Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,145,946 T→A 6.2% intergenic (+47/+65) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,145,9460TA 6.2% 196.9 / 2.9 73intergenic (+47/+65)rluC/yceF23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (36/31);  new base A (2/3);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

TTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGAT  >  NC_000913/1145798‑1146093
                                                                                                                                                    |                                                                                                                                                   
ttGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGtt                                                                                                                                                     >  1:1056178/1‑149 (MQ=255)
   aaGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCGTGATAAGGCGTTTAc                                                                                                                                                  <  2:547574/149‑1 (MQ=255)
     ggatACTCATGCTGGGTCCGCTGTCGTGATGCGTATCGAATGTCCGATGGCTGAAGGTTTGAAGCGTTGTATGCAAAAGCTGCTTAACGCGCGCTAATACAACCATAAAAACCTGATAATTGGCGGTTGGCCTGATAAGGCGTTTAcgc                                                                                                                                                <  2:1112199/146‑1 (MQ=255)
        tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAAcgcctt                                                                                                                                             <  1:620401/149‑3 (MQ=255)
        tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTAcgccgc                                                                                                                                             <  2:372105/149‑1 (MQ=255)
            cATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTCGGCCTGATGCGGCGTAAACGCCTTATCa                                                                                                                                         <  2:389818/149‑1 (MQ=255)
             aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                        <  2:756271/149‑1 (MQ=255)
             aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                        >  1:601410/1‑149 (MQ=255)
                   ggACCGGTGCGGTGATGGGTATCGAATCTCCGATGGATGTATGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTTTTTTTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATAAGgccgcc                                                                                                                                  <  2:291893/149‑1 (MQ=255)
                    gACCGGTGAGGTGATGCGTATCGAAGCTCCGATGTATGAACGTTTGAAGCGTTCTTTGCAAAAGCTGCGAAACGCGCGCTAATACAAGCATATACACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                                 <  1:935040/149‑1 (MQ=255)
                    ccccGGTGAGGTGATGCGTAGCGAAGCTCCGATGGATGCAGGTTTGAAGCGTTGTTTGCAAAAGCGGCGTACCGCGCGCTAATCCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGTTAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                                 <  1:1017861/147‑1 (MQ=255)
                       cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTCACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                              <  1:100373/149‑1 (MQ=255)
                       cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAACCCAGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                              >  1:900545/1‑149 (MQ=255)
                       cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAACGCCTTATCAGgccaaccgcc                                                                                                                              <  1:497779/149‑8 (MQ=38)
                             ggtgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAtt                                                                                                                        <  1:336500/149‑1 (MQ=255)
                               tgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGc                                                                                                                      >  1:722188/1‑149 (MQ=255)
                                        tCGAAGCTCCGATGGATGAAGGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATACGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGa                                                                                                             <  1:698117/149‑1 (MQ=255)
                                           aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                          >  2:649758/1‑149 (MQ=255)
                                           aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                          >  2:322216/1‑149 (MQ=255)
                                           aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                          >  2:317731/1‑149 (MQ=255)
                                                  gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                                   >  2:65105/1‑149 (MQ=255)
                                                  gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGGTGGCCTGATAAGGCGTGTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                                   <  2:776698/149‑1 (MQ=255)
                                                       atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                              <  1:180424/149‑1 (MQ=255)
                                                       atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                              >  1:849980/1‑149 (MQ=255)
                                                       atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                              >  2:844742/1‑149 (MQ=255)
                                                        tgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGgc                                                                                             >  1:857610/1‑149 (MQ=255)
                                                                tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCATGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                     <  1:791187/149‑1 (MQ=255)
                                                                tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                     <  1:709020/149‑1 (MQ=255)
                                                                   agccctctttGCAAAAGCGGCGTCACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCTGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                                  <  1:852425/142‑1 (MQ=255)
                                                                   aGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                                  <  1:158548/149‑1 (MQ=255)
                                                                             gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                        >  1:654204/1‑149 (MQ=255)
                                                                             gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                        >  2:533708/1‑149 (MQ=255)
                                                                              cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                       >  1:786271/1‑149 (MQ=255)
                                                                              cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                       <  2:866959/149‑1 (MQ=255)
                                                                               aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAACCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCAGGCGCAAAAACCTATCCCATCAGCgg                                                                      >  1:998541/1‑149 (MQ=255)
                                                                               aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTCTCCCATCAGCgg                                                                      >  1:1161525/1‑149 (MQ=255)
                                                                               aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCgg                                                                      >  2:349321/1‑149 (MQ=255)
                                                                                aaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGAATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCggg                                                                     <  2:930043/149‑1 (MQ=255)
                                                                                      gCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttc                                                               <  2:801661/149‑1 (MQ=255)
                                                                                          aaCGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCACATCCGGCGCAAAAAACTAACCCATCAGCGGGTTTttccctt                                                           >  2:124407/1‑149 (MQ=255)
                                                                                             gcgcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCTCATCAGTCCGCCAGCACCGATTGCAGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttcccttccc                                                        >  2:212393/1‑149 (MQ=255)
                                                                                               gcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGa                                                      >  2:827739/1‑149 (MQ=255)
                                                                                                 gcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGATTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATACCATCAGCGGGTTTTTCCCTTCCCGACg                                                    >  2:124145/1‑149 (MQ=255)
                                                                                                  cTAATACAAGTATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGt                                                   <  1:626993/149‑1 (MQ=255)
                                                                                                   tAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                  >  1:359150/1‑149 (MQ=255)
                                                                                                     aTACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACTTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAAc                                                <  2:815374/149‑1 (MQ=255)
                                                                                                       aCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCTTCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATGCCCTGAGCGGGTTTTTCGCTTCCAGACGTTACAt                                              >  2:664653/1‑149 (MQ=255)
                                                                                                         aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACctct                                            >  1:846167/1‑149 (MQ=255)
                                                                                                         aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACATCt                                            >  1:128184/1‑149 (MQ=255)
                                                                                                           gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCCCCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCGCATCAGCGGGTTTTTCCCTTCCCGACGTACCAGCTgc                                          >  2:781533/1‑148 (MQ=255)
                                                                                                           gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTCACATCTGa                                          >  2:1108008/1‑149 (MQ=255)
                                                                                                           gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                          <  1:977435/149‑1 (MQ=255)
                                                                                                           gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                          <  1:953617/149‑1 (MQ=255)
                                                                                                           gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                          <  1:528829/149‑1 (MQ=255)
                                                                                                                taAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAg                                     <  1:196706/149‑1 (MQ=255)
                                                                                                                 aaaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATAAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAgc                                    >  1:703415/1‑149 (MQ=255)
                                                                                                                  aaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAgcg                                   >  1:1169196/1‑149 (MQ=255)
                                                                                                                    ccTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGcgcg                                 >  1:202956/1‑149 (MQ=255)
                                                                                                                        aTAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTa                             <  1:549996/149‑1 (MQ=255)
                                                                                                                         tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAg                            <  1:315759/149‑1 (MQ=255)
                                                                                                                          aaTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAGTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGc                           <  1:649758/149‑1 (MQ=255)
                                                                                                                           aTGGGCGGTTGGCATGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg                          <  2:857610/149‑1 (MQ=255)
                                                                                                                              ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTa                       <  1:65105/149‑1 (MQ=255)
                                                                                                                                cGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTaaa                     <  2:1056178/149‑1 (MQ=255)
                                                                                                                                     ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACTGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac                >  2:619617/1‑149 (MQ=255)
                                                                                                                                     ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTGCCCTTCCCGACGTAACATCTGACACAGCGGGAGTAGCGGTAacccaac                >  2:858071/1‑149 (MQ=255)
                                                                                                                                         tGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGAAGCGCCGTAACCACATCCGGCGCAAAAACCTATCCCATCCGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTACACCACCCCGc            >  1:723643/1‑149 (MQ=255)
                                                                                                                                              aGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGtt       >  1:888531/1‑149 (MQ=255)
                                                                                                                                               ggCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGTGAAAAAAACTATCCCATCAGCGGGTTTTTCCCGTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTa      >  2:866274/1‑149 (MQ=255)
                                                                                                                                               ggCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGGCACCACATCCGGCGCAAAAAAGTAGCCGATCAGCGGGTTTTTCGATTCCCGACGTAACATCTGACACAGCGCGATAAGCGGTATACCAACCAGAGTGATa      >  2:711042/1‑149 (MQ=255)
                                                                                                                                                gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCCCAGCCGGCGCAAAAAACTATCCCAGAAGCGGGTTTTTACCTTCCCGACGTAACATAGTCCCCAGAGCGATTAGCGGTAAACCAACCAGCGTGTTat     >  2:643882/1‑148 (MQ=255)
                                                                                                                                                gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAGCGCGATTAGCGGTAAACCAACCCGCGTGTTAg     >  1:153423/1‑149 (MQ=255)
                                                                                                                                                gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAg     <  2:151241/149‑1 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAACCTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACAGCTGACACCGCGCGATGAGCGGTAAACCAACCAGCGTGGTAGGa   >  1:1124037/1‑149 (MQ=255)
                                                                                                                                                   tttACGCCGCATCTGGCGGGCAGCACCGATTGGCGGATGCGACGTATCCACATCCGGCGCAAAAAACTATCCCATGAGCGGGTTTTTCCCTTGCCGTCGTACCATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGAt  <  2:544538/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGAT  >  NC_000913/1145798‑1146093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: