Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,145,947 T→A 6.2% intergenic (+48/+64) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,145,9470TA 6.2% 193.7 / 2.9 73intergenic (+48/+64)rluC/yceF23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base A (2/3);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

AAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGAT  >  NC_000913/1145801‑1146093
                                                                                                                                                  |                                                                                                                                                  
aaGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCGTGATAAGGCGTTTAc                                                                                                                                                  <  2:547574/149‑1 (MQ=255)
  ggatACTCATGCTGGGTCCGCTGTCGTGATGCGTATCGAATGTCCGATGGCTGAAGGTTTGAAGCGTTGTATGCAAAAGCTGCTTAACGCGCGCTAATACAACCATAAAAACCTGATAATTGGCGGTTGGCCTGATAAGGCGTTTAcgc                                                                                                                                                <  2:1112199/146‑1 (MQ=255)
     tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAAcgcctt                                                                                                                                             <  1:620401/149‑3 (MQ=255)
     tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTAcgccgc                                                                                                                                             <  2:372105/149‑1 (MQ=255)
         cATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTCGGCCTGATGCGGCGTAAACGCCTTATCa                                                                                                                                         <  2:389818/149‑1 (MQ=255)
          aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                        >  1:601410/1‑149 (MQ=255)
          aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                        <  2:756271/149‑1 (MQ=255)
                ggACCGGTGCGGTGATGGGTATCGAATCTCCGATGGATGTATGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTTTTTTTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATAAGgccgcc                                                                                                                                  <  2:291893/149‑1 (MQ=255)
                 gACCGGTGAGGTGATGCGTATCGAAGCTCCGATGTATGAACGTTTGAAGCGTTCTTTGCAAAAGCTGCGAAACGCGCGCTAATACAAGCATATACACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                                 <  1:935040/149‑1 (MQ=255)
                 ccccGGTGAGGTGATGCGTAGCGAAGCTCCGATGGATGCAGGTTTGAAGCGTTGTTTGCAAAAGCGGCGTACCGCGCGCTAATCCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGTTAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                                 <  1:1017861/147‑1 (MQ=255)
                    cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTCACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                              <  1:100373/149‑1 (MQ=255)
                    cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAACCCAGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                              >  1:900545/1‑149 (MQ=255)
                    cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAACGCCTTATCAGgccaaccgcc                                                                                                                              <  1:497779/149‑8 (MQ=38)
                          ggtgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAtt                                                                                                                        <  1:336500/149‑1 (MQ=255)
                            tgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGc                                                                                                                      >  1:722188/1‑149 (MQ=255)
                                     tCGAAGCTCCGATGGATGAAGGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATACGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGa                                                                                                             <  1:698117/149‑1 (MQ=255)
                                        aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                          >  2:649758/1‑149 (MQ=255)
                                        aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                          >  2:317731/1‑149 (MQ=255)
                                        aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                          >  2:322216/1‑149 (MQ=255)
                                               gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                                   >  2:65105/1‑149 (MQ=255)
                                               gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGGTGGCCTGATAAGGCGTGTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                                   <  2:776698/149‑1 (MQ=255)
                                                    atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                              <  1:180424/149‑1 (MQ=255)
                                                    atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                              >  2:844742/1‑149 (MQ=255)
                                                    atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                              >  1:849980/1‑149 (MQ=255)
                                                     tgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGgc                                                                                             >  1:857610/1‑149 (MQ=255)
                                                             tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCATGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                     <  1:791187/149‑1 (MQ=255)
                                                             tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                     <  1:709020/149‑1 (MQ=255)
                                                                agccctctttGCAAAAGCGGCGTCACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCTGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                                  <  1:852425/142‑1 (MQ=255)
                                                                aGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                                  <  1:158548/149‑1 (MQ=255)
                                                                          gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                        >  1:654204/1‑149 (MQ=255)
                                                                          gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                        >  2:533708/1‑149 (MQ=255)
                                                                           cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                       <  2:866959/149‑1 (MQ=255)
                                                                           cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                       >  1:786271/1‑149 (MQ=255)
                                                                            aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAACCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCAGGCGCAAAAACCTATCCCATCAGCgg                                                                      >  1:998541/1‑149 (MQ=255)
                                                                            aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTCTCCCATCAGCgg                                                                      >  1:1161525/1‑149 (MQ=255)
                                                                            aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCgg                                                                      >  2:349321/1‑149 (MQ=255)
                                                                             aaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGAATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCggg                                                                     <  2:930043/149‑1 (MQ=255)
                                                                                   gCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttc                                                               <  2:801661/149‑1 (MQ=255)
                                                                                       aaCGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCACATCCGGCGCAAAAAACTAACCCATCAGCGGGTTTttccctt                                                           >  2:124407/1‑149 (MQ=255)
                                                                                          gcgcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCTCATCAGTCCGCCAGCACCGATTGCAGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttcccttccc                                                        >  2:212393/1‑149 (MQ=255)
                                                                                            gcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGa                                                      >  2:827739/1‑149 (MQ=255)
                                                                                              gcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGATTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATACCATCAGCGGGTTTTTCCCTTCCCGACg                                                    >  2:124145/1‑149 (MQ=255)
                                                                                               cTAATACAAGTATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGt                                                   <  1:626993/149‑1 (MQ=255)
                                                                                                tAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                  >  1:359150/1‑149 (MQ=255)
                                                                                                  aTACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACTTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAAc                                                <  2:815374/149‑1 (MQ=255)
                                                                                                    aCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCTTCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATGCCCTGAGCGGGTTTTTCGCTTCCAGACGTTACAt                                              >  2:664653/1‑149 (MQ=255)
                                                                                                      aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACctct                                            >  1:846167/1‑149 (MQ=255)
                                                                                                      aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACATCt                                            >  1:128184/1‑149 (MQ=255)
                                                                                                        gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCCCCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCGCATCAGCGGGTTTTTCCCTTCCCGACGTACCAGCTgc                                          >  2:781533/1‑148 (MQ=255)
                                                                                                        gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTCACATCTGa                                          >  2:1108008/1‑149 (MQ=255)
                                                                                                        gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                          <  1:977435/149‑1 (MQ=255)
                                                                                                        gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                          <  1:528829/149‑1 (MQ=255)
                                                                                                        gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                          <  1:953617/149‑1 (MQ=255)
                                                                                                             taAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAg                                     <  1:196706/149‑1 (MQ=255)
                                                                                                              aaaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATAAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAgc                                    >  1:703415/1‑149 (MQ=255)
                                                                                                               aaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAgcg                                   >  1:1169196/1‑149 (MQ=255)
                                                                                                                 ccTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGcgcg                                 >  1:202956/1‑149 (MQ=255)
                                                                                                                     aTAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTa                             <  1:549996/149‑1 (MQ=255)
                                                                                                                      tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAg                            <  1:315759/149‑1 (MQ=255)
                                                                                                                       aaTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAGTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGc                           <  1:649758/149‑1 (MQ=255)
                                                                                                                        aTGGGCGGTTGGCATGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg                          <  2:857610/149‑1 (MQ=255)
                                                                                                                           ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTa                       <  1:65105/149‑1 (MQ=255)
                                                                                                                             cGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTaaa                     <  2:1056178/149‑1 (MQ=255)
                                                                                                                                  ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACTGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac                >  2:619617/1‑149 (MQ=255)
                                                                                                                                  ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTGCCCTTCCCGACGTAACATCTGACACAGCGGGAGTAGCGGTAacccaac                >  2:858071/1‑149 (MQ=255)
                                                                                                                                      tGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGAAGCGCCGTAACCACATCCGGCGCAAAAACCTATCCCATCCGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTACACCACCCCGc            >  1:723643/1‑149 (MQ=255)
                                                                                                                                           aGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGtt       >  1:888531/1‑149 (MQ=255)
                                                                                                                                            ggCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGTGAAAAAAACTATCCCATCAGCGGGTTTTTCCCGTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTa      >  2:866274/1‑149 (MQ=255)
                                                                                                                                            ggCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGGCACCACATCCGGCGCAAAAAAGTAGCCGATCAGCGGGTTTTTCGATTCCCGACGTAACATCTGACACAGCGCGATAAGCGGTATACCAACCAGAGTGATa      >  2:711042/1‑149 (MQ=255)
                                                                                                                                             gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCCCAGCCGGCGCAAAAAACTATCCCAGAAGCGGGTTTTTACCTTCCCGACGTAACATAGTCCCCAGAGCGATTAGCGGTAAACCAACCAGCGTGTTat     >  2:643882/1‑148 (MQ=255)
                                                                                                                                             gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAGCGCGATTAGCGGTAAACCAACCCGCGTGTTAg     >  1:153423/1‑149 (MQ=255)
                                                                                                                                             gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAg     <  2:151241/149‑1 (MQ=255)
                                                                                                                                               gTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAACCTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACAGCTGACACCGCGCGATGAGCGGTAAACCAACCAGCGTGGTAGGa   >  1:1124037/1‑149 (MQ=255)
                                                                                                                                                tttACGCCGCATCTGGCGGGCAGCACCGATTGGCGGATGCGACGTATCCACATCCGGCGCAAAAAACTATCCCATGAGCGGGTTTTTCCCTTGCCGTCGTACCATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGAt  <  2:544538/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGAT  >  NC_000913/1145801‑1146093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: