCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCgg                                                            >  2:1015340/1‑149 (MQ=255)                                                                                           caAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGat                                                         <  1:341577/149‑1 (MQ=255)                                                                                           caAGAAAACGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTATGCCAGCGCAAGCCAGGCACTCGACCGAAAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGAAGATGCATGTCGGGat                                                         >  1:352477/1‑149 (MQ=255)                                                                                              gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGattat                                                      >  1:1122896/1‑149 (MQ=255)                                                                                              gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGAAGCATGTCGGGattat                                                      >  1:348554/1‑149 (MQ=255)                                                                                                aaaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTg                                                    <  1:603119/149‑1 (MQ=255)                                                                                                 aaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGa                                                   >  1:532620/1‑149 (MQ=255)                                                                                                            aaaTCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCt                                        >  2:526975/1‑149 (MQ=255)                                                                                                                cccGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCa                                    >  2:77356/1‑149 (MQ=255)                                                                                                                  cGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGt                                  <  1:1124722/149‑1 (MQ=255)                                                                                                                  cGGTTGGCAATGCTACACCTCATTTTATATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGt                                  <  2:406789/149‑1 (MQ=255)                                                                                                                    gTTGGCAATGCTACACCTCATTTTTTATTCGGGTATGCCAGGGAAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTTTTGAGTTACGACGCTGCCAGTCa                                <  1:694336/149‑1 (MQ=255)                                                                                                                     ttGGCAATGCTACACCTCATTTTTTATGCGGGTTTGACATGGCAAGCCCGGGACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAg                               <  1:155191/149‑1 (MQ=255)                                                                                                                          aaTGCTACACCTCATTTTTTATGCGGGTTTGACATTGCAAGCCCGGCACTCGACAGATAGACAATGATGATTATGTAAGCTAAAGCGGATTTTTTGGATCGATGATGCATGTCGGGAATATTGAGTTACGACGCTGCCAGTCAGAAAAt                          >  2:697981/1‑149 (MQ=255)                                                                                                                           aTGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAAtt                         <  1:85508/149‑1 (MQ=255)                                                                                                                             gCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAACGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGc                       >  2:1058852/1‑149 (MQ=255)                                                                                                                               tACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCa                     >  2:302053/1‑149 (MQ=255)                                                                                                                               tACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCa                     <  2:1131040/149‑1 (MQ=255)                                                                                                                                   ccTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCaa                 <  1:137625/149‑1 (MQ=255)                                                                                                                                     tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGCCCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa               >  1:1008889/1‑149 (MQ=255)                                                                                                                                     tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa               <  2:689931/149‑1 (MQ=255)                                                                                                                                     tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa               >  1:491935/1‑149 (MQ=255)                                                                                                                                     tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa               >  2:980782/1‑149 (MQ=255)                                                                                                                                     tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAAGGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGGCGGGAGTTTTGGGTTACGCCGCGGCCAGTCAGCAAATTGccggaatca               >  2:217884/1‑142 (MQ=255)                                                                                                                                      cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCTATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa              <  2:926595/149‑1 (MQ=255)                                                                                                                                      cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa              <  1:77356/149‑1 (MQ=255)                                                                                                                                         tttttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg           <  1:45950/149‑1 (MQ=255)                                                                                                                                           tttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa         <  2:322099/149‑1 (MQ=255)                                                                                                                                            ttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa        <  2:532620/149‑1 (MQ=255)                                                                                                                                            ttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa        >  1:1005352/1‑149 (MQ=255)                                                                                                                                              tgccGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGCATATTTTGGCACGATGATGCATGTCGGGATTATTGAGTTACGACGCTGACAGTCAGAAAAATGCCAGCAAGAATTGAAAGc      >  2:490918/4‑149 (MQ=255)                                                                                                                                               tGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg     <  1:753870/149‑1 (MQ=255)                                                                                                                                                ccGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGa    >  1:481823/2‑149 (MQ=255)                                                                                                                                                 cGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt            >  1:940374/1‑140 (MQ=255)                                                                                                                                                 cGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt            <  2:940374/140‑1 (MQ=255)                                                                                                                                                  gggTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATa  >  1:271669/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATA  >  NC_000913/715601‑715894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑