Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,393,995 T→C 8.3% Y257H (TAC→CAC)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,393,9950TC 8.3% 133.7 / 3.7 48Y257H (TAC→CAC) mppAmurein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (27/17);  new base C (2/2);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CGGCAATGGCGCTTATGTTCTTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCG  >  NC_000913/1393847‑1394135
                                                                                                                                                    |                                                                                                                                            
cGGCAATGGCGCTTATGTTCTTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGtt                                                                                                                                              <  1:331387/149‑1 (MQ=255)
  gCAATGGCGCTTATGTTCTTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAATTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTAc                                                                                                                                            >  1:1161785/1‑149 (MQ=255)
       tgcgcTTATGTTCTTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAAATAATCAGGAATCCGCAGCCACTAAGCTTTACCTcgc                                                                                                                                       >  2:357273/2‑149 (MQ=255)
        gcgcTTATGTTATTAAATAGCGCGTAGTCAATGAAAATGTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTcgcg                                                                                                                                      <  2:88274/149‑1 (MQ=255)
            ttATGTTATTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGGGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCggggg                                                                                                                                  <  2:900443/149‑1 (MQ=255)
                  tCTTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTg                                                                                                                            <  1:20541/149‑1 (MQ=255)
                   cTTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGa                                                                                                                           <  1:1154758/149‑1 (MQ=255)
                     tAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACT‑CCAAAAGTGACCTTCCTGACAATTAATCAGGAATCCGCAGCCACAAAGCGTTACCTCGCGGGCGATAATGatat                                                                                                                        >  1:707934/1‑149 (MQ=255)
                                     aaTGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTccc                                                                                                         >  2:1020481/1‑149 (MQ=255)
                                     aaTGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTccc                                                                                                         >  1:684224/1‑149 (MQ=255)
                                        gAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGaa                                                                                                      >  1:986242/1‑149 (MQ=255)
                                        gAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTCACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGaa                                                                                                      >  1:424738/1‑149 (MQ=255)
                                        gAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTCACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGaa                                                                                                      >  1:419606/1‑149 (MQ=255)
                                          aaaaCTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGaaaa                                                                                                    >  2:851046/1‑149 (MQ=255)
                                          aaaaCTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGaaaa                                                                                                    >  1:499970/1‑149 (MQ=255)
                                           aaaCTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGaaaaa                                                                                                   >  1:282721/1‑149 (MQ=255)
                                              cTGGTCGTGGTACCGAATACCCATTATTGTGATAACGCCAAAACGGTACTGTAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAatat                                                                                                <  1:264860/149‑1 (MQ=255)
                                              cTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAatat                                                                                                <  2:2259/149‑1 (MQ=255)
                                                       gTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGaa                                                                                       <  2:12348/149‑1 (MQ=255)
                                                        tACCGAATTCCCATTATTGGGATAACGCCAATACTTTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAg                                                                                      <  1:811823/149‑1 (MQ=255)
                                                           cGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTg                                                                                   >  1:440297/1‑149 (MQ=255)
                                                             aaTACCCATTATTGGGATAACGCCAAAAAGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCCCCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGtt                                                                                 >  2:895016/1‑149 (MQ=255)
                                                                  ccATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAgg                                                                            >  2:409973/1‑149 (MQ=255)
                                                                                aatccAAAACGGTACTGCAATACGTGACCTTCCTGCCAATTACTCAGGAATCAGCAGACACAAAGCGTTACCTCGCGGGGTATATTGATATCACCGAATCCTTACCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAg                                                              <  2:373509/146‑1 (MQ=255)
                                                                                  gCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCCCCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGCGCAGGt                                                            >  1:278325/1‑149 (MQ=255)
                                                                                  gCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTCACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGt                                                            <  2:424738/149‑1 (MQ=255)
                                                                                  gCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTCACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGt                                                            <  2:419606/149‑1 (MQ=255)
                                                                                            tACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccg                                                  >  1:696164/1‑149 (MQ=255)
                                                                                            tACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccg                                                  >  1:154707/1‑149 (MQ=255)
                                                                                             aCTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccgc                                                 <  2:684224/149‑1 (MQ=255)
                                                                                               tGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTAAAcgccgccg                                               >  1:635091/1‑149 (MQ=255)
                                                                                               tGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACAGAATCATTCCCGAAAAATATTTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccgccg                                               >  2:1008234/1‑149 (MQ=255)
                                                                                                 cAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCa                                             >  2:221493/1‑149 (MQ=255)
                                                                                                    aaaGTGACCTTCCTGCCAATTAATCAGGCATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGGTTATACGCCGCCGCAGCt                                          <  1:1179760/149‑1 (MQ=255)
                                                                                                          aCCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGAc                                    <  2:282721/149‑1 (MQ=255)
                                                                                                            cTTCCTGCCAATTAATCAGGAAGCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTAGAACGATAATCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCt                                  <  1:179982/149‑1 (MQ=255)
                                                                                                                 tGCCAATTTATCAGGAATCCGCAGCCCCTAAGCGTTACCTCGCGGGGGATATTGATAGCACCGAATCCTTCCCGAAAAATATGAAACAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtattat                             >  2:414795/1‑149 (MQ=255)
                                                                                                                 tGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGCAGCTGTTGAAGGTTATTCCGGGGGAGGTTTATACGCCGCCGCCGCTCGGGCCCtattat                             >  2:419903/1‑149 (MQ=255)
                                                                                                                 tGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtattat                             >  2:455741/1‑149 (MQ=255)
                                                                                                                   ccatgtAATCAGGAATCCGCAGCGACTAAGCGTTACCTCGCGGGGGAAATTGATATCACCGAATCCTTACAGAAAAATATGTATGAGAAGATGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCGGCCGCTCGTGACCtattatta                           >  2:247058/6‑149 (MQ=255)
                                                                                                                             aGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGGTTATACGCCGCCGCAGCTCGGGACCTATTCTTATGCGTTTAac                 <  1:76745/149‑1 (MQ=255)
                                                                                                                               taaTCCGCAGCCACTAAGCTTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCATGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAacac               <  2:1150170/148‑1 (MQ=255)
                                                                                                                                 aTCCGCAGCCACTTAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGATTCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGc             >  2:511908/1‑149 (MQ=255)
                                                                                                                                    cGCAGCCACAAAGCGTTACCGCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATAATCCGGGGCAGGGTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCaaa          <  2:1013600/149‑1 (MQ=255)
                                                                                                                                     gCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCaaaa         >  1:984061/1‑149 (MQ=255)
                                                                                                                                      cAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCaaaaa        >  1:1038156/1‑149 (MQ=255)
                                                                                                                                          cACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGc    <  2:986242/149‑1 (MQ=255)
                                                                                                                                          cACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGGTTAACACGCAAAAAGGGc    >  2:491320/1‑149 (MQ=255)
                                                                                                                                            cTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCg  >  2:420803/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CGGCAATGGCGCTTATGTTCTTAAAGAGCGCGTAGTCAATGAAAAACTGGTCGTGGTACCGAATACCCATTATTGGGATAACGCCAAAACGGTACTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCG  >  NC_000913/1393847‑1394135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑