Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,314,683	T→A	7.3%	intergenic (+25/+35)	ompW → / ← yciE	outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,314,683	0	T	A	7.3%	184.0 / 4.5	69	intergenic (+25/+35)	ompW/yciE	outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (28/35);  new base A (2/3);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

ATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAT  >  NC_000913/1314536‑1314828                                                                                                                                                    |                                                                                                                                                  aTGTCAGTGTGGTACATGGATATCGATACAACCGCCAATTATAAGCTGGGCGGTGCCCAGCCACACGATCGCGTACGACTCTATCCGTGGGTGTTTAGGGTCTCAGCAGGAGATCGTTTTTAATTCCGCACACAAACGACCCCGTAata                                                                                                                                                  >  1:880702/1‑149 (MQ=255)      aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgg                                                                                                                                             <  1:36683/149‑1 (MQ=255)         gtgGTACATGGATATCGATACCACCGCCAATTATAAGCTTGGCGTTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                                                                          <  1:613301/149‑1 (MQ=255)          tgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTc                                                                                                                                         <  1:347267/149‑1 (MQ=255)           ggTACATGGATTTCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcg                                                                                                                                        <  2:1170936/149‑2 (MQ=255)               cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                    <  1:441505/149‑1 (MQ=255)               aaTGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACTCCTCTATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCTCACAAAAACGACCCCGTATATTACGGGGTcgtttt                                                                                                                                    <  2:822662/148‑6 (MQ=255)                aTGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTGTTTTCTCAGCAGCATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgttttt                                                                                                                                   <  1:402832/149‑7 (MQ=255)                 tGGATATCGATACCACAGCCAATTATAATCTTGGCCGTGCACAGCAACACGATAGCTTACGCCTCGTTCCGTGGGTGTTTATGTTCTCAGCAGTATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                                                                  <  2:96026/149‑1 (MQ=255)                 tGGAAATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                                                                  <  1:750713/149‑1 (MQ=255)                  ggATATCGATACCACCGCCAATTATAATCTGGGCGGTGCACAGCAACACGATAGCGTCCGCCTCGATCCGTGGGTGTTTATGGTGGCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAGTATACGGGGTCATTATggc                                                                                                                                 >  1:766518/1‑148 (MQ=255)                      atCGATACAACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                                                                                             >  2:374892/1‑149 (MQ=255)                       tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                            <  1:816156/149‑1 (MQ=255)                         gATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACCCGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATAGTTTTTAATTCCGCACAAAAACGACACCGTAATATACGCGGTCAATAAGGACCTGGta                                                                                                                          >  2:238899/1‑149 (MQ=255)                          aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAAGATACGGGGTCAATAAGGACATGGtat                                                                                                                         >  2:168013/1‑149 (MQ=255)                             ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                                                                      >  1:1163029/1‑149 (MQ=255)                             ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAAATCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                                                                      <  1:841211/149‑1 (MQ=255)                               accGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAgg                                                                                                                    >  2:1045539/1‑149 (MQ=255)                                         ttaatgtGGGCGGTGCACAGATACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCGCAGCAGGATATCGTTTTTAAATCCTCACAAAAACGAACCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                                                                          <  2:100867/143‑1 (MQ=255)                                         ataAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                                                                          <  1:351683/149‑1 (MQ=255)                                         ataAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                                                                          <  2:395728/149‑1 (MQ=255)                                            aGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttcttc                                                                                                       <  1:85479/149‑1 (MQ=255)                                             gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGGCAATAAGGACATGGTATAAAGGGTATTATTTCTTCg                                                                                                      >  2:485614/1‑149 (MQ=255)                                              cTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGc                                                                                                     >  2:504215/1‑149 (MQ=255)                                                           aGCAACACGATAGCGTACGCCTCGATCCGGGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                        <  2:128760/149‑1 (MQ=255)                                                             cAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGt                                                                                      <  2:20442/149‑1 (MQ=255)                                                                cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc                                                                                   <  1:236182/149‑1 (MQ=255)                                                                   gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCCTCAGTTTCaga                                                                                >  2:1091722/1‑149 (MQ=255)                                                                   gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                                                                <  2:1161385/149‑1 (MQ=255)                                                                        cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGaa                                                                           <  2:862341/149‑1 (MQ=255)                                                                        cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGaa                                                                           <  1:870458/149‑1 (MQ=255)                                                                         gTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAAt                                                                          >  1:345590/1‑149 (MQ=255)                                                                            cGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTATGCCATCAGTTTCAGAGCGAATaaa                                                                       >  2:356379/1‑149 (MQ=255)                                                                             gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                                                                      <  1:1045539/149‑1 (MQ=255)                                                                               cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAAGGTATAAAGGGTATTATATCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaaa                                                                    >  1:531550/1‑149 (MQ=255)                                                                                tCGATCCTTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGTTCAATAAGGACATGGTTTAAAGGGTATTATTTCTTAGCTTCTACGCCAACAGTTTCAGAGCGAATTAAAAAt                                                                   >  2:700503/1‑149 (MQ=255)                                                                                tCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                                                                   >  2:244998/1‑149 (MQ=255)                                                                                  gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATATGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAttt                                                                 >  1:753426/1‑149 (MQ=255)                                                                                  gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAttt                                                                 <  2:343926/149‑1 (MQ=255)                                                                                    tCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtct                                                               <  2:11601/149‑1 (MQ=255)                                                                                       gTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAg                                                            <  1:510253/149‑1 (MQ=255)                                                                                           gtgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAGAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgt                                                        >  2:638386/1‑149 (MQ=255)                                                                                                 aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg                                                  <  2:422479/149‑1 (MQ=255)                                                                                                 aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg                                                  >  2:881671/1‑149 (MQ=255)                                                                                                    ttCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                               >  2:404129/1‑149 (MQ=255)                                                                                                    ttCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                               >  1:834060/1‑149 (MQ=255)                                                                                                     tctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAata                                              >  2:397660/1‑149 (MQ=255)                                                                                                       tcAGGAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATAtt                                            <  2:330557/149‑1 (MQ=255)                                                                                                        cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATATGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTc                                           <  1:587230/149‑1 (MQ=255)                                                                                                           cagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGa                                        <  2:980740/149‑1 (MQ=255)                                                                                                           cagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGa                                        >  2:22109/1‑149 (MQ=255)                                                                                                                  tCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                 >  1:907609/1‑149 (MQ=255)                                                                                                                             tCCGCAC‑AAACCGACCCCGTAATATACGTGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAttt                     <  1:1068667/149‑1 (MQ=255)                                                                                                                               cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTg                    <  1:238899/149‑1 (MQ=255)                                                                                                                                 cacaAAAACGTCCGCGTAATATACGGGGTCAATAATGACATGGTATAAAGTGTATTATTTCTTCGCTTCTACGCCATCAGTTTGAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                  <  2:802339/149‑1 (MQ=255)                                                                                                                                  acaAAAACGACCCCGTAATATAAGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGACATCAGTTTCAGAGAGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGACAGTCGGCCATTTGCtt                 >  1:859949/1‑149 (MQ=255)                                                                                                                                    aaaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt               >  2:160229/1‑149 (MQ=255)                                                                                                                                     aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc              <  1:873039/149‑1 (MQ=255)                                                                                                                                     aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc              <  1:296426/149‑1 (MQ=255)                                                                                                                                     aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTATCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc              <  1:413326/149‑1 (MQ=255)                                                                                                                                      tattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc             >  2:1081364/5‑149 (MQ=255)                                                                                                                                      aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc             >  2:554825/1‑149 (MQ=255)                                                                                                                                       aaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCt            <  2:345590/149‑1 (MQ=255)                                                                                                                                          gACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt         >  1:808388/1‑149 (MQ=255)                                                                                                                                           aCCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCGTGAtt        >  1:753322/1‑149 (MQ=255)                                                                                                                                               cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa    <  1:361425/149‑1 (MQ=255)                                                                                                                                               cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa    >  1:29674/1‑149 (MQ=255)                                                                                                                                               cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa    >  2:376494/1‑149 (MQ=255)                                                                                                                                                gTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTAAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTACTAATTTaaaa   >  1:1083865/1‑149 (MQ=255)                                                                                                                                                 tAATATACGGGGTCAATAAGGACATCGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAt  <  2:1166062/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAT  >  NC_000913/1314536‑1314828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑