Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,145,939 A→G 7.1% intergenic (+40/+72) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,145,9390AG 7.1% 207.8 / 5.7 70intergenic (+40/+72)rluC/yceF23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (32/33);  new base G (2/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

TGCAGCGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGC  >  NC_000913/1145791‑1146083
                                                                                                                                                    |                                                                                                                                                
tGCAGGGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATa                                                                                                                                                  <  1:25131/149‑1 (MQ=255)
tGCAGCGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATa                                                                                                                                                  <  1:160432/149‑1 (MQ=255)
  cAGCGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAg                                                                                                                                                <  1:691639/149‑1 (MQ=255)
     cGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCg                                                                                                                                             >  2:709020/1‑149 (MQ=255)
      gTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTTTGGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGt                                                                                                                                            <  1:1121181/149‑1 (MQ=255)
       ttGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGtt                                                                                                                                           >  1:1056178/1‑149 (MQ=255)
          aaGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCGTGATAAGGCGTTTAc                                                                                                                                        <  2:547574/149‑1 (MQ=255)
            ggatACTCATGCTGGGTCCGCTGTCGTGATGCGTATCGAATGTCCGATGGCTGAAGGTTTGAAGCGTTGTATGCAAAAGCTGCTTAACGCGCGCTAATACAACCATAAAAACCTGATAATTGGCGGTTGGCCTGATAAGGCGTTTAcgc                                                                                                                                      <  2:1112199/146‑1 (MQ=255)
               tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAAcgcctt                                                                                                                                   <  1:620401/149‑3 (MQ=255)
               tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTAcgccgc                                                                                                                                   <  2:372105/149‑1 (MQ=255)
                   cATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTCGGCCTGATGCGGCGTAAACGCCTTATCa                                                                                                                               <  2:389818/149‑1 (MQ=255)
                    aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                              <  2:756271/149‑1 (MQ=255)
                    aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                              >  1:601410/1‑149 (MQ=255)
                          ggACCGGTGCGGTGATGGGTATCGAATCTCCGATGGATGTATGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTTTTTTTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATAAGgccgcc                                                                                                                        <  2:291893/149‑1 (MQ=255)
                           gACCGGTGAGGTGATGCGTATCGAAGCTCCGATGTATGAACGTTTGAAGCGTTCTTTGCAAAAGCTGCGAAACGCGCGCTAATACAAGCATATACACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                       <  1:935040/149‑1 (MQ=255)
                           ccccGGTGAGGTGATGCGTAGCGAAGCTCCGATGGATGCAGGTTTGAAGCGTTGTTTGCAAAAGCGGCGTACCGCGCGCTAATCCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGTTAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                       <  1:1017861/147‑1 (MQ=255)
                              cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTCACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                    <  1:100373/149‑1 (MQ=255)
                              cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAACCCAGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                    >  1:900545/1‑149 (MQ=255)
                              cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAACGCCTTATCAGgccaaccgcc                                                                                                                    <  1:497779/149‑8 (MQ=38)
                                    ggtgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAtt                                                                                                              <  1:336500/149‑1 (MQ=255)
                                      tgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGc                                                                                                            >  1:722188/1‑149 (MQ=255)
                                               tCGAAGCTCCGATGGATGAAGGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATACGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGa                                                                                                   <  1:698117/149‑1 (MQ=255)
                                                  aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                >  2:322216/1‑149 (MQ=255)
                                                  aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                >  2:649758/1‑149 (MQ=255)
                                                  aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                >  2:317731/1‑149 (MQ=255)
                                                         gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                         >  2:65105/1‑149 (MQ=255)
                                                         gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGGTGGCCTGATAAGGCGTGTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                         <  2:776698/149‑1 (MQ=255)
                                                              atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                    <  1:180424/149‑1 (MQ=255)
                                                              atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                    >  2:844742/1‑149 (MQ=255)
                                                              atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                    >  1:849980/1‑149 (MQ=255)
                                                               tgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGgc                                                                                   >  1:857610/1‑149 (MQ=255)
                                                                       tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCATGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                           <  1:791187/149‑1 (MQ=255)
                                                                       tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                           <  1:709020/149‑1 (MQ=255)
                                                                          agccctctttGCAAAAGCGGCGTCACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCTGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                        <  1:852425/142‑1 (MQ=255)
                                                                          aGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                        <  1:158548/149‑1 (MQ=255)
                                                                                    gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                              >  1:654204/1‑149 (MQ=255)
                                                                                    gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                              >  2:533708/1‑149 (MQ=255)
                                                                                     cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                             <  2:866959/149‑1 (MQ=255)
                                                                                     cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                             >  1:786271/1‑149 (MQ=255)
                                                                                      aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAACCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCAGGCGCAAAAACCTATCCCATCAGCgg                                                            >  1:998541/1‑149 (MQ=255)
                                                                                      aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTCTCCCATCAGCgg                                                            >  1:1161525/1‑149 (MQ=255)
                                                                                      aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCgg                                                            >  2:349321/1‑149 (MQ=255)
                                                                                       aaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGAATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCggg                                                           <  2:930043/149‑1 (MQ=255)
                                                                                             gCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttc                                                     <  2:801661/149‑1 (MQ=255)
                                                                                                 aaCGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCACATCCGGCGCAAAAAACTAACCCATCAGCGGGTTTttccctt                                                 >  2:124407/1‑149 (MQ=255)
                                                                                                    gcgcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCTCATCAGTCCGCCAGCACCGATTGCAGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttcccttccc                                              >  2:212393/1‑149 (MQ=255)
                                                                                                      gcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGa                                            >  2:827739/1‑149 (MQ=255)
                                                                                                        gcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGATTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATACCATCAGCGGGTTTTTCCCTTCCCGACg                                          >  2:124145/1‑149 (MQ=255)
                                                                                                         cTAATACAAGTATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGt                                         <  1:626993/149‑1 (MQ=255)
                                                                                                          tAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                        >  1:359150/1‑149 (MQ=255)
                                                                                                            aTACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACTTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAAc                                      <  2:815374/149‑1 (MQ=255)
                                                                                                              aCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCTTCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATGCCCTGAGCGGGTTTTTCGCTTCCAGACGTTACAt                                    >  2:664653/1‑149 (MQ=255)
                                                                                                                aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACctct                                  >  1:846167/1‑149 (MQ=255)
                                                                                                                aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACATCt                                  >  1:128184/1‑149 (MQ=255)
                                                                                                                  gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCCCCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCGCATCAGCGGGTTTTTCCCTTCCCGACGTACCAGCTgc                                >  2:781533/1‑148 (MQ=255)
                                                                                                                  gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTCACATCTGa                                >  2:1108008/1‑149 (MQ=255)
                                                                                                                  gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                <  1:953617/149‑1 (MQ=255)
                                                                                                                  gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                <  1:528829/149‑1 (MQ=255)
                                                                                                                  gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                <  1:977435/149‑1 (MQ=255)
                                                                                                                       taAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAg                           <  1:196706/149‑1 (MQ=255)
                                                                                                                        aaaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATAAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAgc                          >  1:703415/1‑149 (MQ=255)
                                                                                                                         aaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAgcg                         >  1:1169196/1‑149 (MQ=255)
                                                                                                                           ccTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGcgcg                       >  1:202956/1‑149 (MQ=255)
                                                                                                                               aTAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTa                   <  1:549996/149‑1 (MQ=255)
                                                                                                                                tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAg                  <  1:315759/149‑1 (MQ=255)
                                                                                                                                 aaTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAGTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGc                 <  1:649758/149‑1 (MQ=255)
                                                                                                                                  aTGGGCGGTTGGCATGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg                <  2:857610/149‑1 (MQ=255)
                                                                                                                                     ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTa             <  1:65105/149‑1 (MQ=255)
                                                                                                                                       cGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTaaa           <  2:1056178/149‑1 (MQ=255)
                                                                                                                                            ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACTGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac      >  2:619617/1‑149 (MQ=255)
                                                                                                                                            ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTGCCCTTCCCGACGTAACATCTGACACAGCGGGAGTAGCGGTAacccaac      >  2:858071/1‑149 (MQ=255)
                                                                                                                                                tGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGAAGCGCCGTAACCACATCCGGCGCAAAAACCTATCCCATCCGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTACACCACCCCGc  >  1:723643/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TGCAGCGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGC  >  NC_000913/1145791‑1146083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: